scholarly journals Uji Bakteriologis pada Minuman Air Tebu yang Dijual di Pinggiran Jalan Khatib Sulaiman Kota Padang

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Dewi Oktavia Djasmi ◽  
Roslaili Rasyid ◽  
Eliza Anas
Keyword(s):  
Sugar Cane ◽  
Pathogenic Bacteria ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Index Method ◽  
Probable Number ◽  
E Coli ◽  
Cane Juice ◽  
Sugar Cane Juice ◽  
Mpn Test

Abstrak Higinitas penjual dan sanitasi lingkungan yang kurang bersih memungkinkan minuman tebu terkontaminasi oleh bakteri patogen. Lokasi penjualan minuman tebu pinggiran jalan yang terbanyak terletak di jalan Khatib Sulaiman kota Padang. Tujuan penelitian ini adalah menentukan kualitas minuman tebu apakah sesuai dengan persyaratanmikrobiologi.  Penelitian ini bersifat deskriptif dengan menggunakan metode indeks Most Probable Number (MPN) di bagian Mikrobiologi Fakultas Kedokteran Universitas Andalas Padang. Sampel pada penelitian ini adalah seluruh penjual minuman tebu yang ada, yaitu sepuluh minuman tebu yang berasal dari lima pedagang. Sepuluh sampel terdiridari lima minuman tebu yang dicampur es dan lima minuman tebu yang tidak dicampur es. Pemeriksaan MPN yang telah dilakukan terdiri dari dua tes, yaitu: tes presumtif dan tes konfirmatif. Hasilnya adalah seluruh sampel positif mengandung bakteri Coliform dan E. coli. Kesimpulan dari penelitian ini adalah air tebu yang dijual di pinggiran jalan Khatib Sulaiman Padang belum memenuhi standar kelayakan konsumsi secara bakteriologis dan terdapat perbedaan indeks MPN antara minuman tebu yang dicampur es dengan yang tidak dicampur es.Kata kunci: tes MPN, air tebu, hygiene Abstract Pathogenic bacteria can contaminate the sugar cane juice by the less hygiene and environmental sanitation of the sellers. There are a lot of people selling sugar cane juice in Padang, mostly in Khatib Sulaiman. The objective of this study was to determine the quality of the sugar cane juice accordance to microbiological requirements. This descriptive study was using the most probable number (MPN) index method and conducted in Microbiology Laboratory of Medical Faculty, Andalas University Padang.The sample in this study is all of sellers, which is ten cane juices fromfive sellers. Ten samples consists of five sugar cane water with ice and sugar cane water without ice MPN test was done by two tests, the presumptive test and the confirmative test. The result showed that all samples contained coliform bacteria and E.coli. The conclusion of this study is sugarca ne juice which is sold on roadside of Khatib Sulaiman is not worthy to microbiological standards for consumption and there is a difference between the MPN index sugar cane water with ice and sugar cane without ice.Keywords: MPN test, cane juice, hygiene

scholarly journals NILAI MPN (Most Probable Number) DAN DETEKSI BAKTERI Esherichia PADA MINUMAN ES JERUK PERAS di KOTA PONTIANAK

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Ratna Ratna ◽  
Rahmawati Rahmawati ◽  
Mukarlina Mukarlina
Keyword(s):  
Random Sampling ◽  
Pathogenic Bacteria ◽  
Sampling Method ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Normal Limits ◽  
Index Value ◽  
Mpn Test ◽  
Test Result

Iced squeezed orange drinks is one one of processed drinks that made from orange. Good drinking water should not contain pathogenic bacteria or organisms that may harm human health. Coliform is one ofbacteria that may cause disease in humans and being used as indicator bacteria of pathogenic bacteria. This research was aimed to know the MPN value number of coliform bacteria and to identify the bacterial presence of genus Escherichia in iced squeezed orange drinks sold in Pontianak City. The sampling used random sampling method. MPN method was used in order to know the number of coliform in iced squeezed orange drinks in Pontianak City. MPN test result obtained the lowest index value of MPN/mL was 0,14/mL in sample in subdistrict Pontianak City and the highest index value in four subdistricts in Pontianak City was MPN/mL >24,00/mL. It was showed that five samples exceed normal limits in accordance with SNI standards SNI 01-3839-1995 based on Permenkes No 492/MENKES/per/IV/2010. Identification resultshowed the bacterial genus of coliform obtained was member of genus Escherichia.

