scholarly journals Labisia pumila improves wound healing process in ovariectomized rat model

2018 ◽  
Vol 13 (1) ◽  
pp. 106 ◽  
Author(s):  
Shihab Uddin Ahmad ◽  
Ahmad Nazrun Shuid ◽  
Isa Naina Mohamed
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Healing Process ◽  
Video Clip ◽  
Female Rats ◽  
Ovariectomized Rat ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Control Groups ◽  
Labisia Pumila

<p class="Abstract">The purpose of this study was to determine the wound healing effect of Labisia pumila in the ovariectomized rat model. Ninety-nine Sprague Dawley female rats equally divided into nine groups; where five were control groups, and four were treated groups. The dressing was changed daily, starting from the wound induction until complete healing. The percentage of wound contraction was measured on day 0, 2, 5, 8, 9, 10, 11, 12 and 13. Three rats were sacrificed from each group on day 2, 5 and 8 respectively for evaluating of histological assessments. Wounds dressed with extract showed considerable healing and significantly healed faster compared to all control groups (p&lt;0.05). Moreover, histological analysis revealed remarkable reduction in the scar width correlated with the enhanced collagen content and fibroblast cells, accompanied by a reduction of inflammatory cells in the granulation tissues. In conclusion, L. pumila may promote wound healing in postmenopausal rat model.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">Ovariectomy and punch biopsy: 3 min 11 sec:   <a href="https://www.youtube.com/v/IlFrHeww5Jo">Full Screen</a>   <a href="https://www.youtube.com/watch?v=IlFrHeww5Jo">Alternate</a></p>

Effects of anticoagulant drugs on wound healing process in a rat model: a comparative study

2020 ◽  
Vol 29 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Deniz Çevirme ◽  
Ömer Faruk Savluk ◽  
Eylül Kafalı Başaran ◽  
Rezzan Aksoy ◽  
Ahmet Elibol ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Healing Process ◽  
Female Rats ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Score Distribution ◽  
Significant Difference

Objective: This study aims to compare the efficacy of enoxaparin, rivoraxaban and dabigatran on wound healing using a rat model. Method: Sprague-Dawley female rats (n=56), 10–12 weeks old, weight 245±30g, were used in this study. The rats were divided into four equally-sized groups. A type 1 (secondary wound healing) and type 2 (primary wound healing) wound was opened surgically on each rat in each group. Anticoagulent drugs enoxaparin, rivoraxaban and dabigatran and physiological saline solution were administered to Groups 1, 2, 3 and 4, respectively. After wound healing was scored tissue samples were taken from euthanised rats at days five and 10 and examined histologically. Since time was used as a classification (days five and 10), a time effect was included. Results: There was no statistically significant difference in total score distribution in rats between type 1 secondary wounds for days five and 10 (p>0.05). There was no statistically significant difference in the overall score distribution in rats between type 2 primary wounds for days five and 10 (p>0.05). Conclusion: In addition to the use of low molecular weight heparin with well-known anticoagulation activity, the new generation oral medications are used efficiently in thromboembolic diseases. However, there was no evidence observed in this study that these drugs could be either beneficial or harmful to wound healing.

scholarly journals N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

2021 ◽  
Vol 22 (14) ◽  
pp. 7549
Author(s):  
Wiktor Paskal ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
Albert Stachura ◽  
Kacper Pełka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Time Points ◽  
Model Study ◽  
Sprague Dawley Rats ◽  
Animal Model Study ◽  
Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

scholarly journals Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats

2021 ◽  
Vol 33 (2) ◽  
pp. 145
Author(s):  
Hendry Rusdy ◽  
Astri Suryani Pasaribu Saruksuk ◽  
Rahmi Syaflida Dalimunte ◽  
Gostry Aldica Dohude
Keyword(s):  
Wound Healing ◽  
Tranexamic Acid ◽  
Tooth Extraction ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Jatropha Multifida ◽  
Plant Sap

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine 

scholarly journals Effect of Amniotic Fluid Stem Cells and Amniotic Fluid Cells on the Wound Healing Process in a White Rat Model

