Reproductive evaluation of bucks (Capra hircus L.) with usual management  in herds from Benito Juarez, Guerrero, Mexico

Objective: To evaluate the reproductive characteristics of male goats (Capra hircus L.)with habitual management on their herds at Benito Juarez municipality, Guerrero, Mexico.Design / methodology / approach: Ten male Creole goats from seven herds wereevaluated, aged between 2 to 7 years and 2 to 3 body condition (BC). The males wereevaluated regarding their sexual behavior, sperm quality, physical examination andreproductive clinical evaluation.Results: All the assessed male Creole goats displayed sexual behavior (P < 0.05).However, for the males of three of the evaluated herds the sexual behavior was lower (P < 0.05). Of the ten electro-ejaculated males, 20% ejaculated and 80% did not (P < 0.05). From the males that ejaculated, a sperm concentration of 224 x 106 and 16 x 106 spermmL -1 was recorded for the first and second male. Finally, the physical and clinicalreproductive evaluation concurred with the established parameters for the species.Limitations of the study / implications: Under the conditions in which the study wascarried out, it was not possible to evaluate males in the same age conditions andreproductive characteristics. However, this allowed collecting information from fieldconditions so that producers can apply it for herd improvement.Findings / conclusions: The male Creole goats of the evaluated herds showed intensesexual behavior with low sperm characteristics.

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Home sperm testing device versus laboratory sperm quality analyzer: comparison of motile sperm concentration

Fertility and Sterility ◽

10.1016/j.fertnstert.2018.08.049 ◽

2018 ◽

Vol 110 (7) ◽

pp. 1277-1284 ◽

Cited By ~ 21

Author(s):

Ashok Agarwal ◽

Manesh Kumar Panner Selvam ◽

Rakesh Sharma ◽

Kruyanshi Master ◽

Aditi Sharma ◽

...

Keyword(s):

Sperm Quality ◽

Sperm Concentration ◽

Testing Device ◽

Motile Sperm ◽

Quality Analyzer ◽

Sperm Quality Analyzer

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The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

World Nutrition Journal ◽

10.25220/wnj.v01i1.0007 ◽

2017 ◽

Vol 1 (1) ◽

pp. 28

Author(s):

Eviana Budiartanti Sutanto ◽

Taufiq R Nasihun ◽

Israhnanto Isradji ◽

Luciana Budiati Sutanto

Keyword(s):

Vitamin C ◽

Sperm Quality ◽

Sperm Concentration ◽

Control Group ◽

Control Group Design ◽

Normal Sperm ◽

Post Test ◽

Male Wistar Rats ◽

Post Hoc ◽

Per Oral

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in K2 (K2 32.59 million/mL, K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

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Effects of Chronic Musculoskeletal Pain on Fertility Potential in Lean and Overweight Male Patients

Pain Research and Management ◽

10.1155/2017/4628627 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10

Author(s):

Fereshteh Dardmeh ◽

Hiva Alipour ◽

Hans Ingolf Nielsen ◽

Sten Rasmussen ◽

Parisa Gazerani

Keyword(s):

Chronic Pain ◽

Musculoskeletal Pain ◽

Sperm Quality ◽

Sperm Concentration ◽

Negative Influence ◽

Reproductive Hormone ◽

Chronic Pain Patients ◽

Male Patients ◽

Fertility Potential ◽

Pain Patients

Both chronic pain and obesity are known to affect reproductive hormone profiles in male patients. However, the effect of these conditions, alone or in combination, on male fertility potential has received less attention. 20 chronic musculoskeletal pain patients and 20 healthy controls were divided into lean and overweight subgroups according to their BMI. Current level of chronic pain (visual analogue scale) and pressure pain thresholds (PPTs) in 16 predefined sites, classically described and tested as painful points on the lower body, were measured. Levels of reproductive hormone and lipid profiles were assessed by ELISA. Sperm concentration and motility parameters were analyzed using a computer-aided sperm analysis system. Sperm concentration, progressive motility, and percentage of hyperactivated sperm were generally lower in the chronic pain patients in both lean and overweight groups. The overweight control and the lean chronic pain groups demonstrated a significantly lower percentage of progressively motile sperm compared with the lean control group, suggesting that musculoskeletal chronic pain may have a negative influence on sperm quality in lean patients. However, due to the potential great negative influence of obesity on the sperm parameters, it is difficult to propose if musculoskeletal chronic pain also influenced sperm quality in overweight patients. Further research in chronic pain patients is required to test this hypothesis.

