scholarly journals Periodontal Status and Subgingival Biofilms in Cystic Fibrosis Adults

2019 ◽  
Vol 68 (3) ◽  
pp. 377-382 ◽  
Author(s):  
TAMARA PAWLACZYK-KAMIEŃSKA ◽  
RENATA ŚNIATAŁA ◽  
HALINA BATURA-GABRYEL ◽  
MARIA BORYSEWICZ-LEWICKA ◽  
SZCZEPAN COFTA
Keyword(s):  
Cystic Fibrosis ◽  
Clinical Symptoms ◽  
Periodontal Diseases ◽  
Adult Patients ◽  
Periodontal Pathogens ◽  
Rt Pcr ◽  
Pocket Depth ◽  
Periodontal Status ◽  
Periodontal Tissues ◽  
Probing Pocket Depth

The aim of this study was to assess the periodontal status of cystic fibrosis (CF) adult patients and to evaluate whether there is a correlation between the bacterial population of the subgingival biofilm and the health status of the periodontal tissues in this group of adults. The study involved 22 cystic fibrosis adult patients. The periodontal condition was assessed using Plaque Index (PLI), Gingival Index (GI), and Probing Pocket Depth (PPD). The gingival sulcus samples were analyzed by the Real-Time PCR assay (RT-PCR). Majority of patients showed moderate or severe bacterial dental plaque accumulation, but none of them had clinical symptoms of periodontal diseases. RT-PCR showed the presence of periopathogens in 50% of patients. Red complex microorganisms were detected in 9.09%, orange complex in 27.27%, and green complex in 31.82% of the samples analyzed. In cystic fibrosis patients colonized by periopathogens, the periodontal markers were significantly higher in comparison to not colonized by periopathogens patients. Despite the widespread presence of bacterial dental deposits in the cystic fibrosis adult patients examined, none of them has clinical symptoms of periodontal disease; however, the presence of periodontal pathogens in subgingival biofilm may represent a possible risk factor of this disease in the future. An unsatisfactory level of oral hygiene in any patient with cystic fibrosis indicates a need to focus on standards of dental care for such patients.

scholarly journals Toward Personalized Oral Diagnosis: Distinct Microbiome Clusters in Periodontitis Biofilms

2021 ◽  
Vol 11 ◽  
Author(s):  
Roland Wirth ◽  
Bernadett Pap ◽  
Gergely Maróti ◽  
Péter Vályi ◽  
Laura Komlósi ◽  
...  
Keyword(s):  
Amplicon Sequencing ◽  
Abundant Species ◽  
Periodontal Pathogen ◽  
Host Immune System ◽  
Periodontal Pathogens ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
The Individual ◽  
Biofilm Development

Periodontitis is caused by pathogenic subgingival microbial biofilm development and dysbiotic interactions between host and hosted microbes. A thorough characterization of the subgingival biofilms by deep amplicon sequencing of 121 individual periodontitis pockets of nine patients and whole metagenomic analysis of the saliva microbial community of the same subjects were carried out. Two biofilm sampling methods yielded similar microbial compositions. Taxonomic mapping of all biofilms revealed three distinct microbial clusters. Two clinical diagnostic parameters, probing pocket depth (PPD) and clinical attachment level (CAL), correlated with the cluster mapping. The dysbiotic microbiomes were less diverse than the apparently healthy ones of the same subjects. The most abundant periodontal pathogens were also present in the saliva, although in different representations. The single abundant species Tannerella forsythia was found in the diseased pockets in about 16–17-fold in excess relative to the clinically healthy sulcus, making it suitable as an indicator of periodontitis biofilms. The discrete microbial communities indicate strong selection by the host immune system and allow the design of targeted antibiotic treatment selective against the main periodontal pathogen(s) in the individual patients.

scholarly journals Penggunaan Asam Lemak Omega-3 sebagai Terapi Pendukung dalam Perawatan Periodontitis

e-GIGI ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 209
Author(s):  
Pingkan A. Lengkong ◽  
Johanna A. Khoman ◽  
Pritartha S. Anindita
Keyword(s):  
Fatty Acids ◽  
Supportive Therapy ◽  
Periodontal Tissue ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Pocket Depth ◽  
Periodontal Tissues ◽  
Probing Pocket Depth ◽  
Surgical Methods ◽  
Randomized Control

