scholarly journals Pengaruh Variasi pH Saliva terhadap Perlekatan Streptococcus mutans pada Resin Komposit Nanofil

2017 ◽  
Vol 6 (2) ◽  
pp. 51
Author(s):  
Rahma Syarafina Maharani ◽  
Widowati Siswomiharjdo ◽  
Siti Sunarintyas

Nanofilled composite resin restorations are in contact with saliva.Salivary pH affect the hydrolysis process and produce a surface roughness.S.mutansadhesion occur in a rough area of the oral cavity.The aim was to determine the effect of variations in salivary pHonS.mutansadhesion to nanofilled composite resin.Nanofilled composite resin(Filtex Z350XT-3M ESPE)5mm diameter and 2mm thick(N=12)were divided into three groups(n=4)and immersed in 5ml artificial saliva pH 3.5, pH 7, pH 8, incubated 14 days,37°C. Samples were soaked into saliva 1 hour,37 C. Samples were put into bacterial suspension 24 hours,37°C, diluted to 10.0.1ml of the final dilution were cultured on BHI agar, incubated 48 hours,37°C. S.mutans colonies were determined using Colony Counter(CFU/ml). Data were analyzed with One-Way ANOVA test(a=0.05) and LSD0.05. The test showed there is influence ofthe variations  in salivary pHonS.mutans adhesionto nanofilled composite  resin(p<0.05). Based on LSD0.05 test, there are significant differences between the pH 3.5 group and the pH 7; pH 8 groups, butnot between the pH 7group and the pH 8 group. Variations in salivary pH affectedS.mutansadhesion to nanofilled composite resin.The acidic pH of saliva increased the S.mutansadhesion on nanofilled composite resin compared with the neutral or alkaline.

2017 ◽  
Vol 6 (2) ◽  
pp. 25
Author(s):  
Aufia Espressivo ◽  
Dyah Irnawati ◽  
Purwanto Agustiono

Nanohybrid composite resin is a type of composite resin restorative material containing micro-sized filler particles incorporated with nanoparticles. Composite resin restoration in the oral cavity can release its filler particles, one of them is aluminum ion. The pH of saliva is varied. This research aimed to determine the influence of salivary pH on aluminum ion solubility of nanohybrid composite resin. The materials used were nanohybrid composite resin Tetric Evoceram (Ivoclar Vivadent, Austria) and artificial saliva with pH of 5.6, 6.7, and 8.0. Twelve disc-shaped composite resin samples sized 5 mm in diameter and 2 mm thick were made. Samples were polymerized using light curing unit for 20 seconds. Composite resin samples were divided into three pH groups (n=4) and then each sample was soaked in 10 mL artificial saliva inside of polyethylene bottle. The immersion was done in the incubator for 14 days at 37ºC. The concentration of aluminum ion in artificial saliva solutions subsequently was calculated using AAS. Data were analyzed using One-way ANOVA (a=0,05). The mean concentration of aluminum ion released into saliva with pH 5.6, 6.7, and 8.0 were 0.538 ± 0.996 ppm, 0.431 ± 0.981 ppm, and 0.350 ± 0.071 ppm respectively. The result of One-way ANOVA showed a significant influence of salivary pH variation to the solubility of aluminum ion on nanohybrid composite resin (p<0,05). LSD test showed a significant difference only between saliva pH 5,6 and 8,0. The conclusion of this research was that low salivary pH increased the solubility of aluminum ion on nanohybrid resin composite in saliva.


2017 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Zwista Yulia Dewi ◽  
Widjijono Widjijono ◽  
Erna Prawita Setyowati

