Biological and Chemical Studies on Calycopteris floribunda leaves

1970 ◽  
Vol 4 (2) ◽  
Author(s):  
Sujit K Dey ◽  
Mohammad Shoeb ◽  
Tamanna Rob ◽  
Nilufar Nahar ◽  
M Mosihuzzaman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Salmonella Typhi ◽  
Pure Compounds ◽  
Chemical Studies ◽  
Staphyloccus Aureus ◽  
Soluble Fractions

Dichloromethane-methanol extract of leaves of Calycopteris floribunda and its aqueous 90% methanol soluble fractions showed significant antibacterial activity against Bacillus subtilis, Streptococcus pyogen, Staphyloccus aureus and Salmonella typhi. The aqueous 90% methanol and 1-butanol soluble fractions of the leaves showed significant β-glucuronidase inhibition and antioxidant activity. Two pure compounds, 3,8-di-O-methyl ellagic and 2,3,7-tri-O-methyl ellagic acids were isolated from the 1-butanol soluble fraction of the parent extract. Key words: Calycopteris floribunda, Combertaceae, antibacterial, antioxidant, β-glucuronidase, ellagic acid. Dhaka Univ. J. Pharm. Sci. Vol.4(2) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Citrus Fruit Peels in Combating Various Foodborne Pathogens

2018 ◽  
Vol 9 (2) ◽  
pp. 64-66
Author(s):  
Aziz Fatima ◽  
Maham Ghouri ◽  
Toba Sajid
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Foodborne Pathogens ◽  
Methanol Extract ◽  
Orange Peel ◽  
Salmonella Typhi ◽  
Citrus Fruits ◽  
Zone Of Inhibition ◽  
Citrus Peel ◽  
Peel Extract

Foodborne diseases are the world's main problem in a society nowadays; many phytochemicals are of great interest to scientists. Herbal medicines can be used to treat many infectious diseases. In our study, we focus on screening of antibacterial activity of the lemon (Citrus limon) and orange (Citrus sinesis) peels against various pathogens. Bioactive compounds have been found in variety of citrus fruits that have favorable impact on human health. The major component; flavonoids have been found in the peels of citrus fruits in large amount. Citrus peel has a rich amount of flavonoid and other poly methylated flavonoids (exceptional to some plants). These antibacterial compounds exhibit antibacterial activity and have wide application in both food and pharmaceuticals industries. The citrus peel extracts show effective antimicrobial activity. The antagonistic activity of selected citrus peel extracts was determined by agar well diffusion; against test organisms i.e. Escherichia coli, Salmonella typhi and Bacillus subtilis isolated from food products. Effectiveness of peel extracts are measured in terms of inhibitory zones in millimeter. The methanol extract of lemon peel displayed zone of inhibition in between 5mm to 8mm and methanol extract of orange peel displayed zone of inhibition about 10mm and 11mm. It was observed that lemon peel extract has maximum antibacterial activity against Bacillus subtilis (8mm) and minimum antibacterial activity against Salmonella typhi (5mm) whereas orange peel extract has maximum antibacterial activity against Bacillus subtilis (11mm) and minimum antibacterial activity against Salmonella typhi (10mm). Both Citrus peels extract showed considerable antibacterial activity against all tested foodborne pathogens. Our main focus was the use of herbal treatments against various infections which overcome the emergence of upcoming superbugs.

scholarly journals The radical scavenging activity of 2-2’ diphenyl -1- picrylhydrazil (DPPH) on the methanol extracts and Ethyl acetate fractions of red dragon fruit peel  (Hylocereus polyrhizus (F.A.C.Weber) Britton dan Rose)

2017 ◽  
Vol 9 (1) ◽  
pp. 79
Author(s):  
Sri Wahdaningsih ◽  
Subagus Wahyuono ◽  
Sugeng Riyanto ◽  
Retno Murwanti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Insoluble Fraction ◽  
Fruit Peel ◽  
Activity Test ◽  
Dragon Fruit

<p> </p><p>Red dragon fruit (<em>H. Polyrhizus</em>) is one of the the plants that has a great potential as natural antioxidant. This study tested the activity of radical scavenging of 2-2' diphenyl -1- pikril hidrazil (DPPH) in the methanol extract, as well as in the soluble and insoluble fractions of ethyl acetate of red dragon fruit peel. This research is carried out through various stages, such as: extraction and fractionation to obtain both insoluble fraction and soluble fractions of ethyl acetate. Antioxidant activity test is conducted by the method of thin layer chromatography and spectrophotometry.<strong> </strong>Antioxidant activity test, IC<sub>50 </sub>values of methanol extract, ethyl acetate soluble fraction, and insoluble fraction of ethyl acetate had been obtained consecutively as much as 241.19 µg /mL, 8.34  µg/mL, 46.84 µg/mL. The soluble fraction of ethyl acetate had greater antioxidant activity compared to the methanol extract and the insoluble fractions of ethyl acetate.</p>

scholarly journals Phytochemical Screening, Antimicrobial and Antioxidant Activities of Sonchus Oleraceus l (Leaves) Extracts