Abstract: Periodontitis is an inflammation of periodontal tissue characterized by clinical attachment loss, periodontal pocket formation, gingival recession, and periodontal tissue destruction. Periodontitis can be treated by surgical or non-surgical methods. One of the non-surgical methods is host modulation therapy which can reduce the damage by treating the chronic inflammatory response. Host modulation therapy is in the form of drugs or supplements that have anti-inflammatory properties. Omega-3 fatty acids have anti-inflammatory properties that serve as a protection against inflammation and infection including periodontitis. This study was aimed to obtain the effect of using omega-3 fatty acids as supportive therapy in the treatment of periodontitis. This was a literature review study by using PubMed, Clinical Key and Google Scholar databases. The keywords used were omega-3 fatty acids and periodontitis. After being selected based on inclusion and exclusion criteria, a critical appraisal was carried out to obtain seven randomized control trial literature. The results showed that the use of omega-3 fatty acids had an effect on the mean value of decreasing probing pocket depth and increasing clinical attachment of periodontal tissues. In conclusion, omega-3 fatty acids have an effect and can be used as a supportive therapy in the treatment of periodontitis. Omega-3 fatty acids lead to a better increase in the resolution of inflammation and can accelerate the healing of periodontal tissues by decreasing probing pocket depth and increasing clinical attachment.Keywords: omega-3 fatty acids; periodontal tissue; periodontitis  Abstrak: Periodontitis adalah inflamasi jaringan periodontal yang ditandai dengan kehilangan perlekatan klinis, pembentukan saku periodontal, resesi gingiva, dan kerusakan jaringan peridontal. Periodontitis dapat diobati dengan metode bedah maupun non bedah. Salah satu metode non bedah yaitu terapi modulasi host yang dapat mengurangi kerusakan dengan mengobati aspek respons inflamasi kronis. Terapi modulasi host berupa pemberian obat maupun suplemen yang memiliki sifat anti inflamasi. Asam lemak omega-3 memiliki sifat anti inflamasi yang berfungsi sebagai perlindungan dalam melawan peradangan dan infeksi termasuk periodontitis. Penelitian ini bertujuan untuk mengetahui gambaran pengaruh penggunaan asam lemak omega-3 sebagai terapi pendukung dalam perawatan periodontitis. Jenis penelitian ialah suatu literature review menggunakan database PubMed, Clinical Key. dan Google Scholar. Kata kunci yang digunakan yaitu omega-3 fatty acids and periodontitis. Setelah diseleksi berdasarkan kriteria inklusi dan eksklusi, dilakukan critical appraisal dan didapatkan tujuh literatur randomized control trial. Hasil penelitian mendapatkan bahwa penggunaan asam lemak omega-3 memengaruhi nilai rerata penurunan kedalaman probing poket dan meningkatkan perlekatan klinis jaringan periodontal. Simpulan penelitian ini ialah asam lemak omega-3 memiliki pengaruh dan dapat digunakan sebagai terapi pendukung dalam perawatan periodontitis. Asam lemak omega-3 menyebabkan peningkatan yang lebih baik dalam resolusi peradangan dan dapat mempercepat penyembuhan jaringan periodontal dilihat dari penurunan kedalaman probing poket dan meningkatnya perlekatan klinis.Kata kunci: asam lemak omega-3; jaringan periodontal; periodontitis

scholarly journals Detection of pathogens from periodontal lesions

2004 ◽  
Vol 38 (5) ◽  
pp. 723-728 ◽  
Author(s):  
Veruska de João Malheiros ◽  
Mario Julio Avila-Campos
Keyword(s):  
Healthy Subject ◽  
Sensitivity And Specificity ◽  
Diagnostic Tool ◽  
Healthy Subjects ◽  
Periodontal Diseases ◽  
Adult Patients ◽  
Clinical Samples ◽  
Periodontal Pathogens ◽  
Biochemical Tests ◽  
Bacteria Identification

OBJECTIVE: To comparatively detect A. actinomycetemcomitans and F. nucleatum from periodontal and healthy sites. METHODS: Subgingival clinical samples from 50 periodontitis adult patients and 50 healthy subjects were analyzed. Both organisms were isolated using a trypticase soy agar-bacitracin-vancomycin (TSBV) medium and detected by PCR. Conventional biochemical tests were used for bacteria identification. RESULTS: A. actinomycetemcomitans and F. nucleatum were isolated in 18% and 20% of the patients, respectively, and in 2% and 24% of healthy subjects. Among A. actinomycetemcomitans isolates, biotype II was the most prevalent. Primer pair AA was 100% sensitive in the detection of A. actinomycetemcomitans from both subject groups. Primers ASH and FU were also 100% sensitive to detect this organism in healthy subject samples. Primer pair FN5047 was more sensitive to detect F. nucleatum in patients or in healthy samples than primer 5059S. Primers ASH and 5059S were more specific in the detection of A. actinomycetemcomitans and F. nucleatum, respectively, in patients and in healthy subject samples. CONCLUSIONS: PCR is an effective tool for detecting periodontal pathogens in subgingival samples, providing a faster and safer diagnostic tool of periodontal diseases. The method's sensitivity and specificity is conditioned by the choice of the set of primers used.