The material in the oral cavity will be coated by saliva. The humidity of material that is immersed  in water will increase from time to time. Longer immersion  time  in water causes  an increase in the absorption of water by a material. Non-dental glass fiber can be used as an alternative replacement for fiber glass dental. The advantages of dental glass fiberis widely available with  reasonably  priced. Fiber glass  non dental commonly used as building materials and automotive. Streptococcus mutans bacteria have the ability to stick to the whole surface of the mouth, including dental materials. The purpose of this study is to determine the effect of dental glass fiber and non-dental glass fiber immersion in artificial saliva  for 14 days to the adhesions of Streptococcus mutans bacteria.Fiber used in this study is E-glass fiber dental(Fibersplint, Polydentia SA, Switzerland), non-dental glass fiber A (LT, China), flowable composite (CharmFil Flow, Denkist, Korea) and silane coupling agent (Monobond S, Ivoclar Vivadent, Liechtenstein). Subjects were divided into three groups, each group consisting of 5 samples. Streptococcus mutans bacteria  was planted in BHI media and calculated by  plate count method. Results were analyzed using ANAVA. From the research it showed that the average dental glass fiber bacterial adhesions value was (953.4 ± 7.19), non-dental glass fiber(953.4 ± 7.19) while the composite resin without using fiber glass (848 ± 7.07). Statistical analysis showed a variable composition have a significant effect (p <0.05). The conclusion of this research that the alkali metal content in the fiber glass can affect the amount of bacteria Streptococcus mutans adhesions.


e-GIGI ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Fransiska M. Pardosi ◽  
Darmawati A. Indraswari ◽  
Lusiana Batubara ◽  
Nadia Hardini

Abstract: Nanofiller composite resin has small filler size which enhances its properties. However, these properties could decrease due to several factors. Acidic liquid such as coffee could reduce the hardness of composite resin. This study was aimed to analyze the effect of robusta and arabica coffee immersion on the hardness of nanofiller composite resin. A total of 27 composite resin samples were used and then were polished by using a soflex disc for 30 seconds on each roughness level. Samples were then divided into three groups, as follows: the control group with artificial saliva and the treatment groups with robusta coffee and arabica coffee. The composite resin molds were immersed in the three groups for 5 days. After five days of immersion, the hardness of the sample was tested by using a Vickers hardness tester. Artificial saliva as the control group had the highest mean hardness value of 112.98±8.67 VHN, followed by robusta coffee, and then by arabica coffee. The One Way Anova and post hoc LSD test showed that there were significant differences in all groups (p<0.05) except for the two treatment groups, namely robusta and arabica coffee groups which did not show any significant difference in the resin hardness (p>0.05). In conclusion, robusta and arabica coffee affect the hardness of the nanofiller composite resin, but there is no significant difference in the hardness between the robusta and arabica coffee groups.Keywords: composite resin hardness, nanofiller, robusta coffee, arabica coffee Abstrak: Resin komposit nanofiller memiliki ukuran filler kecil yang dapat meningkatkan fungsinya namun fungsi tersebut dapat menurun oleh beberapa hal. Cairan asam yang dikonsumsi seperti kopi dapat menurunkan kekerasan resin komposit. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman kopi robusta dan arabika terhadap kekerasan resin komposit nanofiller. Sampel resin komposit yang digunakan sebanyak 27 buah dan dilanjutkan dengan pemolesan menggunakan soflex disc selama 30 detik setiap tingkat kekasarannya. Sampel kemudian dibagi dalam tiga kelompok, yaitu kelompok kontrol dengan saliva buatan dan dua kelompok perlakuan dengan kopi robusta dan arabika. Sebanyak 27 buah resin komposit direndam ke dalam tiga kelompok tersebut selama lima hari kemudian diuji kekerasannya dengan vickers hardness tester. Saliva buatan sebagai kelompok kontrol memiliki nilai rerata kekerasan tertinggi sebesar 112,98±8,67 VHN, diikuti kopi robusta, dan nilai terendah yaitu kopi arabika. Hasil uji One Way Anova dan post hoc LSD menunjukkan terdapat perbedaan bermakna pada semua kelompok (p<0,05) kecuali pada kedua kelompok perlakuan yaitu antara kopi robusta dan arabika tidak menunjukkan adanya perbedaan kekerasan yang bermakna (p>0,05). Simpulan penelitian ini ialah kopi robusta dan arabika memengaruhi kekerasan resin komposit nanofiller namun tidak terdapat perbedaan kekerasan antara keduanya.Kata kunci: kekerasan resin komposit, nanofiller, kopi robusta, kopi arabika