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P–031 The effect of ejaculatory abstinence period on embryological and clinical outcomes in ICSI cycles: A retrospective analysis of 3,353 cycles

Human Reproduction ◽

10.1093/humrep/deab130.030 ◽

2021 ◽

Vol 36 (Supplement_1) ◽

Author(s):

G C Cermisoni ◽

L Pagliardini ◽

A Alteri ◽

L D Santis ◽

S Esposito ◽

...

Keyword(s):

Clinical Outcomes ◽

Live Birth ◽

Sperm Quality ◽

Sperm Concentration ◽

Live Birth Rate ◽

Fertilization Rate ◽

Ongoing Pregnancy ◽

Abstinence Period ◽

Blastulation Rate ◽

Ejaculatory Abstinence

Abstract Study question Does ejaculatory abstinence period in male affect embryological and pregnancy outcomes following fresh embryo transfers in ICSI cycles? Summary answer Shorter ejaculatory abstinence period is associated with lower triploid zygotes rate per ICSI cycle but it does not affect clinical outcomes after fresh embryo transfers. What is known already Lower sperm quality may negatively impact on fertilisation rate and embryo morphokinetic parameters after ICSI and the effect of the ejaculatory abstinence period before semen collection on seminal parameters and sperm quality has been widely reported. However, the impact of ejaculatory abstinence on clinical outcomes is still controversial. WHO (World Health Organization) guideline recommended that abstinence period should be 2–7 days. Even so, there are no larger prospective trials determining the optimal timing for ejaculatory abstinence period for infertile couples. Study design, size, duration This is a single center retrospective observational study of 3,353 fresh cycles from January 2017 to December 2020. Semen analysis was done according to the WHO criteria. Exclusion criteria for this study were frozen gametes and cycles with no retrieved oocytes. Primary outcomes were fertilization rate and triploid zygotes rate. Secondary outcomes were blastulation rate, ongoing pregnancy rate and live birth rate per fresh embryo transfer. Participants/materials, setting, methods The correlation between ejaculatory abstinence and continuous outcomes was evaluated by Spearman’s correlation analysis in order to detect potential non-linear associations. Generalized linear model and logistic regression were used, respectively for continuous and binary outcomes, in order to adjust for confounders such as female age, male age, number of retrieved oocytes, percentage of mature oocytes, infertility causes, seminal volume, sperm concentration and total progressive sperm motility. A p value <0.05 was considered significant. Main results and the role of chance The male mean age was 40.3±5.5 and mean duration of abstinence was 2.9±1.7 days. The mean age of female patients was 38.2±4.0. Higher ejaculatory abstinence period was associated with a higher sperm concentration (Spearman p = 3.1x10–6) but not with a higher total sperm progressive motility. Even so, no significant correlation with EA were observed when considering fertilization rate, blastulation rate, ongoing pregnancy and live birth rate per transfer in analyzed cycles. Triploid zygote rate was positively associated with a higher ejaculatory abstinence period. For the ejaculatory abstinence period of 1 day (n = 64), 2 days (n = 1523), 3 days (n = 1032), 4 days (n = 408), 5 days (n = 174), 6 days (n = 47) and ≥7 days (n = 105) the mean triploid rate was 2.4%, 2.4%, 2.5%, 4.1%, 3.6%, 5.4% and 4.3%, respectively (Spearman p = 9x10–3). Triploid zygote rate was independent of semen volume, concentration and total progressive motility. Limitations, reasons for caution This is a large observational study with a retrospective data collection. Despite our methodological approach, the presence of biases related to retrospective design can not be excluded and it may be a reason for caution. Wider implications of the findings: Our results demonstrate that ejaculatory abstinence period do not affect blastulation, ongoing pregnancy and live birth rates. The current findings discourage an abstinence time longer than 3 days due to its association with a higher abnormal fertilization rate. Trial registration number Not applicable

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Factors affecting the response of working boars to changing environmental temperature

Proceedings of the British Society of Animal Production (1972) ◽

10.1017/s0308229600020900 ◽

1991 ◽

Vol 1991 ◽

pp. 140-140

Author(s):

Angela H Cliff ◽

J H Dunne ◽

P R English ◽

J S M Hutchinson ◽

O Macpherson

Keyword(s):

