Supplemental enzyme in artificial diets for north african catfish larvae Clarias gariepinus Burchell, 1822

Supplemental enzyme in artificial diets with difference doses for African catfish Clarias gariepinus (initial weight of 0.0048±0.0001 g and total length 0.7633±0.0392 cm) were examined for 12 days feeding trial. The aim of this study was to analyze the effectiveness supplemental enzymes in diets for growth performance of catfish larvae. Tubifex sp. (control) and a multi enzyme was included at the level of 0.00 (ME0), 1.00 (ME1) and 2.00 g kg-1 (ME2 diet as a test diets. Each of the four experimental diets was randomly assigned to four plicate groups. Larva was allocated into 150 L aquaria at a density of 1350 larvae per aquarium. Data was analyzed using SPSS 20, followed by Duncan’s test. The result showed that the survival rate and condition factors were no statistical difference between experimental diets and control group. The highest final length, final weight, total food consumption, villi length and enzymes activity were recorded in the Control (P<0.05) and significantly different with other treatments. Feed efficiency’s control significantly different with other. Harvest size distribution, in the small size group (1-2 cm) did not show significantly different, in the medium size group (2-3 cm) and large size group (3-5 cm) the Tubifex sp. treatment were significantly different from the other treatments (P<0.05) whereas between ME0, ME1 and ME2 treatments were not significantly different. Among the supplemental enzyme in test diets, ME2 can improve weight and total length 6.25% and 13.4%, respectively than ME0 treatment. The results suggested that enzyme supplementation can improve intestinal structure and growth performance of catfish larvae. The supplemental enzyme in diets with doses up to 2 g kg-1 can increase intestinal villi’s length but have not been able to increase fish growth performance as use worm.

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Body weight changes, nutrient utilization and intestinal microflora of African catfish (Clarias gariepinus) fed Aloe barbadensis leaves

Nigerian Journal of Animal Production ◽

10.51791/njap.v43i1.2784 ◽

2021 ◽

Vol 43 (1) ◽

pp. 385-398

Author(s):

S. I. Ajibola ◽

S. O. Obasa ◽

A. K. Akintokun ◽

I. Abdulraheem

Keyword(s):

Weight Gain ◽

Growth Performance ◽

Intestinal Microflora ◽

Control Diet ◽

Clarias Gariepinus ◽

Nutrient Utilization ◽

Fish Growth ◽

African Catfish ◽

Weight Changes ◽

Aloe Barbadensis

Immuno-nutrition studies have shown that some nutrients are linked to the immunological status of fish. Growth performance, nutrient utilization and intestinal microflora were examined in Clarias gariepinus. The 120 C. gariepimus fingerlings (weight, 2.33±0.07g)were fed Aloe barbadensis leaves-paste supplemented diets in 40L freshwater-filled plastic tanks ad libitum twice daily for 12 weeks. The experimental diets containing 40% crude protein were supplemented with three concentration of A. barbadensis leaves-paste: ABL1-1%; ABL2-2%; ABL3-3% and control-0%. Mean weight gain (MWG) and percentage weight gain (PWG) increased (p<0.05) as the concentration of A. barbadensis increased. MWG (17.95±0.78) and PWG (772.2±54.94) were highest in fish fed ABL3 and lowest MWG (11.92±1.16) and PWG (17.95±0.78) in fish fed control diet. Nutrients were better utilized among the diets supplemented group at different significant levels (p < 0.05). The highest value of ANPU was observed in ABL1 when compared to all other treatments including the control. Survival rate decreased as concentration of paste increased. There were no significant differences (p>0.05) in the total bacterial counts (TBC) in A. barbadensis leaves-paste supplemented diets and the control having the highest TBC (23.67 ±0.88 x 105 CFU/ml). Growth of total fungal counts (TFC) was not observed in ABLI. There was a reduction in TFC as the concentration increased in the other supplemented diets, and the control having the highest TFC (7.67 ± 0.44 x 105CFU/ml). The study concluded that inclusion of 1% A. barbadensis leaves-paste as supplement in the diet could effectively improve the growth performance, nutrient utilization and survival of cultured C. gariepinus. A. barbadensis leaves-paste could also reduce the microbial load of the fish.