2015 ◽  
Vol 20 (2) ◽  
pp. 156
Author(s):  
Prasetyo Adi ◽  
Ambar Puspitasari ◽  
Murni Ukhuwah I

Saliva adalah sekelompok cairan oral yang kompleks. Salah satu mikroorganisme yang menyebabkan pH saliva menjadi asam adalah Streptococcus mutans. Rebusan kelopak bunga Rossella (Habiscus sabdariffa L) mengandung flavonoid dan antosianin yang dapat mengganggu aktivitas sel dan pertumbuhan Streptococcus mutans. Tujuan penelitian ini mengetahui pengaruh konsentrasi rebusan kelopak bunga Rossella terhadap pH dan absorbansi saliva buatan yang diinduksi Streptococcus mutans secara in vitro. Penelitian ini menggunakan True Experimental Design yaitu Posttest Control Group Design. Konsentrasi yang digunakan dalam penelitian ini adalah 5%, 15%, 25%, dan 35%. Hasil penelitian menunjukkan ada perbedaan nilai pH dan absorbansi saliva buatan yang telah diinduksi Streptococcus mutans dan ditambahkan dengan rebusan kelopak bunga Rossella terhadap kelompok kontrol secara in vitro. Analisa data menggunakan uji Korelasi dan Regresi menunjukkan pengaruh sebesar 99,2% pada pemberian rebusan kelopak bunga Rossella terhadap nilai pH dan absorbansi saliva buatan. Uji One-Way ANOVA menunjukkan nilai signifikansi 0,000 (p< 0,05). Disimpulkan terdapat perbedaan nilai pH dan absorbansi saliva buatan yang signifikan antara kelompok kontrol dan kelompok perlakuan.The Effect of Rossella Flower Petals Stew to Artificial Saliva pH. Saliva is a complex oral fluids. One of the microorganisms that cause the saliva’s pH becomes acid is Streptococcus mutans. Water stew of Rossella flower petals (Habiscus sabdariffa L) contains flavonoids and anthocyanins that interfere metabolism of the cells and growth of Streptococcus mutans. The purpose of this experimental was to know the effect of water stew Rossella flower petals concentration in pH scale and absorbance score of artificial saliva which induced with Streptococcus mutans by in vitro. The method which applied here was True Experimental Design, post test control group design. The concentrations that used in this study were 5%, 15%, 25% and 35%. The results described the differences in pH scale and absorbance score of artificial saliva which induced with Streptococcus mutans between treatment group (added by water stew Rossella petals) and control group. Data analysis using correlation and regression test showed the effect of water stew of Rossella flower petals in pH scale of artificial saliva was 99.2%. The conclusion of this experimental explained the increase of pH scale after added with the water stew of Rossella flower petals, also showed a significant differences of pH scale of artificial saliva between treatment group and control group based on the One-Way ANOVA test, and the water stew of Rossella flower petals was effective to maintain pH scale of artificial saliva approach normal pH scale


2019 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Andi Kurniawan ◽  
Ketut Suardita ◽  
Nanik Zubaidah

Back Ground: Adherence of  Stretococcus mutans biofilm (S. mutans) to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. There are two type of nanofil composite: universal restorative and flowable restorative. That have different characteristic to induce S.mutans biofilm adherent in it surface.  Purpose: The aim of this study was to compare the adherence of S. mutans biofilm to two types of nanofil restorative materials, flowable restorative and universal restorative.. Materials and Methode: 32 disc-shaped specimens (∅ = 5.0 mm / thickness = 2.0 mm) of two types  composite were divided  to 4 groups (n = 8): group 1; universal restorative were immersed in pH cycling solution for 14 days ,group 2; Universal restorative  were  immersed in water for 14 day,group 3: flowable restorative were immersed in pH cycling solution,group 4: flowable restorative were immersed  for 14 days in water .  in day 15 th,  All speciments(n=32) were immersed for 24 hours in artificial saliva.. Streptococcus mutans cells were brought in contact with and grown on the speciments for 48 hours in BHI-B. Bacterial suspension was deposited onto each material and the adhesion of biofilm was evaluated trough optic density (OD) . Optic density   biofilm of S.mutans analyzed using Elissa reader’ Spectrophotometry. Statistical analysis was performed by Kruskall -wallis and Tukey HSD test  (α = 0.05). Result: Adherence of  S.mutans biofilm on flowable restorative (mean OD:1,933, SD: 0,633)  were significantly higher than universal restorative materials (mean OD: 1,240,SD:0,317). (P<0,05) Conclusion: The adherence of S.mutans biofilm on the surface of composites resin nanofil flowable restorative higher than universal restorative.


