scholarly journals Effects of frozen storage, radiation and their combined Treatments on microorganisms of freshwater mola fish Amblypharyngodon mola

2013 ◽  
Vol 37 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Md Mansurul Haque ◽  
MG Sorrowar ◽  
Harun Ur Rashid
Keyword(s):  
Irradiation Dose ◽  
Frozen Storage ◽  
Storage Period ◽  
Bacterial Count ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Radiation Doses ◽  
Combined Treatments ◽  
Bacterial Strains ◽  
Coliform Count

The study revealed that the total viable bacterial count, staphylococcal count, coliform count, faecal coliform count, Aeromonas count and total fungal counts varied from 8.8×107 to 1.27×108, 2.7×106 to 4.2×106, 9.0×104 to 1.03×105, 4.8×105 to 7.3×105, 2.7×105 to 5.0×105 and 1.6×103 to 3.5×103 cfu/gm, respectively. Seventy six bacterial strains were isolated and identified, where Staphylococcus aureus, Micrococcus varians, Aeromonas hydrophila, Klebsiella ozaenae, Bacillus subtilis, Escherichia coli, Bacillus megaterium, Klebsiella edwardsii, Pseudomonas aerugenosa and Micrococcus radiodurans were 16 (21.06%), 11 (14.47%), 9 (11.82%), 6 (7.89%), 6 (7.89%), 8 (10.53%), 6 (7.89%), 4 (5.26%), 7 (9.21%) and 3 ( 3.95% ), respectively. Total coliform, faecal coliform, Aeromonas bacteria and total fungi were found to be totally inactivated by the irradiation dose of 2.5 kGy onwards and total viable bacteria and staphylococcal bacteria were eliminated completely at the irradiation dose of 7.5 kGy. The bacterial counts were shown to be gradually decreased in all the samples from initial to final storage. The combined treatments were found more effective than that of the single treatment for reducing or eliminating the associated bacteria. The moisture contents of the studied fish were found nearly similar during initial and final storage periods and also at different radiation doses but the total protein contents decreased during final storage period and also at different radiation doses. DOI: http://dx.doi.org/10.3329/jbas.v37i1.15677 Journal of Bangladesh Academy of Sciences, Vol. 37, No. 1, 21-31, 2013

scholarly journals Isolation of Food-Borne Microorganisms from Atlantic Mackerel and Disinfection of the Raw Fish by Radiation, Low Temperature and Combination Treatments

1970 ◽  
Vol 25 (2) ◽  
pp. 105-109
Author(s):  
Manik Hossain ◽  
M Kamruzzaman Munshi ◽  
Rasheda Yasmin Shilpi ◽  
Harun Or-Rashid
Keyword(s):  
Low Temperature ◽  
Frozen Storage ◽  
Bacterial Count ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Coliform Bacteria ◽  
Isolation And Identification ◽  
Bacterial Counts ◽  
Coliform Count ◽  
Combination Treatments

An investigation was undertaken for the isolation and identification of fish-borne microorganisms from mackerel fish (Scomberomorus guttatus) collected from Savar Bazar. Radiation, low temperature (-20°C) and combination treatments were then applied for the decontamination of associated organisms. The ranges of total viable bacterial count, total coliform count, total faecal coliform count and total staphylococcal count varied from 6.5 x 104 to 1.04 x 105, 2 x 102 to 4.0 x 102, 0 to 1 x 102 and 4.4 x 104 to 3.8 x 104 cfu/g respectively, while the total fungal count was nil. Sixty-four bacterial isolates were identified including Staphylococcus (19%), Micrococcus (11%), Enterobacter (8%), Klebsiella (17%), Bacillus (19%), Escherichia (17%) and Pseudomonas (9%). To disinfect the microorganisms, the samples were irradiated at different doses (0-10.0 kGy) of gamma irradiation. Total coliform and total faecal coliform bacteria were inactivated below the detectable level at 2.5 kGy of irradiation. The number of total viable bacteria decreased by three logs when irradiated at 5.0 kGy and the number of Staphylococcus bacteria were eliminated completely after the irradiation dose of 5.0 kGy. During frozen storage, the total viable bacterial counts were gradually declined in all the samples. After six months of storage the bacterial counts were decreased about one log in all of the samples. It has been observed that combination treatments (irradiation and freezing) are more effective than the single treatment for eliminating the fish-borne bacteria. Keywords: Mackerel fish; Microbial contamination; Irradiation; Low temperatureDOI: http://dx.doi.org/10.3329/bjm.v25i2.4871 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 105-109

