scholarly journals Isolation, identification and characterization of Salmonella from duck

1970 ◽  
Vol 6 (1) ◽  
pp. 7-12 ◽  
Author(s):  
T Mondal ◽  
MSR Khan ◽  
M Alam ◽  
M Purakayastha ◽  
M Das ◽  
...  
Keyword(s):  
Sensitivity Analysis ◽  
Key Words ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
Poultry Farm ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Identification And Characterization ◽  
Apparently Healthy

The present study was performed with the aim to isolate and identify Salmonella organism from diarrhoeic and apparently healthy ducks and to characterize duck Salmonella by biochemical test. Antibiotic sensitivity analysis of duck Salmonella was performed. A total of 65 cloacal samples were collected from ducks of three different regions such as Char Nilokkhiya, BAU Poultry Farm and Boyra, Mymensingh. Out of 65 samples 9 (13.07%) were found positive. The antibiotic sensitivity pattern showed that the duck isolates were highly sensitive to ciprofloxacin, kanamycin, nalidixic acid, co-trimoxazole and cephalexin but these isolates were highly resistant to chloramphenicol. Key words: Salmonella, duck, isolation, identification, characterization DOI = 10.3329/bjvm.v6i1.1331 Bangl. J. Vet. Med. (2008). 6 (1): 07-12

scholarly journals ISOLATION AND CHARACTERIZATION OF ESCHERICHIA COLI FROM BUFFALO CALVES IN SOME SELECTED AREAS OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 23-26 ◽  
Author(s):  
SK Paul ◽  
MSR Khan ◽  
MA Rashid ◽  
J Hassan ◽  
SMS Mahmud
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Rectal Swab ◽  
Antibiotic Sensitivity ◽  
Buffalo Calves ◽  
E Coli ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Highly Sensitive

The research works was conducted with a view to isolate and identify the Escherichia coli (E. coli) organism from diarrhoeic cases of buffalo reared in selected areas of Bangladesh as well the prevalence and antibiotic sensitivity pattern of the isolated E. coli in the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202 during the period from April 2008 to May 2009. A total of 50 rectal swab samples were collected from 4 different places namely Haluaghat and Boira of Mymensingh, Madupur of Tangail and Kazipur of Sirajgonj districts. The samples were aseptically carried to the laboratory of the Department of Microbiology and Hygiene and subjected to different cultural, morphological and biochemical examinations. Upon cultural, morphological and biochemical examinations 23 (45%) samples were found to be positive for E. coli. The highest prevalence was found in Haluaghat, Mymensingh (53.33%) and the lowest (40.00%) in Boira, Mymensingh and Kazipur, Sirajganj. Antibiogram study revealed that the isolated E. coli was highly sensitive to Enrofloxacin and Ciprofloxacin, moderately sensitive to Cefalexin and Amoxicillin, and resistant to Nalidixic acid and Erythromycin. DOI = 10.3329/bjvm.v8i1.7398 Bangl. J. Vet. Med. (2010). 8(1): 23-26

scholarly journals Isolation, identification, toxin profile and antibiogram of Escherichia coli isolated from broilers and layers in Mymensingh district of Bangladesh

1970 ◽  
Vol 6 (1) ◽  
pp. 1-5 ◽  
Author(s):  
MT Hossain ◽  
MP Siddique ◽  
FMA Hossain ◽  
MA Zinnah ◽  
MM Hossain ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Inoculation Test ◽  
Infant Mouse ◽  
E Coli ◽  
Heat Stable ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Poultry Farms ◽  
Increasing Trend ◽  
Apparently Healthy

The study was conducted to isolate and identify E. coli from apparently healthy broilers and layers from different poultry farms adjacent to the Bangladesh Agricultural University, Mymensingh, Bangladesh, during the period of January to May 2006 and characterize their ability to produce enterotoxin and also the antibiogram of the isolates. A total of 110 fecal samples were collected from broiler (n=55) and layer (n=55) chickens. E. coli were isolated and identified by cultural, biochemical, motility test and the heat-stable toxins were determined by Infant Mouse Assay (IMA). In case of broilers, 35 (63.6%) samples were found positive while 31 (56.4%) from layers. The overall prevalence of E. coli was 60%. Among the isolates of E. coli, 22.86% isolates from broiler and 38.71% isolates from layer were found positive for their ability to produce enterotoxin based on mice inoculation test. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to chloramphenicol, ciprofloxacin, kenamycin and cephalexin and an increasing trend of resistance was recorded in both broiler and layer isolates. It may be concluded from the results of this study that the high resistance of E. coli to antibiotics constitutes a threat to poultry industry in Bangladesh.  Key words: E. coli, toxin, antibiogram DOI = 10.3329/bjvm.v6i1.1330 Bangl. J. Vet. Med. (2008). 6 (1): 01-05

scholarly journals Isolation and Characterization of Salmonella Serovars from Buffaloes in Mymensingh, Bangladesh

