scholarly journals Assessment of Microbial Load on Fresh-Cut Pineapple in Koforidua (Ghana) Market

2019 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Escherichia Coli ◽  
Microbial Contamination ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Microbial Count ◽  
Plate Method ◽  
Colony Forming Units ◽  
Fresh Cut ◽  
Growth Of Bacteria

Fresh-cut fruits are fruits that have been peeled, trimmed, chopped and packaged for consumers while still maintaining its freshness. Fresh-cut fruits are increasingly becoming popular with consumers because of its convenience. A major challenge faced in Ghana is the inappropriate method of processing to maintain the quality of the fresh-cut fruits. This may increase the incidence of microbial contamination which will then reduce the shelf-life of fresh-cut fruits as well as causing foodborne illness to consumers. This study was therefore conducted to assess the microbial loads on fresh-cut pineapple sold in Koforidua market in Ghana. Fresh-cut pineapples were obtained from three different vendors in Koforidua central market in Ghana. Pour plate method was used to analyze five microorganisms which include Total coliform bacterial, Escherichia coli, Salmonella, Yeast and moulds on the fresh-cut pineapple samples. The microbial counts were transformed into colony forming units (cfu) and analyzed using SPSS. Salmonella plates showed no growth of bacteria colonies, but total coliform bacteria, Escherichia coli, yeast and moulds showed growth on the pour plates. The analyses indicated that the microbial count among vendors were not significantly different (P>0.05). The total coliform count showed a range of 1.1×103 to 1.2×103 cfu, 1.0 ×103 cfu for Escherichia coli, yeast ranged from 1.2×103 to 4.7×103 cfu, moulds ranged from 0 to 2.5×103cfu forall the three vendors. All the values indicated for the microorganisms analyzed are above the acceptable limit of microorganisms in food. It is therefore concluded that fresh-cut fruits sold on koforidua market are not quality for consumption; however vendors need to be trained on proper conditioning of the fruits prior to consumption.

Microbial Quality of Bagged Baby Spinach and Romaine Lettuce: Effects of Top versus Bottom Sampling

2012 ◽  
Vol 75 (1) ◽  
pp. 132-136 ◽  
Author(s):  
JULIE A. KASE ◽  
STACEY BORENSTEIN ◽  
ROBERT J. BLODGETT ◽  
PETER C. H. FENG
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Microbial Quality ◽  
Romaine Lettuce ◽  
Probable Number ◽  
E Coli ◽  
Bottom Sampling

Contamination with Escherichia coli O157:H7 and Salmonella have called into question the safety and microbial quality of bagged ready-to-eat leafy greens. This study expands on previous findings that these goods have high total bacteria counts (TBC) and coliform counts, variation in counts among different lots, that Escherichia coli is present, and disparities in counts when bags are top or bottom sampled. Nearly 100 bags of baby spinach and hearts of romaine lettuce from a single brand were subjected to both top and bottom sampling. Product was blended, and a portion serially diluted and plated to obtain TBC. Total coliform and E. coli levels were estimated by the most-probable-number (MPN) technique with ColiComplete discs. Top-sampled TBC from bags of baby spinach (48 bags, 13 different lots) ranged from 3.9 to 8.1 log CFU/g and bottom-sampled TBC ranged from 4.0 to 8.2 log CFU/g, with 52% of the bags (or 39% of the lots) producing TBC higher in bottom samples. For hearts of romaine (47 bags from 19 different lots), top-sampled bags had TBC ranging from 2.4 to 7.0 log, and bottom-sampled bags had TBC from 3.3 to 7.3 log, with 64% of the bags (or 63% of the lots) showing higher TBC in bottom samples. However, we are unable to reject the hypothesis that the top and bottom samples from either commodity contain the same TBC (P ≥ 0.08). No E. coli was detected and total coliform bacteria counts were, with few exceptions, ≥210 MPN/g, irrespective of TBC. In general, lots with the most number of days before the printed “use-by” date had lower TBC. However, the R2 values for either baby spinach (0.4085) or hearts of romaine (0.2946) suggest that age might not be a very good predictor of higher TBC. TBC varied widely between lots and even more so within same-lot samples, as indicated by the sum of squares results. This finding, along with higher TBC in bottom samples, suggests further consideration when a microbiological sampling scheme of bagged produce is designed.

