scholarly journals Cytotoxic Studies on Two Meliaceae plants: Chukrasia Tabularis and Aglaia Roxburghiana

2009 ◽  
Vol 1 (2) ◽  
pp. 399-402 ◽  
Author(s):  
M. K. Islam ◽  
I. Z. Eti ◽  
J. A. Chowdhury
Keyword(s):  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Cytotoxic Agent ◽  
Biological Investigation ◽  
Brine Shrimp Lethality ◽  
Vincristine Sulphate ◽  
Cytotoxic Studies ◽  
Chukrasia Tabularis

The paper details a biological investigation on chukrasia tabularis and aglaia roxburghiana, species of meliaceae. The methanol crude extract of chukrasia tabularis and aglaia roxburghiana have been screened for antitumor potentials using brine shrimp lethality bioassay with ten different concentrations. An established cytotoxic agent vincristine sulphate has been used to compare the results. From the graphs the concentration of methanolic crude extract yields the value of LC50 (50% mortality) as 1.58mg/ml and 2.21mg/ml for chukrasia tabularis and aglaia roxburghiana, respectively. In the same way the concentration at which 90% mortality (LC90) occurs for the methanol extract are obtained from the graphs and the values have been found to be 141.41mg/ml and 70.70mg/ml for chukrasia tabularis and aglaia roxburghiana, respectively. Keywords: Chukrasia tabularis; Aglaia roxburghiana; Meliacea; Cytotoxicity. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i2.1645  

scholarly journals Biological Activity Study on a Malvaceae Plant: Bombax ceiba

2011 ◽  
Vol 3 (2) ◽  
pp. 445-450 ◽  
Author(s):  
M. K. Islam ◽  
J. A. Chowdhury ◽  
I. Z. Eti
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Biological Investigation ◽  
Brine Shrimp Lethality ◽  
Vincristine Sulphate

The work described in this paper details the biological investigation on Bombax ceiba, species of Malvaceae. The methanol crude extract of Bombax ceiba was fractionated with kupchan method and n-hexane, carbon tetrachloride, chloroform fraction were made for  screening the antimicrobial and antitumor potentials using disc diffusion method and brine shrimp lethality bioassay respectively. An established antibiotic (kanamycin, 30µg/disc) and cytotoxic agent (vincristine sulphate) were used to compare the results. From the graphs the concentration of methanolic crude extract give LC50 (50% mortality) value of 3.90mg/ml. LC90 was also determined from the graph to establish the therapeutic index and the value was found 150.0mg/ml. The four fractions were assayed for antimicrobial screening and the carbon tetrachloride fraction showed most prominent zone of inhibition against a number of bacterial and fungal strains.Keywords: Bombax ceiba; Brine shrimp lethality bioassay; Disc diffusion method.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i2.5162                J. Sci. Res. 3 (2), 445-450 (2011)

scholarly journals Investigation of Antioxidant, In-vitro Cytotoxic, and In-vivo Antitumor Effects of Leaf Extracts of Annona Reticulata

2016 ◽  
Vol 29 (2) ◽  
pp. 70-74
Author(s):  
Shashwata Biswas ◽  
Mohammad Shahriar ◽  
Jahan Ara Khanam ◽  
Chowdhury Rafiqul Ahsan
Keyword(s):  
Petroleum Ether ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Control Group ◽  
Percentage Increase ◽  
Initial Weight ◽  
Antitumor Effects ◽  
Brine Shrimp Lethality ◽  
Annona Reticulata

This study was carried out to evaluate the antioxidant, brine shrimp lethality, bioassay and antitumor effects of nhexane, petroleum ether, methanol and chloroform extracts of Annona reticulata leaves. All extracts demonstrated good cupric reducing activity with increasing concentrations keeping methanol extract at the top and n-hexane extract at the second position. The methanol extract was found to have the highest antioxidant capacity 28±26.88 followed by n-hexane 16.95±11.39 and chloroform 12.85±10.32 in the form of mg of ascorbic acid equivalent per gram of crude extract. The methanol extract also showed the highest values of 2.41±0.963 mg of gallic acid equivalent phenolic content and 6.54±4.08 mg of quercetin equivalent flavonoid content per g of crude extract. In the brine shrimp lethality bioassay, LC50 values of methanol, chloroform, petroleum ether, and n-hexane extracts were found to be 1.084?g/ml, 1.096?g/ml, 1.995?g/ml and 3.126 ?g/ml, respectively. These findings suggest that A. reticulata leaves can be a good source of antioxidants and possess significant cytotoxic effect. In a narrow spectrum investigation on antitumor activity of the extracts, we found that the methanol extract inhibited the tumor growing process (control group, chloroform group and petroleum ether group showed significant rise in body weight; i.e., 8% - 14% of initial weight compared to that of methanol group showing a rise of 0.45% - 0.94% of initial weight) and improved ILS (Percentage Increase in Life Span) with increasing doses (Doses of 50mg/kg, 100mg/kg and 200mg/kg showed ILS values of 13.70%, 19.20% and 21.92%, respectively) to some extent when compared with control group and groups receiving other extracts.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 70-74

