scholarly journals Influence of Sago in Improving a Weight Gain of Rats and the Health Profile of the Small Intestine of Rats Infected by Enteropathogenic Escherichia Coli (O127:H6)

2018 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Small Intestine ◽  
Weight Gain ◽  
Intestinal Flora ◽  
Total Body Weight ◽  
Negative Control ◽  
The Body ◽  
E Coli ◽  
Baseline Weight

Background: Sago is one potential source of traditional food contain carbohydrates and have benefits as an anti-diarrheal. Escherichia coli are a member of the normal intestinal flora. However, one of the serotypes of this bacterium, E. coli (O127: H6) can be an important cause of diarrhea in infants. Objective: To analyze the sago effect on weight gain of rats and the profile morphology of small intestine that infected by E. coli (O127: H6). Methods: An experimental research using 20 wistar rats from April to June 2015. Rats were divided into four groups; Negative control 14 days (A), giving sago for 14 days (B), giving E. coli (O127: H6) and body weight lowered 20% from baseline weight for 7 days (C), giving E. coli (O127: H6) and body weight lowered 20% from baseline weight for 7 days simultanly with giving sago for 14 days (D). Weight gain measured daily, tissue biopsy of the small intestine is processed by using paraffin embedding and stained with hematoxylin eosin. Data were analyzed using Analysis of Varian (ANOVA) and to determine the differences in respectively group continued with the Last Significant Different (LSD). Results: Total body weight increase: (A) 19.80gr ± 1.64, (B) 12.80gr ± 1.64, (C) 27.40gr ± 2.40, (D) 14.20gr ± 0.44. Percentage villi damage (%): (A) 1.80 ± 0.44, (B) 1.60 ± 0.54, (C) 28.00 ±6.70, (D) 3.80 ± 0.83. Sago decreased percentage of small intestine villi damage 24.20%. Conclusion: Sago can increase the body weight and protect the small intestine villi damage from E.coli.

scholarly journals Evaluation of antipyretic activity of hydroalcoholic extract of Corchorus depressus Linn. in Escherichia coli–induced pyretic rabbits

2018 ◽  
Vol 16 ◽  
pp. 205873921879295
Author(s):  
Saeed Ahmad ◽  
Muhammad Akram ◽  
Syed Muhammad Ali Shah ◽  
Sabira Sultana
Keyword(s):  
Escherichia Coli ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Hydroalcoholic Extract ◽  
Antipyretic Effect ◽  
E Coli ◽  
Antipyretic Activity ◽  
Experimental Treatments

This study was conducted to investigate the antipyretic effect of the hydroalcoholic extract of Corchorus depressus Linn. against Escherichia coli ( E. coli)-induced pyrexia in rabbits. Hydroalcohalic extracts of C. depressus were given orally at 25, 50, and 100 mg/kg for antipyretic affect in E. coli-induced fever in rabbits. The animals were divided into five groups of five each. Among these five groups, three received various doses of experimental treatments, whereas the fourth one served as positive control and received paracetamol. The fifth group of animals served as negative control and received no treatment. The body temperature of the rabbits was measured rectally over a period of 5 h. C. depressus exhibited better effects at dose rate of 25, 50, and 100 mg/kg. The hydroalcoholic extract of C. depressus has significant antipyretic effect. These results lend support to the popular use of C. depressus in traditional medicine as a remedy for pyrexia and suggest that the characterization of the principles for such activity deserves further investigation.

Prevalence of Escherichia coli O157:H7 and Performance by Beef Feedlot Cattle Given Lactobacillus Direct-Fed Microbials†

2003 ◽  
Vol 66 (5) ◽  
pp. 748-754 ◽  
Author(s):  
M. M. BRASHEARS ◽  
M. L. GALYEAN ◽  
G. H. LONERAGAN ◽  
J. E. MANN ◽  
K. KILLINGER-MANN
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Body Weight Gain ◽  
Live Weight ◽  
Escherichia Coli O157 ◽  
Animal Testing ◽  
Fecal Shedding ◽  
E Coli

