scholarly journals ANALYSIS OF APO-B SERUM LEVELS IN BALB/C MICE HYPERCHOLESTEROLEMIC AGAINST TEMULAWAK EXTRACT (Curcuma xanthoriza Roxb)

2019 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Riyadatus Solihah ◽  
M. Shofwan Haris ◽  
Yogi Khoirul Abror
Keyword(s):  
Treatment Group ◽  
Serum Levels ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
High Cholesterol ◽  
Ginger Extract ◽  
Control Group Design ◽  
Apo B

APO–B Serum levels is the most predictive value for the incidence of atherosclerosis and coronary heart disease. Curcuma xanthorrhiza Roxb contains curcumin, which can be used as an antioxidant, anti–inflammatory and antihypercholesterol. The mechanism of curcumin contained in ginger to reduce cholesterol is due to its function as a cholagoga or bile stimulant. This study aims to determine the effect of temulawak extract on the levels of APO–B Serum in hypercholesterolemia mice. This research were a true experimental study with a post–test only control group design carried out in February 2018. The extraction As much as 25 mice were divided into 5 groups where are group consisted of 5 mice. Positive control group (K+) were treated with high cholesterol feed and water, negative control group (K–) were given standard feed and water, treatment group 1 (P1) were given high cholesterol food and 25mg/kg BW of curcuma extract for 14 days, treatment group 2 (P2) were treated with foods high in cholesterol and 50mg/kg BW of curcuma extract for 14 days and treatment group 3 (P3) treated with high cholesterol and ginger extract 75mg/kg BW for 14 days. Examination of APO–B levels were measured using the spectrophotometric method. Data were analyzed using One–Way Anova. The results showed that the average of APO–B level at (K+) was 209.7 ± 1.02 mg / dL, at (K–) 115.3 ± 1.04 mg / dL, at (P1) 180.4 ± 1.07 mg / dL, at (P2) 147.6 ± 1.12 mg / dL, at (P3) 119.1 ± 1.10 mg / dL. Based on the results of statistical test it was found that there was a significant decrease in APO–B levels with p–value= 0.001 at alpha 0.05 (p <α).

scholarly journals Effect of Chitosan on Histology of Reproductive Organs of Female Wistar Rats (Rattus norvegicus) Exposed to Acetate Lead

2019 ◽  
Vol 30 (4) ◽  
pp. 259
Author(s):  
Alief Ayu Purwitasari ◽  
Annisa' Wigati Rozifa ◽  
Dwi Dianita Irawan ◽  
Umi Kalsum ◽  
Retty Ratnawati ◽  
...  
Keyword(s):  
Treatment Group ◽  
Lead Acetate ◽  
Antral Follicle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Lead is one of the pollutants widely spread in the environment because it is not easily decomposed. Lead can affect system functions such as the ovary and endometrium. Lead can trigger oxidative stress by reducing antioxidant enzymes and increasing Reactive Oxygen Species (ROS). Lead can also reduce Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels by disturbing the hypothalamus. Chitosan is an antioxidant compound that can reduce the toxic effects of lead. The purpose of this study was to study the effects of chitosan administration on the diameter of antral follicles, the number of endometrial arterioles, and the thickness of endometrial rats after lead acetate exposure. This study was an experimental laboratory using a posttest-only control group design approach applied on 25 female rats aged 8 weeks old, body weight 125-175 grams. Lead and chitosan were given orally with a sonde. There were 5 groups, namely, negative control group (without any treatment), positive control group (lead 175mg/kg/BW), treatment group 1 (lead 175mg/kg/BW + chitosan 16mg/kg/BW, treatment group 2 (lead 175mg/kg/BW + chitosan 32mg/kg/BW), and treatment group 3 (lead 175mg/kg/BW + chitosan 64mg/kg/BW) for 30 days. The rats were sacrificed at proestrus phase, which was proven from vaginal swab. Observations were carried out using the Hematoxylin Eosin (HE) staining method. The observations were analyzed using One Way ANOVA and followed by Least Significant Differences (LSD) test. The results showed significant results (p-value &lt;0.05). Chitosan can increase the diameter of the antral follicle, increase the number of endometrial arterioles, and increase the thickness of endometrial rats exposed by lead acetate.