2020 ◽  
pp. 1-4
Author(s):  
Hatim MY Hamadnalla ◽  
◽  
Mahdi Abd Elmageed Mohammed ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Salmonella Typhi ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Sonchus Oleraceus

The objective of this study to examine phytochemical analysis of sonchus oleraceus l, (asteraceae) (moliata) extracts which were extracted by four solvents, petroleum ether, chloroform, methanol and distill water and to assess their potential antibacterial against four standard bacteria gram-positive and gramnegative (bacillus subtilis staphylococcus aureu, salmonella typhi, and pscudomona aeruginosa) and antioxidant activities. (moliata) extracts using cold maceration method to extract sonchus oleraceus l, (asteraceae) locally known as (moliata) extracts, where dpph assay and paper disc diffusion assay were employed to evaluate the antioxidant and antibacterial activities respectively. The results showed that tannins, triterpenes, a sterols, flavonoids and cardiac glycosides compounds were present in the sonchus oleraceus l, (asteraceae) lives (moliata) extracts. The methanol extract of leaves showed high antioxidant potential (77± 0.03 % ) and chloroform showed low antioxidant potential activity (4±0.09) compare with propyl gallate as standard (93± 0.010 %) against dpph. Chloroform extract was effective and give moderate zone of inhibition against staphylococcus aureus. Methanol extract was effective and showed moderate zone of inhibition against staphylococcus aureus, bacillus subtilis, salmonella typhi and pseudomonas aeruginosa.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

2020 ◽  
Vol 8 (3) ◽  
pp. 177
Author(s):  
Achmad Syarifudin Noor ◽  
Bawon Triatmoko ◽  
Nuri Nuri
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction ◽  
Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

scholarly journals ANTIBACTERIAL ACTIVITY ASSAY OF MANGROVE EXTRACTS AGAINST SALMONELLA TYPHI AND LISTERIA MONOCYTOGENES

2016 ◽  
Vol 7 (2) ◽  
Author(s):  
Apon Zaenal Mustopa ◽  
Rifqiyah Nur Umami ◽  
. Melki
Keyword(s):  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Acetone Extract ◽  
Mangrove Species ◽  
The Third ◽  
Inhibition Zones

<p><strong><br /></strong><em>The antibacterial activities of mangrove species, Avicennia marina, Sonneratia caseolaris (collected from Teluk Payo, Banyuasin, South Sumatera), Ceriops tagal, Rizhopora apiculata, and Sonneratia alba (collected from Sadai, South Bangka) were screened against Salmonella typhi and Listeria monocytogenes by agar disk diffusion assays. Extractions were conducted using organic solvents (methanol, ethyl acetate, and acetone, subsequently). Most of the extracts tested showed potential antibacterial activity against both pathogens. The methanol extracts of the bark from S. alba and the fruit from A. marina showed particularly large inhibition zones (15 mm) against S. typhi. The acetone extract of S. alba leaves showed the largest inhibition zone (14 mm) when tested against L. Monocy-togenes. Further partial purifications of selected extracts which showed strong inhibition were performed by silica gel column chromatography using various eluent compositions with different polarities. The third fraction of methanol extract from S.alba leaves eluted with chloroform:methanol (1:5) resulted in a remarkably large inhibition zone (23 mm) against S. typhi. The third and seventh fractions of acetone extract from S. alba leaves eluted with ethyl acetate:methanol (7:3) resulted in a large inhibition zones (15 mm) against L. monocytogenes. In addition, the sixth fraction of methanol extract from A. marina fruit eluted with chloroform : methanol (6:4) resulted in the largest inhibition zone (17 mm) against L. monocytogenes. These results indicated that mangrove extracts could be developed as potential biomaterials for biopharmaceutical as well as biopreservation industries.</em></p><p><em><strong>Keywords</strong>: antibacterial activity, mangrove, column chromatography</em></p>

scholarly journals Antibacterial activity in the leaves of seven bitter medicinal plants of Bangladesh

1970 ◽  
Vol 18 ◽  
pp. 128-133 ◽  
Author(s):  
MTH Molla ◽  
MS Ahsan ◽  
MT Alam ◽  
ME Haque
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ethanolic Extracts

Context: Development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. Seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study.   Objectives: Evaluation of the effectiveness of some medicinal plant extracts against four Gram-positive and five Gram-negative bacteria.  Materials and Methods: The antibacterial activity of the crude ethanolic extracts obtained from the leaves of seven medicinal plants; viz., Andrographis paniculata, Catharanthus roseus, Adhatoda vasica, Vitex vegundo, Aloe vera, Flacortia ramontchi and Nyctanthes arbortristis were tested against nine bacteria at concentrations of 300-, 400- and 500 μg/ml. Standard antibiotic disc kanamycin (30μg/ml) was used for comparison. The minimum inhibitory concentration (MIC) of ethanolic extracts of the leaves of these medicinal plants were determined by testing the extracts on four Gram-positive and five Gram-negative bacteria by serial tube dilution method.   Results: All the extracts have notable antimicrobial activities against the test organisms. The ethanolic extracts of the leaves showed the highest antimicrobial activities against Bacillus megaterium and Shigella dysenteriae for An. paniculata, Ad. vasica and Al. vera; Bacillus subtilis and Salmonella typhi for C. roseus and N. arbortristis; Staphylociccus aureus and Salmonella typhi for V. vegundo; and Bacillus subtilis and Shigella sonnei for F. ramontchi respectively. The extract of the plants had MIC values ranging from 32 to 128 mg/ml. All plant extracts showed no MIC against Shigella shiga and against Sarcina lutea only C. roseus showed MIC 128 mg/ml.   Conclusion: The results revealed that the ethanolic extracts of the plants under present investigation have notable antimicrobial activities.   Keywords: medicinal plants; antimicrobial screening; MIC; bacteria. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8788 JBS 2010; 18(0): 128-133