Analysis of Coliform and Colifecal Total Pollution Test on Various Types of Drinking Water Using the MPN (Most Probable Number) Method

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Wanda Aulya ◽  
Fadhliani Fadhliani ◽  
Vivi Mardina
Keyword(s):  
Drinking Water ◽  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inspection Method ◽  
Sample Number ◽  
Probable Number ◽  
Fecal Coliform Bacteria

Water is the main source for life and also the most severe substance caused by pollution. The mandatory parameters for determining microbiological quality of drinking water are total non-fecal Coliform bacteria and Coliform fecal (Escherichia coli). Coliform bacteria are a group of microorganisms commonly used as indicators, where these bacteria can be a signal to determine whether a water source has been contaminated by bacteria or not, while fecal Coliform bacteria are indicator bacteria polluting pathogenic bacteria originating from human feces and warm-blooded animals (mammals) . The water inspection method in this study uses the MPN (Most Probable Number) method which consists of 3 tests, namely, the presumption test, the affirmation test, and the reinforcement test. The results showed that of 15 drinking water samples 8 samples were tested positive for Coliform bacteria with the highest total bacterial value of sample number 1, 15 (210/100 ml), while 7 other samples were negative. From 8 positive Coliform samples only 1 sample was stated to be negative fecal Coliform bacteria and 7 other samples were positive for Coliform fecal bacteria with the highest total bacterial value of sample number 1 (210/100 ml).

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

Enterobacteriaceae in ground meats

1978 ◽  
Vol 24 (12) ◽  
pp. 1574-1582 ◽  
Author(s):  
Lai-King Ng ◽  
Michael E. Stiles
Keyword(s):  
Ground Beef ◽  
False Positives ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Type I ◽  
Enterobacter Agglomerans ◽  
Frozen Ground ◽  
Probable Number ◽  
E Coli ◽  
Solid Media

Presumptive Escherichia coli counts for 312 samples of non-frozen ground beef were determined and compared with proposed Canadian standards. Results for frozen pork sausages, packaged at manufacturer level, indicated little difference in distribution of presumptive E. coli loads compared with retail ground beef. Use of solid media and direct inoculation of EC broth at 45 °C did not give alternative, rapid methods of estimating E. coli loads in ground beef. Counts on violet red bile agar (VRBA) within 18–24 h incubation at 35 °C gave reliable estimates of coliform bacteria (bile-precipitating colonies) and Enterobacteriaceae (total count), with only 1.3 and 10.7% false positives, respectively. Bile-precipitating isolates from VRBA were primarily E. coli, also Serratia liquefaciens, aerogenic Enterobacter agglomerans, Enterobacter cloacae, Citrobacter freundii, and Klebsiella pneumoniae. Non-bile-precipitating colonies were primarily aerogenic E. agglomerans and S. liquefaciens; however, in the most probable number technique E. agglomerans was screened out. In addition to E. coli, E. agglomerans and S. liquefaciens were the principal types of Enterobacteriaceae in these samples. Enterobacter agglomerans gave a variety of IMViC reactions, including the type I (++−−) reaction, whereas S. liquefaciens were predominantly IMViC type −−++. Incubating EC broth at 45.5 °C, as opposed to 44.5 °C, reduced the number of false positives.

scholarly journals Comparison of the Compact Dry EC with the Most Probable Number Method (AOAC Official Method 966.24) for Enumeration of Escherichia coli and Coliform Bacteria in Raw Meats: Performance-Tested MethodSM 110402

2006 ◽  
Vol 89 (1) ◽  
pp. 100-114 ◽  
Author(s):  
Hidemasa Kodaka ◽  
Shingo Mizuochi ◽  
Hajime Teramura ◽  
Tadanobu Nirazuka ◽  
David Goins ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Soluble ◽  
Test Method ◽  
Gelling Agent ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Official Method ◽  
Probable Number ◽  
E Coli ◽  
Raw Meats

