scholarly journals Efficacy of Cyombopogon Citratus Extract Against Enterococcus Faecalis

2021 ◽  
Vol 7 (2) ◽  
pp. 357-363
Author(s):  
Tessya Nitulo Permatasari Lahagu ◽  
Huynh Diem An ◽  
Cindy Denhara Wijaya ◽  
Mellisa Sim
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Average Diameter ◽  
Endodontic Treatment ◽  
Test Method ◽  
Cymbopogon Citratus ◽  
Pulp Tissue ◽  
Root Canals ◽  
One Way Anova

Endodontic treatment refers to the act of removing infected pulp tissue and formingand reforming the root canals to prevent bacteria from entering the root canals.Microbial infection in the root canal or in the periapical area is a factor that can leadto failure of root canal treatments. Endodontic treatment failure can be caused thebacteria Enterococcus faecalis. The irrigation agent commonly used is sodiumhypochlorite. Sodium hypochlorite in large concentrations can be toxic to tissues.Lemongrass plant (Cymbopogon citratus) is a natural ingredient that can be used asan antibacterial agent. The aims of study to determine the comparison of theantibacterial effectiveness of lemongrass plant extracts (Cyombopogon citratus (DC).Staph) concentrations of 25%, 50%, 75%, 100% with 2.5% sodium hypochlorite ininhibiting the growth of the bacterium Enterococcus faecalis. Lemongrass plantextracts Cyombopogon citratus (DC). Staph) made by maceration method andconverted into 25%, 50%, 75%, and 100%. The bacterial test method used the agardiffusion test (Kirby-Bauer test) to see the effectiveness of antibacterialsThe result ofthe one way ANOVA statistical test resulted in significance value of p=0,001 (p<0,05)so that means there was differences in the average diameter of inhibitory lemongrassplant extracts (Cyombopogon citratus (DC). Staph) concentrations of 25%, 50%,75%, 100% with 2.5% sodium hypochlorite in inhibiting the growth of the bacteriumthe results are 13.68 ± 1.34 mm, 16.08 ± 0.87 mm, 16.33 ± 0.35 mm, 17.78 ± 0.93mm and 7.83 ± 0.44 mm and reinforced by the posthoc Tukey HSD test. Theconclusion from the study that there is a difference between the antibacterialeffectiveness of lemongrass plant extract (Cyombopogon citratus (DC). Staph)concentrations of 25%, 50%, 75% and 100% with 2.5% sodium hypochlorite ininhibiting the growth of the bacterium Enterococcus faecalis.

scholarly journals Retrospective Assessment of Healing Outcome of Endodontic Treatment for Mandibular Molars with C-shaped Root Canal

2017 ◽  
Vol 18 (7) ◽  
pp. 591-595
Author(s):  
Kishore Kumar Majety ◽  
Basanta Kumar Choudhury ◽  
Anika Bansal ◽  
Achla Sethi ◽  
Jaina Panjabi
Keyword(s):  
Root Canal ◽  
Healing Process ◽  
Complete Healing ◽  
Endodontic Treatment ◽  
Root Canal Treatment ◽  
Pulp Tissue ◽  
Root Canal Therapy ◽  
Root Canals ◽  
Mandibular Molars ◽  
Retrospective Assessment

ABSTRACT Introduction A thorough knowledge of the anatomic morphology of the root canal system is necessary for the long-term success of the root canal therapy. The occurrence of C-shaped root canal configuration is one such variation. Achievement of favorable prognosis after commencing root canal therapy in such teeth is one of the challenges imposed on the endodontist. Hence, we evaluated the healing occurring after endodontic therapy in patients with C-shaped root canals in mandibular molars. Materials and methods The present study was conducted in the Department of Conservative Dentistry of the institution and included assessment of all the patients who underwent root canal treatment of the mandibular first and second molars. Endodontic therapy was performed in all the cases by experienced endodontist. Final postoperative radiographs were taken. Recording of the data of the personal and clinical details of a total of 250 patients was done. All the clinical and radiographic details of the patients, such as tooth location in the jaw, presence or absence of C-shaped canals, status of the pulp tissue, presence or absence of the fractures, and other details of the patients were recorded. Radiographic and clinical examination of the tooth of the patients was done during the baseline visit and further during the follow-up visits. The presence of C-shaped root canals was confirmed using the radiographs. Periapical index (PI) scoring system was used. Categories defined for enlisting the healing after the root canal treatment with the assessment of the PI score. All the results were analyzed by Statistical Package for the Social Sciences software. Results Vital pulp tissue was encountered in majority of the cases. C-shaped root canal configuration was observed in 40% of the cases included in the present study. After completion of the endodontic therapy, complete crown placement was done in only 22% of the cases. In cases of vital teeth with C-shaped root canals configuration, most of the teeth showed complete healing. Significant results were obtained while comparing the complete coverage crown parameter in relation to the healing process in teeth with C-shaped root canals. Conclusion In the present study, no significant effect of the C-shaped root canal configuration was found on the healing rate of the endodontic therapy in mandibular molars. Clinical significance Meticulous endodontic therapy with special techniques should be done while preparing teeth with C-shaped root canals. How to cite this article Bansal A, Parihar AS, Sethi A, Majety KK, Panjabi J, Choudhary BK. Retrospective Assessment of Healing Outcome of Endodontic Treatment for Mandibular Molars with C-shaped Root Canal. J Contemp Dent Pract 2017;18(7):591-595.