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Effectiveness of Boiled Comfrey Leaves (Symphytum Officinale L) on SGOT SGPT Serum in Male Wistar Strain Rats With Acute Hepatitis Models

2019 ◽  
Vol 7 (1) ◽  
pp. 756-767
Author(s):  
Rois Sahidin ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Symphytum Officinale ◽  
Wistar Strain

Acute hepatitis is an acute disease caused by viruses, alcohol and drugs, which is characterized by an increase in SGOT SGPT serum. The purpose of this study was to determine the effectiveness of boiled comfrey leaves (Symphytum Officinale L) levels of SGOT and SGPT serum in male wistar strain rats with acute hepatitis models. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. The objects were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 2.8 grams of comfrey (symphytum officinale L) leaves was given as much as 2.7 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare serum levels of SGOT & SGPT. The results showed there were significant differences in serum levels of SGOT between the treatment group, positive control and negative control (p <0.05) and there were significant differences in SGPT results between the treatment group and positive control group (p <0.05) but there were no significant differences between the SGPT levels of the treatment group and the negative control group (p = 0.173). As Conclusion, boiled Comfrey leaf has an effect in decreasing serum SGOT & SGPT in male wistar strain rats with acute hepatitis model.

scholarly journals EFFECTIVENESS OF BOILED FIGS LEAF (FICUS CARICA L) TOWARDS UREUM AND CREATININ SERUM OF MALE WISTAR GALUR RATS On Acute Renal Failure Model

2019 ◽  
Vol 7 (1) ◽  
pp. 804-813
Author(s):  
Ralda Christina Ursula Pelealu ◽  
Untung Sudharmono
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Treatment Group ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group

Acute renal failure is a set of clinical symptoms characterized by increased levels of creatinine and blood urea nitrogen and a rapids decrease in the rate of glomerular filtration. The Objectives of this study was to determine the effectiveness of brewed tin water (Ficus Carica L) against ureum and creatinine serum of male Wistar strain rats in acute renal Failur. The subject of the research were 30 male rats weighing 180-200 grams and 2-3 months old, which were divided into 3 groups, namely the negative control group, the positive control group and the treatment group. Each group consisted of 10 rats. The negative control group was only given food and drink as usual. The positive control group and the treatment group were induced with gentamicin 12 mg / intraperitonial for 7 days to increase ureum and creatinine serum levels. The treatment group was given steeping treatment of 0.65 grams of boiled figs leaf in 100cc of given 3.6cc/orally for 7 days. One way ANOVA test was conducted to analyze the study using SPSS version 24. The results found there are no significant differences in ureum serum levels between the treatment group and negative control group (p = 0.252) but there were significant differences in the ureum serum value between the treatment group and positive control group (p <0.05). There was a significant difference in the value of creatinine serum between the treatment group, the positive control group and the negative control group (p <0.05). As Conclusions boiled figs leaf (Ficus Carica L) has an effect on decreasing ureum and creatinine serum levels in male wistar rats with acute renal failure..

scholarly journals POTENTIAL ETHANOL OF Carica papaya L. EXTRACT AS IMMUNOMODULATORY THROUGH HISTOLOGY OBSERVATION AT MICE BALB/C SPLEEN

2017 ◽  
Vol 13 (2) ◽  
pp. 205
Author(s):  
Sri Tasminatun ◽  
Ria Pravitasari ◽  
SN. Nurul Makiyah
Keyword(s):  
Treatment Group ◽  
Carica Papaya ◽  
Negative Control Group ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
One Way Anova