2013 ◽  
Vol 40 (5) ◽  
pp. 496 ◽  
Author(s):  
Jung Dug Yang ◽  
Dong Sik Choi ◽  
Young Kyoo Cho ◽  
Taek Kyun Kim ◽  
Jeong Woo Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Amniotic Fluid ◽  
Rat Model ◽  
Healing Process ◽  
Wound Healing Process ◽  
Amniotic Fluid Stem Cells ◽  
Amniotic Fluid Cells

scholarly journals A Novel Effective Formulation of Bioactive Compounds for Wound Healing: Preparation, In Vivo Characterization, and Comparison of Various Postbiotics Cold Creams in a Rat Model

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Nasim Golkar ◽  
Yousef Ashoori ◽  
Reza Heidari ◽  
Navid Omidifar ◽  
Seyedeh Narjes Abootalebi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bacillus Subtilis ◽  
Lactobacillus Reuteri ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Bioactive Substances ◽  
Cold Cream ◽  
Healing Property

The wound is a break in the integrity of the skin produced by injury, illness, or operation. Wound healing is an essential dynamic biological/physiological process that occurs in response to tissue damage. The huge health, economic, and social effects of wounds on patients and societies necessitate the research to find novel potential therapeutic agents in order to promote wound healing. Postbiotics, the newest member of the biotics family, are valuable functional bioactive substances produced by probiotics through their metabolic activity, which have several beneficial properties, including immunomodulatory, anti-inflammatory, antimicrobial, and angiogenesis characteristics, resulting in acceleration of wound healing. In the current study, three topical cold cream formulations containing postbiotics obtained from Lactobacillus fermentum, Lactobacillus reuteri, or Bacillus subtilis sp. natto probiotic strains were prepared. The effectiveness and wound healing activity of the developed postbiotics cold cream formulations were investigated compared to cold cream without postbiotics and no treatment via wound closure investigation, hydroxyproline content assay, and histological assessment in 25 Sprague Dawley rats divided into five groups. Interestingly, analysis of the results revealed that all three formulations containing postbiotics significantly accelerated the wound healing process. However, in general, the Bacillus subtilis natto cold cream manifested a better wound healing property. The pleasing wound healing characteristics of the topical postbiotics cold creams through the in vivo experiment suggest that formulations containing postbiotics can be considered as a promising nominee for wound healing approaches.

scholarly journals Effectiveness of Aqueous Extract of Marine Baitworm Marphysa moribidii Idris, Hutchings and Arshad, 2014 (Annelida, Polychaeta), on Acute Wound Healing Using Sprague Dawley Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Hannah Syahirah Rapi ◽  
Nor ‘Awatif Che Soh ◽  
Nurul Shahirah Mohd Azam ◽  
M. Maulidiani ◽  
Suvik Assaw ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Skin Irritation ◽  
Wound Healing Process ◽  
Histopathological Analysis ◽  
Pharmaceutical Drugs ◽  
Sprague Dawley ◽  
Normal Wound Healing ◽  
Wound Model ◽  
Ability To Regenerate

Wound healing is a well-coordinated process that restores skin integrity upon injury. However, some wound treatment poses harmful effects on the skin, which delay the normal wound healing process. Marphysa moribidii, a marine baitworm or polychaete, represents unique ability to regenerate posterior segment after injury, which may be beneficial in the wound healing treatment. The effectiveness of the polychaete as wound healing treatment was discovered through skin irritation, microbial testing, animal wound model, and chemical identifications. Three polychaete extracts (PE) emulsifying ointment (0.1%, 0.5%, and 1.0%) were topically applied to the full thickness wound model once daily for 14 days. Interestingly, PE 1.0% revealed the most rapid wound healing effects as compared to other treatments, including gamat (sea cucumber) oil (15% w/v) and acriflavine (0.1% w/v). Histopathological analysis using Masson’s trichrome staining further confirms that PE treated wound exhibited minimal scar, high collagen deposition, and the emergence of neovascularisation. The extract also displayed a minimum inhibitory concentration (MIC) of 0.4 g/ml against Escherichia coli and absence of skin irritation, infectious bacteria, and heavy metals from the extract. Moreover, chemical compounds such as alkaloid, flavonoid, amino acids, and organic acid were detected in M. moribidii extracts, which could contribute to wound healing activity. In conclusion, this study further justifies the beneficial use of polychaete in treating wound healing and could be developed as a novel bioactive agent in nutraceuticals and pharmaceutical drugs.