2021 ◽  
Vol 22 (3) ◽  
pp. 179-184
Author(s):  
Carlos Frederico Bettcher Silva ◽  
Gabriel Batista de Souza ◽  
Luan Miranda Mori ◽  
Ricardo Huver de Jesus ◽  
Jefferson David Melo de Matos ◽  
...  

AbstractThe choice of the best finishing/polishing methods for composite resin restorations is critical to the choice of excellence results related to longer clinical longevity. The objective of the study was to evaluate the influence of finishing and polishing systems on the surface roughness of a Nanoparticulate and Bulk-fill resin. For the research, 40 sample disks were prepared for each composite: single-increment (BF) (Bulk Fill 3M), both 2 mm thick and 10 mm in diameter (FZ) (Filtek Z350, 3M). The specimens were randomly divided into 4 groups with 10 samples each, according to the different materials to which they were subjected to the finishing and polishing protocols: control [C]- diamond tip (FG 4137F, FAVA); [EN] polishing discs (Enhance, Dentsply); [SL] Abrasive discs (Sof-Lex Pop-on, 3M) and [PD] felt disc + Polishing paste (Diamond Excel, FGM). A surface roughness (Ra) was verified with a roughness meter in three different data volumes and calculated as arithmetic means. Data were statistically treated by one way ANOVA followed by test t and student t test. Regardless of the data tested, evidence of the systemic controlling against different systems for polishing control with control (p <0.05). PD presented statistically greater roughness to the EN and SL (p <0.001), these in turn obtained similar performance and presented the lowest values of roughness for both composites (p> 0.05). The different finishing / polishing methods influenced the surface roughness of both composites, observing for Sof-lex and Enhance lower Ra values.Keywords: Nanoparticles. Dental Polishing. Composite Resins.ResumoA escolha dos adequados métodos de acabamento/polimento para restaurações de resina composta é fundamental para obtenção de resultados de excelência, relacionados a maior longevidade clínica. O objetivo desse estudo foi avaliar a influência dos sistemas de acabamento e polimento na rugosidade de superfície das resinas nanoparticuladas convencional e bulk-fill. Para a pesquisa foram confeccionados 40 discos de amostra para cada compósito: Convencional [FZ] (Filtek Z350, 3M) e de incremento único [BF] (Filtek Bulk Fill, 3M), ambas com 2 mm de espessura e 10 mm de diâmetro. Foram divididos aleatoriamente em 4 grupos com 10 amostras cada, de acordo com os diferentes protocolos que receberam, sendo: controle (C) ponta diamantada (FG 4137F, FAVA); (EN) discos de silicone (Enhance, Dentsply); (SL) discos abrasivos (Sof-Lex Pop-on, 3M); (PD) Feltro + Pasta diamantada (Diamond Excel, FGM). A rugosidade de superfície (Ra) foi verificada com um rugosímetro em três leituras de diferentes direções e calculadas as médias aritméticas. Os dados foram tratados estatisticamente pelos testes ANOVA 1 fator seguido pelo teste t e t de student (p<0,05). Independente da resina testada, evidenciou-se diminuição da rugosidade pelos diferentes sistemas de polimento frente ao grupo controle (p<0,05). PD apresentou rugosidade estatisticamente maior à EN e SL (p<0,001), estes últimos que por sua vez obtiveram desempenho semelhante e apresentaram os menores valores de rugosidade para ambos os compósitos (p>0,05). Os diferentes métodos de acabamento/polimento influenciaram na rugosidade de superfície dos dois compósitos testados, observando para Sof-lex e Enhance menores valores de Ra. Palavras-chave: Nanopartículas. Polimento Dentário. Resinas Compostas.