scholarly journals Phytochemical and Biological Investigations of Phyllanthus reticulatus

1970 ◽  
Vol 5 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Taslima Begum ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Inhibitory Activity ◽  
Full Text ◽  
Brine Shrimp ◽  
Microbial Growth ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Stem Bark ◽  
Brine Shrimp Lethality ◽  
Soluble Fractions

Scopoletin (1) was isolated from the chloroform soluble fraction of a methanol extract of the stem bark of Phyllanthus reticulates (Family: Euphorbiaceae). The petroleum ether, carbon tetrachloride and choloroform soluble fractions of this methanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. All of the partitionates showed moderate to strong inhibitory activity to microbial growth while the chloroform soluble fraction showed strongest cytotoxicity having LC50 1.99 ?g/ml. Key words: Phyllanthus reticulates, Euphorbiaceae, Scopoletin, Brine shrimp lethality bioassay, Antimicrobial Dhaka Univ. J. Pharm. Sci. Vol.5(1-2) 2006 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals ANTIOXIDANT, ANTIBACTERIAL, AND ANTICANCER ACTIVITY FROM MARINE RED ALGAE GRACILARIA EDULIS.

2019 ◽  
pp. 276-279
Author(s):  
HEMASUDHA TS ◽  
THIRUCHELVI R ◽  
BALASHANMUGAM P
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Anticancer Activity ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Red Algae ◽  
Methanol Extract ◽  
Cancer Cell Line

Objective: The marine seaweed Gracilaria edulis was collected from Rameshwaram Mandapam coast. The main objective of G. edulis was to check about the antioxidant, antibacterial, and anticancer activity. The potential use of seaweeds is to prevent colon cancer and leukemia. The crude extract of antioxidant activity was done in methanol and aqueous extract in various concentrations using DPPH assay. The antibacterial activity was analyzed in methanol extract. The antibacterial activity was tested in Gram-positive bacteria (Staphylococcus aureus) and Gram-negative (Pseudomonas) bacteria with various concentration. The anticancer activity was tested against using the breast cancer cell line (MDA-MB231) Methods: The antibacterial activity was checked by crude methanol extract using Gram-positive bacteria (S. aureus) and Gram-negative bacteria (Pseudomonas) with different concentrations. The antioxidant activity was determined using DPPH radical scavenging assay in various concentrations. The anticancer activity was done using 3-(4, 5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against breast cancer cell line (MDA-MB231). Results: The antioxidant activity of marine red algae was checked using the stable DPPH radical scavenging assay. The antibacterial activity was analyzed and shows the activity of two different bacteria strain against methanol extract. The anticancer activity was done against breast cancer cell line using MTT assay. Conclusion: Thus, the result shows that red algae G. edulis has good biological activity, and further it can be carried out in pharmaceutical, food preservatives, and cosmetic purpose.

scholarly journals Phytochemical and Biological investigations of Phoenix paludosa Roxb.

1970 ◽  
Vol 8 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Farzana Alam ◽  
Mohammad S Rahman ◽  
Md Shahanur Alam ◽  
Md Khalid Hossain ◽  
Md Aslam Hossain ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Brine Shrimp Lethality ◽  
First Report ◽  
Soluble Fractions

Lupeol (1), epilupeol (2) and β-sitosterol (3) were isolated from the n-hexane and the carbontetrachloride soluble fraction of a methanol extract of the leaves of Phoenix paludosa Roxb. The n-hexane, carbontetrachloride and chloroform soluble materials from the concentrated methanol extract were subjected toantimicrobial screening and brine shrimp lethality bioassay. All of the partitionates showed insensitivity to microbialgrowth, while the n-hexane, chloroform and methanol soluble fractions showed significant cytotoxicity having LC502.17 μg/ml, 2.77 μg/ml and 2.46 μg/ml, respectively. This is the first report of isolation of the compounds 1-3 andbioactivities of P. paludosa.Key words: Phoenix paludosa; Aracaceae; Lupeol; Epi-Lupeol; β-sitosterol; Brine shrimp lethality bioassay;Antimicrobial.DOI: 10.3329/dujps.v8i1.5329Dhaka Univ. J. Pharm. Sci. 8(1): 7-10, 2009 (June)

Sign in / Sign up

Export Citation Format

Share Document