2013 ◽  
Vol 1 (2) ◽  
pp. 62-64
Author(s):  
Nahid Rahman ◽  
Md Shahidur Rahman Khan ◽  
Md Mansurul Amin ◽  
Mahbubul Pratik Siddique ◽  
Jayedul Hassan ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Agglutination Test ◽  
Methyl Red ◽  
Salmonella Spp ◽  
Serological Tests ◽  
Isolation And Characterization ◽  
Highly Sensitive ◽  
Salmonella Serovars ◽  
Apparently Healthy

An investigation was carried out focusing the isolation and characterization of Salmonella serovars from buffaloes of some selected areas of Mymensingh district of Bangladesh. The objectives was to isolate and identify Salmonella serovars from diarrheic and apparently healthy buffaloes and to characterize the isolates by cultural and biochemical characteristics, serological tests and antibiotic sensitivity analysis. A total of 38 samples comprising rectal swabs and faeces were collected from 38 buffaloes originating from 3 selected areas of Mymensingh. Out of these 38 samples, 8 (20.63%) were found to be positive for Salmonella spp. All isolates fermented dextrose, maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. On the other hand, these isolates showed Indole and Voges- Proskaure test negative, Methyl-Red test positive. All these isolates subjected to rapid plate agglutination test with polyvalent “O” ( poly ?O’) and polyvalent “H” ( poly ?H’) antisera where positive agglutination were observed. All isolates were highly sensitive to ciprofloxacin, moderately sensitive to co-trimoxazole, gentamycin, tetracycline and less sensitive to erythromycin and resistant to furazolidone. DOI: http://dx.doi.org/10.3329/mh.v1i2.14092 Microbes and Health, 2012 1(2): 62-64

scholarly journals Isolation and Detection of Antibiotic Sensitivity Pattern of Escherichia coli from Ducks in Bangladesh and Nepal

2013 ◽  
Vol 1 (1) ◽  
pp. 6-8
Author(s):  
Avishek Singh ◽  
Md Shahidur Rahman Khan ◽  
Sukumar Saha ◽  
Jayedul Hassan ◽  
Urmi Roy
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Resistance Patterns ◽  
Typical Lesion ◽  
Different Origins ◽  
Biochemical Examination

A total of 120 rectal swab samples from ducks (sixty samples from Nepalgunj area of Nepal and 60 from Boyera area under Mymensingh district of Bangladesh) were collected for the isolation of Escherichia coli and their antibiogram study. After cultural and biochemical examination, a total of 40 samples from Nepalgunj and 45 samples from Boyera were found positive for E. coli. Pathogenicity study of 10 positive isolates from Nepalgunj and 12 positive isolates from Boyera were done to detect the presence of enterotoxin. All inoculated mice died showing typical lesion of extensive hemorrhage and massive edema. The isolates from two different origins showed major difference in their antibiogram study. The isolates of Nepal were highly sensitive to ciprofloxacin, co-trimoxazole, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid and; less sensitive to kanamycin and resistant to cephalexin. However, the isolates of Bangladesh were highly sensitive to ciprofloxacin, chloramphenicol and amoxicillin; moderately sensitive to nalidixic acid, cephalexin, and co-trimoxazole; less sensitive to kanamycin. This variation of antibiotic sensitivity and resistance patterns among the E. coli isolates of Nepal and Bangladesh might be due to strain variations and indiscriminate use of antibiotics in these two different countries.DOI: http://dx.doi.org/10.3329/mh.v1i1.13704 Microbes and Health Vol.1(1) June 2012 pp.6-8

scholarly journals A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase

2021 ◽  
Vol 22 (11) ◽  
pp. 6148
Author(s):  
Matteo Miceli ◽  
Silvana Casati ◽  
Pietro Allevi ◽  
Silvia Berra ◽  
Roberta Ottria ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Kinetic Constants ◽  
New Method ◽  
Monoacylglycerol Lipase ◽  
Enzymatic Cleavage ◽  
Highly Sensitive ◽  
Bioluminescence Assay ◽  
Identification And Characterization ◽  
In Cells

