scholarly journals Effect of Polyethylene Glycol Microspheres Adsorbed with Melatonin on Oxidative Stress and Viscosity of Cervical Mucus Samples Infected with Human Papillomavirus

2020 ◽  
Vol 10 (6) ◽  
pp. 6757-6772 ◽  
Keyword(s):  
Oxidative Stress ◽  
Human Papillomavirus ◽  
Polyethylene Glycol ◽  
Superoxide Anion ◽  
Therapeutic Potential ◽  
Cervical Mucus ◽  
Main Function ◽  
Melatonin Concentration ◽  
High Risk Hpv ◽  
Oncogenic Hpv

The interfaces of hormones and endogenous molecules associated with nanostructured materials is one of the ways to evaluate the therapeutic potential. Melatonin is a neurohormone that is related to oxidative metabolism. Human Papillomavirus (HPV) is the principal etiological agent of cervical cancer. Cervical mucus is a biological system whose main function is the protection of the uterine cervix. This study aims to evaluate the immunomodulatory effect of melatonin on oxidative stress and rheological behavior of cervical mucus. The cervical mucus was analyzed the melatonin concentration, viscosity, superoxide, and the superoxide dismutase. Melatonin showed lower concentration in the cervical mucus from women infected with HPV. The viscosity was lowest in mucus from women infected with oncogenic HPV. The positive samples for HPV also showed high superoxide release and reduced CuZn-SOD levels indicating oxidative stress. Melatonin adsorbed to the polyethylene glycol microsphere (PEG-MLT) was efficient in restoring the viscosity, superoxide anion, and CuZn-SOD at similar levels to mucus from women negative for HPV. These data suggest that in high-risk HPV infections, there is a decrease in melatonin levels and the viscosity of cervical mucus. PEG-MLT was efficient in reducing oxidative stress and restoring viscosity of cervical mucus.

Correlation between melatonin concentration and cytokines in cervical mucus in positive samples for the presence of human papillomavirus

2021 ◽  
Author(s):  
Aron Carlos de Melo Cotrim ◽  
Eduardo Luzia França ◽  
Jordana Santos Martins ◽  
Katleyn Polizeli Galvão Silva ◽  
Mahmi Fujimori ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Mucus ◽  
Melatonin Concentration

scholarly journals Up-regulation of peroxiredoxin 3 by high-risk human papillomavirus in cervical cancer cells

2021 ◽  
Author(s):  
Hou-Li Liu ◽  
Xiao-Juan Sun ◽  
Xiaoyan Li ◽  
Jingmin Li ◽  
Xianyong Bai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cervical Cancer Cells ◽  
Cancer Tissues ◽  
High Risk Hpv ◽  
Peroxiredoxin 3

IntroductionPeroxiredoxin 3 (PRX3) is a member of PRX family with antioxidant functions by scavenging hydrogen peroxide. Since the development of cervical cancer is causally linked to high-risk human papillomavirus (HPV) that induces oxidative stress, we conducted the present study to investigate the response of PRX3 to high-risk HPV infection.Material and methodsThis study included fifty-six patients with invasive squamous cervical cancer and sixty control patients with hysteromyoma. Enzyme-linked immunosorbent assay was performed to detect cervical oxidative stress and serum PRX3. The expression of PRX3 and oncoprotein E6 of HPV16 or HPV18 was examined in cervical cancer tissues by immunohistochemistry. Western Blot was applied to detect the expression of PRX3 and E6 in cervical cancer cell lines including CaSki, HeLa, and C33A.ResultsPatients with cervical cancer showed higher serum PRX3 than control patients with hysteromyoma. Levels of oxidative markers in cervical cancer tissues were elevated as compared to normal cervical epithelia. PRX3 expression was upregulated in cervical cancer tissues and the upregulation was positively associated with the expression of E6 of HPV16 or HPV18. The association was confirmed in HPV-containing cervical cancer cell lines including CaSki and HeLa.ConclusionsOur results indicated a positive response of PRX3 to HPV-induced oxidative stress. Serum PRX3 might be a potential indicator of active amplification of high-risk HPV in cervical cancer cells.