scholarly journals Microbiological Profile of the Traditionally Collected Industrial Raw Milk from the Milk Pocket Zones of Bangladesh

1970 ◽  
Vol 25 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Gouranga C Chanda ◽  
Gazi M Noor Uddin ◽  
Aparna Deb ◽  
Tahmina Bilkis ◽  
Sharmin Chowdhury ◽  
...  
Keyword(s):  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Plate Count ◽  
Coliform Count ◽  
Standard Plate Count ◽  
Milk Samples ◽  
Bacteriological Profile ◽  
Standard Plate ◽  
Viable Bacteria

The study was aimed to evaluate the bacteriological profile of the traditionally collected industrial raw milk from the milk pocket zones of Bangladesh. About 365 raw milk samples were collected from the milk tanker, who brought raw milk from the mother chilling centre where raw milk was chilled at 4°C following traditional method. All milk samples were subjected to perform standard plate count and total coliform count. The average standard plate count was found to be 4.37 x 106 cfu/ml and the highest occurrence of standard plate count was found to be 6.70 x 106 cfu/ml in October and the lowest (3.28 x 106 cfu/ml) in March. The highest occurrence of total viable bacteria was found to be 5.64 x 106 cfu/ml in autumn and the lowest was found to be 3.78 x 106 cfu/ml in summer. On the other hand, the average of the coliform bacterial count was found to be 3.88 x 105 cfu/ml with the highest (5.70 x 105 cfu/ml) occurrence in May and the lowest (1.90 x 105 cfu/ml) in January. Moreover, the highest occurrence of coliform count was found to be 4.84 x 105 cfu/ml in rainy season and the lowest was 2.75 x 105 cfu/ml found in winter.DOI: http://dx.doi.org/10.3329/bjm.v25i1.4849 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 17-20

scholarly journals Identification of Microbial Contamination Sources in Distilled Spirits

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Ancuța ROTAR ◽  
Cristina Anamaria SEMENIUC ◽  
Elena MUDURA ◽  
Teodora COLDEA ◽  
Carmen LAZÄ‚R POP
Keyword(s):  
Manufacturing Process ◽  
Microbial Contamination ◽  
Bacterial Count ◽  
Total Coliform ◽  
Total Bacterial Count ◽  
High Alcohol ◽  
Alcohol Content ◽  
Coliform Count ◽  
Distilled Spirits ◽  
Total Yeast

Due to their high alcohol content, distilled spirits are not susceptible to microbial contamination. Because moulds were found in samples of vodka and spirit drinks, the present study was conducted to identify the sources of microbial contamination during the manufacturing process. Total bacterial count (TBC), total yeast and mould count (TYMC) and total coliform count (TCC) were determined in water and from different processing area surfaces, TBC and TYMC in the air of processing areas and TYMC in distilled spirits samples. The source of microbial contamination of distilled spirits was microaeroflora from processing areas.

scholarly journals Bacteriological Quality Analysis of Water in Storage Tanks in A University Community, South Southern - Nigeria

2021 ◽  
Vol 6 (3) ◽  
pp. 16-23
Author(s):  
Ofonime M. Ogba ◽  
Oluwayemisi A. Olorode ◽  
Mary P. Okon ◽  
Nse O. Umoh
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Bacterial Load ◽  
Bacterial Count ◽  
Total Coliform ◽  
Nutrient Agar ◽  
Quality Analysis ◽  
Storage Tanks ◽  
Filtration Method ◽  
Coliform Count