scholarly journals Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Tariq S. Abuhaimed ◽  
Ensanya A. Abou Neel
Keyword(s):  
Bond Strength ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Endodontic Treatment ◽  
Antibacterial Action ◽  
Adhesive Resin ◽  
Root Canals ◽  
Adverse Action ◽  
Complex Anatomy ◽  
Halogenated Compound

Effective shaping and cleaning of root canals are essential for the success of endodontic treatment. Due to the complex anatomy of root canal spaces, the use of various instrumentation techniques alone is not effective in producing bacteria-free root canal spaces. Irrigation, disinfectants, rinses, and intervisit medications are used in conjunction with the mechanical instrumentation to ensure the success of endodontic treatment. Sodium hypochlorite (NaOCl), a halogenated compound, is routinely used to irrigate the root canal during endodontic treatments. NaOCl has been known for its antibacterial action, proteolytic and dissolution capacity, and debridement properties. NaOCl, however, can alter the composition of dentin and hence its interaction with the adhesive resins used to bond the restorative materials to treated dentin. This review therefore covers in depth the action of NaOCl on dentin-adhesive resin bond strength including both enhancement and reduction, then mechanisms proposed for such action, and finally how the adverse action of NaOCl on dentin can be reversed.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2020 ◽  
Vol 11 (3) ◽  
pp. 243-248
Author(s):  
Mohammad Asnaashari ◽  
Niloufar Kooshki ◽  
Mohammad Mahdi Salehi ◽  
Saranaz Azari-Marhabi ◽  
Hedieh Amin Moghadassi
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
New Technologies ◽  
Irrigation System ◽  
Bacterial Colony ◽  
Root Canals ◽  
Infected Root ◽  
Activation System

Introduction: Enterococcus faecalis is a resistant bacterium which is the most abundant species in infected root canals. Photodynamic therapy (PDT) is a method for killing the bacteria with active Oxygen radicals generated in a photosensitizer when exposed to centralized light. Furthermore, as a new method of canal disinfection, a variety of irrigation activation systems have been introduced, one of which is GentleFile (GF) with rotary movements and spiral effects for antibacterial action. This study aimed to compare the effectiveness of the two mentioned methods when used with and without Sodium Hypochlorite in eliminating E. faecalis from infected root canals. Methods: Fifty-eight uniradicular teeth were randomly divided into 4 experimental groups of 14. Two specimens were selected for later scanning electron microscopy in order to screen the procedure steps. In each experimental group, 10 samples were selected to be treated with GF or PDT; 3 of them were selected as positive controls and the other one sample was chosen as a negative control. Experimental groups were as follows: (1) Irrigation activation system, (2) Irrigation activation system + sodium hypochlorite, (3) PDT, and (4) PDT+ sodium hypochlorite. The specimens were then cultured for a bacterial colony count. Results: The decrease in the bacterial count after the treatment with the irrigation activation system was 99.8% (P=0.011) and when the system was used with sodium hypochlorite, it was 100% (P=0.001). The antibacterial effect of PDT was 90.08% (P=0.011) and it was 99.7% when PDT was combined with sodium hypochlorite (P=0.011). Conclusion: All four methods can be administered as complementary methods in root canal disinfection. According to the results of disinfection in the experimental groups of current study it is concluded that integration of new technologies such as activation irrigation system or PDT in Combination with NaOCl ameliorates disinfection of root canal and can provide several advantages in the endodontic outcome.

scholarly journals Evaluation of 4% Sodium Hypochlorite in eliminating Enterococcus faecalis from the Root Canal when Used with Three Irrigation Methods: An in vitro Study