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Growth performance of Clarias gariepinus fed varying dietary phosphorus levels

Research in Agriculture Livestock and Fisheries ◽

10.3329/ralf.v4i3.35100 ◽

2017 ◽

Vol 4 (3) ◽

pp. 215-220

Author(s):

Ezike Christopher Onyemaechi ◽

Chukwuka Doris Queen ◽

Echor Felix Okaliwe

Keyword(s):

Growth Performance ◽

Control Diet ◽

Clarias Gariepinus ◽

Palm Kernel ◽

Fish Growth ◽

Control Fish ◽

African Catfish ◽

Dietary Phosphorus ◽

Significant Difference ◽

And Control

One hundred and twenty (120) juveniles of African catfish Clarias gariepinus were obtained in May 2015 from a reputable Fish Farm in Enugu to the University Research Farm (latitude 074o North and 082o South, longitude 068o East and 076o West with annual mean temperature of 30oC). The fish were fed for 49 days from June – July 2015 with diets containing 4 different inclusion levels of phosphorus T1 [0.6% P], T2 [0.8% P], T3 [1.00% P], T4 [1.2% P] and T5 [0% P] which served as control diet, to determine optimum P requirement for fish growth at probability level of P < 0.05 between various treatments and control. Each diet was formulated to contain 40% crude protein composed of yellow maize, soybean cake, palm kernel cake and fish meal. Fish fed with graded levels of phosphorus were significantly higher (P < 0.05) than control in FW and MDWG. There was however no significant difference (P > 0.05) in FCR and PER between T1, T2, T4 and control but T3 was significantly (P < 0.05) better in FCR (0.33) than control (0.39). Similarly, T3 was significantly (P < 0.05) higher in PER than control. Fish in T3 had the best growth performance indicative that 1% P is the optimum requirement for the growth of C. gariepinus.Res. Agric. Livest. Fish.4(3): 215-220, December 2017

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Suitability of discarded cashewnut (Anacardium occidentale) meal as replacement of soybean meal (Glycine max) in the diet of juvenile African catfish Clarias gariepinus (Burchell, 1822)

Indian Journal of Fisheries ◽

10.21077/ijf.2019.66.3.89214-10 ◽

2019 ◽

Vol 66 (3) ◽

Cited By ~ 5

Author(s):

Stanley Iheanacho ◽

Emmanuel Ogueji ◽

Christiana Igberi ◽

Fred Avwemoya ◽

Akunna Amadi-Eke ◽

...

Keyword(s):

Growth Performance ◽

Soybean Meal ◽

Clarias Gariepinus ◽

Carcass Composition ◽

Intestinal Morphology ◽

Partial Replacement ◽

Control Group ◽

African Catfish ◽

Haematological Profile ◽

Fish Group

The search for alternative protein sources in aquaculture nutrition is still trending as the use of costly traditional feed stuffs in aquaculture diet formulation is no longer sustainable. The present study investigated the effects of partial and total dietary inclusion of cashewnut meal on growth, haematology, carcass composition, serum biochemistry and intestinal histology of juvenile African catfish Clarias gariepinus (Burchell, 1822). Soybean meal (SBM) was substituted with cashewnut meal (CM) at 0, 50 and 100% in formulated fish diets. A total of 135 juveniles of C. gariepinus (30.25± 1.02 g; 16.08±0.34 cm) were randomly assigned to three dietary treatments (0, 50 and 100% CM based diets) at a stocking density of 45 fish per treatment and further randomised into triplicates of 15 fish per replicate. The feeding trial lasted for 56 days and fish were fed twice daily at 5% body weight. Statistical analysis was carried out for all the parameters evaluated in this study. Fish group fed 50% CM based diet had the best growth performance and haematological profile when compared to 100% CM fish group and the control group. Insignificant changes in serum biochemical parameters were observed in CM fed fish groups when compared to the control. Histological examination of fish intestinal morphology revealed no adverse changes in the cellular structure of mucosal layer and villi in 50% CM fed fish, while mild histo-morphological changes were observed in 100% CM fed fish group. Significant increases in villi length and weight were observed in fish group fed CM based diets when compared to the control. The findings of the present study revealed that partial replacement (50%) of soybean with cashewnut meal improved growth performance and haematological profile of C. gariepinus.