Heat Stress ◽

Adverse Effects ◽

Environmental Temperature ◽

Semen Quality ◽

Sperm Quality ◽

Sperm Concentration ◽

Factors Affecting ◽

High Environmental Temperature ◽

Sudden Rise ◽

Sperm Output

It is well established that high environmental temperature can have adverse effects on sperm concentration and the normality of sperm in the boar. Induced abnormalities include reduced motility, abnormal heads, proximal droplets, coiled and bent tails and abnormal acrosomes (Malmgren and Larsson, 1989). While all stages of spermatogenesis can be adversely affected, the primary spermatocytes are most vulnerable to these influences. Since spermatogenesis occurs over 45 days, any adverse effects of elevated ambient temperature can affect sperm quality for around 6 weeks. The maximum period of infertility appears around weeks 3 and 4 after heat stress. Reduced levels of testosterone and sometimes of LH following heat stress are implicated in these adverse effects. There is evidence that boars subjected to high constant temperature (30°C), which have become acclimatised to such, are affected less in terms of sperm output and quality (Cameron and Blackshaw, 1980) than when boars are subject to sudden major increases in temperature from fairly low levels (15 to 30 C) e.g. during the summer months (Antonyuk et al, 1983). There also appears to be large differences between boars in their ability to adapt to exposure to high environmental temperature by minimising temperature rise and avoiding adverse effects on semen quality (Cameron and Blackshaw, 1980). These workers found that boars prone to heat stress show an acute rise in body temperature in response to elevated environmental temperature and this sudden rise has a more adverse effect on semen quality than the length of exposure to the elevated temperature. There appears to be little information available on the reasons for such important between boar differences. This study was carried out to determine rectal temperature responses of boars to varying summer temperature in an intensive pig enterprise in Scotland and to attempt to determine some of the factors associated with ‘high’ and ‘low’ responding boars.

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The Association Between Race, Obesity, and Sperm Quality Among Men Attending a University Physician Practice in Washington, DC

American Journal of Men s Health ◽

10.1177/1557988320925985 ◽

2020 ◽

Vol 14 (3) ◽

pp. 155798832092598

Author(s):

Nathan L. McCray ◽

Heather A. Young ◽

Michael S. Irwig ◽

David Frankfurter ◽

Arnold M. Schwartz ◽

...

Keyword(s):

Semen Quality ◽

Sperm Quality ◽

Sperm Count ◽

Sperm Concentration ◽

World Health ◽

Washington Dc ◽

Physician Practice ◽

Research Attention ◽

Men Of Color ◽

Hispanic Men

A decades-long decline in sperm counts in Western countries has coincided with an increase in obesity rates, prompting study into their association. Few of these studies have incorporated men of color, the sperm health of whom is relatively unknown. The present exploratory study evaluated the association between body mass index (BMI), race, ethnicity, and sperm parameters among a diverse sample of U.S. men attending a Washington, DC physician practice. Semen samples were collected and processed at a single laboratory and sperm concentration, motility, morphology, and count were evaluated according to World Health Organization (WHO) 5th edition criteria. Multivariate models accounted for covariates related to sperm health. The study population ( n = 128) was largely obese (45.3%) or overweight (34.4%), and 36.0% were black or Hispanic. Black men had lower adjusted sperm concentration compared to white men (75.0 million/mL to 107.4 million/mL, p = .01) and were more likely to have oligozoospermia ( p = .01), asthenozoospermia ( p = .004), and low sperm count ( p < .0001). Hispanic men had higher adjusted sperm concentration compared to non-Hispanic men (124.5 million/mL to 62.1 million/mL, p = .007) and were less likely to have teratozoospermia ( p = .001). Obesity and BMI were associated with lower sperm motility and count in crude models only. Given the study’s sample size its findings should be interpreted with caution but align with the limited epidemiological literature to date that has evaluated racial and ethnic differences in semen quality. Heightened clinical research attention is needed to ensure men of color are included in representative numbers in studies of urologic and andrologic health.

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A COMPARISON OF CROSSBRED AND PUREBRED BOARS FOR SEMEN AND REPRODUCTIVE CHARACTERISTICS

Canadian Journal of Animal Science ◽

10.4141/cjas78-009 ◽

1978 ◽

Vol 58 (1) ◽

pp. 63-70 ◽

Cited By ~ 10

Author(s):

P. D. CONLON ◽

B. W. KENNEDY

Keyword(s):