Fecal shedding of Escherichia coli O157:H7, the prevalence of Escherichia coli O157:H7 in pens and on carcasses and hides, and cattle performance as a result of daily dietary supplementation with Lactobacillus-based direct-fed microbials (DFMs) were evaluated in a feeding trial involving 180 beef steers. Steers were evaluated for shedding of E. coli O157:H7 by an immunomagnetic separation technique on arrival at the feedlot, just before treatment with the DFMs, and every 14 days thereafter until slaughter. Composite pen fecal samples were collected every 14 days (alternating weeks with animal testing), and prevalence on hides and carcasses at slaughter was also evaluated. Feedlot performance (body weight gain and feed intake) was measured for the period during which the DFMs were fed. Gain efficiency was calculated as the ratio of weight gain to feed intake. Lactobacillus acidophilus NPC 747 decreased (P < 0.01) the shedding of E. coli O157:H7 in the feces of individual cattle during the feeding period. E. coli O157:H7 was approximately twice as likely to be detected in control animal samples as in samples from animals receiving L. acidophilus NPC 747. In addition, DFM supplementation decreased (P < 0.05) the number of E. coli O157:H7–positive hide samples at harvest and the number of pens testing positive for the pathogen. Body weight gains (on a live or carcass basis) and feed intakes during the DFM supplementation period did not differ among treatments. Gain efficiencies on a live-weight basis did not differ among treatments, but carcass-based gain/feed ratios tended (P < 0.06) to be better for animals receiving the two DFM treatments than for control animals. The results of this study suggest that the feeding of a Lactobacillus-based DFM to cattle will decrease, but not eliminate, fecal shedding of E. coli O157:H7, as well as contamination on hides, without detrimental effects on performance.

scholarly journals Prebiotic supplementation effect on Escherichia coli and Salmonella species associated with experimentally induced intestinal coccidiosis in rabbits

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10714
Author(s):  
Shawky M. Aboelhadid ◽  
Shaymaa Hashem ◽  
El-Sayed Abdel-Kafy ◽  
Lilian N. Mahrous ◽  
Eman M. Farghly ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Resistance ◽  
Bacterial Infections ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Microbiological Analysis ◽  
Bacterial Colony ◽  
Fecal Examination ◽  
E Coli

Background Coccidian infection may enhance the proliferation of gut Enterobacteriaceae. Bacterial infections in rabbits can negatively affect the body condition and cause high mortality, especially at young ages. Therefore, the effect of prebiotic supplementation on the presence of Escherichia coli and Salmonella species in rabbits experimentally infected with intestinal coccidiosis was investigated. Methods Thirty male rabbits aged 35–40 days were divided into three equal groups. These groups were; prebiotic supplemented (PS), positive control (PC), and negative control (NC) groups. The prebiotic group was supplemented with 2 g/L of Bio-Mos® until the end of the experiment. At day ten post prebiotic supplementation; the PS and PC groups were inoculated orally with 5.0 × 104 sporulated oocysts of mixed species of rabbit Eimeria. The daily fecal examination was carried out from the day 4 post-infection (PI) until the day 8 PI. At day 5 and day 8 PI, 5 rabbits from each group (PS, PC, and NC) were humanely slaughtered and parts of intestinal tissue were collected for microbiological analysis. Results There was a significant decrease (P≤ 0.05) in the oocyst count in the PS group (25.12 × 104 ± 10.36) when compared with the PC group (43.43 × 104 ± 11.52) and this decrease was continued till the end of the experiment. Eleven E. coli isolates were detected in the collected samples with an overall prevalence of 24.4%. The highest prevalence of E. coli was in the PC group (13.33%) while the lowest one was in the PS group (4.44%). Meanwhile, four Salmonella serovars were isolated with an overall prevalence of 8.89%. The NC group showed one serovar (2.22%) and PC revealed three serovars (6.67%) while the prebiotic supplemented group didn’t show any salmonella isolate. Of E. coli isolates, five isolates (O78, O125, O152, O115 and O168) showed high resistance to florfenicol and neomycin (100%). Also, of salmonella serovars, thee serovars (Salmonella entrica subsp. enterica serovar Macclesfield, Salmonella entrica Subsp. enterica serovar Canada and Salmonella entrica Subsp. enterica serovar Kisangani) showed high resistance to sulphamazole, amoxicillin and flumequin (75%) while it was sensitive to levofloxacin and ciprofloxacine (75%). The bacterial colony in this study was the same results at days 5 and 8 PI. Conclusion The use of prebiotic as prophylaxis in this experiment significantly reduced the prevalence of E. coli and salmonella associated with the intestinal coccidiosis in rabbits.

scholarly journals Populasi Bakteri Usus Halus dan Performan Ayam Kampung Silangan Kampung-Leghorn Akibat Ditambahkan Ekstrak Umbi Dahlia dalam Ransum