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Evaluation of glutamine supplementation in the diet on the structure and function of the intestine and the growth performance of African catfish, Clarias gariepinus (Burchell 1822) juvenile

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v21i2.575 ◽

2021 ◽

Vol 21 (2) ◽

pp. 131-149

Author(s):

Ismail Rahmat ◽

Dedi Jusadi ◽

Mia Setiawati ◽

Ichsan Ahmad Fauzi

Keyword(s):

Growth Performance ◽

Clarias Gariepinus ◽

Maximum Growth ◽

Structure And Function ◽

Fish Growth ◽

Glutamine Supplementation ◽

African Catfish ◽

Fish Length ◽

Protease Enzyme ◽

And Function

A triplicate experiment was conducted to evaluate the effect of the diet supplemented with free glutamine (Gln) on intestinal structure and function, as well as the growth performance of African catfish Clarias gariepinus juvenile. The commercial feed was supplemented with Gln of either 0% (control), 0.7%, 1.4% or 2.1%. Fish measuring 2 ± 0.02 cm were stocked in 12 aquariums 50x40x35 cm filled with water at a volume of 50 L with a density of 2,000 fish m-2. Fish were cultured for 30 days and fed on the diets three times a day at satiation. Results showed that the growth rate and biomass of the fish at the end of the experiment had a quadratic response, with the maximum growth achieved at 0.7% Gln treatment. The response pattern of fish growth was in line with the distribution of fish length. Fish in 0.7% Gln treatment had number fish measuring 5-6 cm more than 12% less than other treatments, while fish measuring 7-8 cm were more than other treatments. Higher growth in the 0.7% Gln treatment correlated with longer villi, higher protein retention, and ultimately higher feed efficiency. Increased intake of Gln in the diet also caused an increase in intestinal protease enzyme activity, and accumulation of Gln in the liver, but did not increase the enzymes activity of the liver Superoxidase Dismutase (SOD). It can be concluded that feeding on a diet supplemented with 0.7% Gln can improve the structure and function of the intestine, as well as increase the target size of catfish juvenile production.

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Body weight changes, nutrient utilization and intestinal microflora of African catfish (Clarias gariepinus) fed Aloe barbadensis leaves

Nigerian Journal of Animal Production ◽

10.51791/njap.v43i2.725 ◽

2021 ◽

Vol 43 (2) ◽

pp. 385-398

Author(s):

S. I Ajibola ◽

S. O Obasa ◽

A. K Akintokun ◽

I Abdulraheem

Keyword(s):