Reproductive Performance ◽

Sperm Concentration ◽

Conception Rate ◽

Reproductive Characteristics ◽

Rate Score ◽

Double Mating ◽

Semen Volume ◽

Semen Characteristics

Crossbred Hampshire–Duroc boars were compared to purebred Hampshire, Duroc and Landrace boars for seven semen characteristics and for reproductive performance under competitive double mating. A total of 14 boars were represented: three Hampshire–Duroc, three Hampshire, three Duroc and five Landrace. Significant breed of boar effects were observed on semen volume (P <.01), sperm concentration (P <.10), concentration score (P <.05), morphology score (P <.10), live–dead rate score (P <.05) and total semen score (P <.01). Landrace and Hampshire–Duroc boars produced more than twice the volume of semen produced by purebred Hampshires and Durocs. Hampshire, Duroc and Hampshire–Duroc boars had greater sperm concentrations and concentration scores than Landrace. Hampshire–Duroc boars ranked highest for morphology and total semen scores, but lowest for live–dead rate score. When double-mated to Landrace sows, breed of boar had no significant effect on conception rate but significantly affected (P <.05) percent of litter sired. Hampshire, Duroc and Hampshire–Duroc boars sired almost twice as many pigs as the Landrace.

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FSH treatment of male idiopathic infertility improves pregnancy rate: a meta-analysis

Endocrine Connections ◽

10.1530/ec-15-0050 ◽

2015 ◽

Vol 4 (3) ◽

pp. R46-R58 ◽

Cited By ~ 41

Author(s):

D Santi ◽

A R M Granata ◽

M Simoni

Keyword(s):

Clinical Trials ◽

Pregnancy Rate ◽

Sperm Quality ◽

Meta Analysis ◽

Male Partner ◽

Sperm Concentration ◽

Controlled Clinical Trials ◽

Idiopathic Infertility ◽

Reproductive Techniques ◽

Infertile Couples

IntroductionThe aim of this study is to comprehensively evaluate whether FSH administration to the male partner of infertile couples improves pregnancy rate, spontaneously and/or after assisted reproductive techniques (ART).MethodsMeta-analysis of controlled clinical trials in which FSH was administered for male idiopathic infertility, compared with placebo or no treatment. Randomization was not considered as an inclusion criterion.ResultsWe found 15 controlled clinical studies (614 men treated with FSH and 661 treated with placebo or untreated). Concerning the type of FSH, eight studies used recombinant FSH, whereas seven studies used purified FSH. Nine studies evaluated spontaneous pregnancy rate, resulting in an overall odds ratio (OR) of about 4.5 (CI: 2.17–9.33). Eight studies evaluated pregnancy rate after ART, showing a significant OR of 1.60 (CI: 1.08–2.37). Sub-dividing studies according to the FSH preparations (purified/recombinant), pregnancy rate improvement remained significant for each preparation. Eleven studies considered sperm quality after FSH treatment, finding a significant improvement of sperm concentration (2.66×106/ml, CI: 0.47–4.84), but not of concentration of sperm with progressive motility (1.22×106/ml, CI: −0.07 to 2.52). Three trials evaluated testicular volume, showing a non-significant increase in men treated (1.35 ml, CI: −0.44 to 3.14).ConclusionThe results of controlled clinical trials available in the literature indicate an improvement of pregnancy rate after FSH administration to the male partner of infertile couples, both spontaneously and after ART. However, the heterogeneity of studies, the high risk of bias and the lack of precise criteria to guide FSH administration limit the strength of these results. Future studies should be designed to identify the markers of FSH response which are helpful in the decision-making process. Meanwhile, the use of FSH in the treatment of male infertility should be cautious.

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294 EFFECT OF SPERMATOZOA MOTILITY ON TRANSFERABLE EMBRYO RATE OF SUPEROVULATED JAPANESE BLACK CATTLE

Reproduction Fertility and Development ◽

10.1071/rdv21n1ab294 ◽

2009 ◽

Vol 21 (1) ◽

pp. 244

Author(s):