2021 ◽  
Vol 21 (2) ◽  
pp. 157-164
Author(s):  
Lilik Krismiyanto ◽  
Nyoman Suthama ◽  
Hanny Indrat Wahyuni
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Small Intestine ◽  
Lactic Acid ◽  
Weight Gain ◽  
Lactic Acid Bacteria ◽  
Body Weight Gain ◽  
Control Diet ◽  
Native Chicken ◽  
Tuber Extract

ABSTRACT. Penelitian bertujuan untuk mengkaji perkembangan bakteri usus halus dan performan ayam kampung silangan kampung-leghorn (KL) akibat ditambahkan ekstrak umbi dahlia dalam ransum. Ternak percobaan yang digunakan adalah persilangan ayam kampung silangan KL sebanyak 200 ekor umur 22 hari dengan rata-rata bobot badan 180,46 ± 1,21 g. Bahan perlakuan meliputi umbi dahlia, ethanol 70%, kertas saring halus serta bahan pakan penyusun ransum (jagung giling, bekatul, bungkil kedelai, tepung ikan, CaCO3 dan mineral dan vitamin mix). Rancangan percobaan yang digunakan rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan (masing-masing unit percobaan diisi 10 ekor). Perlakuan yang diterapkan meliputi: P0=Ransum kontrol/RK, P1=RK+0,39% ekstrak umbi dahlia, P2=RK+0,78 ekstrak umbi dahlia, dan P3= RK+1,17 ekstrak umbi dahlia. Parameter yang diukur meliputi populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan pertambahan bobot badan harian (PBBH). Data dianalisis dengan menggunakan uji sidik ragam dan jika berpengaruh nyata dilakukan uji beda nyata Duncan pada taraf 5%. Hasil penelitian menunjukkan bahwa penambahan ekstrak umbi dahlia berpengaruh nyata terhadap populasi bakteri asam laktat, Escherichia coli, pH digesta tiap masing-masing segmen usus halus dan PBBH. Kesimpulan adalah penambahan ekstrak umbi dahlia sampai taraf 1,17% sebagai sumber inulin di dalam ransum dapat meningkatkan populasi bakteri asam laktat, menurunkan pH dan Escherichia coli pada masing-masing segmen usus halus serta memperlambat laju digesta dan meningkatkan PBBH.  (Population of small intestine bacteria and performance of native chicken-leghorn crossbreed duo to feeding of dahlia tuber extract) ABSTRAK. This study aims to examine the development of small intestine bacteria and the performances of native chicken-leghorn crossbreed due to the addition of dahlia tuber extract in the diet. Experimental animals were 200 unsex native chicken-leghorn crossbreed, 22 days old with an average body weight of 180.46 ± 1.21 g. Treatment materials include dahlia tuber, ethanol 70%, fine filter paper and feed stuff (yellow corn, rice bran, soy bean meal, fish meal, CaCO3, and vitamin-mineral mix). The present experiment was assigned in a completely randomized with 4 treatments and 5 replications (10 birds each). The treatments were: P0=Control Diet/CD, P1=CD+0,39% dahlia tuber extract, P2=CD+0,78% dahlia tuber extract and P3=CD+1,17% dahlia tuber extract. Parameters measured were the number of Lactic Acid Bacteria (LAB), Escherichia coli, intestinal potential hydrogen (pH) (duodenal, jejunal and ileal), rate of passage and daily body weight gain (DBWG). Data were subjected to ANOVA and followed by Duncan Multiple Range Test on 5% level. The results indicated that feeding native chicken-leghorn crossbreed with the diet containing dahlia tuber extract (DTE) significantly (P0.05) increased LAB population and DBWG, decreased E. coli population and intestinal pH, and slow the rate of passage down. In conclusion, feeding of dahlia tubers as a source of inulin in the form of dahlia tuber extract to a level of 1.17% increased the population of lactic acid bacteria, reduced pH and Escherichia coli in each segment of the small intestine and retard the rate of digestion and increased body weight gain.