The use of unsafe water supplies and microbial contaminated water may pose serious health challenge to users. The aim of the study was to determine the level of bacterial contamination of the various water sources and the suitability for human use and consumption in University of Calabar Community, Nigeria. A total of 30 water samples were obtained from the University storage tanks and analysed for the presence of bacteria. The level of feacal coliform count, total coliform count and heterotrophic bacterial count was analysed using membrane filtration method and standard culture method on a differential and selective media. The samples were cultured on MacConkey and Nutrient agar. The isolates obtained from the above media were subcultured into slants of nutrient agar. Isolates were subjected to Gram staining and biochemical tests. The feacal coliform, Escherichia coli was isolated from all the water samples. Total coliform counts ranged from 1cfu/ml - 92cfu/ml while total heterotrophic bacterial count ranged from 1cfu/ml to 161cfu/ml. The bacteria species isolated were Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus species, Enterobacter aerogenes, Coagulase negative Staphylococcus, Proteus species, Lactobacillus species and Listeria species. The results obtained from this study have shown high level of bacterial load which makes the water unsuitable for human consumption. Further treatment may be needed as the bacteria isolates from the water samples point to feacal contamination which may be due to inadequate treatment of water, contamination while in the storage tanks or passage through contaminated pipes supplying the community. The consumers may be at high risks of enteric bacterial infections.

scholarly journals Physico-Chemical and Bacteriological Quality of Drinking Water Sources in Calabar Municipality, Nigeria

2019 ◽  
pp. 1-22
Author(s):  
B. E. Agbo ◽  
A. V. Ogar ◽  
U. L. Akpan ◽  
C. I. Mboto
Keyword(s):  
Drinking Water ◽  
Dissolved Oxygen ◽  
Total Coliform ◽  
Water Sources ◽  
Faecal Coliform ◽  
Total Hardness ◽  
Coliform Count ◽  
Physico Chemical ◽  
The Mean

This study evaluated the physico-chemical and the bacteriological quality of five different sources of drinking water in Calabar metropolis, Nigeria, to give a fair geographical representative of the town and to contribute to our understanding of the quality of drinking water in the metropolis. The physico-chemical characteristics such as pH, temperature, turbidity, conductivity, colour, Iron, dissolved oxygen, Calcium, Magnesium, alkalinity, total hardness, Manganese, Sulphate, Chloride, Phosphate, Sodium, Zinc, Copper, total dissolved solid, Nitrate, Nitrite, Ammonia, Ammonium and Potassium were determined following the procedures prescribed by American Public Health Association Standard Method. The bacteriological analysis was carried out using the standard microbiological standard for analysis of water for total and faecal coliform count. The mean temperature of the evaluated waters ranged from 23.03°C-29.3°C, mean pH ranged from 4.37-6.76, while turbidity had a mean range of 0.16 NTU-4.13 NTU. Conductivity ranged between 39.29 µs/cm - 120.7 µs/cm, dissolved oxygen with 13.30 mg/L - 4.19 mg/L, total dissolved solids ranged from 72.4 mg/L -23.5 mg/L, while the mean for iron concentration ranged from 0.12 mg/L - 0.99 mg/L. Similarly, the mean for total hardness was 34.2 mg/L - 17.1 mg/L and 7.93 mg/L - 6.71 mg/L for total alkalinity. Others includes Manganese (0.88 mg/L - 0.02 mg/L), Magnesium (16.5 mg/L - 9.9 mg/L), Calcium (9.77 mg/L - 7.20 mg/L, Nitrate (14.6 mg/L - 3.66 mg/L), Nitrite (0.076 mg/L -0.009 mg/L), Ammonia (0.89 mg/L - 0.25 mg/L), Ammonium (0.52 mg/L - 0.013 mg/L), Zinc (1.01 mg/L - 0.34 mg/L), Chloride (5.73 mg/L - 0.364 mg/L), Fluoride (0.76 mg/L - 0.277 mg/L), Copper (0.61 mg/L - 0.18 mg/L), Sodium (2.73 mg/L - 0.180 mg/L), potassium (5.73 mg/L - 2.0 mg/L), Sulphate (14.8 mg/L - 3.69 mg/L and Phosphate with 4.8 mg/L - 3.69 mg/L. The total coliform count for bottled water ranged between 2.00 cfu/100 mL – 19.00 cfu/100 mL, the total coliform range for sachet water were 6.00 cfu/100 mL and 15.00 cfu/100 mL and no faecal coliform was detected. Public water had no growth at all, the stream and borehole bacteriological analysis ranged from 27x101 cfu/ mL - 55x101 cfu/mL and 12 cfu/100 mL - 33 cfu/100 mL for total coliform respectively. Faecal coliform ranged from 15x101 cfu/ mL - 52x101 cfu/ mL for stream and 9.00 cfu100/ mL – 16.00 cfu/100 mL for borehole. A total of seven (7) different bacteria species were isolated from the sampled drinking water sources. These included Proteus spp., Streptococcus spp., Enterobacter spp., Pseudomonas spp., E.coli, Chromobacter spp., Salmonella spp. and Enterococcus spp. This study reveals a high level of poor quality sources of water in the metropolis and makes need for urgent health intervention.