A novel bioluminescent Monoacylglycerol lipase (MAGL) substrate 6-O-arachidonoylluciferin, a D-luciferin derivative, was synthesized, physico-chemically characterized, and used as highly sensitive substrate for MAGL in an assay developed for this purpose. We present here a new method based on the enzymatic cleavage of arachidonic acid with luciferin release using human Monoacylglycerol lipase (hMAGL) followed by its reaction with a chimeric luciferase, PLG2, to produce bioluminescence. Enzymatic cleavage of the new substrate by MAGL was demonstrated, and kinetic constants Km and Vmax were determined. 6-O-arachidonoylluciferin has proved to be a highly sensitive substrate for MAGL. The bioluminescence assay (LOD 90 pM, LOQ 300 pM) is much more sensitive and should suffer fewer biological interferences in cells lysate applications than typical fluorometric methods. The assay was validated for the identification and characterization of MAGL modulators using the well-known MAGL inhibitor JZL184. The use of PLG2 displaying distinct bioluminescence color and kinetics may offer a highly desirable opportunity to extend the range of applications to cell-based assays.

scholarly journals Blood Culture Isolates and Antibiogram of Salmonella: The Gathering Storm

KYAMC Journal ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 188-190
Author(s):  
Ishrat Binte Reza ◽  
HAM Nazmul Ahasan ◽  
Moshtaque Ahmed ◽  
Homayra Tahseen ◽  
Tasmina Chowdhury
Keyword(s):  
Drug Resistance ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
College Hospital ◽  
Major Health ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
The World

Introduction: Typhoid fever is a common problem all over the world including Bangladesh. It is caused by salmonella typhae and paratyphae and it is an old infectious water born disease. It is encountered in Bangladesh throughout the year. For many years it is treated by Chloramphenicol and subsequently Cotrimoxazole and Amoxicillin. Despite the use of newly developed antibacterial drugs, enteric fevers caused by multidrug-resistant bacterial strains are one of major health problems in Bangladesh. Multidrug resistant Salmonella sp. has been emerged is a cause of concern. Materials and Methods: This is a retrospective study done at the Popular Medical College Hospital over a period of 6 months between January 2018 to june 2018. Results: During the study period, total 115 cases were enrolled. Regarding antibiotic sensitivity pattern, 69% strains were sensitive to Ampicillin, 100% to Cefixime and Ceftriaxone, 75% to Cotrimoxazole, 52% to Nalidixic Acid and 68% to Ciprofloxacin, 69% to Levofloxacin, 80% to Cholarphenicol. Conclusion: Ceftriaxone and Cefixime were found to be highly sensitive. So, we should be very careful for the judicious use of these valuable drug to prevent drug resistance. KYAMC Journal Vol. 10, No.-4, January 2020, Page 188-190

scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

scholarly journals Prevalent Bacteria and Their Sensitivity and Resistance Pattern to Antibiotics: A Study in Dhaka Medical College Hospital

2017 ◽  
Vol 26 (1) ◽  
pp. 52-64
Author(s):  
Mohammad Zaid Hossain ◽  
Akhtarun Naher ◽  
Pratyay Hasan ◽  
Kazi Tuba E Mozazfia ◽  
Homyra Tasnim ◽  
...  
Keyword(s):  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
Sampling Technique ◽  
Salmonella Typhi ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
College Hospital ◽  
Medical College ◽  
Sensitivity Pattern ◽  
Changes Over Time