Selection, characterisation and evaluation of potential probiotic Lactobacillus spp. isolated from poultry droppings

2016 ◽  
Vol 7 (1) ◽  
pp. 35-44 ◽  
Author(s):  
S. Asghar ◽  
M. Arif ◽  
M. Nawaz ◽  
K. Muhammad ◽  
M.A. Ali ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Rrna Gene ◽  
Poultry Droppings

Aim of the present study was to characterise and evaluate probiotic potential of lactobacilli isolated from indigenous poultry. Lactobacilli were isolated from poultry droppings and identified by genus specific polymerase chain reaction and 16S rRNA gene sequencing. Isolates were characterised in vitro by their ability to tolerate low pH and bile salts, phytase activity, antimicrobial activity, antibiotic susceptibility profile, and autoaggregation and coaggregation with poultry gut pathogens. In vivo evaluation of selected isolates was done by their effect on the body weight gain and immune response of broiler chicks. Total of 90, one-day old chicks, were randomly divided in 9 groups and given selected lactobacilli alone and in combinations (108 cfu/bird, daily) from day 7 to day 35. Body weight gain and humoral immune response to New Castle Disease Virus (NDV) vaccine were determined weekly. Three lactobacilli isolates (SMP52, SMP64 and SMP70) were selected as potentially probiotic bacteria on the basis of in vitro characterisation and identified as Lactobacillus crispatus, Lactobacillus casei and L. crispatus, respectively. Chicks supplemented with ‘SMP52’, ‘SMP64’, ‘SMP70’ and ‘SMP64+SMP70’ and a commercial probiotic product (Protexin) showed significantly higher mean weight gain per bird (1,584±35.2, 1,629±30.6, 1,668±34.7, 1,619±29.5 and 1,576±31.7 g/bird, respectively) as compared to negative control group (1,394±26.7 g/bird), on day 35. SMP 70 also showed significantly higher geometric mean titre against NDV vaccine at day 21 as compared to negative control. It is concluded that L. crispatus SMP52, L. casei SMP64 and L. crispatus SMP70 are potential probiotic candidates which alone or in different combinations may increase body weight of broilers.

scholarly journals Effects of different types and doses of whey protein on the physiological and intestinal flora in D-galactose induced aging mice

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0248329
Author(s):  
Zuolin Ma ◽  
Fumei Zhang ◽  
Hongxin Ma ◽  
Xinghao Chen ◽  
Jiaqing Yang ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Whey Protein ◽  
Bacterial Flora ◽  
Interleukin 2 ◽  
Intestinal Flora ◽  
The Elderly ◽  
The Body ◽  
Sod Activity ◽  
High Doses

The elderly usually suffer from many diseases. Improving the quality of life of the elderly is an urgent social issue. In this present study, D-galactose treated aging mice models were used to reveal the effects of different animal sources and different doses of whey protein (WP) on the immune indexes organs and intestinal flora. A total of 9 groups were set up, including normal control (NC), negative control (NS), positive control (Vc), low-, medium- and high-doses of cow WP intervention groups (CL, CM and CH for short, correspondingly) and low-, medium- and high-doses of goat WP intervention groups (GL, GM and GH for short, correspondingly). The body weight gain, thymus/body weight ratio, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, spleen immunoglobulins G (IgG), spleen interleukin-2 (IL-2) and spleen interleukin-2 (IL-6) were measured. Then, the intestinal contents were collected, and 16s genes of intestinal bacteria were sequenced to reveal the changes in bacterial flora structure. WP intervention significantly increased the weight gain, thymus/body ratio and SOD activity, but decrease the content of MDA. WP intervention increased some immune indicators. All the WP treated aging mice showed similar values of physiological indexes to that of the Vc group, even better. The relative abundance of Lactobacillus and Stenotrophomonas was increased and decreased, respectively, by both cow and goat WP. Lactobacillus may be involved in regulating the functional repair of organisms. In contrast, Stenotrophomonas might play a negative role in the immune and antioxidant capacity of the body. Combining physiological indicators and intestinal flora structure, low-concentration WP for cow and goat might be optimal for aging models.

Thermo-stable Escherichia coli phytase improves growth performance and nutrient utilization in broilers fed mash and pelleted corn-soybean-meal-based diets

2010 ◽  
Vol 90 (4) ◽  
pp. 529-536 ◽  
Author(s):  
T.A. Woyengo ◽  
A. Emiola ◽  
A. Owusu-Asiedu ◽  
W. Guenter ◽  
P.H. Simmins ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Nutrient Utilization ◽  
Positive Control ◽  
Negative Control ◽  
Coating Technology ◽  
Phytate Phosphorus ◽  
Tibia Ash