scholarly journals Bacteriological Quality Assessment of Well and Stream Water Sources from Ikwerre, Emohua and Etche Local Government Areas of Rivers State

2021 ◽  
pp. 42-55
Author(s):  
A. A. Okwelle ◽  
B. E. Asikong ◽  
J. A. Lennox
Keyword(s):  
Local Government ◽  
Stream Water ◽  
Bacterial Count ◽  
Total Coliform ◽  
Water Sources ◽  
Local Government Area ◽  
E Coli ◽  
Coliform Count ◽  
Bacteriological Quality ◽  
Rivers State

Background: Majority of the human population in semi-urban and urban areas in Nigeria are heavily reliant on well and stream water as the main source of water supply for drinking and domestic use due to the inadequate provision of potable pipe borne water. These groundwater sources can easily be fecally contaminated and thus, increase the incidence and outbreaks of preventable waterborne diseases. This study was carried out to determine the bacteriological quality of some well and stream waters sources in Ikwerre, Emohua and Etche Local Government Areas of Rivers State. Methodology: Twenty-four sampling sites with 8 from each Local Government Area were randomly selected for this study. A total of 48 samples, 2 from each of the sites were collected aseptically for 12 months. Samples were analysed using membrane filtration technique for total heterotrophic bacterial count and total coliform count. The well and stream water samples were also cultured for E. coli, Salmonella, and Vibrio sp. Results: The results show that Emohua Local Government Area had the highest total heterotrophic bacterial count of 5.2 x 103 cfu/ml. This was followed by Ikwerre LGA with total bacterial count of 4.3 x 102 cfu/ml, while Etche LGA had 3.1 x 102 cfu/ml. The total coliform count ranged from 25 cfu/100ml to 50 cfu/100ml for the three LGA’s, whereas the faecal coliform count was between 12 cfu/100ml and 20 cfu/100ml. The total coliform, E. coli, Salmonella and Vibrio counts from the three local government areas occurred above the WHO stipulated zero count in 100ml of water. Conclusion: The results from this study showed contamination of all the wells studied with faecal coliforms thus, indicating the presence of other enteric pathogens and a potential source for waterborne disease outbreaks. Well water in Ikwerre, Emohua and Etche Local Government Areas of Rivers State are not safe for drinking without additional treatment like disinfection or boiling. Periodic testing and constant monitoring of these water sources should also be done to meet up with the World Health Organization Standards in the provision of safe, clean drinking water. 

