scholarly journals Survival and viability of Bradyrhizobium japonicum in different liquid medium

2021 ◽  
Vol 82 (5-6) ◽  
pp. 245-255
Author(s):  
Gabriella Kanižai Šarić ◽  
Ivana Majić ◽  
Krešimir Prtenjača
Keyword(s):  
Liquid Medium ◽  
Nutrient Medium ◽  
Bradyrhizobium Japonicum ◽  
Storage Time ◽  
Optimal Composition ◽  
Viable Cells ◽  
Traditionally Prepared ◽  
Two Temperatures ◽  
Time Periods ◽  
Glycerol Medium

The microbiological inoculants present on the market come in various formulations and forms. Inoculants used in pre-sowing bacterization of legumes in our country are traditionally prepared on peat as the highest quality carrier of bacteria. However, the requirements of the manufacturer are focused on liquid forms of inoculants. Therefore, the aim of this paper is to determine the optimal composition of the liquid medium that will support the growth of Bradyrhizobium japonicum, the soybean symbiont. Three liquid nutrient medium formulations were included in the study: mannitol-yeast medium, modified mannitol-yeast medium, and glycerol medium stored at two temperatures (4 and 25 °C), and B. japonicum cell viability was determined over 6 months. The results showed that the largest number of rhizobia (on average 4x109 zo 9x108) was obtained on yeast-mannitol medium at 4 °C as well as on modified yeast mannitol medium where their number remained constant throughout the storage time and was 5x107 cfu ml-1 at 25 °C. Further research should include testing other rhizobial protectors in order to increase the number of viable cells in longer time periods.

scholarly journals Storage Time and Temperature on the Sensory Properties Broccoli

Foods ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 162 ◽  
Author(s):  
Robert Pellegrino ◽  
Jennifer Wheeler ◽  
Carl E. Sams ◽  
Curtis R. Luckett
Keyword(s):  
Storage Time ◽  
Storage Temperature ◽  
Consumer Acceptance ◽  
Biochemical Processes ◽  
Time Points ◽  
Quality Degradation ◽  
Two Temperatures ◽  
Sensory Changes ◽  
Acceptance Tests ◽  
Holding Temperature

Typically, broccoli arrives at the store within 7–14 days of harvest and is kept refrigerated until purchased or considered waste. To date, information has been limited on how this time on the shelf or storage temperature affects the sensory attributes that contribute to broccoli purchase or repurchase. In this study, 100 consumers performed acceptance tests and a check-all-that-apply (CATA) section to characterize sensory changes in two cultivars of broccoli (‘Diplomat’ and ‘Emerald Crown’) stored at two temperatures (0 °C and 4 °C) over five time points: 0, 14, 21, 28, and 42 days. Due to quality degradation during storage, the overall liking of broccoli decreased regardless of holding temperature and variety. This was in accordance with a decrease in sweetness and an increase in bitterness intensity. However, there were differences between varieties in which Diplomat had more sensory changes at higher temperatures and only Emerald showed negative changes to its appearance in color. Lastly, the CATA data revealed the attributes responsible for modulating the consumer acceptance of broccoli such as tastes, colors and flavors (e.g., grassy, musty, dirt-like). This information can be used to better inform shelf-life determinations of broccoli. Additionally, these changes in taste, odor, texture, and color can inform those interested in investigating the biochemical processes related to broccoli storage.

Metabolism of the Plant Growth Regulator (E)-[3H]2-Ethylhex-2-enoic Acid in Hordeum vulgare

1986 ◽  
Vol 41 (1-2) ◽  
pp. 141-147 ◽  
Author(s):  
Bernd Schneider ◽  
Horst-Robert Schütte ◽  
Alfred Preiss
Keyword(s):  
Nmr Spectroscopy ◽  
Hordeum Vulgare ◽  
Plant Growth ◽  
Growth Regulator ◽  
Nutrient Medium ◽  
Plant Growth Regulator ◽  
Time Course ◽  
Enoic Acid ◽  
H Nmr ◽  
Time Periods

The metabolism of (E)-[3H]2-ethylhex-2-enoic acid (EHA) was studied in excised seedlings of barley (Hordeum vulgare). It was rapidly taken up from the nutrient medium. The metabolites, isolated by extraction with methanol, separated and purified by TLC and HPLC, were identified by enzymatic, chemical, and spectrometric methods, especially 1H-NMR spectroscopy. The time course of metabolism during 6 , 12, 24, 48, and 72 h is presented, indicating interconversation reactions. A rapid conjugation with glucose was observed, decreasing in concentration again after longer time periods in favour of disaccharide esters, higher conjugates, and a hydroxylation product which was present in free and conjugated form.