Abstract Compact Dry E. coli/Coliform Count (EC) is a ready-to-use test method for the enumeration of Escherichia coli and coliform bacteria in food. The plates are presterilized and contain culture medium and a cold water-soluble gelling agent. The medium should be rehydrated with 1 mL diluted sample inoculated onto the center of the self-diffusible medium, allowing the solution to diffuse by capillary action. The plate can be incubated at 35C for 2024 h and the colonies counted without any further working steps. The Compact Dry EC medium plates were validated as an analysis tool for determining colony-forming units (CFU) of E. coli and coliform bacteria from a variety of raw meats using 5 different types of raw meats. The performance tests were conducted at 35C. In all studies performed, no apparent differences were observed between the Compact Dry ECmethod and theAOAC Official Method 966.24 results. For the accuracy claim (n = 75), a correlation factor of r2 = 0.93 (E. coli) and r2 = 0.93 (coliform bacteria) could be assigned, as stated in the application for Performance-Tested MethodSM.

scholarly journals Microbial contamination including Vibrio cholerae in fishery auction markets in West Sea, South Korea

2019 ◽  
Vol 22 (1) ◽  
Author(s):  
Yukyung Choi ◽  
Yewon Lee ◽  
Soomin Lee ◽  
Sejeong Kim ◽  
Jeeyeon Lee ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Environmental Samples ◽  
Sea Bass ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Pcr Analysis ◽  
Probable Number ◽  
Auction Markets ◽  
E Coli ◽  
Fishery Products

Abstract Background The monitoring of pathogens of fishery auction markets is important to obtain safe fishery products regarding hygiene and sanitation. In this study, aerobic, coliform, Escherichia coli, and Vibrio cholerae were monitored in the fishery products and environmental samples obtained from fishery auction markets. Methods The fishery products (flounder, octopus, skate, rock cod, sea bass, snail, monkfish, flatfish, comb pen shell, corb shell, conger eel, hairtail, croaker, and pilchard) were placed in filter bags, and the environmental samples (samples from the water tanks at the fishery auction markets, seawater from the fishery distribution vehicles, ice from wooden or plastic boxes, and surface samples from wooden and plastic boxes used for fish storage) were collected. Aerobic bacteria, E. coli, and coliform in the samples were enumerated on aerobic count plates and E. coli/coliform count plates, respectively. For V. cholerae O1 and V. cholerae non-O1 quantification, most probable number (MPN)-PCR analysis was performed. Results Aerobic and coliform bacteria were detected in most samples, but E. coli was not detected. Wooden boxes were contaminated with high levels of aerobic and coliform bacteria in all seasons (spring, summer, and fall). During fall, V. cholerae non-O1 were detected in snails, hairtails, croakers, flatfishes, pilchards, plastic boxes, and water samples. Conclusions These results indicate an increased prevalence of V. cholerae contamination in fishery products in fall, including food contact samples, which can be vehicles for cross-contamination.

Comparison of the SimPlate Coliform and Escherichia coli Test with Petrifilm, Three-Tube MPN, and VRBA + MUG Methods for Enumerating Coliforms and E. coli in Food

1998 ◽  
Vol 61 (4) ◽  
pp. 444-449 ◽  
Author(s):  
D. E. TOWNSEND ◽  
R. L. IRVING ◽  
A. NAQUI
Keyword(s):  
Escherichia Coli ◽  
Correlation Coefficients ◽  
Raw Milk ◽  
Food Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Additional Food ◽  
Suitable Alternative ◽  
Probable Number ◽  
E Coli