K. Hayama ◽

M. Takeuchi ◽

A. Ideta ◽

M. Urakawa ◽

M. Sasatani ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Quality ◽

Sperm Concentration ◽

Electronic Systems ◽

Embryo Production ◽

Spermatozoa Motility ◽

Multiple Comparison Test ◽

Medical Electronic ◽

D 20

Sperm motility is known to affect fertilization; however, little is known about the relationship between frozen–thawed sperm motility and in vivo fertilization following superovulatory treatment. The objective of this study was to evaluate a sperm function test as potential predictors of embryo production following superovulatory treatment in cattle. Two to five batches of semen (Japanese black bull, n = 4, A to D) were diluted with egg york-citrate-glycerol in 0.5 mL plastic straws, and they were stored in liquid nitrogen until analyzed. Frozen–thawed spermatozoa were evaluated for motility {motile sperm concentration (MSC, million mL–1), progressive MSC (PMSC, million mL–1) and velocity (μm s–1)} using a sperm quality analyzer for bulls (SQA-Vb, Medical Electronic Systems, Caesarea, Israel). Each sample of 20 μL aspirated into the disposable capillary, was inserted into SQA-Vb. Measurements were displayed within 75 s. Intra-assay CVs of MSC, PMSC, and velocity were 14.2, 7.3 and 7.5%, respectively. Inter-assey CVs of them were 13.5, 3.9 and 4.3% respectively. Superstimulated donors (Japanese black cows, n = 161) were artificially inseminated with one dose of frozen–thawed semen (bull A = 74, B = 46, C = 21 and D = 20). The proportion of transferable embryo (IETS grade 1 to 3) was examined on day 7 (day 0 = estrus). Data were analyzed using ANOVA followed by Scheffe multiple comparison test, and Fisher’s z-transformation. MSC, PMSC and velocity values differed significantly among each bull. The values of bull A were much lower than those of the other bulls. The proportion of transferable embryos produced by bull A was significantly lower than that of other bulls (P < 0.05, Table 1). Correlations showed significant association between MSC and proportion of transferable embryos (r = 0.99, P < 0.01). We conclude that bovine sperm motility using a SQA-Vb is a useful predictor of embryo production following superovulatory treatment. Table 1.Relationship between sperm motility and proportion of transferable embryo

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154 A method of oviductal semen deposition for use in the goat

Reproduction Fertility and Development ◽

10.1071/rdv32n2ab154 ◽

2020 ◽

Vol 32 (2) ◽

pp. 203

Author(s):

N. Buzzell ◽

S. Blash ◽

K. Miner ◽

M. Schofield ◽

J. Pollock ◽

...

Keyword(s):

Assisted Reproductive Technologies ◽

Sperm Quality ◽

Sperm Concentration ◽

The United States ◽

Reproductive Technologies ◽

Poor Quality ◽

Implant Removal ◽

Suitable Alternative ◽

Midline Laparotomy ◽

Post Surgery

The objective of this study was to investigate a method of oviducal semen deposition as a strategy for producing offspring from poor-quality cryopreserved goat sperm. Invitro fertilisation (IVF) and AI are common assisted reproductive technologies used in small ruminants, but they have varied results in the goat. The use of poor-quality cryopreserved-thawed sperm (<50% live/dead ratio at post-thaw) can decrease the rate of success. These procedures were performed in the month of November in Central Massachusetts in the United States (42° N). Seven 10-year-old dairy goats (Saanen, Toggenburg, and Alpine breeds) were synchronised and superovulated using a progesterone implant on Day 0, a prostaglandin injection at Day 7, two daily injections of 36mg of FSH ~12h apart on Days 12-15, and progesterone implant removal on Day 14 followed by an injection of 50µg of gonadotrophin-releasing hormone. Sperm deposition was performed on Day 17 (72 h after implant removal). The animals were anaesthetised using a standardised protocol, intubated, and maintained using isoflurane, and sterile prep was performed before a midline laparotomy procedure. Straws from a single ejaculate from a transgenic founder that was cryopreserved using a commercial two-step glycerol-egg yolk-based extender were used. A straw from this collection was post-thawed 30 days after collection and, using a commercial live/dead stain, 67% live sperm was determined. The optimal type of sperm prep and sperm concentration is unknown and may be dependent on sperm quality. Therefore, different gradient preps using Vitrolife SpermGrad at three volumes (1.5 (used on two animals), 1.0, and 0.5mL) as well as two volumes of IVF Bioscience Bovine BO-SemenPrep (4.0mL (used on two animals) and 2.0mL) were used. All five pellets were diluted in 1.0mL of IVF Bioscience Bovine BO-IVF media. Sperm concentrations ranging from 75×106 to 27×106 spermmL−1 were deposited into one oviduct; then, a 10:1 dilution was performed and 7.5×106 to 2.7×10 spermmL−1 were deposited into the contralateral oviduct. The depositions were performed just proximal to the uterotubal junction in a volume of 0.1mL of diluent via a tuberculin syringe attached to a 20-gauge needle. Two days following the procedure, oviducts were flushed postmortem from three of the seven randomly selected goats. All three had fertilised embryos, and nineteen 8-cell embryos were retrieved. Three of these embryos were surgically transferred to the distal uterine horn of a suitable recipient. The recipient became pregnant and produced a single offspring. The remaining four of seven goats were killed 41 days post-surgery. Two of the four goats were pregnant, with one carrying one fetus and the other carrying five fetuses. Further studies are needed to optimise this method, but these initial results indicate that oviducal semen deposition directly into the oviduct proximal to the uterotubal junction may be a suitable alternative for producing offspring from suboptimal cryopreserved-thawed goat sperm.

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