Viability of an ectomycorrhizal inoculum produced in a liquid medium and entrapped in a calcium alginate gel

1987 ◽  
Vol 65 (11) ◽  
pp. 2326-2329 ◽  
Author(s):  
Ch. Mauperin ◽  
F. Mortier ◽  
J. Garbaye ◽  
F. Le Tacon ◽  
G. Carr
Keyword(s):  
Liquid Medium ◽  
Calcium Alginate ◽  
Storage Time ◽  
Storage Conditions ◽  
Soil Extract ◽  
Sterile Soil ◽  
Soil Extracts ◽  
Alginate Gel ◽  
Calcium Alginate Gel ◽  
Soil Hyphae

Survival of Hebeloma crustuliniforme (Bulliard ex Fries) Quelet, grown in a liquid medium and subsequently entrapped in beads of calcium alginate, was tested under different conditions. Mycelium viability was not affected either by curing the beads for up to 22 h in 0.7 M CaCl2 or by the addition of either peat or bentonite to the alginate gel. Both peat and bentonite improved the water retention of the alginate gel, but only the incorporation of bentonite slowed down the rate at which moisture was lost by evaporation. At 4 °C the entrapped mycelium retained its viability for at least 5 months, provided that storage conditions remained humid. Partial drying of the beads reduced the effective storage time to a month. Emergence of hyphae from the beads was influenced by the presence of sterile soil extracts prepared from a podzolic soil, an acid brown earth, a mesotrophic brown earth, an eutrophic brown earth, and a rendzina. Hyphae grew out of all the beads containing peat irrespective of the type of soil extract but only grew out of those containing bentonite when they were on the mesotrophic brown earth extract. Hyphal emergence from beads containing neither peat nor bentonite appeared to be influenced by the pH of the extract, being better on those that were more acidic. Both sterile and nonsterile nursery soil supported growth of hyphae from the three types of bead, but on nonsterile soil, hyphae were not abundant and those that developed began to die off sooner than those on sterile soil.

Effects of Storage and Service on Tire Performance: Oil Component Content and Swelling Behavior

2003 ◽  
Vol 76 (2) ◽  
pp. 507-516 ◽  
Author(s):  
Takahiro Kataoka ◽  
Per B. Zetterlund ◽  
Bunchiro Yamada
Keyword(s):  
Tensile Strength ◽  
Storage Time ◽  
Rate Of Change ◽  
Swelling Behavior ◽  
Passenger Car ◽  
Elongation At Break ◽  
Component Content ◽  
Time Periods ◽  
Oil Components ◽  
Tread Rubber

Abstract In order to investigate the effects of storage and service on passenger car tire performance, two separate sets of samples were characterized: (i) tires stored at a temperature of 45–50 °C for different time periods, and (ii) used tires of different mileage. The tires were analyzed with respect to the performance of the tread rubber as manifested in the modulus at 200% elongation, the tensile strength at break, the elongation at break, the hardness, the viscoelasticity of the rubber, swelling, and the oil component content. The change in the properties of the tires as functions of increasing storage time and mileage indicated a marked deterioration in performance. The rate of change in these properties was found to be markedly enhanced by the extra stimuli provided by service, as demonstrated by comparing tires of similar age but different mileage. The results were analyzed and rationalized in terms of the change in the degree of crosslinking and transfer of oil components from the tread rubber to the inner part of the tire or to the surroundings.

Influence of storage time and nutrient medium on recovery of fibroblast-like cells from refrigerated collared peccary (Pecari tajacu Linnaeus, 1758) skin

2018 ◽  
Vol 54 (7) ◽  
pp. 486-495 ◽  
Author(s):  
Luiza Bento de Queiroz Neta ◽  
Gabriela Pereira de Oliveira Lira ◽  
Alana Azevedo Borges ◽  
Maria Valéria de Oliveira Santos ◽  
Maria Bárbara Silva ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Storage Time ◽  
Pecari Tajacu ◽  
Collared Peccary

scholarly journals Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods

2017 ◽  
Vol 71 (2) ◽  
pp. 98-106
Author(s):  
Slobodanka Vakanjac ◽  
Vladimir Magas ◽  
Vladan Babic ◽  
Milan Maletic ◽  
Spomenka Djuric ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Storage Time ◽  
Intrauterine Insemination ◽  
Routine Practice ◽  
Reproductive Parameters ◽  
Swine Industry ◽  
Newborn Piglets ◽  
Time Periods ◽  
Semen Extender

Introduction. Artificial insemination of sows with cooled semen has become a routine practice in the swine industry and has almost completely ruled out natural mating. The storage time of the cooled semen and preservation of its qualitative parameters are of the upmost importance and depend partly on the semen extender used. The aim of this study was to determine the quality of cooled semen during storage in a thermobox with a commercial extender by assessment of sperm motility, cytomorphology, and assessment of reproductive parameters in sows after intrauterine insemination. Materials and Methods. The semen was preserved with Duragen? (Magapor, Spain) extender and stored at 17?1?C. A total of 110 sows were included in the study and randomly divided into four groups: K1 (n=25) , K2 (n=25), K3 (n=30), and K4 (n=30). The sows were inseminated twice with semen (>1x107/ml spermatozoa) which had been stored for 1 day (S1), 3 days (S3), 5 days (S5), or 7 days (S7). Pregnancy was diagnosed by imaging ultrasound. Results and Conclusions. The best results were obtained when the sows were inseminated with semen stored for one day. However, there were no statistically significant differences in the number and vitality of newborn piglets when semen stored for 3 or 5 days was used. Intrauterine insemination resulted in a satisfactory number of farrowings and piglets despite the fact that a relatively low number of spermatozoa was determined in each dose used. The use of semen with a higher dilution rate and its intrauterine application ensured a larger number of obtained doses from one ejaculate and indicates this would lead to a more profitable use of boars.