2017 ◽  
Vol 50 (4) ◽  
pp. 178
Author(s):  
Tunjung Nugraheni ◽  
N Nuryono ◽  
Siti Sunarintyas ◽  
Ema Mulyawati

Background: Restoration of the teeth immediately after bleaching with 35% hydrogen peroxide (H2O2) is contraindicated due to the remnant of free radicals that will stay inside enamel and dentin for 1-3 weeks and reduce the adhesion of composite resin. Sodium ascorbate is an antioxidant substance known to bind free radical residues, thereby shortening the delay in restoration. Purpose: The purpose of this study was to examine the resin bond strength of bleached dentin influenced by the application of 35% sodium ascorbate. Methods: Nine premolars were divided into their crown and root sections, with the crown subsequently being cut into four equal parts to obtain 36 samples. These were then divided into four groups, each containing nine samples. Group A (control): samples were bleached using35% hydrogen peroxide, immersed in an artificial saliva, stored in an incubator at 37°C for seven days and then filled with a composite resin. Group B:samples were also bleached by means of 35% H2O2 followed by one application of 0.025 ml 35% sodium ascorbate for 5 minutes and restored with composite resin. Group C: samples were bleached with 35% H2O2, followed by two applications of 0.025 ml 35% sodium ascorbate for 5 minutes, and restored with a composite resin. Group D: dentin was bleached with 35% H2O2 followed by three applications of 0.025 ml sodium ascorbate 35% for 5 minutes and restored with a composite resin. The shear bond strength of the composite resin was measured by a universal testing instrument (Zwick, USA). Data was analyzed by means of one-way Anova and LSD. Results: The highest mean shear bond strength of composite resin was in group C, while the lowest was in group B. The result of one-way Anova indicated a difference in the shear bond strength of composite resin in the four treatment groups (p < 0.05). An LSD test showed there to be a difference in shear bond strength of composite resin between group A and groups C and D or between group B and groups C and D. There was no difference in shear bond strength of composite resin between group A and group B or between group C and group D. Conclusion: Application frequency of 35% sodium ascorbate affect on shear bond strength of composite resin restoration in bleached dentin by 35% H2O2.


2017 ◽  
Vol 8 (4) ◽  
pp. 288-295
Author(s):  
Sahand Rikhtegaran ◽  
Mahdi Rahbar ◽  
Narmin Mohammadi ◽  
Shadieh Mowlaie ◽  
Siavash Savadi-Oskoee ◽  
...  

ABSTRACT Introduction Adhesion of bacteria, especially Streptococcus mutans (S. mutans), to the surface of tooth restorations is a factor in the etiology of secondary caries. Given the ever-increasing popularity of bleaching procedures, the aim of the present study was to evaluate adhesion of S. mutans and surface roughness (SR) of microhybrid composite resin and giomer subsequent to the application of 15% carbamide peroxide. Materials and methods Twenty disk-shaped samples were prepared from each material, measuring 8 mm in diameter and 2 mm in thickness. Then, the samples of each material were divided into two groups (n = 10): (a) microhybrid without bleaching; (b) microhybrid with bleaching; and (c) giomer without bleaching; and (d) giomer with bleaching. The samples in groups I and III were immersed in artificial saliva for 14 days without any bleaching procedure; the samples in groups II and IV underwent a bleaching procedure on their polished surfaces with 15% carbamide peroxide for 14 days (4 hours of bleaching and 20 hours of immersion in artificial saliva). The SR of all the samples was determined with the use of a profilometer. The samples were added to the culture medium after 4 hours of placement in a microbial suspension at 37°C; after 24 hours of incubation at 37°C, the bacterial counts, indicating the number of bacteria adhering to the surface, were determined by counting them in the plates containing the solid culture medium. Results The type of the restorative material had a significant effect on SR, with greater SR in giomer (p = 0.03). However, bleaching had no significant effect on SR (p = 0.099). In relation to the rate of bacterial adhesion (BA), both the types of the restorative materials and bleaching procedures were significantly effective; in this context, there was more BA in microhybrid composite resin samples that did not undergo bleaching (p < 0.001). Bleaching resulted in the adhesion of S. mutans to the surface of both materials decrease. Pearson's correlation coefficient did not reveal any correlation between BA and SR (p = 0.42). Conclusion The BA was higher in microhybrid composite resin, and SR was higher in giomer. The BA was higher in samples that did not undergo a bleaching procedure. Clinical significance There is no change in the SR of microhybrid composite resin and giomer after application of 15% carbamide peroxide; therefore, it is not necessary to polish or replace these restorative materials after bleaching. In addition, use of 15% carbamide peroxide does not increase caries risk. How to cite this article Mohammadi N, Mowlaie S, Savadi- Oskoee S, Ebrahimi ME, Rikhtegaran S, Rahbar M, Pirzadeh T. Effect of 15% Carbamide Peroxide on the Surface Roughness and Adhesion of Streptococcus mutans to Microhybrid Composite Resin and Giomer. World J Dent 2017;8(4):288-295.