Abstract: The diameter of the alba pulp of the spleen will be enlarged, when the body responds to allergens by increasing the activity of the immune system. Papaya fruits (Carica papaya L.) containing flavonoid that can be as an immunomodulator. This study aims to determine the effect of C. papaya extract to the diameter size alba pulp of the spleen of Ovalbumin (OVA) induced Balb/C mice. The research design was experimental in vivo animal test with post-test only control group design. Thirty male Balb/C mice, ±20grams weight, 8 weeks old, divided into six groups consists of a normal control, negative control, Methylprednisolone, and C. papaya extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day). The extract and Methylprednisolone are given for 28 days, while OVA is given on 15thand 22ndday (intraperitoneal,) and 23rd until 28thday (peroral). On the 29thday the mice are sacrificed and the duodenum was made into histological preparation with HE staining. The immunomodulator effect is assessed by the average of the diameter size alba pulp of the spleen under a light microscope. The data were analyzed using SPSS15 with one way ANOVA continued with Tukey test. The average of the diameter size alba pulp of the spleen for control group is 10,165 ± 0,195; negative control group is 30,357 ± 0,826; methylprednisolone group is 10,220 ± 0,346; 175mg/kgbb dose extract is 14,956 ± 1,057; 350mg/kgbb dose extract is 14,030 ± 0,667; and 700mg/kgbb dose extract is 12,504 ± 0,729. The conclusion of this study is Carica papaya L. extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day) has an effect to the average of the diameter size alba pulp of the spleen in OVA-induced Balb/C mice significantly. 700mg/kg dose per day has the same effect with the methylprednisolone group. Keywords: immunomodulator, Carica papaya L., alba pulp of the spleen, ovalbumin

scholarly journals Moderate Intensity Exercise is Most Influential in The Expression of UCP-1 in Mice Male

2019 ◽  
Vol 3 (12) ◽  
pp. 490-492
Author(s):  
Chabib Fachry Albab ◽  
Purwo Sri Rejeki ◽  
Muhammad Miftahussurur ◽  
Soebagijo Adi Soelistijo
Keyword(s):  
Treatment Group ◽  
Exercise Intensity ◽  
Intensity Variation ◽  
Negative Control Group ◽  
Negative Control ◽  
Moderate Intensity ◽  
Control Group ◽  
Male Mice ◽  
Moderate Intensity Exercise ◽  
Control Group Design

Sufficient exercise was considered enough to reduce the number of obesities through the process of fat browning. This research was conducted to prove the effect of exercise intensity on fat browning process through increased UCP-1 expression in male mice. Laboratory experimental research with a post test only control group design approach using 24 mice was divided into 4 groups. Determination of the group was based on the negative control group, mild intensity swimming treatment group, moderate intensity swimming treatment group, and heavy intensity swimming treatment group. The treatment was carried out for four weeks with a frequency of three times a week. The control group had UCP-1 expression of 5.47 ± 0.83 IRS / LP, the mild intensity swimming group had UCP-1 expression of 7.23 ± 1.57 IRS / LP, the moderate intensity swimming group had UCP-1 expression of 9.17 ± 1.65 IRS / LP, and the heavy intensity swimming group had an expression of 6.93 ± 1.42 IRS / LP. The giving of exercise intensity variation affects the expression of UCP-1 in male mice. Moderate intensity exercise has the highest UCP-1 expression. Keywords: exercise intensity; UCP-1; male mice

scholarly journals The Effect of Hydrogel Aloe vera (Aloe vera (L.) Burm) on the Number of Neutrophil Cells in Aggressive Periodontitis Induced by Aggregatibacter actinomycetemcomitans (In Vivo Study on Wistar Rats)

2021 ◽  
Vol 5 (7) ◽  
pp. 657-663
Author(s):  
Chandra Susanto ◽  
Shieny Lokanata ◽  
Jeni Widya Ningrum
Keyword(s):  
Wistar Rats ◽  
Alveolar Bone ◽  
Aloe Vera ◽  
Negative Control Group ◽  
Aggressive Periodontitis ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Introduction: Aggressive periodontitis is a periodontal disease that runs rapidly, characterized by gross alveolar bone loss, the disease is unprompted by complaints, not related to local aspects. Objective: The aim of this study was to determine the effect of Aloe vera hydrogel on the number of neutrophil cells in aggressive periodontitis induced by A. actinomycetemcomitans in Wistar rats. Methods: This type of research is a laboratory experimental design with post-test only design with control group design. This study used 24 Wistar rats divided into 6 groups, namely, negative control group (Basic hydrogel), ibuprofen and hydrogel Aloe vera 2.5%, 5%, 10%, 20%. Results: The test results for the mean number of neutrophil cells in the hydrogel aloe vera group of 2.5%, 5%, 10%, 20%, ibuprofen, and negative controls were 120.50 ± 12.92, 78.25 ± 7.37, 47, 25 ± 4.03, 1.00 ± 0.82, 0.50 ± 0.58, and 217.25 ± 62.26. The results of the oneway Anova statistical test obtained p value = 0.001, which means that there is a difference in the number of neutrophils of Wistar rats in all treatment groups. Conclusion: The conclusion of this study there is the effect of giving hydrogel aloe vera a concentration of 2.5%, 5%, 10%, 20% with ibuprofen on Wistar rats induced by aggressive periodontitis.