Antioxidants Associated with Oncogenic HPV Infection in women

2021 ◽  
Author(s):  
Hui-Yi Lin ◽  
Qiufan Fu ◽  
Yu-Hsiang Kao ◽  
Tung-sung Tseng ◽  
Krzysztof Reiss ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hpv Infection ◽  
Host Immune System ◽  
Nutrition Examination Survey ◽  
Infection Status ◽  
Dietary Antioxidants ◽  
Health And Nutrition ◽  
High Risk Hpv ◽  
Cancer Studies ◽  
Oncogenic Hpv

Abstract Background Human papillomavirus (HPV) infection is a major cause of cervical cancer. Studies showed the onset of HPV carcinogenesis may be induced by oxidative stress affecting the host immune system. The association between antioxidants and oncogenic HPV remains unclear. In this study, we aim to identify antioxidants associated with vaginal HPV infection in women. Methods The associations between the 15 antioxidants and vaginal HPV infection status (no, low-risk, and high-risk HPV [LR- and HR-HPV]) were evaluated using 11,070 women who participated in the 2003-2016 National Health and Nutrition Examination Survey (NHANES). Results We identified serum albumin and four dietary antioxidants (vitamin-A, -B2, -E, and folate) inversely associated with HR-HPV infection. Women with a low level of albumin (≤39 g/L) have a significantly higher risk of HR-HPV (OR=1.4, p=0.009 vs. >44 g/L). A nutritional antioxidant score (NAS) was developed based on these four dietary antioxidants. The women with the lowest quartile NAS had a higher chance of HR-HPV (OR=1.3, p=0.030) and LR-HPV (OR=1.4, p=0.002) compared with the women with the highest quartile NAS. Conclusions We identified five antioxidants negatively associated with vaginal HR-HPV infection in women. Our findings provide valuable insights into understanding antioxidants’ impact on HPV carcinogenesis.

scholarly journals Detection Rates of Human Papillomavirus Associated with Cervical Cancer in Women of Nizhny Novgorod

2020 ◽  
pp. 44-47
Author(s):  
NF Brusnigina ◽  
MA Makhova ◽  
OM Chernevskaya ◽  
KA Orlova ◽  
EA Kolesnikova ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
High Risk ◽  
Hpv Infection ◽  
Human Papillomaviruses ◽  
Hpv 16 ◽  
Detection Rates ◽  
High Risk Hpv ◽  
Incident Cases ◽  
Oncogenic Hpv

The purpose of the study was to assess detection rates of human papillomavirus in cervical cancer cases of Nizhny Novgorod. Materials and methods. We used the real-time polymerase chain reaction (PCR) to test samples of mucosa lining of the cervical canal and/or transformation zone taken from 630 women with cervical dysplasia of different degrees and 107 incident cases of cervical cancer that did not undergo treatment. The detection and differentiation of 14 genotypes of high-risk human papillomaviruses (HPV) was carried out using the AmpliSens® HPV HCR-genotype-FRT PRC kit. Results. The overall infection rate of women with oncogenic human papillomaviruses was 41.8%. Among the genotypes, HPV 16 (39.2%), 18 (15.5%), 33 (16.6%), and 56 (11.9%) predominated. A high prevalence of oncogenic HPV was detected in the women with cervical intraepithelial neoplasia (58.1%) and cervical cancer (90%). The spectrum of genotypes in women with neoplasia of various degrees differed. In women with CIN II and CIN III, vaccine-preventable HPV genotypes (HPV 16 and 18) playing the leading role in the development of cervical cancer were the most frequent. The same genotypes dominated in the women with invasive cervical cancer. One oncogenic HPV genotype was usually found in the infected women (69%). The high-risk HPV infection was often combined with Ureaplasma ssp (49.3%), Mycoplasma hominis (20.1%), Cytomegalovirus (21.1%), and Herpes simplex I/II (18.2%) infections. Combinations of high-risk HPV with Chlamydia trachomatis and Herpes 6 were found in 8.3% and 5% of the cases, respectively. Conclusions. Our findings proved a wide prevalence of high carcinogenic risk HPV 16 and 18 genotypes, thus indicating the expediency of using Cervarix and Gardasil vaccines registered in the Russian Federation and containing antigens to these types of virus for specific prevention of the HPV infection.

scholarly journals The Burden of Human Papillomavirus Infections Among Women in Nigeria: A Review Update

2016 ◽  
Vol 5 (2) ◽  
pp. 9
Author(s):  
Adesuyi Ayodeji Omoare ◽  
Oluwaseyi Sedowhe Ashaka ◽  
Idris Abdullahi Nasir ◽  
Olajide Olubunmi Agbede
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cultural Practices ◽  
Population Based ◽  
Multiple Infections ◽  
Therapeutic Vaccines ◽  
Cancer Of The Cervix ◽  
High Risk Hpv ◽  
Quadrivalent Vaccines ◽  
Oncogenic Hpv