scholarly journals Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

2015 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Rokeya Siddiqui ◽  
Md Masud Alam ◽  
Mohammad Ruhul Amin ◽  
AFM Shahid Ud Daula ◽  
MM Hossain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate ◽  
Soluble Fractions

Bangladesh possesses a rich flora of medicinal plant. Out of the estimated 5000 species of phanerogams and pteridophytes growing in this country, more than a thousand are regarded as having medicinal properties. The aim of this study was to evaluate the in vitro antimicrobial activity and brine shrimp lethality of the whole plant extracts of Spilanthes paniculata, a plant belonging to the family Asteraceae. The crude extract, n-Hexane, chloroform and ethyl acetate soluble fraction of crude extract showed significant to moderate antimicrobial activity against three Gram positive and three Gram negative microorganisms. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, choloform and ethyl acetate soluble fractions were found to be 14.89 mm-19.40 mm, 14 mm-19.40 mm, 10.66 mm-13.50 mm and 9.50 mm-13.26 mm, consecutively at a concentration of 30 µg/disc. N-Hexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Spilanthes paniculata were screened for antitumor properties using brine shrimp lethality bioassay. From the results of the brine shrimp lethality bioassay, it can be well predicted that n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract possess mild cytotoxicity on shrimp naupalii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 48.978 mg/ml, 92.61 mg/ml and 216.770 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22743 Stamford Journal of Microbiology, Vol.3(1) 2013: 1-5

scholarly journals Phytochemical analysis and cytotoxicity evaluation of flowering buds of Bauhinia variegata L.

2021 ◽  
Vol 67 (3) ◽  
pp. 27-36
Author(s):  
Anham Shahid Abbasi ◽  
Qazi Najam-Us-Saqib ◽  
Atta-Ur-Rehman ◽  
Nisar-Ur-Rahman
Keyword(s):  
Aqueous Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Raw Materials ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Bauhinia Variegata ◽  
Cytotoxic Studies

Summary Introduction: Bauhinia variegata is used in traditional medicine in Pakistan. Objective: The present study was designated to analyze phytochemical screening and cytotoxicity assay of crude aqueous and methanol extracts of flowering buds of Bauhinia variegata. Methods: Qualitative and quantitative phytochemical screening of both extracts were performed by using standard protocols while brine shrimp lethality assay was conducted for cytotoxicity analysis of both extracts. Results: The highest levels of phenolics, flavonoids and terpenoids were observed in methanol extract with respective standard drugs. The aminoacid content was highest followed by phenol and flavonoids in crude aqueous extract of the raw materials. In cytotoxic studies (brine shrimp lethality assay) the use of methanol extract was reported as potent with LD50 of 241.778 µg/ml as compared to aqueous extract with LD50 of 489.7061 µg/ml. Conclusion: This study reveals that the presence of bioactive compounds in both extracts are responsible for the treatment of various ailments and being utilized by the traditional folks as a food as well. And showed that methanol extract possess cytotoxic activity and thus needs further scientific intervention for its value addition.

scholarly journals Chemical and Biological Investigation of Leaves of Polygonum plebejum

1970 ◽  
Vol 2 (2) ◽  
pp. 66-71 ◽  
Author(s):  
Md Kamrul Hasan ◽  
Abul Kalam Lutful Kabir ◽  
Sabyasachy Mistry
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
13C Nmr ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Pharmaceutical Sciences ◽  
Disc Diffusion ◽  
Biological Investigation

