scholarly journals Use of the automated cell counter Luna-II for estimating the abundance of marine microalgae cells and their sizes in cultures

2020 ◽  
pp. 90-95
Author(s):  
L.V. Stelmakh ◽  
◽  
I.I. Babich ◽  
Keyword(s):  
Single Cells ◽  
Marine Microalgae ◽  
Main Method ◽  
Culture Cells ◽  
Sphericity Index ◽  
Accuracy Of Measurements ◽  
Pure Cultures ◽  
Basic And Applied Research ◽  
Automated Cell Counter

Marine microalgae isolated in algologically pure cultures are widely used for basic and applied research. All studies related to microalgae are accompanied by an assessment of their abundance and cell size. The main method for determining these parameters is light microscopy. However, this method is quite time-consuming, therefore, at present, various kinds of automatic particle counters are increasingly being used. This significantly reduces the processing time of microalgae samples and increases the accuracy of measurements. The purpose of this work is to evaluate the possibility of using an automatic particle counter Luna-II to find out the abundance of cells of certain types of marine microalgae and determine their linear sizes. The performed work allowed us to show the possibility of using this counter to quickly record the abundance of culture cells of marine microalgae and determine their linear sizes with high accuracy. Reliable results can be obtained for dense cell suspensions represented by single cells with a low percentage of doublets and triplets (not more than 3%) and a high sphericity index (above 0,95). For algal cultures, the cells of which have an average and low sphericity index, reliable results can only be obtained by the abundance of cells, provided that there is a slight presence of sticking cells (doublets and triplets) in the suspension. The presented automatic counter can be used when conducting ecological and physiological studies on the cultures of certain types of marine planktonic algae, as well as in monitoring activities related to assessing the quality of the aquatic environment using microalgae as bioassays.

Experience of clinical testing of personal telemedicine system ‘Obereg’ for remote monitoring of patients in intensive care units

2020 ◽  
pp. 52-58 ◽  
Author(s):  
A. A. Eryomenko ◽  
N. V. Rostunova ◽  
S. A. Budagyan ◽  
V. V. Stets
Keyword(s):  
Intensive Care ◽  
Intensive Care Units ◽  
Remote Monitoring ◽  
Clinical Testing ◽  
Transportation Of Patients ◽  
High Quality ◽  
Telemedicine System ◽  
Accuracy Of Measurements ◽  
Hospital Equipment

The experience of clinical testing of the personal telemedicine system ‘Obereg’ for remote monitoring of patients at the intensive care units of leading Russian clinics is described. The high quality of communication with the remote receiving devices of doctors, the accuracy of measurements, resistance to interference from various hospital equipment and the absence of its own impact on such equipment were confirmed. There are significant advantages compared to stationary patient monitors, in particular, for intra and out-of-hospital transportation of patients.

The visualization and assessment of the anterior surface of the cornea using video keratotopography

The Eye ◽  
2020 ◽  
Vol 22 (130) ◽  
pp. 36-43
Author(s):  
Gulnara Andrienko
Keyword(s):  
Contact Lenses ◽  
Corneal Topography ◽  
Topographic Maps ◽  
Specific Situation ◽  
Main Method ◽  
Pathological Conditions ◽  
Quality Of Vision ◽  
Corneal Shape ◽  
Color Map

Corneal topography is the main method for assessing the regularity of the surface of the cornea. Corneal irregularity leads to a deterioration in its refractive properties and a decrease in the quality of vision. Learning the basics of corneal topography will help determine the choice of a color map for a specific situation, as well as understand and analyze the data associated with these maps. This article describes the main types of topographic maps, various patterns of corneal shape in normal and pathological conditions and how to use the data obtained to design and fit contact lenses. Despite the fact that images may vary depending on topographers used, the information presented in this article is universal.

Measurement of cortical plate volume based on CBCT of frontal teeth in studies with retrusion and normality

2021 ◽  
pp. 17-21
Author(s):  
N. G. Meskhiya ◽  
I. S. Kopetskiy ◽  
I. A. Nikolskaya ◽  
D. A. Eremin ◽  
O. N. Kovaleva
Keyword(s):  
Orthodontic Treatment ◽  
Soft Tissues ◽  
Three Dimensions ◽  
Cone Beam ◽  
Cortical Plate ◽  
Imaging Method ◽  
New Approach ◽  
Accuracy Of Measurements ◽  
Bone Structures