scholarly journals Psychosocial Stress and its Effect on Periodontal Tissues using Malondialdehyde as Oxidative Stress Biomarker

2018 ◽  
Vol 2 (2) ◽  
pp. 34-39
Author(s):  
Anuradha Mokashi ◽  
Keshava Abbayya ◽  
Siddhartha Varma ◽  
Sameer Zope ◽  
Girish Sugarimath ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Psychosocial Stress ◽  
Inflammatory Diseases ◽  
Periodontal Diseases ◽  
Stress Index ◽  
Pocket Depth ◽  
Cross Sectional ◽  
Periodontal Tissues ◽  
Stress Biomarker ◽  
Oxidative Stress Biomarker

Background: Stress is playing a major role as an aetiologic factor in the initiation of many systemic diseases. Similarly, it is also thought to be an aetiologic factor in the progression of periodontal diseases. Aim: The production of free radical species is associated with various inflammatory diseases and also during stressful conditions. Hence, an effort in the present study is done to correlate psychosocial stress and periodontitis using serum MDA as an oxidative stress biomarker. Materials and Methods: A current cross-sectional study included 201 individuals between 20 to 60 years of age. A periodontal examination included Probing Pocket Depth, CAL, Plaque Index, and Gingival Index. According to CAL individuals were divided into four groups i.e. healthy, slight (CAL = 1-2 mm), moderate (CAL = 3-4 mm) and severe (CAL = ≥5 mm) periodontitis. Psychosocial stress of the individuals was evaluated using occupational stress index. Serum MDA level was evaluated using spectrochromatometer. Results: Patients with stress demonstrated increased levels of MDA along with a higher loss of attachment. Conclusion: The results revealed statistically significant association between psychosocial stress and periodontitis indicating psychosocial stress as a risk factor for developing periodontitis.

scholarly journals MICRO BIOLOGY OF PERIODONTAL DISEASE- A REVIEW

2021 ◽  
Vol 5 (6) ◽  
Author(s):  
Jageer Chinna ◽  
Jannat Sharma
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Disease Process ◽  
Fusobacterium Nucleatum ◽  
Host Cells ◽  
Bacterial Invasion ◽  
Periodontal Pathogens ◽  
Tissue Destruction ◽  
Campylobacter Rectus ◽  
Periodontal Tissues

Periodontal diseases are inflammatory and destructive diseases of the dentogingival complex associated with specific periodontal pathogens inhabiting periodontal pockets. Periodontal diseases lead to damage of the periodontal tissues supporting the teeth (bone and connective tissue) and affect the quality of life of the affected individuals: poor alimentation, tooth loss, social and financial problems. Although it is generally considered that the disease has multifactorial etiology, data show that some specific Gram-negative microorganisms in the subgingival plaque biofilm play a major role in the initiation and progression of periodontitis. Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia form a consortium in the subgingival biofilm and are regarded as the principal periodontopathogenic bacteria. Other microorganisms that have been implicated as predominant species in the disease process are: Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Campylobacter rectus, Peptostreptococcus migros, Eikenella corrodens. In periodontitis, the initiation of the disease is the colonization of the tissues by these pathogenic species. The next step is bacterial invasion or invasion by pathogenic products into the periodontal tissues, interactions of bacteria or their substances with host cells, and this directly/indirectly causes degradation of the periodontium, resulting in tissue destruction. Keywords: periodontal disease, periodontal pathogens, microbiology.