Weight Gain ◽

Growth Performance ◽

Intestinal Microflora ◽

Control Diet ◽

Clarias Gariepinus ◽

Nutrient Utilization ◽

Fish Growth ◽

African Catfish ◽

Weight Changes ◽

Aloe Barbadensis

Immuno-nutrition studies have shown that some nutrients are linked to the immunological status of fish. Growth performance, nutrient utilization and intestinal microflora were examined in Clarias gariepinus. The 120 C. gariepinus fingerlings (weight, 2.33±0.07g) were fed with Aloe barbadensis leaves-paste supplemented diets in 40L freshwater-filled plastic tanks ad libitum twice daily for 12 weeks. The experimental diets containing 40% crude protein were supplemented with three concentration of A. barbadensis leaves-paste: ABL1–1%; ABL2–2%; ABL3–3% and control–0%. Mean weight gain (MWG) and percentage weight gain (PWG) increased (p<0.05) as the concentration of A. barbadensis increased. MWG (17.95±0.78) and PWG (772.2±54.94) were highest in fish fed ABL3 and lowest MWG (11.92±1.16) and PWG (17.95±0.78) in fish fed control diet. Nutrients were better utilized among the diets supplemented group at different significant levels (p < 0.05). The highest value of ANPU was observed in ABL1 when compared to all other treatments including the control. Survival rate decreased as concentration of paste increased. There were no significant differences (p>0.05) in the total bacterial counts (TBC) in A. barbadensis leaves-paste supplemented diets and the control having the highest TBC (23.67 ± 0.88 x 105CFU/ml). Growth of total fungal counts (TFC) was not observed in ABL1. There was a reduction in TFC as the concentration increased in the other supplemented diets, and the control having the highest TFC (7.67 ± 0.44 x 105 CFU/ml). The study concluded that inclusion of 1% A. barbadensis leaves-paste as supplement in the diet could effectively improve the growth performance, nutrient utilization and survival of cultured C. gariepinus. A. barbadensis leaves-paste could also reduce the microbial load of the fish.

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Medicinal Plant Feed Additives Enhanced Survivability and Growth Performance of Clarias gariepinus (African Catfish) against Bacterial Infection

Microbiology Research ◽

10.3390/microbiolres12040054 ◽

2021 ◽

Vol 12 (4) ◽

pp. 744-752

Author(s):

Adebisi Musefiu Tiamiyu ◽

Isaac Olufemi Olatoye ◽

Okunlade Akinsola Olayemi ◽

Temitope Cyrus Ekundayo ◽

Olufemi Bolarinwa Adedeji ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Mortality Rate ◽

Medicinal Plant ◽

Growth Performance ◽

Allium Sativum ◽

Clarias Gariepinus ◽

Feed Additives ◽

Control Group ◽

African Catfish ◽

Chromolaena Odorata

The growth performance and survivability enhancement potential of some medicinal plant feed additives for combatting Pseudomonad infections in Clarias gariepinus were evaluated. Three medicinal plants (5 g/kg Allium sativum, 10 g/kg Chromolaena odorata and 10 g/kg Talinum triangulare) were incorporated separately into a basic diet. Juvenile Clarias gariepinus (n = 150, 53.05 ± 0.23 g), randomised into four groups, were fed for 42 days. The control group was fed with a non-supplemented diet. Growth parameters were determined and thereafter ten fish from each group, randomly selected, were inoculated intraperitoneally with pathogenic Pseudomonas aeruginosa (0.2 mL culture containing 1.4 × 106 cfu/mL). Their survivability was observed for 7 days based on mortality rate and relative level of protection (RLP). Mean weight gains were higher in all treated groups and significantly higher (p < 0.05) in the group of fish fed with 5 g/kg Allium sativum diet compared with the control. The lowest mortality rate (20%) and highest RLP (75) was recorded in the group fed with10 g/kg Chromolaena odorata. The results suggest that medicinal plant feed additives enhanced growth and survival of the cultured Clarias gariepinus. The study recommends 5 g/kg Allium sativum and 10 g/kg Chromolaena odorata diet supplementations as an effective growth promoter and anti-Pseudomonas aeruginosa agent, respectively, for Clarias gariepinus production.