scholarly journals Effects of Albumin Infusion on Serum Levels of Albumin, Proinflammatory Cytokines (TNF-α, IL-1, and IL-6), CRP, and MMP-8; Tissue Expression of EGRF, ERK1, ERK2, TGF-β, Collagen, and MMP-8; and Wound Healing in Sprague Dawley Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Arie Utariani ◽  
Eddy Rahardjo ◽  
David S. Perdanakusuma
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Serum Levels ◽  
Tissue Expression ◽  
Wound Healing Process ◽  
Casein Diet ◽  
Sprague Dawley ◽  
Albumin Infusion ◽  
Sprague Dawley Rats ◽  
Protein Feed

In this study, we sought to determine the roles of albumin in wound healing, which is infused both pre- and postoperatively in malnourished patients presenting with hypoalbuminemia. For the purposes of the study, we used 25 male Sprague Dawley rats of predetermined weight and age, which were initially maintained in a standard environment and fed the same diet for 7 days prior to being segregated into one of the following five groups: A, control, normal protein feed (20% casein); B, hypoalbuminemia, 25% rat albumin infusion prior to surgery; C, hypoalbuminemia, normal protein feed (20% casein); D, hypoalbuminemia, 25% rat albumin infusion after surgery; and E, hypoalbuminemia, low-protein feed (casein 2%). The animals in all five groups were subjected to four deep incisions in their dorsal muscle fascia. On days 1, 3, 5, and 7 after surgery, ELISA was used to determine serum levels of TNF-α, IL-1, IL-6, CRP, and MMP-8, whereas immunohistochemistry was used to determine the tissue expression of EGFR, ERK1, ERK2, TGF-β, collagen, and MMP-8. Significant reductions in serum levels of TNF-α, IL-1, and CRP were detected in the groups receiving albumin infusion and the high-casein diet (P<0.05). The administration of albumin and a high-casein diet also increased the tissue expression of EGFR, ERK1, ERK2, TGF-β, and collagen and decreased that of MMP-8 relative to the hypoalbuminemia control (P<0.05). We propose that the administration of albumin promoted NF-κB signaling which, in turn, induced the transduction and transcription of factors involved in wound healing. Albumin infusion and dietary proteins play vital roles in accelerating the wound healing process, as they can contribute to correcting the hypoalbuminemic state. These findings provide insights that will contribute to our understanding of wound healing, particularly in malnourished patients.

scholarly journals Evaluation of Wound-Healing and Antioxidant Effects of Marantodes pumilum (Blume) Kuntze in an Excision Wound Model

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 228
Author(s):  
Shihab Uddin Ahmad ◽  
Nor-Ashila Binti Aladdin ◽  
Jamia Azdina Jamal ◽  
Ahmad Nazrun Shuid ◽  
Isa Naina Mohamed
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Antioxidant Activities ◽  
Healing Process ◽  
Male Rat ◽  
Normal Male ◽  
Wound Healing Process ◽  
Enzymatic Antioxidants ◽  
Collagen Iii ◽  
Antioxidant Effects

Marantodes pumilum (MP) is a great source of herbal medicine used traditionally by both men and women for various purposes. MP may have potential wound-healing effects due to its diverse biological properties. An extensive study was conducted in a normal male rat model for determining the effects of MP var. pumila (MPvp) and var. alata (MPva) on the wound healing process. Here, 126 male Sprague-Dawley rats were divided randomly into seven groups as follows: sham-operated (SH), vehicle dressing (VD), flavine dressing (FD), MPvp leaves (PL), MPvp roots (PR), MPva leaves (AL), and MPva roots (AR). The parameters studied were the percentage of wound contraction, histomorphology study by hematoxylin and eosin (H&E), Masson–Goldner trichrome (MGT), and immunohistochemistry (IHC) staining. In addition, the levels of enzymatic antioxidants and malondialdehyde were also measured in the wound tissue homogenates. Wounds treated with extracts (PL, PR, AL, and AR) showed significantly faster healing (p < 0.05) compared to untreated and control groups (SH, VD, and FD). Histological analysis among MP-treated groups revealed better re-epithelialization, higher collagen deposition, enhanced fibronectin content and fibroblast cells, and higher fiber transformation from collagen-III to collagen-I, accompanied with a significant surge in enzymatic antioxidant activities and a decline in lipid peroxidation. MP has antioxidant effects that may enhance wound healing in the rat model.

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