Spatial Assessment of Public Water Supplies in Densely Populated Areas of Ilorin Metropolis, Kwara State, Nigeri

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
T O Agbabiaka
Keyword(s):  
Suspended Solids ◽  
Drainage System ◽  
Enterobacter Aerogenes ◽  
Bacterial Count ◽  
Total Coliform ◽  
Total Hardness ◽  
Total Bacterial Count ◽  
Residual Chlorine ◽  
Coliform Count ◽  
Faecal Coliform Count

Water samples collected from public tap supplies at densely populated areas within Ilorin metropolis were analyzed for potability and safety. The study covered a period of twenty-four months. Physicochemical parameters such as pH, turbidity, residual chlorine, colour, suspended solids and total hardness were determined. The pH of the samples ranged from 7.0 - 7.90 while the range for residual chlorine was 0.5 - 10 mg/l. The suspended solids and total hardness ranged from 0.79 - 46.0 mg/l and 7.0 - 34mg/l respectively. Turbidity of the samples ranged from 0 – 5 NTU, while the colour range was 0 - 5 HU and temperature range was 21 - 300C. The results show that only four (20%) of the twenty samples were potable and free from pathogens while Escherichia coli, Klebsiella sp. , Salmonella sp. , Pseudomonas aeruginosa, Shigellla sp. and Enterobacter aerogenes were detected in sixteen (80%) of the samples. The viable total bacterial count ranged between 0.00 – 2.6 x 102 cfu/ml, total coliform count ranged from 0 – 75 MPN/100ml while faecal coliform count ranged from 0.00 – 2.3 x 102 cfu/ml. The presence of coliforms in the samples is indicative of faecal and non-faecal contamination along the supply network, which in some cases could be due to leaching from contaminated environment into corroded pipes along the distribution network used for conveying the treated water that passed through drainage system.

scholarly journals Effects of gamma irradiation on the shelf-life of frozen tiger shrimp, Penaeus monodon (Fabricius, 1798)

2016 ◽  
pp. 10-15
Author(s):  
MG Mustafa ◽  
MA Hossain ◽  
MJ Alam ◽  
MMR Khan ◽  
SS Nilla ◽  
...  
Keyword(s):  
Shelf Life ◽  
Penaeus Monodon ◽  
Storage Period ◽  
Bacterial Count ◽  
Total Coliform ◽  
Treatment Quality ◽  
Total Bacterial Count ◽  
Tiger Shrimp ◽  
Before And After ◽  
High Doses

The effects of three levels of irradiation (3, 6 and 9 KGy) during storage period (0, 15, 30, 45, 60, 75 and 90 days) were evaluated in frozen tiger shrimp, Penaeus monodon. Biochemical composition was analyzed before and after treatment. Quality of tiger shrimp was assessed through organoleptic, chemical (Total Volatile Nitrogen, TVN and Trimethylamine, TMA) and microbiological (Total Bacterial Count (TBC); Total Mold Counts (TMC); Total Yeast Count, (TYC); Total Coliform Count, (TCC) and Salmonella count) evaluation at an interval of 15 days during the storage period. High doses of radiation affected on the texture of fish. While, the maximum shelf life was observed for 9 KGy irradiated sample. All the samples were acceptable during 90 days of storage period because the upper limit of all spoilage indicators did not exceed the recommended values.International Journal of Natural Sciences (2014), 4(1) 10-15

scholarly journals Effect of freezing period on the microbial and nutrient quality of pool barb, Puntius sophore from Dhaka city retail market, Bangladesh

2015 ◽  
Vol 41 (1) ◽  
pp. 1-11
Author(s):  
Md Ghulam Mustafa ◽  
Tamanna Yasmin ◽  
Saima Sharif Nilla ◽  
Md Mahmudur Rahman Khan ◽  
Md Anisur Rahman Khan
Keyword(s):  
Storage Period ◽  
Bacterial Count ◽  
Total Coliform ◽  
Dhaka City ◽  
Nutrient Quality ◽  
Local Markets ◽  
E Coli ◽  
Significant Difference ◽  
Puntius Sophore