SimPlate for coliforms and Escherichia coli (CEc) is a new method for the detection and quantification of coliforms and E. coli in food. Internal validation of the method was carried out at IDEXX Laboratories (Westbrook, ME) with 180 food samples representing a variety of different food matrices and compared against three-tube MPN (most probable number), VRBA (violet red bile agar) + MUG, and Petrifilm (E. coli count) methods. SimPlate CEc was highly correlated with each of these methods for the quantification of coliform bacteria (r ≥ 0.90). An insignificant number of food samples were found to contain E. coli; therefore, no meaningful correlation data could be generated. Four hundred forty-four additional food samples were tested at five collaborating laboratories for the presence of coliforms and E. coli using SimPlate CEc and either VRBA + MUG or Petrifilm (E. coli count). Regression analysis of data from SimPlate for CEc versus Petrifilm E. coli count plates generated correlation coefficients (r) of at least 0.89 for total coliforms and at least 0.90 for generic E. coli. Correlation coefficients between SimPlate for CEc and VRBA + MUG data were at least 0.90 for coliforms and at least 0.86 for E. coli. SimPlate for CEc demonstrated better recovery of E. coli than Petrifilm when high populations of bacteria were present. E. coli was not detected in 20 of 50 (40%) raw milk samples tested by the Petrifilm method due to the presence of interfering coliform and noncoliform bacteria. It is concluded that SimPlate for CEc is a suitable alternative for determining numbers of coliform bacteria and E. coli in food.

scholarly journals Kualitas Air Sumur Gali Kelurahan Lubuk Buaya Kecamatan Koto Tangah Kota Padang Berdasarkan Indeks Most Probable Number (MPN)

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Randa Novalino ◽  
Netti Suharti ◽  
Arni Amir
Keyword(s):  
Water Quality ◽  
Health Department ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Well Water ◽  
Health Minister ◽  
Probable Number ◽  
Sources Of Pollution ◽  
Dug Well ◽  
Mpn Test

AbstrakDiare merupakan salah satu penyakit yang ditularkan melalui air terkontaminasi oleh agen penyebab seperti bakteri Coliform.  Menurut data Dinas Kesehatan Kota (DKK) Padang pada tahun 2011, kejadian diare di Kelurahan Lubuk Buaya Kecamatan Koto Tangah, Kota Padang merupakan kasus tertinggi di Kota Padang. Tujuan penelitian ini adalah menentukan kualitas air sumur gali di Kelurahan Lubuk Buaya berdasarkan Indeks Most Probable Number (MPN) menurut Peraturan Menteri Kesehatan Republik Indonesia (Permenkes RI) No. 416 tahun 1990. Sampel penelitian ini adalah  air sumur gali yang digunakan di beberapa Rukun Tetangga (RT), yang diambil secara acak dari beberapa Rukun Warga (RW) yang telah dipilih sebelumnya, sehingga didapatkan 15 sampel. Penelitian ini dilaksanakan dalam 2 tahap yaitu pengambilan sampel air sumur gali sekaligus observasi faktor yang mempengaruhi kualitas air dan pemeriksaan mikrobiologi dengan metode Most Probable Number (MPN) Test. Tes ini terdiri dari tes presumtif dan tes konfirmatif yang disesuaikan dengan Permenkes RI. Hasil penelitian ialah 73,33% dari jumlah sumur yang diperiksa tidak memenuhi standar Permenkes R.I. karena mengandung Coliform > 50 pada setiap 100 ml air. Hanya 26,6% sumur yang memenuhi standar yang telah ditetapkan. Beberapa faktor yang dapat mempengaruhi yaitu lokasi sumber pencemaran, dinding parapet, drainase, tutup sumur dan sarana pengambilan air.Kata kunci: kualitas air sumur gali, MPN, coliform AbstractDiarrhea is one of the diseases that  transmitted through contaminated water by causative agent, one of which is coliform bacteria. According to data from City Health Department Padang in 2011, the incidence of diarrhea in Kelurahan Lubuk Buaya Kecamatan Koto Tangah – Padang is the highest case in the city of Padang. The objective of this study was to determine the water quality of wells dug in Kelurahan Lubuk Buaya by Most Probable Number Index (MPN) according regulation of Indonesian health minister. The 15 samples was water of dug well  in some of the RT households from several neighborhoods that was selected. The research was conducted in two stages, dug well water samples as well as observation of the factors that affect water quality and microbiological examination of the Most Probable Number method (MPN) Test. This test consists of presumptive tests and confirmative tests that were tailored to regulation of Indonesian health minister.  The result were 73.33% of the wells tested did not meet the standards of Indonesian health minister regulation, because it contains >50 coliform in every 100 ml of water. Only 26.6% of the wells were inspected to meet the standards set. Several factors can affect the location of sources of pollution, parapet walls, drainage or sewer water, cover the wells, and water collection facilities.Keywords: dug well water quality, MPN, coliform