scholarly journals Perbedaan Efektivitas Ekstrak Jahe Dengan Ekstrak Kunyit Dalam Mengurangi Nyeri Dismenorhea Primer Pada Mahasiswi Di Asrama Jurusan Kebidanan Poltekkes Surakarta

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Gita Kostania ◽  
Anik Kurniawati
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Control Group ◽  
P Value ◽  
Ginger Extract ◽  
Control Group Design ◽  
Department Of Health ◽  
Turmeric Extract ◽  
Primary Dysmenorrhoea ◽  
Post Test

Abstract: Dysmenorrhoea, Ginger, Turmeric. The aim of this study was to determine differences in the effectiveness of ginger extract with turmeric extract on reducing pain in primary dysmenorrhoea on dormitory student at the Midwifery Department of Health Polytechnic of Surakarta. The design of this research is experimental design, with a truly experimental approach, using a pretest-post test control group design. Criteria sampling is purposive random sampling, a total sample of 60 respondents (55% of the population, nomogram Harry King Samples), divided into three equal groups: ginger extract treatment group, turmeric extract treatment group, and placebo group. Each group consists of 20 respondents. Results: the data was in a normal distribution, the total number of respondents at the end of the study was 56 respondents (ginger extract treatment=19, turmeric extract treatment= 19, and placebo=18). There are differences in the effectiveness of ginger extract with turmeric extract in reducing pain in primary dysmenorrhoea on dormitory student at the Midwifery Department of Health Polytechnic of Surakarta (p-value=0.04 <0.05). Extracts of ginger are more effective in reducing menstrual pain than turmeric extract (t-test = 9.690> 4.802).

scholarly journals The Effect of Hydrogel Aloe vera (Aloe vera (L.) Burm) on the Number of Neutrophil Cells in Aggressive Periodontitis Induced by Aggregatibacter actinomycetemcomitans (In Vivo Study on Wistar Rats)

2021 ◽  
Vol 5 (3) ◽  
pp. 685-691
Author(s):  
Chandra Susanto ◽  
Shieny Lokanata ◽  
Jeni Widya Ningrum
Keyword(s):  
Wistar Rats ◽  
Alveolar Bone ◽  
Aloe Vera ◽  
Negative Control Group ◽  
Aggressive Periodontitis ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Introduction: Aggressive periodontitis is a periodontal disease that runs rapidly, characterized by gross alveolar bone loss, the disease is unprompted by complaints, not related to local aspects. Objective: The aim of this study was to determine the effect of Aloe vera hydrogel on the number of neutrophil cells in aggressive periodontitis induced by A. actinomycetemcomitans in Wistar rats. Methods: This type of research is a laboratory experimental design with post-test only design with control group design. This study used 24 Wistar rats divided into 6 groups, namely, negative control group (Basic hydrogel), ibuprofen and hydrogel Aloe vera 2.5%, 5%, 10%, 20%. Results: The test results for the mean number of neutrophil cells in the hydrogel aloe vera group of 2.5%, 5%, 10%, 20%, ibuprofen, and negative controls were 120.50 ± 12.92, 78.25 ± 7.37, 47, 25 ± 4.03, 1.00 ± 0.82, 0.50 ± 0.58, and 217.25 ± 62.26. The results of the oneway Anova statistical test obtained p value = 0.001, which means that there is a difference in the number of neutrophils of Wistar rats in all treatment groups. Conclusion: The conclusion of this study there is the effect of giving hydrogel aloe vera a concentration of 2.5%, 5%, 10%, 20% with ibuprofen on Wistar rats induced by aggressive periodontitis.

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