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Grading frequency affect the growth performance and intra−cohort cannibalism in African catfish (Clarias gariepinus, burchell, 1822) culture

SDRP Journal of Aquaculture Fisheries & Fish Science ◽

10.25177/jaffs.3.2.ra.10656 ◽

2020 ◽

Vol 3 (2) ◽

pp. 222-231

Author(s):

Elijah Oyoo-Okoth ◽

◽

Anthony Muthoni Mwangi ◽

Charles C. Ngugi ◽

Jumbe James Jumbe

Keyword(s):

Growth Performance ◽

Clarias Gariepinus ◽

Size Variation ◽

Fish Growth ◽

African Catfish ◽

Poor Growth ◽

Large Size ◽

Randomized Design ◽

Dead Fish ◽

Size Heterogeneity

Growth of African catfish (Clarias garipinus) is characterized by large size heterogeneity resulting in poor growth performance and cannibalism. Strategies that limit size heterogeneity in C. gariepinus culture are therefore advocated. This study determined the effects of grading frequency on the growth performance and cannibalism of C. gariepinus in tank culture system. Three treatments designated as G0 (no grading), G2 (grading every 2 weeks) and G4 (grading every four weeks) in a Completely Randomized Design (CRD) was applied in triplicate. The tanks were stocked with 200 fingerlings each. Sampling was conducted weekly to measure length and weight of fish. Mortalities were recorded and dead fish observed under dissecting microscope to ascertain that it is due to cannibalism. Growth performance in terms of final mean weight, weight gain, specific growth rate and food conversion ratio were significantly (P < 0.05) affected by frequency of fish grading. The C. gariepinus graded every two weeks grew better than those graded every four weeks and were all above the no grading treatment. Significant changes in fish heterogeneity were observed after day 42. The size variation was significantly affected by grading frequency. The mean TL of fish graded every 2 weeks was the highest, followed by grading every four weeks and finally the no grading treatment was the lowest. Fish size heterogeneity (CV% and skewness) was consistently the highest in C. gariepinus in no grading treatment followed by fish where grading was done every four weeks while it was lowest in treatment where feeding was done every two weeks. Mortality owing to cannibalism was affected by grading frequency where highest cannibalism mortality occurred in C. gariepinus where there was no grading followed by grading every four weeks and intermediate at grading frequency every four weeks. Meanwhile other mortality by other causes such as such as wounds and suffocation in fish did not differ with grading frequency. Keywords: Cannibalism, Coefficient of variation, Fish grading, Fish growth, Growth heterogeneity

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The Effect of Three Different Feed Types on Growth Performance and Survival of African Catfish Fry (Clarias gariepinus) Reared in a Hatchery

ISRN Zoology ◽

10.5402/2012/861364 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Safina M. Musa ◽

Christopher Mulanda Aura ◽

Charles Chege Ngugi ◽

Rodrick Kundu

Keyword(s):

Growth Performance ◽

Lake Victoria ◽

Clarias Gariepinus ◽

Strong Relationship ◽

Quality Parameters ◽

African Catfish ◽

Nile Perch ◽

Total Length ◽

Maize Bran ◽

Food Fish

African catfish Clarias gariepinus is cultured as food fish, used to control overpopulation in tilapia ponds, and is grown as baitfish for the Lake Victoria Nile Perch fishery. Since the demand for Clarias gariepinus fingerlings is high, many farmers wish to specialize in fingerling production. In this study, the performance of three dietary feed types: freshwater rotifers with Artemia nauplii, freshwater rotifers with fish meal, and freshwater rotifers with maize bran, on the growth performance of African catfish fry, were done in a hatchery and they were raised in 9 glass tanks for a period of 21 days. Pearson’s correlation showed no relationship (r=0.1; P>0.05) between growth and water quality parameters, but indicated a strong relationship between survival rate and total length between treatments (r=0.85; P=0.02). Mean total length (TL) after 21 days were (A=15.003), (B=12.964), and (C=11.90) in mm for Treatments A, B, and C, respectively. Treatment A had the highest specific growth rate (SGR) of 6.475% day 1 followed by B (5.5320% day−1) and C (4.960% day−1). Results from this study demonstrate that after the 7 days of feeding using rotifers, Artemia is the best feed for increasing growth of Clarias gariepinus.