Evaluation of hemomicrocirculation of periodontal tissues in localized periodontitis of traumatic etiology

2021 ◽  
Vol 26 (1) ◽  
pp. 73-79
Author(s):  
A. V. Smirnova ◽  
Iu. F. Semiglazova ◽  
D. A. Kuzmina
Keyword(s):  
Cardiovascular Diseases ◽  
Periodontal Diseases ◽  
Functional Condition ◽  
The Body ◽  
Periodontal Pocket ◽  
Pocket Depth ◽  
Periodontal Tissues ◽  
Gastrointestinal Problems ◽  
Frequent Relapses ◽  
Adaptive Reaction

Relevance. The necessity of somatic health evaluation in patients with local inflammatory periodontal processes is determined by frequent relapses of the periodontal diseases. Low effectiveness of the widespread treatment methods and the lack of long-term remission of the disease is caused by the insufficient assessment Based on the clinical examination, diagnosis and laboratory tests, to determine the correlation between the somatic pathology and adaptability of the adult body in patients with localized periodontitis due to trauma. Materials and methods. Advanced examination was carried out in 169 subjects, aged 45-75 y.o., with mild to moderate localized periodontitis due to trauma. The somatic condition was assessed by laboratory and functional tests. Results. 1-2 somatic diseases were revealed in 39.3% of patients with mild localized periodontitis caused by trauma (periodontal pocket depth up to 4 mm). 3 and more comorbidities were detected in 35.3% of patients. Gastrointestinal problems, cardiovascular diseases, allergic conditions, anemiа and respiratory diseases were most frequently encountered. In moderate localized periodontitis caused by trauma and periodontal depth of 4-6 mm, 23.9% of patients had 1-2 comorbidities, while 62.7% of patients had 3 and more diseases. Gastrointestinal problems, cardiovascular diseases, anemia, allergic conditions and urogenital diseases were more frequent. A strong correlation was revealed between the type of adaptive reaction and the degree of localized periodontitis caused by trauma. Thus, the risk of moderate localized traumatic periodontitis development increases by 1.8 times in tense adaptive reaction, and by 3.45 times - in poor adaptation reaction. Conclusion. Functional condition of the body adaptability, which is essential for the development of the treatment strategy, could be determined by the evaluation of the physical condition, peripheral blood tests (entropy of WBC differential, C-reactive protein) in patients with localized periodontitis caused by trauma. The adaptive reaction tension and decrease were detected in patients with 3 or more comorbidities.

scholarly journals The Effects of Antimicrobial Peptide Nal-P-113 on Inhibiting Periodontal Pathogens and Improving Periodontal Status

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Hongyan Wang ◽  
Lisi Ai ◽  
Yu Zhang ◽  
Jyawei Cheng ◽  
Huiyuan Yu ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Alveolar Bone ◽  
Systemic Disease ◽  
Treatment Method ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Growth Curve Analysis ◽  
Periodontal Pathogens ◽  
Pocket Depth ◽  
Periodontal Status

Periodontal disease consists of chronic gingival inflammation characterized by both degradation of the periodontal connective tissue and alveolar bone loss. Drug therapy is used as an auxiliary treatment method in severe chronic periodontitis, aggressive periodontitis, and periodontitis-associated systemic disease. Nal-P-113, a modified antimicrobial peptide, specifically replaces the histidine residues of P-113 with the bulky amino acidβ-naphthylalanine, and our previous studies have verified that this novel peptide is not toxic to the human body within a certain concentration range. The objective of the present study was to evaluate the effect of Nal-P-113 on periodontal pathogens and periodontal status in clinical studies. In a split-mouth clinical trial, the pocket depth and bleeding index values tended to decrease in the experimental group compared with those in the control group. SEM results verified that Nal-P-113 restrained the maturation of plaque. Based on real-time polymerase chain reaction, the levels ofFusobacterium nucleatum,Streptococcus gordonii,Treponema denticola,andPorphyromonas gingivalisin subgingival plaque were decreased when the subjects were given Nal-P-113. Bacterial growth curve analysis and a biofilm susceptibility assay verified that Nal-P-113 at a concentration of 20 μg/mL restrained the growth ofS. gordonii,F. nucleatum,andP. gingivalisand biofilm formation. Therefore, Nal-P-113 effectively reduces periodontal pathogens and ameliorates periodontal status.

scholarly journals The Assessment of Periodontal Status and Treatment Needs of the Adult Population Aged 34-44 Residing in Lublin Province and Visiting the Dentist Regularly

2014 ◽  
Vol 124 (2) ◽  
pp. 86-88
Author(s):  
Małgorzata Stodókiewicz ◽  
Joanna Krawczyk ◽  
Jacek Szkutnik ◽  
Marcin Berger
Keyword(s):  
Alveolar Bone ◽  
Periodontal Diseases ◽  
Adult Population ◽  
World Health ◽  
Treatment Needs ◽  
Periodontal Status ◽  
Periodontal Tissues ◽  
Periodontal Index ◽  
Community Periodontal Index ◽  
Health Organization