Cancer of the cervix is the second largest cause of mortality among women globally with an approximate estimate of over 500,000 new cases annually. Human Papillomavirus (HPV) has been long implicated as a necessary cause of Cervical Cancer (CC) in which 80% of the disease burden occurs in developing countries where organized cytology based programmes have not been feasible. Nigeria has a population of over 47 million women who are at risk of contracting HPV. It is estimated that 14,089 women are diagnosed with Cervical Cancer and 8,240 deaths annually. This review, selected publications on HPV related Cervical Cancer across Nigeria where proper quality controlled molecular methods were used for the diagnosis of HPV. Different genotypes of high risk HPV have been detected with variations across populations. HPV types found associated with women in Nigeria included types; 16,18,31,33,35,52,56 and 68 in disproportionate distribution with cases of multiple infections. Findings from this review revealed that the prevalence of Cervical Cancer is on the increase and factors such as; poverty, ignorance, socio-cultural practices, multiple infections by more than one HPV type, persistent infection, government policy, high cost of vaccines and poor acceptance of vaccines all played important roles in the increased prevalence. There is a need for more population based studies to ascertain specific HPV genotype common to specific population and the need for the development of multivalent therapeutic vaccines over the present prophylactic bivalent or quadrivalent vaccines which does not prevent against all oncogenic HPV genotypes.

Nonalcoholic Fatty Liver Disease and Vitamin E - A Promising Relationship?

2019 ◽  
Vol 70 (1) ◽  
pp. 78-83
Author(s):  
Alexandra Totan ◽  
Daniela Gabriela Balan ◽  
Daniela Miricescu ◽  
Radu Radulescu ◽  
Iulia Ioana Stanescu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Therapeutic Potential ◽  
Plga Nanoparticles ◽  
Diet Group ◽  
Normal Diet ◽  
Oxidative Stress Biomarkers ◽  
Promising Alternative ◽  
Nonalcoholic Fatty Liver ◽  
High Caloric Diet

Oxidative stress (OS) plays an important role in NAFLD molecular mechanism. Nanoencapsulation represents a novel strategy to enhance therapeutic potential of conventional drugs. Our study analyses the encapsulated vitamin E effect on lipid metabolism and oxidative stress biomarkers in NAFLD rats. Animals were divided into 3 groups : G1 - the normal diet group; G2- the high caloric diet group ; G3 - high-caloric diet group receiving PLGA-vit E, 50 mg / kg. Serum advanced human oxidative protein (AOPP), total antioxidant capacity (TAC) and vitamin E were analysed using ELISA technique. Our results showed significant increase of G2 GPT, ALP, GGT, TG, glucose, TC and AOPP, versus G1 ( P [ 0.05) and a significant decrease of G2 serum TAC and vitamin E versus G1 results ( p = 0.01 and 0.01). Vitamin E nanoparticles (G3) caused a significant increase of TAC and significant decrease of serum AOPP, versus G2 (p [ 0.01). Results showed a significant reduction of GPT, GGT, ALP, TG and total cholesterol ( p [0.05) in G3 versus G2. PLGA nanoparticles should be considered an attractive and promising alternative to improve the bioavailability and biological activity of vitaminE.

Phytosomal Curcumin Elicits Anti-tumor Properties Through Suppression of Angiogenesis, Cell Proliferation and Induction of Oxidative Stress in Colorectal Cancer

2019 ◽  
Vol 24 (39) ◽  
pp. 4626-4638 ◽  
Author(s):  
Reyhaneh Moradi-Marjaneh ◽  
Seyed M. Hassanian ◽  
Farzad Rahmani ◽  
Seyed H. Aghaee-Bakhtiari ◽  
Amir Avan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Therapeutic Potential ◽  
Vegf Signaling ◽  
Anticancer Effects ◽  
Inhibition Of Angiogenesis ◽  
Underlying Mechanisms ◽  
Apoptotic Activity

