scholarly journals Isolasi dan Karakteristik Bakteri Pendegradasi Selulosa dari Limbah Pusat Industri Mebel Antang Makassar

2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Fahruddin Fahruddin
Keyword(s):  
Morphological Characteristics ◽  
Methyl Cellulose ◽  
Colony Structure ◽  
Bacterial Isolates ◽  
Wood Processing ◽  
Gram Staining ◽  
Degrading Bacteria ◽  
Experimental Laboratory ◽  
Microscopic Morphology ◽  
Bacterial Selection

<p>Antang Furniture Center in Makassar is one of the wood industries and is still traditionally. The wood processing produces waste containing cellulose that is difficult to degrade. This study aims to isolate and observe the characteristics of cellulose degrading bacteria. This research was conducted at the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar from March 2019 to November 2019. The method used was an experimental laboratory consisting of <em>Carboxyl Methyl Cellulose</em> (CMC) media for bacterial selection and characterization including microscopic morphology with Gram staining and morphology macroscopic with colony structure. The results showed that cellulose degrading bacteria were characterized by the formation of clear zones formed on CMC media in petri dishes. Based on macroscopic and microscopic morphological characteristics, eleven types of cellulose degrading bacteria isolates were obtained. The isolates obtained can be a source of bacterial isolates for further application in wood waste or other wastes that contain of cellulose.</p>

scholarly journals ISOLASI BAKTERI DARI TANAH TEMPAT PEMBANGAN AKHIR (TPA) AIR SEBAKUL SEBAGAI AGEN BIODEGRADASI LIMBAH PLASTIK POLYETHYLENE

Alotrop ◽  
2020 ◽  
Vol 4 (2) ◽  
pp. 98-106
Author(s):  
Desy Purnama Sari ◽  
Hermansyah Amir ◽  
Rina Elvia
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Isolate ◽  
Morphological Characteristics ◽  
Bacterial Isolation ◽  
Gram Staining ◽  
Chemistry Learning ◽  
Degrading Bacteria ◽  
Identification Of Bacteria ◽  
Time Variations

This study aims to obtain the bacteria of Pseudomonas from the land of TPA Air Sebakul Bengkulu City as a plastic degrading agent and measure the ability of bacteria to degrade Low Density Polyethylene (LDPE) and Oxium plastic. The research was conducted from February to August 2019, at the Laboratory of Biology and Chemistry Learning, Faculty of Teacher Training and Education, University of Bengkulu. Air Sebakul landfill samples were taken at the coordinate point 3o49 '27.8 "S 102o20 '48.4" E. Isolation of plastic degrading bacteria using selective media King's B Agar added with 2% Polyethylen Glycol (PEG) to test the ability to develop bacterial isolates in plastic-based media. The steps of this research are bacterial isolation, bacterial purification and bacterial selection, macroscopic and microscopic identification of bacteria (Gram staining) and plastic biodegradation test with Mineral Salt Agar (MSM) media. Determination of the% weight loss of LDPE and oxium plastics in the biodegradation process was carried out for 30 days with time variations of 10, 20 and 30 days. The results of bacterial isolation based on morphological characteristics and gram staining test of P-1 bacterial isolate have similarities with Pseudomonas aeruginosa. So that the P-1 bacterial isolate is thought to be a Pseudomonas aeruginosa bacterium. The bidoegradation of LDPE and oxium plastics with isolates of P-1 bacteria for 10, 20 and 30 days respectively was able to degrade oxium plastics by 2.43, 5.17 and 9.86% while LDPE plastics by 1.13, 2 and 1 , 17%.

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

scholarly journals Broad diversity of bacteria degrading 17ß-estradiol-3-sulfate isolated from river sediment and biofilm at a wastewater treatment plant discharge

2021 ◽  
Author(s):  
Tamara Mainetti ◽  
Marilena Palmisano ◽  
Fabio Rezzonico ◽  
Blaž Stres ◽  
Susanne Kern ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
River Sediment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Aquatic Environments ◽  
Bacterial Isolates ◽  
Degrading Bacteria ◽  
17Β Estradiol ◽  
Aquatic Wildlife ◽  
Harmful Effects

AbstractConjugated estrogens, such as 17β-estradiol-3-sulfate (E2-3S), can be released into aquatic environments through wastewater treatment plants (WWTP). There, they are microbiologically degraded into free estrogens, which can have harmful effects on aquatic wildlife. Here, the degradation of E2-3S in environmental samples taken upstream, downstream and at the effluent of a WWTP was assessed. Sediment and biofilm samples were enriched for E2-3S-degrading microorganisms, yielding a broad diversity of bacterial isolates, including known and novel degraders of estrogens. Since E2-3S-degrading bacteria were also isolated in the sample upstream of the WWTP, the WWTP does not influence the ability of the microbial community to degrade E2-3S.