Background and rationale: Antibiotic resistance is a global problem. Many factors are complexly related to the issue in multiple dimensions. Bangladesh is right in the middle of this great calamity, and is seeing the rise in resistant strains of several bacteria. Very sadly, the prevalent malpractice of abusing antibiotics in Bangladesh contributes to add complexity to the danger which may prove to be possibly the greatest threat humans have ever faced. There is much scarcity of medical literature in Bangladesh, on the antibiotic sensitivity pattern and prevalent microorganisms. Moreover, antibiotic sensitivity pattern changes over time and place. Again, most of the studies done in Bangladesh, concentrate on a single disease, pathogen, or specimen. This study attempts to see the prevalent microorganisms and the antibiotic sensitivity pattern in multiple types of specimens collected from Dhaka Medical College Hospital. This study also attempts to establish a way of presentation of the relevant findings which can be used in future to ensure easy comparability and contrasting of findings.Methods: The specimens were collected from the adult patients (age >12 years) admitted in the Internal Medicine ward of Dhaka Medical College Hospital, Dhaka, over a period of 6 months. The sampling technique was consecutive sampling method. Specimens which were culture positive, were only included in the study for analysis. Multiple specimens were taken.Results: S. aureus was 100% sensitive to amikacin, moxifloxacin, imipenem, meropenem, piperacillin+tazobactum combination, vancomycin, doxycycline, tetracycline, tigecycline, nitrofurantoin, azactum, linezolid and 100% resistant to cefixime. Enterobacter was 100% sensitive to penicillin, amikacin, gentamicin, netilmicin, doxycycline, tetracycline, tigecycline and 100% resistant to cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, levofloxacin, vancomycin. E. coli was 100% sensitive to imipenem, meropenem, vancomycin, tigecycline and 100% resistant to mecillinam, aztreonam. Klebsiella was 100% sensitive to flucloxacillin, colistin, vancomycin, tigecycline, linezolid and 100% resistant to nalidixic acid. Proteus was 100% sensitive to cephradine, cefoxitin, cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, amikacin, ciprofloxacin, imipenem, meropenem, netilmicin, piperacillin+tazobactum combination, tetracycline, tigecycline, azithromycin, azactum and 100% resistant to doxycycline, tetracycline, chloramphenicol and cefuroxime. Pseudomonas was 100% sensitive only to amikacin, netilmicin, and 100% resistant to cefixime, ceftazidime, ceftriaxone, cefepime, cotrimoxazole, gentamicin, ciprofloxacin, levofloxacin, imipenem, meropenem, doxycline, tetracycline, chloramphenicol. Salmonella typhi was 100% sensitive to amoxicillin, cefoxitin, cefixime, ceftriaxone, cefepime, cotrimoxazole, amikacin, netilmicin, azithromycin, chloramphenicol, azactum and 100% resistant to cephradine, doxycycline, tetracycline, nalidixic acid. MRSA was 100% sensitive to imipenem, vancomycin, teicoplanin, nitrofurantoin, linezolid and 100% resistant to cefpirome, cefoxitin, ceftazidime, cotrimoxazole, clindamycin, gentamicin, ciprofloxacin, netilmicin, tetracycline, clarithromycin. Acinetobacter was 100% sensitive to penicillin, cefuroxime, colistin, piperacillin+tazobactum combination, tigecycline, chloramphenicol and 100% resistant to cefixime, nalidixic acid. Citrobacter freundii was 100% sensitive to ceftazidime, ceftriaxone, cotrimoxazole, amikacin, gentamicin, ciprofloxacin, levofloxacin, imipenem, meropenem, netilmicin, nalidixic acid and 100% resistant to ampicillin, cefixime, nitrofurantoin.Conclusion: More and more antibiotics are becoming ineffective due to emergence of resistance. Serious actions should be taken. Awareness should be raised from the policy maker level to the physicians and patients.J Dhaka Medical College, Vol. 26, No.1, April, 2017, Page 52-64

scholarly journals Pattern of Antibiotic Sensitivity in Enteric Fever: A Tertiary Care Hospital Experience

2014 ◽  
Vol 15 (2) ◽  
pp. 122-124 ◽  
Author(s):  
Samira Rahat Afroze ◽  
Muhammad Abdur Rahim ◽  
Md. Mehedi Hasan ◽  
Farhana Afroz ◽  
Hasna Fahmima Haque ◽  
...  
Keyword(s):  
Blood Culture ◽  
Nalidixic Acid ◽  
Enteric Fever ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Drug Resistant Strains

Objectives: To describe the antibiotic sensitivity pattern ofSalmonella typhi and Salmonella paratyphi from blood culture specimens. Methods: This cross-sectional study was done in the Department of Medicine, BIRDEM from July 2009 to June 2012. Standard laboratory and microbiological procedures were followed for blood culture and antibiotic sensitivity tests. Results: Among the 97 blood culture positive samples, S. typhi was 71 (73.2%) and S. paratyphi was 26 (26.8%). Multi-drug resistant strains of S. typhi and S. paratyphi were 23 (32.4%) and 3 (11.5%) cases respectively. Azithromycin, nalidixic acid, ciprofloxacin, levofloxacin and amoxicillin resistance was also found in a good number of cases (S. typhi and S. paratyphi: 71.8% and 57.7%, 42.3% and 30.8%, 38% and 34.6%, 38% and 26.9% and 38% and 26.9% cases respectively). Nineteen (31.1%) of the 61ciprofloxacin sensitive organisms were resistant to nalidixic acid. Ceftriaxone was sensitive in 100% of S. typhi and S. paratyphi. Cefixim, ciprofloxacin, levofloxacin, imipenem were among the most common sensitive antibiotics (S. typhi and S. paratyphi: 83.1% and 73.1%, 62% and 65.4%, 53.5% and 65.4%, 76.1% and 65.4% cases respectively). Conclusion: Ceftriaxone was the most sensitive antibiotic for treating enteric fever followed by cefixim, imipenem and ciprofloxacin. However, in suspected cases of enteric fever, blood culture should be requested before prescribing antibiotic.DOI: http://dx.doi.org/10.3329/jom.v15i2.20684 J MEDICINE 2014; 15 : 122-124

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