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Effect of Humic Substances as Feed Additive on the Growth Performance, Antioxidant Status, and Health Condition of African Catfish (Clarias gariepinus, Burchell 1822)

Animals ◽

10.3390/ani11082266 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2266

Author(s):

Markéta Prokešová ◽

Milena Bušová ◽

Mahyar Zare ◽

Hung Quang Tran ◽

Eliška Kučerová ◽

...

Keyword(s):

Growth Performance ◽

Humic Substances ◽

Biochemical Parameters ◽

Antioxidant Status ◽

Clarias Gariepinus ◽

Feed Additive ◽

African Catfish ◽

Growth Promoter ◽

Total N ◽

Positive Effects

In the present study, a possible nature immunostimulant and growth promoter—humic substances (HS) originating from Siberian leonardite mineraloid—were tested on juvenile Clarias gariepinus performance. Feed additive was applied onto commercial pelleted feed at four HS levels—0, 1, 3, and 6% w/w (HS0, HS1, HS3, HS6, respectively). Diets were tested in five repetitions (in total, n = 1800 individuals, mean body weight 28.1 ± 6.2 g) for 56 days. Growth and production parameters, fish condition and somatic indices, and overall mortality were evaluated after 14, 28, 42, and 56 days of exposure. Whereas, plasma samples were collected only after 0, 28, and 56 days, when fish health status was assessed with biochemical parameters (total proteins, TP; alanine aminotransferase, ALT; aspartate aminotransferase, AST; lactate dehydrogenase, LDH; cholesterol, CHOL; triglycerides, TAG) and fish antioxidant status with glutathione (reduced glutathione, GSH; oxidized glutathione, GSSG; glutathione ratio GSH/GSSG). Although a significantly positive effect of HS feed additive on growth performance was not found in the present study, moderately positive effects were found regarding biochemical parameters (ALT, AST, LDH, CHOL, and TAG) and antioxidants (GSH/GSSG ratio) that were improved especially in the HS3 group.

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Response of Clarias gariepinus to Allium sativum-based diet on growth performance and Staphylococcus aureus challenge infection

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v24i5.4 ◽

2020 ◽

Vol 24 (5) ◽

pp. 755-759

Author(s):

A.A. Nwabueze ◽

J.K. Ekelemu ◽

O.A. Owe

Keyword(s):

Staphylococcus Aureus ◽

Body Weight ◽

Growth Performance ◽

Allium Sativum ◽

Clarias Gariepinus ◽

Challenge Infection ◽

Fish Growth ◽

Fish Diet ◽

Feed Conversion ◽

Fish Samples

The response of Clarias gariepinus to Allium sativum on growth performance and as anti-bacterial agent in Staphylococcus aureus challenge infection was evaluated. A. sativum was included at 0% (control), 1.5%, 3.0%, and 4.5% in fish diet. Twenty fish samples each were randomly distributed into four tanks (T1, T2, T3 and T4). Fish were fed twice daily at 5% body weight for twelve weeks. All fish were challenged with 0.5ml of pure culture of S. aureus. A significant (P<0.05) increase in body weight, total length and standard length was observed. A. sativum at 3.0% inclusion promoted highest growth with feed conversion ratio (1.25-1.41), protein efficiency ratio (1.72-0.56), specific growth rate (1.92-2.09), condition factor (0.83-1.99) and survival rate (60-95). Fish-fed A. sativum diet showed that 4.5% inclusion had the least S. aureus activity. Bacteria load significantly (P<0.05) decrease at week 12. Culture water had dissolved oxygen of 4.0 - 4.2 mg/L, pH 6.0 - 7.0 and temperature of 26.0-28.0oC. This study has shown that A. sativum supplemented diets enhanced growth of C. gariepinus and functioned as antibacterial agent against S. aureus. A. sativum at 3.0% inclusion in diet is recommended for better fish growth and 4.5% A. sativum for antibacterial action. Keywords: Fish, herb, growth, infection

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