scholarly journals Identifikasi Bakteri Escherichia Coli pada Air Minum Isi Ulang yang Diproduksi Depot Air Minum Isi Ulang di Kecamatan Padang Selatan

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Fathoni Afif ◽  
Erly Erly ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
Microbiological Parameters ◽  
Complete Test ◽  
Biochemistry Test

AbstrakMenurut Permenkes No. 492 tahun 2010, air minum berkualitas dinilai dari parameter mikrobiologi adalah tidak ditemukannya bakteri total coliform dan Escherichia coli dalam 100 ml sampel. Menurut beberapa penelitian sebelumnya, banyak depot air minum isi ulang yang memproduksi air tidak sesuai dengan persyaratan secara mikrobiologi tersebut. Tujuan penelitian ini adalah untuk mengidentifikasi kehadiran bakteri Escherichia coli pada air minum yang diproduksi depot air minum isi ulang di Kecamatan Padang Selatan. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas pada sampel air yang didapat dari 13 depot yang tidak melakukan uji kualitas. Jenis penelitian ini adalah deskriptif. Data diperoleh dengan cara observasi dan wawancara langsung. Penelitian mikrobiologi air menggunakan metode Most Probable Number (MPN) yang terdiri dari presumptive test dengan menggunakan media Lactose Broth (LB) dengan metode 3 tabung, confirmative test dengan menggunakan media Brillian Green Lactose Broth (BGLB), complete test dengan menggunakan media endo agar, dan tes identifikasi dengan menggunakan media uji biokimia. Hasil penelitian didapatkan bahwa 10 dari 13 sampel tercemar oleh bakteri coliform dan 2 sampel memenuhi syarat untuk penelitian selanjutnya secara kualitatif. Pada penelitian lanjutan, tidak ditemukan bakteri Escherichia coli namun ditemukan bakteri Pseudomonas aeruginosa.Kata kunci: escherichia coli, depot air minum isi ulang, MPN AbstractAccording to the Minister Regulation. 492 in 2010, assessed quality of drinking water in microbiological parameters is by the discovery of total coliform bacteria and Escherichia coli in a 100ml sample. According to some previous studies, many drinking water that produced by the depot does not conform to the microbiological requirements The objective of this study was to identify the presence of the Escherichia coli in drinking water produced by refill drinking water depot in the District of South Padang. Research had been carried out in the Laboratory of Microbiology, Faculty of Medicine, University of Andalas in water samples obtained from 13 depots that don’t test the quality. This research was a descriptive. Data obtained by direct observation and interviews. This research used Most Probable Number (MPN) test for the microbiological of the water that consist of presumptive test using Lactose Broth (LB), confirmative test using Brilliant Green Lactose Broth (BGLB), complete test using endo jelly media, and identification test using biochemistry test. The results showed that 10 of 13 samples contaminated by coliform bacteria and 2 samples eligible for further research that show there is a Pseudomonas aeruginosa.Keywords: escherichia coli, refill water depot, MPN

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from <3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

scholarly journals Analysis of Water Quality and River Waters Microbology for Manifestation of Food Safety

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Rizky Muliani Dwi Ujianti ◽  
Althesa Androva
Keyword(s):  
Water Quality ◽  
Food Security ◽  
Quality Management ◽  
Water Quality Management ◽  
Quality Standard ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Domestic Waste ◽  
Total Coliform Bacteria