2015 ◽  
Vol 16 (10) ◽  
pp. 800-804
Author(s):  
Mário Alexandre Coelho Sinhoreti ◽  
Marcos Aurélio Bonfim da Silva ◽  
Rafael Pino Vitti ◽  
Rafael Leonardo Xediek Consani ◽  
José Ginaldo da Silva ◽  
...  

ABSTRACT Aim The purpose of this study was to determine the effect of different solutions cola soft drink (CSD) and coffee on roughness and microleakage of composite resin restorations. Materials and methods Sixty bovine incisors were prepared and restored using Filtek Z350 XT (3M/ESPE) nanoparticulate resin. The samples were divided into three groups (n = 20): A (control)-Immersion in artificial saliva (14 days); B: Immersion in coffee (14 days), for 15 minutes (3x/day) and C: Immersion in CSD for 14 days (3x/day). Then the samples were analyzed for microleakage (dye penetration) and surface roughness (atomic force microscope). The one-way analysis of variance (ANOVA) was applied to assess the surface roughness and microleakage. The Tukey's test was set at 0.05. Results Group A (roughness-GAR) presented significantly the lowest average surface roughness. Group C (microleakage- GCM) showed significantly the highest average microleakage. Conclusion It was concluded that CSD and coffee change the surface roughness and increase the microleakage of restorations. Clinical significance Nowadays there is a high consumption of artificially sweetened soft drinks, sports drinks, high-energy beverages and coffee products by people that cause problems in composite resin restorations. How to cite this article da Silva MAB, Vitti RP, Sinhoreti MAC, Consani RLX, da Silva JGJ, Tonholo J. Evaluation of the Surface Roughness and Microleakage of Dental Composites Exposed to Different Beverages. J Contemp Dent Pract 2015;16(10):800-804.


Author(s):  
Nurcan Ozakar Ilday ◽  
Ozcan Karatas ◽  
Latife Altınok Uygun ◽  
Pınar Gul

Objective: The objective of this in vitro study is to examine the effect of antioxidants on the bonding strength of composite resin to bleached enamel. Materials and Methods: One hundred twenty non decay teeth were used in this study. Specimens were cut from the enamel cement junction. Then the specimens were randomly assigned into two groups-office bleaching and home bleaching. Bleaching antioxidants were applied to the first 12 subgroups. The specimens in the remaining 12 groups were kept in artificial saliva for 14 days and antioxidants were applied. Composite resin restoration was applied to all specimens. A cutting device was employed to obtain six specimens (0.9mm) from each tooth, and the microtensile bond strength (μTBS) test was performed. Data were analyzed using one-way ANOVA, the paired t-test, and Duncan’s post hoc test (p=0.05). Results: In office bleaching specimens, immediate antioxidant application increased the μTBS values (p<0.05). Comparisons of the antioxidants individually revealed that bonding values increased after two weeks in specimens subjected to antioxidant application (p<0.05). In office bleaching specimens, immediate antioxidant application increased the μTBS values (p>0.05). Mean μTBS values in the groups to which antioxidants were applied two weeks after home bleaching were higher than those in the groups in which antioxidants were applied immediately. Conclusion: The use of antioxidants after bleaching may increase the MTBS value.  The clinician should take this into account especially in immediate restoration applications after bleaching.


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