2017 ◽  
Vol 18 (3) ◽  
pp. 214-217
Author(s):  
Vinisha Pandey ◽  
Harsh Priyank ◽  
Abhishek Bagul ◽  
Kishore Kumar Majety ◽  
Parul Verma ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Saline Solution ◽  
Brain Heart Infusion ◽  
Root Canals ◽  
Irrigation Methods ◽  
Group I ◽  
Significant Difference

ABSTRACT Introduction Endodontic treatment removes all pathogens, such as Enterococcus faecalis from pulp and root canals. The aim of this study is to assess the usefulness of sodium hypochlorite (NaOCl) in removing E. faecalis from the root canal used with three different irrigation methods. Materials and methods This study was conducted on freshly extracted maxillary incisors. After biomechanical preparation, root canals were injected with E. faecalis. Three groups were made which contained 30 teeth in each group; 2 mL of NaOCl solution was used for irrigation followed by agitation with K-files in group I; 2 mL of NaOCl solution was used for irrigation and ultrasonic agitation was done in group II. In group III, an alternate irrigation with NaOCl and 3% hydrogen peroxide was done. The fourth group (control) was irrigated with sterile saline solution. E. faecalis bacteria were sampled to the root canals with paper points and were transferred to tubes that contained 5 mL of brain heart infusion broth. Tubes were incubated and the presence of broth turbidity was suggestive of bacteria remaining in the root canal. Results All three groups showed no statistically significant difference. However, difference existed between experimental groups and control groups. Conclusion The author concluded that all three methods of application of NaOCl were effective in disinfecting the root canal than the saline solution. Clinical significance No single irrigant has 100% efficiency. Thus by this study, a best irrigating solution with maximum properties can be established. How to cite this article Priyank H, Pandey V, Bagul A, Majety KK, Verma P, Choudhury BK. Evaluation of 4% Sodium Hypochlorite in eliminating Enterococcus faecalis from the Root Canal when Used with Three Irrigation Methods: An in vitro Study. J Contemp Dent Pract 2017;18(3):214-217.

scholarly journals Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis

2016 ◽  
Vol 17 (8) ◽  
pp. 650-653 ◽  
Author(s):  
Jain Mathew ◽  
Reshmi George ◽  
Robin Theruvil ◽  
Tobin C Padavil ◽  
Lincy Tomy ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Root Canals ◽  
Root Canal Irrigants ◽  
Simarouba Glauca

ABSTRACT Aim To determine the antimicrobial effect of water extracts of leaves of Annona muricata and Simarouba glauca on Enterococcus faecalis using agar diffusion method. Materials and methods Dried leaves of A. muricata and S. glauca were powdered and extracted in a soxhlet apparatus. Enterococcus faecalis was grown overnight in Trypticase soy agar plates. About 10 μL of each extract was placed on agar plates and incubated overnight. The zone of inhibition was measured after 24 hours. About 1% sodium hypochlorite and distilled water were used as positive and negative controls. Results The leaf extract of A. muricata showed similar effectiveness as that of sodium hypochlorite, whereas the leaf extract of S. glauca showed only a slight reduction in growth of E. faecalis. Conclusion Leaf extract of A. muricata can be developed as an alternative to sodium hypochlorite for root canal irrigants. Clinical significance Success of endodontic treatment depends on complete disinfection of the root canals. Root canal irrigants have a major role in complete disinfection of the root canals. Chemical root canal irrigants are more or less toxic to the oral environment. In this study, naturally derived leaf extracts of A. muricata and S. glauca are compared with sodium hypochlorite for its effectiveness against E. faecalis – the most common pathogen found in the root canals. How to cite this article Mathew J, George R, Theruvil R, Padavil TC, Tomy L, Kurian A. Antibacterial Activity of Leaf Extract of Annona muricata and Simarouba glauca on Enterococcus faecalis. J Contemp Dent Pract 2016;17(8):650-653.

scholarly journals Comparison of Antimicrobial Activity of Sodium Hypochlorite (NaOCI) 2.5%, Microemulsion of Myrtus 10%, Microemulsion of Thyme 0.6% on the Enterococcus Faecalis After Root Canal Filling

2021 ◽  
Vol 24 (3) ◽  
pp. 424-437
Author(s):  
Forough Khodadadnejad ◽  
◽  
Majid Akbari ◽  
Fateme Abdolalian ◽  
Mahboube Daneshvar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Negative Control Group ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Control Group ◽  
Root Canal Filling ◽  
Anterior Teeth