Isolation and Characterization of Cellulose Degrading Bacteria of Termite Gut from North Eastern Region of India

2016 ◽  
Vol 5 (6) ◽  
pp. 283-290
Author(s):  
Sonika Sharma ◽  
Soumya Chatterjee ◽  
Sibnarayan Datta ◽  
Rajesh Kumar Prasad ◽  
Angkita Sharma ◽  
...  
Keyword(s):  
Agar Plate ◽  
Bacterial Consortium ◽  
Cellulase Production ◽  
Eastern Region ◽  
Bacterial Isolates ◽  
Carbohydrate Utilization ◽  
North East ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Termite Gut

A study was conducted to screen and isolate cellulase producing bacteria of termite gut from North east region of India. A total of 27 culturable bacterial isolates were screened for cellulase production. Out of the 27 bacterial isolates 11 showed zone of clearance on CMC agar media on staining with 1% Congo red, suggesting potential cellulose degrading activity. The maximum hydrolysis capacities (HC value) on CMC agar plate was found within the range of 3.6 to 40mm. The morphological characterization and gram staining of the positive isolates indicated that 3 isolates were positively stained rods and others were negative cocci. All the cellulase positive isolates were also tested for carbohydrate utilization with maltose, dextrose and fructose, to which all the 11 isolates responded positively. Further, based on the amplification and sequencing of the 16S rRNA genetic region, isolates were identified as member of the genus Bacillus, Paenibacillus and Staphylococcus. The degrading potential of these bacteria were assessed by developing bacterial consortium and efficient degradation was reported after seven days of incubation with different cellulose source like rice, cotton and rice husk.

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

2019 ◽  
Vol 4 (1) ◽  
pp. 79-88
Author(s):  
Evi Octaviany ◽  
Suharjono Suharjono ◽  
Irfan Mustafa
Keyword(s):  
Crude Oil ◽  
16S Rdna ◽  
Bacterial Isolates ◽  
High Concentration ◽  
Isolation And Identification ◽  
Rdna Sequences ◽  
Degrading Bacteria ◽  
16S Rdna Sequences ◽  
Petroleum Contaminated Soil ◽  
Cell Densities

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study. 

scholarly journals Isolation of Polymer-Degrading Bacteria and Characterization of the Hindgut Bacterial Community from the Detritus-Feeding Larvae of Tipula abdominalis (Diptera: Tipulidae)

2007 ◽  
Vol 73 (17) ◽  
pp. 5683-5686 ◽  
Author(s):  
Dana M. Cook ◽  
Emily DeCrescenzo Henriksen ◽  
Rima Upchurch ◽  
Joy B. Doran Peterson
Keyword(s):  
Microbial Community ◽  
Initial Study ◽  
Gene Clone ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Future Studies ◽  
Degrading Bacteria ◽  
Novel Bacteria ◽  
Tipula Abdominalis

ABSTRACT The Tipula abdominalis larval hindgut microbial community presumably facilitates digestion of the lignocellulosic diet. The microbial community was investigated through characterization of bacterial isolates and analysis of 16S rRNA gene clone libraries. This initial study revealed novel bacteria and provides a framework for future studies of this symbiosis.

scholarly journals Isolation, Characterization, and Identification of Bacterial Population from Textile and Tannery Effluents of Bangladesh

2016 ◽  
Vol 29 (2) ◽  
pp. 84-88
Author(s):  
A Hakim ◽  
S Hoque ◽  
SM Ullah
Keyword(s):  
Bacterial Population ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Tannery Effluents ◽  
Bacterial Composition ◽  
Degrading Bacteria ◽  
Diagnostic Characteristics ◽  
Metal Treatment

Ten effluent samples from two different sites located at Hazaribagh tannery belt and Dhaka EPZ, Savar were collected. This study aimed to compare the bacterial composition isolated from tannery and textile effluents and to investigate the occurrence of metal toxicity tolerant and dye degrading bacteria and to select the potential strains for the use in bioremediation. The average bacterial count of HT and DETDE varied in between 3.35×106 and 5.45×106 cfu/mL and 4.8×106 and 7.75×106cfu/mL, respectively. A total of 12 bacterial isolates were characterized as strains of Bacillus, Staphylococcus, and Pseudomonas. A few, however, were re-cultured on other recommended media for verification of diagnostic characteristics. Maximum numbers of bacterial species were isolated from textile effluent. The results showed that a Gram-positive bacillus with a yellow pigment was considered as a major group of the population. Among them three isolates were identified based on alignments of partial sequence of 16S rRNA gene. These are also being used in different wastewater and metal treatment plants all over the world.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 84-88

scholarly journals Isolation and Identification of Novel Microcystin-Degrading Bacteria