 Abstract. Banjir Kanal Barat is a river in the Garang watershed, Semarang City, Central Java, Indonesia. Its function is as a source of water for the community. The level of pollution in this river is already high. The purpose of this study is to provide advice to governments, communities and related stakeholders to realize integrated river management, and fisheries-based food security is achieved. This research method is: analyzing the water quality of the Banjir Kanal Barat river, and analyzing the amount of faecal and total coliform bacteria content in the Banjir Kanal Barat river, and analyzing how to overcome the decline in the quality of waters of the Banjir Kanal Barat river due to faecal and total coliform bacteria pollution. The results showed that the water quality at the research location was still in the quality standard. The content of coliform dan faecal bacteria at the study site exceeds the quality standard, this is due to the influence of domestic waste from households. The thing that needs to be done is counseling the existence of a clean and healthy life, especially for people who are still throwing domestic waste into the river. The existence of water purification equipment is also very necessary to overcome this problem. Water quality management can be done with policy analysis. Regulations related to water quality management can be analyzed and then given solutions and recommendations related to these rules so that policies can be taken that are sustainable, integrated, and coordinated between various parties in managing river water quality and food security. Keywords: food security, water quality, river, faecal coliform, total coliform

scholarly journals Bacterial Contamination of Street Vended Food Pani Puri Available in Janakpurdham, Dhanusha

2019 ◽  
Vol 6 ◽  
pp. 70-75
Author(s):  
Nagendra Prasad Yadav ◽  
Rakesh Kumar Yadav ◽  
Satyam Prakash
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Food Samples ◽  
Microbial Count ◽  
Microbiology Laboratory ◽  
Dilution Technique ◽  
Bacillus Spp ◽  
Klebsiella Spp

Objectives: To determine the level of bacterial contamination in food (Panipuri) available in market of Janakpur, Dhanusha, Nepal. Methods: Total 120 samples of Panipuri (60 solid matters and 60 masalaa Pani) collected aseptically from the market were transported to the microbiology laboratory and processed for microbial count by serial dilution technique. Microbial load and presence of pathogen were detected in each sample. Results: Analysis of the food samples revealed that 70% of Panipuri samples had high loads of bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella spp, Pseudomonas spp, Bacillus spp.  Conclusion: This study depicted that the street vended food Panipuri of Janakpur are highly contaminated with pathogenic bacteria which can cause health risk to consumers. Thus, it is suggested that regular monitoring of the quality of street foods must be practiced to avoid any food bone illness in future.

scholarly journals Staphylococcus aureus and Escherichia coli in Curd Cheese Sold in the Northeastern Region of South America

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Tamiles Barreto de Deus ◽  
Ludmilla Santana Soares Barros ◽  
Ricardo Mendes da Silva ◽  
Wanessa Karine da Silva Lima ◽  
Danuza das Virgens Lima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
South America ◽  
Total Coliform ◽  
E Coli ◽  
Mold And Yeast ◽  
Sanitary Conditions ◽  
Microbiological Analyses ◽  
Microbiological Data

The present study evaluated the microbiological and sanitary quality of curd cheese sold on the beaches of the Itaparica Island, Brazil, and verified whether a correlation exists between the commercialization conditions and the microbiological data. The research was performed between December 2015 and March 2017. Sixty samples of rennet-containing cheese were collected to estimate the populations of mesophylls, psychrotrophic microorganisms, mold and yeast, Staphylococcus aureus, total coliforms, and Escherichia coli. An observational analysis was performed during the collection, using a checklist to verify the sellers’ sanitary conditions and cheese marketing. A high nonconformity index was registered regarding aspects in the checklist. In the microbiological analyses, the number of mesophylls in raw and roasted samples ranged from 7,88 to 14,82 log CFU/mL, and those of psychrotrophs ranged from 2,80 to 3,84 log CFU/mL. Meanwhile, mold and yeast levels in the samples ranged from 8,06 to 5,54 log CFU/mL, S. aureus was detected at levels from 3,24 to 4,94 log CFU/mL, and the total coliform counts ranged from 4,48 to 7,18 log CFU/mL. The number of E. coli specimens ranged from 2,96 to 5,75 log CFU/mL. Microbial insecurity was noted for commercialized curd cheese, and the need for intervention was indicated.