Incidence and Behavior of Salmonella and Escherichia coli on Whole and Sliced Zucchini Squash (Cucurbita pepo) Fruit

2010 ◽  
Vol 73 (8) ◽  
pp. 1423-1429 ◽  
Author(s):  
JAVIER CASTRO-ROSAS ◽  
EVA MARÍA SANTOS LÓPEZ ◽  
CARLOS ALBERTO GÓMEZ-ALDAPA ◽  
CESAR ABELARDO GONZÁLEZ RAMÍREZ ◽  
JOSÉ ROBERTO VILLAGOMEZ-IBARRA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Inoculum Level ◽  
Probable Number ◽  
Initial Inoculum ◽  
E Coli ◽  
Thermotolerant Coliforms ◽  
Salmonella Serotypes ◽  
And Behavior

The incidence of coliform bacteria (CB), thermotolerant coliforms (TC), Escherichia coli, and Salmonella was determined for zucchini squash fruit. In addition, the behavior of four serotypes of Salmonella and a cocktail of three E. coli strains on whole and sliced zucchini squash at 25 ± 2°C and 3 to 5°C was tested. Squash fruit was collected in the markets of Pachuca city, Hidalgo State, Mexico. CB, TC, E. coli, and Salmonella were detected in 100, 70, 62, and 10% of the produce, respectively. The concentration ranged from 3.8 to 7.4 log CFU per sample for CB, and <3 to 1,100 most probable number per sample for TC and E. coli. On whole fruit stored at 25 ± 2°C or 3 to 5°C, no growth was observed for any of the tested microorganisms or cocktails thereof. After 15 days at 25 ± 2°C, the tested Salmonella serotypes had decreased from an initial inoculum level of 7 log CFU to <1 log, and at 3 to 5°C they decreased to approximately 2 log. Survival of E. coli was significantly greater than for the Salmonella strains at the same times and temperatures; after 15 days, at 25 ± 2°C E. coli cocktail strains had decreased to 3.4 log CFU per fruit and at 3 to 5°C they decreased to 3.6 log CFU per fruit. Both the Salmonella serotypes and E. coli strains grew when inoculated onto sliced squash: after 24 h at 25 ± 2°C, both bacteria had grown to approximately 6.5 log CFU per slice. At 3 to 5°C, the bacterial growth was inhibited. The squash may be an important factor contributing to the endemicity of Salmonella in Mexico.

ColiComplete® Substrate-Supporting Disc Method for Confirmed Detection of Total Coliforms and Escherichia coli in All Foods: Comparative Study

1994 ◽  
Vol 77 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Philip T Feldsine ◽  
Maria T Falbo-Nelson ◽  
David L Hustead
Keyword(s):  
Escherichia Coli ◽  
Comparative Study ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Total Coliforms ◽  
E Coli ◽  
Total Coliform Bacteria ◽  
Better Than

Abstract The ColiComplete® substrate-supporting disc (SSD) method for simultaneous confirmed total coliform count and Escherichia coli determination in all foods was compared with the AOAC most probable number (MPN) methods 966.23 and 966.24. In this comparative study, 20 water and food types were analyzed; 7 of these foods were naturally contaminated with coliform bacteria, 6 food types were naturally contaminated with E. coli, and the remaining foods were inoculated with coliform bacteria and/or E. coli. Data were analyzed separately for total coliform bacteria and for E. coli. Mean log MPN counts were determined by the SSD method and the appropriate AOAC MPN procedure. Results were then analyzed for mean log MPN differences and variance, according to methods described by AOAC INTERNATIONAL Results for both total conforms and E. coli indicate that the SSD method is equivalent to or better than AOAC MPN methods 966.23 and 966.24.

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