2009 ◽  
Vol 75 (21) ◽  
pp. 6924-6928 ◽  
Author(s):  
Pathmalal M. Manage ◽  
Christine Edwards ◽  
Brajesh K. Singh ◽  
Linda A. Lawton
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
First Report ◽  
Degrading Bacteria

ABSTRACT Of 31 freshwater bacterial isolates screened using the Biolog MT2 assay to determine their metabolism of the microcystin LR, 10 were positive. Phylogenetic analysis (16S rRNA) identified them as Arthrobacter spp., Brevibacterium sp., and Rhodococcus sp. This is the first report of microcystin degraders that do not belong to the Proteobacteria.

scholarly journals STUDY OF PREVALENCE OF URINARY TRACT INFECTIONS IN PATIENTS OF UJJAIN, MADHYA PRADESH (INDIA)

2020 ◽  
pp. 69-71
Author(s):  
Kaina Bhonsle ◽  
Harish Vyas ◽  
Kirti Hemwani ◽  
Alka Vyas
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Morphological Characteristics ◽  
Age Group ◽  
Bacterial Isolates ◽  
Gram Stain ◽  
Biochemical Tests ◽  
E Coli ◽  
Tract Infections ◽  
Rural Patients

Background: Urinary tract infections (UTI’s) define a condition in which urinary tract is infected with a pathogen causing inflammation. Urinary tract infections are one of the most prevalent infections affecting people of all age group from neonates to adults and are major cause of morbidity and several disorders in patients. In serious cases urinary tract infection lead to kidney failure, septicemia, bacterial endocarditis, prostatitis and infertility. Aim: The objective of this work was to the study the prevalence of urinary tract infections among patients of Ujjain and to identify prominent bacterial pathogens responsible for causing UTI. Methods: A total of 500 urine samples were collected aseptically and cultured on Blood agar and Mac’conkey agar. The bacterial isolates were identified based on their colony morphological characteristics, Gram stain reaction and their biochemical tests. Result: After overnight incubation a total of 307 out of 500 patients tested positive for UTI suggesting that (61%) of patients suffered from UTI infections. It was seen that (89%) of bacterial isolates causing UTI were Gram negative and (11%) were Gram positive. The identification of pathogens indicated that E. coli is most prevalent uropathogen followed by K. pneumonae, P. aeruginosa, E. faecalis, S. aureus and P.vulgaris. Conclusion: The study shows that prevalence of UTI is higher in females as compared to males. It was also been seen that UTI infections were prevalent in urban as well as in rural patients, however, the incidence of infection was slightly high in urban patients. This study is important as constant survey and identification of uropathogens is essential for effective treatment of UTI.

scholarly journals Isolation and identification of a high-efficiency phenol-degrading bacteria and optimization of its degradation conditions

2021 ◽  
Vol 293 ◽  
pp. 01028
Author(s):  
Haili Sun ◽  
Tianpeng Gao ◽  
Guohua Chang ◽  
Xisheng Tai ◽  
Ruiqi Yang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sodium Chloride ◽  
Low Temperature ◽  
High Efficiency ◽  
Phenol Degradation ◽  
Pcr Amplification ◽  
Toxic Substances ◽  
Isolation And Identification ◽  
Gram Staining ◽  
Degrading Bacteria

Phenol is widely used in China, it not only pollutes the environment, but also accumulates toxic substances in the human body through the food chain, further harming humans. In this experiment, a strain of high-efficiency low-temperature degradation phenol bacteria B5 was selected from the soil contaminated by organic matter of Lanzhou. Through research methods such as Gram staining observation, DNA extraction, PCR amplification, sequencing and comparison, it was found that this strain was Pantoea agglomerans. Through the subsequent optimization of degradation conditions, it was found that the B5 strain can degrade 500mg/L of phenol to 24.8mg/L in 36h. The ability to degrade phenol is stronger between pH5.5-pH6.0, and the ability to degrade phenol is higher in a medium containing 4-8g/L sodium chloride. This research can provide certain theoretical guidance for phenol degradation.

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