scholarly journals Microbial contamination in dental unit waterlines

2003 ◽  
Vol 14 (1) ◽  
pp. 55-57 ◽  
Author(s):  
Maria Cristina Monteiro de Souza-Gugelmin ◽  
Carolina Della Torre Lima ◽  
Sergio Narciso Marques de Lima ◽  
Henis Mian ◽  
Izabel Yoko Ito
Keyword(s):  
High Speed ◽  
Microbial Contamination ◽  
Water Reservoir ◽  
Plate Count ◽  
Plate Count Agar ◽  
Quality Of Water ◽  
Colony Forming Units ◽  
Dental Staff ◽  
Dental Unit Waterlines

The quality of water in a dental unit is of considerable importance because patients and dental staff are regularly exposed to water and aerosol generated from the dental unit. The aim of this study was to evaluate the occurrence of microbial contamination in dental unit waterlines. Water samples were collected aseptically from the waterlines (reservoir, triple-syringe, high-speed) of 15 dental units. After serial dilution to 1:10(6) in APHA, the samples were seeded by the pour-plate technique and cultured in plate count agar (Difco) for 48 h at 32ºC. Analysis was based on the number of colony forming units (CFU). The Wilcoxon non-parametric test indicated that the levels of water contamination were highest in the triple-syringe (13 of 15) and in the high-speed (11 of 15); both levels were higher than those of the water reservoir. There was no significant statistical difference between the level of contamination in the triple-syringe and the high-speed as determined by the Mann-Whitney test [p(H0) = 40.98%; Z = - 0.2281]. Because biofilm forms on solid surfaces constantly bathed by liquid where microorganisms are present, these results indicate that the water in the dental unit may be contaminated by biofilm that forms in these tubules.

scholarly journals The health-related microbiological quality of bottled drinking water sold in Dar es Salaam, Tanzania

2006 ◽  
Vol 5 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Gabriel R. Kassenga
Keyword(s):  
Drinking Water ◽  
Heterotrophic Bacteria ◽  
Tap Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Dar Es Salaam ◽  
Bottled Water ◽  
Faecal Coliform ◽  
Coliform Bacteria

The consumption of bottled and plastic-bagged drinking water in Tanzania has increased largely because of the deteriorating quality of tap water. It is uncertain whether these water products are safe for drinking. In this study, the microbiological quality of bottled and plastic-bagged drinking water sold in Dar es Salaam, Tanzania, was investigated. One hundred and thirty samples representing 13 brands of bottled water collected from shops, supermarkets and street vendors were analysed for total coliform and faecal coliform organisms as well as heterotrophic bacteria. These were compared with 61 samples of tap water. Heterotrophic bacteria were detected in 92% of the bottled water samples analysed. Total and faecal coliform bacteria were present in 4.6% and 3.6%, respectively, of samples analysed with a tendency for higher contamination rates in plastic-bagged drinking water. Microbiological quality of tap water was found to be worse compared with bottled water, with 49.2% and 26.2% of sampling points showing the presence of total coliform and faecal coliform organisms, respectively. The results suggest caution and vigilance to avert outbreaks of waterborne diseases from these types of drinking water.

scholarly journals Quality of bio-solids produced in a Spanish Wastewater Treatment Plant (Córdoba-Spain) and its use in agronomy along 2000-2019

2020 ◽  
Vol 1 (4) ◽  
pp. 9-15
Author(s):  
Rafael Marín Galvín
Keyword(s):  
Escherichia Coli ◽  
Organic Matter ◽  
Wastewater Treatment ◽  
Treatment Plant ◽  
The Other ◽  
Treated Wastewater ◽  
Urban Wastewater ◽  
Main Metal ◽  
Colony Forming Units