The bio-efficacy of Escherichia coli-derived phytase produced by thermo-protective coating technology (coated phytase) was evaluated in mash or pelleted broiler diets. Three hundred and twenty-four broilers were divided into 54 groups of six birds and fed nine corn-based diets (six replicates per diet) from 1 to 22 d of age. The nine diets were a positive control (PC) (calcium, 0.9% and non-phytate phosphorus, 0.45%) fed as mash plus 2 × 4 factorial of a negative control (calcium, 0.78% and non-phytate phosphorus, 0.26%) fed as mash (NC-M) or pellet (NC-P) and with coated phytase at 0, 500, 600 or 700 FTU kg-1. The diets were pelleted at 80°C, and pressure load of 40 psi. Body weight gain (BWG) for the PC diet was higher (P < 0.001) than that for the NC-M or NC-P diet. The BWG and tibia ash were linearly increased (P < 0.001) by increased level of the phytase in NC-M or NC-P diet. The BWG for NC-M or NC-P diet reached that of the PC diet when phytase was supplemented at 600 or 700 FTU kg-1. In conclusion, the coated phytase improved nutrient utilization in broilers, and its bio-efficacy was unaffected by the pelleting process.

PENGEMBANGAN TANAMAN RAMI (Boehmeria nivea L. Gaud) DAN PEMANFAATAN LIMBAH DAUN RAMI UNTUK PENGGEMUKKAN DOMBA WONOSOBO

2018 ◽  
Vol 16 (2) ◽  
pp. 201-210
Author(s):  
Muryanto Muryanto ◽  
Pita Sudrajad ◽  
Amrih Prasetyo
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Plant Development ◽  
Body Weight Gain ◽  
Survey Methods ◽  
The Body ◽  
Boehmeria Nivea ◽  
Central Java ◽  
Potential Capacity ◽  
The Given

The aim of the study was to determine the development of ramie plants (Boehmeria nivea L. Gaud) and the effect of using ramie leaves on feed on the body weight gain of Wonosobo Sheep (Dombos). Research on the development of ramie plants using survey methods in the area of ramie plant development in Wonosobo Regency. While the research on the use of ramie leaves for fattening was carried out in Butuh Village, Kalikajar District, Wonosobo Regency in 2018. 21 male Dombos were divided into 3 feed treatments with forage proportions of 70%, 50% and 30 ramie leaves respectively. %. The results showed that currently ramie plants were being developed in Wonosobo Regency by CV. Ramindo Berkah Persada Sejahtera in Gandok Village, Kalikajar District, Wonosobo Regency, Central Java. Until now the area of the crop has reached 13 ha. Of this area will produce ramie leaves 195,000 kg / year. If one sheep needs 4 kg of ramie / tail / day leaves, then the potential capacity of sheep is 135 heads / year, if the given one is 50% then the Jurnal Litbang Provinsi Jawa Tengah, Volume 16 202 Nomor 2 – Desember 2018potential capacity is 270 heads / year and if it is reduced again to 25% of ramie leaves then the potential capacity 440 heads / year. The use of ramie leaves as a feed for Wonosobo Sheep fattening can be given as much as 30% in fresh form.

Digestibility of Nutrients as Influenced by Supplementation of Exogenous Fibrolytic Enzymes in Dry Non-pregnant Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
P. M, Lunagariya ◽  
R. S. Gupta ◽  
S. V. Shah ◽  
Y. G. Patel
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Dairy Cows ◽  
Dry Matter ◽  
Body Weight Gain ◽  
The Body ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Fibrolytic Enzymes ◽  
Nitrogen Free Extract

The study was planned to evaluate the effect of exogenous fibrolytic enzymes (EFE) supplementation for 56 days @ 240 mg/kg total mixed ration (TMR) on digestibility of dry matter and nutrients in dairy cows. Six dry non-pregnant cows were assigned in each treatment with and without EFE. The digestibility trial of seven days was conducted after 49 days of feeding. Dry matter and nutrients intake of cows was not influenced by EFE. The supplementation of EFE had improved digestibility of dry matter, organic matter, crude fiber, neutral detergent fiber, cellulose (p less than 0.01), as well as digestibility of nitrogen-free extract and acid detergent fiber, was also higher (pless than 0.05). The body weight gain of cows was higher on the supplementation of EFE in TMR. The study concluded that feeding exogenous fibrolytic enzymes (240 mg/kg) supplemented TMR improved digestibility of dry matter and nutrients, which was reflected as higher body weight gain in dry non-pregnant Gir and crossbred dairy cows.

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