Background and Aim: The success of endodontic treatment depends on the complete elimination of pathogenic bacteria inside the root canal. Enterococcus faecalis, a gram-positive, pathogenic, and anaerobic cocci, are the primary etiology of root canal infection in treated and untreated teeth and are highly associated with endodontic treatment failure. This study aimed to compare the antimicrobial activity of Sodium hypochlorite 2.5%, microemulsion of Myrtus 10%, and microemulsion of Thyme 0.6% on the E. faecalis after root canal filling. Methods & Materials: In this experimental study, 25 extracted single canal human anterior teeth were collected, and their crowns were cut. Then after washing and cleaning, and filling, the sterilization was done using an autoclave. Then, except 5 teeth (negative control group), the teeth were smeared with E. faecalis and then divided into 5 groups randomly represented the irrigation by Sodium hypochlorite 2.5%, microemulsion of Myrtus 10%, and microemulsion of Thyme 0.6%, with a positive control group and negative control group. The teeth were filled with gutta-percha and then incubated in an incubator for 90 days at a temperature of 37°C. Finally, the comparison of CFU in the groups due to abnormal data distribution was performed using the Kruskal-Wallis test. Ethical Considerations: The Ethics Committee of Arak University of Medical Sciences approved this study (Code: IR.ARAKMU.REC.1398.297). Results: According to the results of this study, the correlation between the groups of cultured teeth was not significant(P>0.05). The specimens washed with microemulsion of the Thyme 0.6% had the highest positive number of cultures; the specimens washed by Sodium hypochlorite 2.5% had the lowest number of cultures. Conclusion: This study showed that no statistically significant difference exists in their antimicrobial activity after root canal therapy after the use of different wash solutions.

scholarly journals A Comparative study to quantify the area of obturating material, sealer and voids in root canals obturated with Resilon/Epiphany and gutta-percha with different sealers

2014 ◽  
Vol 3 (1) ◽  
pp. 8-13
Author(s):  
M Vikram ◽  
N Shetty ◽  
VP Singh
Keyword(s):  
Comparative Study ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Endodontic Treatment ◽  
Filling Material ◽  
Bonferroni Correction ◽  
Medical Sciences ◽  
Root Canals ◽  
Gutta Percha ◽  
Ah Plus

Background: Complete obturation of the root canal with an inert filling material and creation of a fluid-tight seal are among the major goals of successful endodontic treatment. Methods: Extracted mandibular premolar teeth were decoronated, prepared in a crown-down fashion and irrigated with 2.5% sodium hypochlorite and 17% EDTA. The specimens were randomly assigned into 4groups (n=10/each) and obturated with gutta-percha and Resilon/Epiphanywith different sealers. Horizontal sections were obtained of the coronal, middle and apical third at levels of 14-mm, 8-mm and 2-mm from the apex respectively and were viewed under magnification. The area of obturating material, sealer and voids were calculated using Image Jsoftware. Statistical comparisons between the Resilon and gutta-percha groups were made with Kruskal-Wallis Test and intergroup comparisions were made by Mann-Whitney U test with Bonferroni correction. Results: The results of this study showed that root canals obturated with gutta-percha or Resilon with Epiphany as sealer had significantly less area of sealer plus voids and voids alone as compared to root canals obturated with gutta-percha and AH Plus as sealer or gutta-percha and GuttaFlow. Conclusion: From the results of this study it can be concluded that methacrylate resin-based sealer Epiphany will lead to a better obturation of the root canals and adhesive sealers hold a better prospective. Nepal Journal of Medical Sciences | Volume 03 | Number 01 | January-June 2014 | Page 8-13 DOI: http://dx.doi.org/10.3126/njms.v3i1.10342

scholarly journals Synergistic Antimicrobial Action of Chlorhexidine and Ozone in Endodontic Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Rita Noites ◽  
Cidália Pina-Vaz ◽  
Rita Rocha ◽  
Manuel Fontes Carvalho ◽  
Acácio Gonçalves ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Endodontic Treatment ◽  
Periapical Lesions ◽  
Root Canals ◽  
Single Root ◽  
Gaseous Ozone ◽  
Infected Root

Objectives. The aim of this study was to determine whether irrigation with sodium hypochlorite, chlorhexidine, and ozone gas, alone or in combination, were effective againstEnterococcus faecalisandCandida albicans; these are microorganisms frequently isolated from teeth with periapical lesions resistant to endodontic treatment.Material and Methods. 220 single root teeth, recently extracted, were inoculated withCandida albicansandEnterococcus faecalis. The formulations tested were sodium hypochlorite at 1, 3, and 5% chlorhexidine at 0.2% and 2% and ozone gas applied for different periods of time. The combination of sodium hypochlorite at 5% and chlorhexidine at 2%, with gaseous ozone, were also assessed. For the most active treatments the mechanism of action was assessed through flow cytometry.Results. Sodium hypochlorite, chlorhexidine, and gaseous ozone alone were ineffective in completely eliminating the microorganisms. The association of chlorhexidine at 2% followed by ozone gas for 24 seconds promoted the complete elimination ofCandida albicansandEnterococcus faecalis. Flow cytometry shows that ozone and chlorhexidine act differently, which could explain its synergic activity.Conclusions. This new disinfection protocol, combining irrigation with chlorhexidine at 2% and ozone gas for 24 seconds, may be advantageous when treating infected root canals.