Bio-solids are the final fate of pollution present in urban wastewater, reaching the production of these ones in Spanish WWTPs 701,751 T/year (dates of 2018). Considering that 85% of Spanish bio-solids are used in agronomy, it is important to know characteristics of biosolids there produced, and in this way, we have investigated bio-solids generated in La Golondrina´s WWTP (Córdoba, Spain) along 2000-2019. This WWTP is a conventional facility operated by activated sludges (26.55x106 m3/year treated) which has produced 1.43 kg of bio-solids per m3 of treated wastewater (38.000 T/year). Our results indicated that bio-solids had a dryness over initial mass of 22.3%, and 74.9% of organic matter over dried matter (o.d.m.). At the same time, major components detected in bio-solids were N, P and Ca which levels were 5.0%, 3.5% and 3.7%, respectively. On the other hand, concentration of total metals in bio-solids ranged 13,024 mg/kg o.d.m., being the main metal Fe (11.749 mg/kg o.d.m.) followed by Zn, Cu and Mn, with levels as mg/kg o.d.m. of 463.1, 392.8 and 265.7, respectively. Evolution per year of all the investigated parameters are shown in the paper. Taking into account the use of bio-solids in agronomy, we have evaluated levels of metals limited by the Spanish normative to this respect: thus, the seven metals restricted (Cd, Cu, Ni, Pb, Zn, Hg and Cr) exhibited concentration in bio-solids very lower than parametric values established. Moreover, we have estimated the ratios of accumulation of organics and metals from wastewater to bio-solids: thus, organic matter, N and P, were accumulated in bio-solids respectively, 342, 356 and 643 times, and total metals, 2,632 times. Finally, levels of Escherichia coli slightly varied from wastewater to bio-solids: 1.5x108 colony-forming units/L in the first one, and 0.9x108/g (o.d.m.) in the second ones.

scholarly journals The rationale for introducing the indices «Generalized coliform bacteria» and «Escherichia coli» into the scheme of sanitary and microbiological control of water quality as indices of fecal contamination

2021 ◽  
Vol 99 (12) ◽  
pp. 1353-1359
Author(s):  
Angelika V. Zagainova ◽  
Galina M. Trukhina ◽  
Yury A. Rakhmanin ◽  
Tamara Z. Artemova ◽  
Marina A. Sukhina
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Contamination ◽  
Coliform Bacteria ◽  
E Coli ◽  
Intestinal Infections ◽  
Safety Of Drinking Water ◽  
Microbiological Indicators

Introduction. The increasing bacterial contamination of water bodies requires an increase in water quality control’s reliability to ensure epidemic safety against waterborne infections. Therefore, researchers in both Russia and Europe came to the conclusion that it is necessary to search for indicator microorganisms that can more accurately suggest the presence of pathogens. microorganisms in water than traditional indicators. The aim of the study was to justify the introduction of indicator indices of fecal contamination “generalized coliform bacteria” and Escherichia coli to assess the safety of drinking water Material and methods. The article provides an analysis of domestic and international regulatory documents and literary materials regulating the quality of drinking water in terms of sanitary and microbiological indicators and assessment criteria. The results of many years of experimental and field research carried out by research organizations and practical organizations of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare are presented. Results. On the territory of Russia, “general coliform bacteria,” is one of the indicator indices chosen according to the fermentation of lactose, determine the safety of drinking water. The water does not take into account pathogenic bacteria (Salmonella, Shigella) and a number of lactose-negative opportunistic bacteria, causative agents of intestinal infections. The study of microorganisms isolated from the feces of patients confirms the frequency of occurrence of lactose-negative microorganisms to varying from 20 to 100% of strains. With an annual trend towards a decrease in the percentage of non-standard drinking water samples in terms of microbiological indices, general intestinal infections (GII) of unknown etiology increase, i.e. risk of GII. If the quality of drinking water does not correspond to thermotolerant coliform bacteria (TCB), 95% of samples contain E. coli. Therefore, the determination of E. coli more reliably indicates the intake of fresh fecal contamination and provides efficiency in taking measures to eliminate an unfavorable situation than TCB. Conclusion. Reasons are given for the introduction of more reliable microbiological indicators of water safety control, such as - “generalized coliform bacteria” with the preservation of the abbreviation GCB, combining both lactose-positive and lactose-negative bacteria, determined by the sign of glucose fermentation, negative oxidase test and negative stain according to Gram and E. coli as an indicator of recent faecal contamination, which will allow the assessment of water quality for a wide range of bacteria of the order Enterobacterials, corresponding to the modern taxonomy of Enterobacteriaceae NCBI, will ensure harmonization with international requirements and the safety of drinking water for the population.

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