The microbiological and nutrient quality of pool barb (Jatpunti), Puntius sophore collected from three local markets - Ananda bazaar, New Market and Polashi bazaar of Dhaka, Bangladesh were assessed. The investigation confirmed more or less higher microbial load in Ananda bazaar samples than the others. The microbial quality varied with market sources as total bacterial counts (TBC), total coliform counts (TCC) and faecal coliform counts (FCC) occurred in all storage period and that crossed the acceptable limit. All studied parameters except E. coli were significantly different at 7 days and 30 days of storage period and a significant difference (p < 0.05) was found in case of E. coli for all storage period where every 30 days storage showed one log reduction of bacterial count from every initial stage. The Salmonella shigella (SS) and Vibrio spp. were identified in all samples of studied local markets. An inverse relation was found between moisture and protein, moisture and fat, whereas the moisture content was increased after 30 days of freezing storage. This study concludes that the quality of P. sophore (Jatpunti) available in the studied three local markets of Dhaka city were neither satisfactory level nor to the standard for normal consumption.Bangladesh J. Zool. 41(1): 1-11, 2013

scholarly journals Impact of temperature and radiation on elimination of associated bacteria from preserved dried prawn, Macrobrachium lamerrei

2016 ◽  
Vol 3 (1) ◽  
pp. 1-7
Author(s):  
Md Enamul Haque ◽  
Tahmina Afroz ◽  
H Rashid
Keyword(s):  
Combined Treatment ◽  
Total Coliform ◽  
Treatment Temperature ◽  
Single Treatment ◽  
Combined Treatments ◽  
Bacterial Strains ◽  
Associated Bacteria ◽  
Combination Treatments ◽  
Different Temperatures ◽  
The Impact

The impact of different temperatures (0, 40, 60, 80 and 100° C for an hour) and radiation (0, 2.5, 5.0, 7.5 and 10.0 kGy) and combined treatment (temperature and radiation) for decontamination of bacteria from dried prawn, Macrobrachium lamarrei was also investigated under laboratory condition.The total viable bacterial (TVB), total staphylococcal (TS), total coliform (TC), total faecal coliform (TFC), total Aeromonas ( TA) and total fungal (TF) count were ranged from 2.7×108 to 5.3×108 cfu/g, 2.2×106 to 6.0×106 cfu/g, 2.9×104 to 9.5×104 cfu/g, 2.8×103 to 8.1×103 cfu/g, 5.7×103 to 8.5×103 cfu/g and 1.6×104 to 3.8×104 cfu/g respectively. Seventy eight bacterial strains were isolated and identified out of which 18 (23.07 %) were Staphylococcus aureus, 11 (14.10% ) were Micrococcus varians, 8 (10.25%) were Aeromonas hydrophila, 5 (6.41%) were Klebsiella ozaenae, 7 (8.97%) were Bacillus subtilis, 7 (8.97%) were Escherichia coli, 5 (6.41%) were Bacillus megaterium, 7 (8.97%) were Klebsiella edwardsii, 6 (7.69%) were Pseudomonas aeruginosa and 4 (5.12%) were Micrococcus radiodurans. Inactivated was more favorable at 100° C for TC, TFC, TA and TF and at 5.0 kGy for TA and TF and combined treatments (40° C+2.5 kGy, 60° C+2.5 kGy and 80° C+2.5 kGy) for TFC, TA and TF respectively. TS, TC and TVB were completely eliminated in treatment 40° C+5.0 kGy, 60° C+2.5 kGy, 80° C+2.5 kGy, 40° C+7.5 kGy, 60° C+7.5 kGy and 80° C+7.5 kGy respectively. Results demonstrated that the combination treatments were more effective than single treatment for eliminating the associated microorganisms/bacteria from dried prawn.Jahangirnagar University J. Biol. Sci. 3(1): 1-7, 2014 (June)

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