scholarly journals Evaluation of optimal storage time of tuberculous lesions stored in sodium borate

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3685 ◽  
Author(s):  
Flávia Morato ◽  
Cássia Yumi Ikuta ◽  
Amane Paldês Gonçales ◽  
Gisele Oliveira de Souza ◽  
Marcos Amaku ◽  
...  
Keyword(s):  
Storage Time ◽  
Environmental Temperature ◽  
Sodium Borate ◽  
Control Group ◽  
Large Country ◽  
Tissue Samples ◽  
Progressive Decline ◽  
Tuberculous Lesion ◽  
Two Temperatures ◽  
Step Study

Preservation of specimens during transportation between abattoirs and diagnostic laboratories defines a critical stage in the definitive diagnosis of bovine tuberculosis by the isolation of Mycobacterium bovis. A 2-step study was designed to verify the maximum time that tissue samples can be stored in saturated sodium borate solution (SSB) with the highest detection of M. bovis isolates. Ninety hamsters were inoculated intraperitoneally with a suspension of M. bovis strain AN5 and were humanely euthanized after 40 days. Their spleens were collected and stored in SSB during four distinct periods (30, 60, 90 and 120 days) with incubation at two temperatures (27°C and 37°C). The control group was cultured on the day of euthanasia. Sixty-nine suspected tuberculous lesions samples were collected in the abattoir and were stored in SSB for three periods (30, 60 and 90 days) at 27°C in the laboratory. The bovine control group was cultured on the day of entry in the laboratory. Both experiments were analyzed separately based on the growth proportion of isolates and the number of colonies. SSB was able to maintain the viability of most M. bovis at high temperatures for up to 30 days. A progressive decline was observed with other storage periods at 27°C, and no growth was obtained after 60-day storage at 37°C. Despite the loss in viability of M. bovis, SSB is the most favorable choice to preserve specimens during transportation across a large country with high variation in environmental temperature. The sensitivity of M. bovis detection by bacteriological examination is inversely proportional to storage time. Therefore, the storage of tuberculous lesion specimens in SSB is recommended to not exceed 30 days at 27°C before cultivation.

STRAIN SELECTION BRADYRHIZOBIUM JAPONICUM AND SINORHIZOBIUM FREDII TO RESISTANT TO AMMONIUM MOLYBDATE IN GROWING MEDIUM

1970 ◽  
pp. 53-56
Author(s):  
М. V. Yakimenko ◽  
S. А. Begun ◽  
А. I. Sorokina
Keyword(s):  
Nutrient Medium ◽  
Bradyrhizobium Japonicum ◽  
Ammonium Molybdate ◽  
Good Growth ◽  
Sinorhizobium Fredii ◽  
Poor Growth ◽  
Moderate Growth ◽  
Growth Intensity ◽  
Pure Cultures ◽  
Bacterial Mass

The article presents the results of the study of the resistance of Bradyrhizobium japonicum (Jordan, 1982) and Sinorhizobium fredii (Scholla, Elkan, 1984) strains, selection of the FSBSI ARSRI of Soybean, to ammonium molybdate in a nutrient medium. In the variant without introducing a molybdenum salt, from 47 strains of rhizobia of the B. japonicum species, 37 strains showed good growth, 3 strains-abundant and 7 strains-moderate growth of the bacterial mass stroke. When 1 g/l of ammonium molybdate was introduced into the nutrient medium, the growth intensity of the bacterial mass stroke of all tested B. japonicum decreased slightly; the number of moderately growing strains increased to 11, and one poorly growing strain appeared. When 10 g/l of ammonium molybdate was introduced into the nutrient medium, the strains of B. japonicum, showing abundant and good growth of bacterial mass, were not detected. 31 strains of rhizobia of this species have stopped their growth. In the variants with a nutrient medium MRS without a molybdenum salt and with 1 g/l molybdenum salt from 44 strains of rhizobia of the species S. fredii 93-95 % of pure cultures showed abundant and good bacterial mass stroke growth. When 10 g/l of ammonium molybdate was introduced into the nutrient medium, no rhizobia strains of the S. fredii species were found with abundant and good growth of the bacterial mass, 12 rhizobia strains of this species showed poor growth, and 4 strains stopped growing at this concentration of molybdenum salt in the nutrient environment. It was established that ammonium molybdate at a concentration of 1 g/l in a nutrient medium, practically does not affect the growth intensity of the bacterial mass stroke of strains B. japonicum and S. fredii.

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