Abstract Introduction. Periodontitis is a group of inflammatory disorders affecting periodontal tissues. This condition manifests by a progressive destruction of the alveolar bone, subsequently leading to tooth loss. World Health Organization introduced Community Periodontal Index of Treatment Needs in order to gain data regarding periodontal health and treatment needs of people with periodontitis Aim. To evaluate the periodontal status of citizens living in the city of Lublin and its surrounding, using Community Periodontal Index of Treatment Needs (CPITN). Material and methods. Community Periodontal Index of Treatment Needs was used to assess the periodontal status among 180 patients aged 35-44 residing in Lublin and the area around it. Results. Periodontal diseases have been observed in over 90% of the examined population. Treatment need index TN1 has referred to 26.11% of the patients, TN2 – 61.67% and TN3 – 2.22% respectively. Conclusions. Patients who visit the dentist regularly have a better periodontal status as compared to groups randomly selected.

Assessment of the Plasma/Serum IgG Test to Screen for Periodontitis

2012 ◽  
Vol 91 (12) ◽  
pp. 1190-1195 ◽  
Author(s):  
C. Kudo ◽  
K. Naruishi ◽  
H. Maeda ◽  
Y. Abiko ◽  
T. Hino ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Antibody Titer ◽  
Chronic Periodontitis ◽  
Characteristic Curve ◽  
Periodontal Treatment ◽  
Clinical Parameters ◽  
Periodontal Pathogens ◽  
Igg Antibody ◽  
Pocket Depth ◽  
Probing Pocket Depth

Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients is essential for general health. This study was performed to evaluate prospectively the diagnostic utility of a blood IgG antibody titer test against periodontal pathogens. Oral examination was performed, and IgG titers against periodontal pathogens were measured by ELISA in 1,387 individuals. The cut-off value of the IgG titer was determined in receiver operating characteristic curve analysis, and changes in periodontal clinical parameters and IgG titers by periodontal treatment were evaluated. The relationships between IgG titers and severity of periodontitis were analyzed. The best cut-off value of IgG titer against Porphyromonas gingivalis for screening periodontitis was 1.682. Both clinical parameters and IgG titers decreased significantly under periodontal treatment. IgG titers of periodontitis patients were significantly higher than those of healthy controls, especially in those with sites of probing pocket depth over 4 mm. Multiplied cut-off values were useful to select patients with severe periodontitis. A blood IgG antibody titer test for Porphyromonas gingivalis is useful to screen hitherto chronic periodontitis patients (ClinicalTrials.gov number NCT01658475).

scholarly journals Nature’s Anti-inflammatory agent and its use as a Local drug delivery agent for treatment of chronic periodontitis: A clinical, microbiological and biochemical study

2021 ◽  
Vol 7 (1) ◽  
pp. 30-35
Author(s):  
Vandana Kangowkar Vijayapremakumar ◽  
◽  
Agami Mehta ◽  
Priyanka Shivanand ◽  
Shobha Prakash ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Antimicrobial Agents ◽  
Alveolar Bone ◽  
Local Drug Delivery ◽  
Control Group ◽  
Periodontal Pathogens ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Significant Difference ◽  
Anti Inflammatory

Periodontal disease can lead to progressive loss of tooth-supporting tissues and alveolar bone. Due to the clinical limitations of scaling and root planing and recolonization of bacteria, the use of systemic and local administration of antimicrobial agents as adjuncts seems beneficial. In recent years, herbal and ayurvedic remedies are being researched to treat common infections and inflammatory conditions. Here an attempt was made to evaluate the effect of curcumin 10mg (Curenext) (CU) used as an adjunct to non-surgical periodontal therapy. A total of 10-15 sites in 14 patients with probing pocket depth 5-7mm were included. In experimental group, sites were treated with SRP+CU and in control group sites were treated with SRP alone. Plaque index, gingival bleeding index, gingival index, probing pocket depth and clinical attachment level were assessed at baseline, 21st day, 30th day, and 90th day. Subgingival plaque samples were collected to assess periodontal pathogens like Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum by anaerobic culture. GCF samples were collected to assess lactate dehydrogenase at baseline and 21st day. Results showed significant reduction in clinical parameters (PI, GBI, GI, PPD and CAL) and high statistically significant reduction in periodontal pathogens and lactate dehydrogenase in both the treatment groups, significant difference was seen in SRP+CU group. To conclude, the adjunctive use of curcumin 10mg (curenext* ) as an anti-inflammatory, antimicrobial, and antiplaque agent along with routine mechanical debridement is definitely a promising therapy that would add to the potential benefits of the periodontal treatment.

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