scholarly journals Zona hambat ekstrak etil asetat daun kemangi (Ocimum basilicum) terhadap Enterococcus faecalis ATCC 29212Inhibition zone of ethyl acetate extract of basil leaves (Ocimum basilicum) against Enterococcus faecalis ATCC 29212

2021 ◽  
Vol 33 (1) ◽  
pp. 8
Author(s):  
Rahmi Wastri ◽  
Ame Suciati Setiawan ◽  
Dani Rizali Firman ◽  
Diani Prisinda ◽  
Fajar Fatriadi
Keyword(s):  
Ethyl Acetate ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Ocimum Basilicum ◽  
Test Method ◽  
Diffusion Method ◽  
Grey Zone ◽  
Root Canals

Pendahuluan: Enterococcus faecalis (E. faecalis) merupakan bakteri yang persisten pada saluran akar gigi, sehingga dapat menyebabkan infeksi sekunder pada saluran akar gigi yang telah diobturasi. Bahan irigasi dan medikamen harus diberikan pada saluran akar gigi untuk menunjang keberhasilan perawatannya. Daun kemangi (Ocimum basilicum/O. basilicum) merupakan tanaman obat tradisional yang diketahui memiliki potensi antibakteri dan berpeluang untuk dikembangkan sebagai bahan irigasi saluran akar gigi. Penelitian ini bertujuan untuk mengetahui zona hambat ekstrak etil asetat daun O. basilicum terhadap E. faecalis ATTC 29212. Metode:Jenis penelitian eksperimental laboratori. Pengujian zona hambat ekstrak etil asetat daun O. basilicum yang diperoleh dari proses maserasi dilakukan dengan metode difusi cakram dengan menggunakan klorheksidin 0,1% sebagai kontrol positif dan dilakukan tiga kali pengulangan terhadap E. faecalis ATCC 29212. Ekstrak pekat dilarutkan menggunakan DMSO 10% dan dilakukan pengenceran dua tingkat sehingga diperoleh konsentrasi ekstrak 512.000 – 1.000 ppm. Hasil: Ekstrak etil asetat daun O. basilicum pada konsentrasi uji 512.000 ppm telah memperlihatkan zona abu-abu seluas 1 mm di luar cakram uji, sementara konsentrasi uji lainnya tidak memperlihatkan adanya penghambatan. Beragam faktor dalam penelitian ini dapat mempengaruhi hasil pengujian, diantaranya adalah kelarutan ekstrak uji dalam pelarut yang digunakan, prosedur sebelum ekstraksi, dan keterbatasan metoda pengujian. Simpulan: Ekstrak etil asetat daun O. basilicum tidak memiliki zona hambat terhadap E. faecalis ATTC 29212.Kata kunci: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zona hambat. ABSTRACTIntroduction: Enterococcus faecalis (E. faecalis) is a bacteria that persist in root canals, cause secondary infection in obturated root canals. Irrigation material and medicament must be administered to the root canal to support the treatment success. Basil leaves (Ocimum basilicum/O. basilicum) is a traditional medicinal plant known with antibacterial potential and has the opportunity to be developed as a root canal irrigation agent. This study was aimed to determine the inhibition zone of the ethyl acetate extract of O. basilicum leaves against E. faecalis ATTC 29212. Methods: This study was an experimental laboratory. The inhibition zone test of the ethyl acetate extract of O. basilicum leaves obtained from the maceration process was conducted with the disc diffusion method using 0.1% chlorhexidine as a positive control and three replications E. faecalis ATCC 29212. The concentrated extract was dissolved using 10% DMSO, and two dilution levels were carried out to obtain an extract concentration of 512,000 - 1,000 ppm. Results: The ethyl acetate extract of O. basilicum leaves at the test concentration of 512,000 ppm showed a grey zone of 1 mm outside the test disc, while other test concentrations did not show any inhibition. Various factors in this study can affect the test results, including the solubility of the test extract in the solvent used, the procedure before extraction, and the limitations of the test method. Conclusion: O. basilicum leaf ethyl acetate extract had no inhibition zone against E. faecalis ATTC 29212.Keywords: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zone of inhibition.

Sign in / Sign up

Export Citation Format

Share Document