Cone Beam Computed Tomography (CBCT) is the preferred imaging method for a comprehensive orthodontic examination. Thanks to the development of this technique, clinicians today can make most accurate measurements without fear of errors associated with projection distortion or localization of landmarks on radiographs. The quality of CBCT images gives to orthodontists the ability to analyze bone structures, teeth (even impacted teeth), and soft tissue in three dimensions. The accuracy of measurements of hard and soft tissues from CBCT images determines the accuracy of diagnosis and treatment planning. A fundamentally new approach has been proposed, which makes it possible to thoroughly study the bone tissue surrounding the tooth at the stages of planning orthodontic treatment. Аnalysis of radiation studies of patients with dentoalveolar anomalies was carried out to select the optimal treatment tactics and to control its effectiveness.

scholarly journals Structured, Analytical and Critical Thinking in the Educational Process of Future Teachers

2020 ◽  
Vol 10 (3) ◽  
pp. 111-118
Author(s):  
Pavel Beňo ◽  
Patrik Havan ◽  
Sandra Šprinková
Keyword(s):  
Critical Thinking ◽  
Creative Thinking ◽  
Educational Process ◽  
Point Of View ◽  
Problem Solving Skills ◽  
Main Method ◽  
Level Of Thinking ◽  
Access To Knowledge ◽  
The Right

AbstractIntroduction: In this article, we want to point out what kind of pedagogical and didactic change is being recorded in Slovakia’s education system and we will point out where it could go and develop to achieve positive results. This article is one of the upcoming outputs in the form of paper and study on the provision of structured, analytical and critical thinking (SAC). In the article, it is shown how the situation has changed and how we perceive the attitude of students during the educational process. Next, it is described current problems and inadequacies in the educational process and define how to use a change of thinking to increase motivation and improve access to knowledge.Purpose: In general, there is a consensus that it is important for teachers to be able to guide their students to problem-solving skills (Aktaş & Ünlü, 2013). It is pointed out that, with the right educational tools, such skills can be stimulated, developed and improved (Jordaan & Jordaan, 2005). This article is designed for all levels of education, but we are mostly concerned with educating future educators.Methods: In this paper, there are described methods that can help to improve the quality of thinking of students and thus increase the level of thinking of the whole society. This article take inspiration from important historical personalities as well as relevant current personalities in their professions. Critical, analytical and creative thinking, also based on logical and structured thinking, is our main method of our educational process.Conclusion: In conclusion, it is pointed out the need to develop SAC as a whole. It is important for the general publica to have better skills in SAC, for example, from the point of view of cognitive mistakes in experts, in the field of political literacy, recognition of misinformation and a better general awareness of rational thinking. As can be seen, SAC is not only about education, but it also closely affects society as a whole. It can thus influence the operation of the company, prevent the development of the first-class solutions offered and raise the whole company to a higher level.

Discuss the Vibration Frequency Reflection Wave Method of Low-Strain to Complete the Influence of Pile Inspection

2012 ◽  
Vol 170-173 ◽  
pp. 876-879
Author(s):  
Xue Qian Gu ◽  
Nan Gai Yi ◽  
Zhi Qiang Zhang
Keyword(s):  
Simulation Experiment ◽  
Excitation Frequency ◽  
Dynamic Test ◽  
Laboratory Simulation ◽  
Wave Method ◽  
Main Method ◽  
Integrity Testing ◽  
Foundation Pile ◽  
The Many

As the main method of the foundation pile low strain dynamic test, the reflected wave method has been widely recognized in engineering after years of research and application. Among the many elements of affecting quality of pile foundation, the influence of excitation frequency on pile inspection is research focuses in this paper. This paper analyzes and summarizes the rules of the effect the exciting force produces in low strain integrity testing by using laboratory simulation experiment.

scholarly journals Algorithms for Cytoplasm Segmentation of Fluorescence Labelled Cells

2002 ◽  
Vol 24 (2-3) ◽  
pp. 101-111 ◽  
Author(s):  
Carolina Wählby ◽  
Joakim Lindblad ◽  
Mikael Vondrus ◽  
Ewert Bengtsson ◽  
Lennart Björkesten
Keyword(s):  
Statistical Analysis ◽  
Single Cells ◽  
Quality Measurement ◽  
Feedback System ◽  
Cell Segmentation ◽  
New Combination ◽  
Processing Step ◽  
Segmentation Quality ◽  
Fully Automatic