Background: Colorectal cancer (CRC) is one of the most common causes of cancer-associated mortality in the world. Anti-tumor effect of curcumin has been shown in different cancers; however, the therapeutic potential of novel phytosomal curcumin, as well as the underlying molecular mechanism in CRC, has not yet been explored. Methods: The anti-proliferative, anti-migratory and apoptotic activity of phytosomal curcumin in CT26 cells was assessed by MTT assay, wound healing assay and Flow cytometry, respectively. Phytosomal curcumin was also tested for its in-vivo activity in a xenograft mouse model of CRC. In addition, oxidant/antioxidant activity was examined by DCFH-DA assay in vitro, measurement of malondialdehyde (MDA), Thiol and superoxidedismutase (SOD) and catalase (CAT) activity and also evaluation of expression levels of Nrf2 and GCLM by qRT-PCR in tumor tissues. In addition, the effect of phytosomal curcumin on angiogenesis was assessed by the measurement of VEGF-A and VEGFR-1 and VEGF signaling regulatory microRNAs (miRNAs) in tumor tissue. Results: Phytosomal curcumin exerts anti-proliferative, anti-migratory and apoptotic activity in-vitro. It also decreases tumor growth and augmented 5-fluorouracil (5-FU) anti-tumor effect in-vivo. In addition, our data showed that induction of oxidative stress and inhibition of angiogenesis through modulation of VEGF signaling regulatory miRNAs might be underlying mechanisms by which phytosomal curcumin exerted its antitumor effect. Conclusion: Our data confirmed this notion that phytosomal curcumin administrates anticancer effects and can be used as a complementary treatment in clinical settings.

Exploring the Chemistry and Therapeutic Potential of Triazoles: A Comprehensive Literature Review

2019 ◽  
Vol 19 (16) ◽  
pp. 1298-1368 ◽  
Author(s):  
Ankit Jain ◽  
Poonam Piplani
Keyword(s):  
Oxidative Stress ◽  
Drug Discovery ◽  
Literature Review ◽  
Structural Modification ◽  
Therapeutic Potential ◽  
Pharmacological Properties ◽  
Pharmacological Activities ◽  
Triazole Derivatives ◽  
Comprehensive Literature Review ◽  
The Common

: Triazole is a valuable platform in medicinal chemistry, possessing assorted pharmacological properties, which could play a major role in the common mechanisms associated with various disorders like cancer, infections, inflammation, convulsions, oxidative stress and neurodegeneration. Structural modification of this scaffold could be helpful in the generation of new therapeutically useful agents. Although research endeavors are moving towards the growth of synthetic analogs of triazole, there is still a lot of scope to achieve drug discovery break-through in this area. Upcoming therapeutic prospective of this moiety has captured the attention of medicinal chemists to synthesize novel triazole derivatives. The authors amalgamated the chemistry, synthetic strategies and detailed pharmacological activities of the triazole nucleus in the present review. Information regarding the marketed triazole derivatives has also been incorporated. The objective of the review is to provide insights to designing and synthesizing novel triazole derivatives with advanced and unexplored pharmacological implications.

Structurally Related Edaravone Analogues: Synthesis, Antiradical, Antioxidant, and Copper-Chelating Properties

2020 ◽  
Vol 18 (10) ◽  
pp. 779-790 ◽  
Author(s):  
Alexandre LeBlanc ◽  
Miroslava Cuperlovic-Culf ◽  
Pier Jr. Morin ◽  
Mohamed Touaibia
Keyword(s):  
Oxidative Stress ◽  
Amyotrophic Lateral Sclerosis ◽  
Therapeutic Potential ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Therapeutic Options ◽  
Partial Charge ◽  
Associated Diseases ◽  
Significant Interest ◽  
Lateral Sclerosis

Background:: The current therapeutic options available to patients diagnosed with Amyotrophic Lateral Sclerosis (ALS) are limited and edaravone is a compound that has gained significant interest for its therapeutic potential in this condition. Objectives: : The current work was thus undertaken to synthesize and characterize a series of edaravone analogues. Methods: A total of 17 analogues were synthesized and characterized for their antioxidant properties, radical scavenging potential and copper-chelating capabilities. Results: Radical scavenging and copper-chelating properties were notably observed for edaravone. Analogues bearing hydrogen in position 1 and a phenyl at position 3 and a phenyl in both positions of pyrazol-5 (4H)-one displayed substantial radical scavenging, antioxidants and copper-chelating properties. High accessibility of electronegative groups combined with higher electronegativity and partial charge of the carbonyl moiety in edaravone might explain the observed difference in the activity of edaravone relative to the closely related analogues 6 and 7 bearing hydrogen at position 1 and a phenyl at position 3 (6) and a phenyl in both positions (7). Conclusion: Overall, this study reveals a subset of edaravone analogues with interesting properties. Further investigation of these compounds is foreseen in relevant models of oxidative stress-associated diseases in order to assess their therapeutic potential in such conditions.

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