Automatic cell segmentation has various applications in cytometry, and while the nucleus is often very distinct and easy to identify, the cytoplasm provides a lot more challenge. A new combination of image analysis algorithms for segmentation of cells imaged by fluorescence microscopy is presented. The algorithm consists of an image pre‐processing step, a general segmentation and merging step followed by a segmentation quality measurement. The quality measurement consists of a statistical analysis of a number of shape descriptive features. Objects that have features that differ to that of correctly segmented single cells can be further processed by a splitting step. By statistical analysis we therefore get a feedback system for separation of clustered cells. After the segmentation is completed, the quality of the final segmentation is evaluated. By training the algorithm on a representative set of training images, the algorithm is made fully automatic for subsequent images created under similar conditions. Automatic cytoplasm segmentation was tested on CHO‐cells stained with calcein. The fully automatic method showed between 89% and 97% correct segmentation as compared to manual segmentation.

Influence of Leaning Materials on the Quality of Building Ceramics

2021 ◽  
Vol 903 ◽  
pp. 197-202
Author(s):  
Ruta Švinka ◽  
Visvaldis Švinka ◽  
Maris Rundans ◽  
Inta Timma ◽  
Laila Petersone
Keyword(s):  
Thermal Expansion ◽  
Glass Fibre ◽  
Material Properties ◽  
Quartz Sand ◽  
Main Method ◽  
Different Types ◽  
Ceramic Building ◽  
Formation Of Cracks

Clay of the deposit Liepa is used for the production of ceramic building and finishing bricks in the factory “Lode” in Latvia. In the present work different types of these clays were investigated and “quartz effect” was determined using quartz sand as a leaning material. The substitution of quartz sand with milled E-glass fibre from Valmiera Glass was investigated and the changes in the magnitude of the quartz inversion effect were analysed. Thermal expansion was the main method for the determination of possible formation of cracks during technological process. Powder of milled glass fibre in amount of 5–10 % affects such properties as water uptake, porosity and apparent density and provides the required material properties at a lower firing temperature.

Irradiation of Food for Public Health Protection1

1982 ◽  
Vol 45 (4) ◽  
pp. 363-366 ◽  
Author(s):  
R. B. MAXCY
Keyword(s):  
Public Health ◽  
Food Additive ◽  
Subsequent Growth ◽  
Psychrotrophic Bacteria ◽  
Public Health Significance ◽  
Pure Cultures ◽  
Health Significance ◽  
Definition Of ◽  
Public Health Protection

Irradiation of food has a long history and has potential for improving public health protection, yet the technology is still not being used commercially. Public acceptance of the process has been hindered by fear and controversy, which involved erroneous definition of radiation as a food additive followed by some ill-conceived and ill-interpreted research. In general, all research has indicated radiation to be bactericidal with various degrees of effectiveness, depending on the specific bacteria being studied. The gram-negative psychrotrophic bacteria, as well as other Enterobacteriaceae, are quite sensitive to radiation and these bacteria are of particular interest to those persons responsible for protecting the quality of fresh red meat. Bacteria resisting less than sterilization doses in irradiation processes have been studied. Acquired increased resistance of bacteria through exposure survival, and subsequent growth of pure cultures can be obtained only through special laboratory manipulations. None of the presently available data indicate bacteria surviving irradiation are of special public health significance.

Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures.

1997 ◽  
Vol 63 (9) ◽  
pp. 3698-3702 ◽  
Author(s):  
L K Poulsen ◽  
H M Dalton ◽  
M L Angles ◽  
K C Marshall ◽  
S Molin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Simultaneous Determination ◽  
Single Cells ◽  
Pure Cultures

AFTER TREATMENT DOMESTIC SEWAGE WITH ERSHOVA LOADING

2012 ◽  
Vol 2 (2) ◽  
pp. 59-62
Author(s):  
Yu. V MURADYaN ◽  
S. Yu TEPLYKh
Keyword(s):  
Waste Water ◽  
Domestic Sewage ◽  
Technological Scheme ◽  
Treated Wastewater ◽  
Construction Technology ◽  
Wastewater Purification ◽  
Main Method ◽  
Water Fish

Widespread now technological scheme and construction technology of wastewater do not provide normative qualitative parameters that correspond to the requirements of the water fish-economical ponds.The limited financing of construction of new sewage is the main method of improving the reconstruction and/or intensification of existing sewage systems.Now for the intensification of the process of purification of biologically treated wastewater purification filter with the proposed design of the load which ershov fully satisfies modern requirements on quality of discharged waste water into a reservoir.

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