The cytotoxic and antimicrobial activity of methanol crude extract and column fractions of the extract of theleaves of P. plebejum were examined by brine shrimp lethality bioassay and disc diffusion methodrespectively. The extracts showed significant cytotoxic as well as antimicrobial activities. Silica gel columnchromatography of methanol extract of P. plebejum afforded a steroid. The structure was elucidated on thebasis of spectral analysis, including 1H NMR and 13C NMR and also by comparing with data in the literature. Key words: Polygonum plebejum; Polygonaceae; Stigmasterol; Cytotoxicity.DOI: 10.3329/sjps.v2i2.5826Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 66-71

scholarly journals TOXICITY TEST OF EXTRACTS OF THE FOUR SEA CUCUMBER (FAMILY HOLOTHURIIDAE) FROM EAST PENJALIRAN ISLAND, SERIBU ISLANDS, JAKARTA BASED ON THE BRINE SHRIMP LETHALITY TEST (BSLT)

2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Arum Albuntana ◽  
Yasman Yasman ◽  
Wisnu Wardhana
Keyword(s):  
Crude Extract ◽  
Brine Shrimp ◽  
National Park ◽  
Sea Cucumber ◽  
Bioactive Compound ◽  
Active Species ◽  
Brine Shrimp Lethality ◽  
Water Fraction ◽  
Lc50 Value ◽  
Toxicity Level

<p>Sea cucumber is one of the marine bioactive compounds resources . The properties of the bioactive compound are biologically shown by their functions as anticancer, antifungi, hemolytic, and immunomodulatoryt. The purpose of this research is to predict (determine) the toxicity level of the extracts of Actinopyga miliaris, Holothuria leucospilota, Bohadschia argus, and Bohadschia marmorata collected from Penjaliran Timur Island, Seribu Island National Park Jakarta. The method used in this research is Brine Shrimp Lethality Test (BSLT). The result of this research showed that all of the extract from those species are active in BSLT, indicated by LC50 values of less than 1000 μg/ml. Bohadcshia argus is highest active species indicated by LC50 value 69,254 μg/ml. Water fraction of H. leucospilota’s crude extract is the most active fraction indicated by LC50 50,968 μg/ml.</p><p>Keywords: Brine Shrimp Lethality Test, Crude Extract, Fractionation, Family Holothuriidae, Sea Cucumber</p>

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of Urtica Crenulata

2009 ◽  
Vol 2 (1) ◽  
pp. 169-177
Author(s):  
M. A. Rahman ◽  
M. S. Rana ◽  
M. M. Zaman ◽  
S. A. Uddin ◽  
R. Akter
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects.  Keywords: Urtica crenulata;  Antioxidant; Antibacterial; Cytotoxic; BHT. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2872             J. Sci. Res. 2 (1), 169-177 (2010) 

scholarly journals In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Jahangir Khan Achakzai ◽  
Muhammad Anwar Panezai ◽  
Muhammad Ayub Kakar ◽  
Abdul Manan Kakar ◽  
Shahabuddin Kakar ◽  
...  
Keyword(s):  
Cell Line ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Aqueous Fraction ◽  
Brine Shrimp Lethality ◽  
Butanol Fraction ◽  
Whole Plant ◽  
Brine Shrimp Lethality Assay ◽  
Anti Inflammatory ◽  
Mcf 7

In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.

scholarly journals 6. Cytotoxic Potential of n-Hexane Extract of Calotropis gigantea L. Leaves

2016 ◽  
Vol 1 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Kartini Hasballah ◽  
Murniana Murniana ◽  
Al Azhar ◽  
Nurul Rahmi
Keyword(s):  
Liquid Chromatography ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Cytotoxic Potential ◽  
Calotropis Gigantea ◽  
Brine Shrimp Lethality ◽  
Vacuum Liquid Chromatography

The present study was done to determine the cytotoxic potential of n-hexane extract of Calotropis gigantea L. leaves and its fractions. Here, dried leaves of C. gigantea L. were macerated using n-hexane to obtain crude extract of 21.16 g (1.03%). The components of n-hexane extract of C. gigantea L. leaves were separated with vacuum-liquid chromatography resulted 4 fractions which are A (0.5 g), B (0.9g), C (5.29 g), and D (6.25 g). Phytochemical screening indicated that the extract contained various secondary metabolic compounds such as steroids, terpenoids, saponins, flavonoids, coumarins, and phenolics. Cytotoxic potenital of the crude extract of C. gigantea L. and its fractions was examined using brine shrimp lethality test (BSLT). The LC50 values of C. gigantea’s n-hexane extract and its fractions that determined by Finney method were 272.27 (n-hexane extract), 31.62 (fraction A), 43.65 (fraction B), 33.89 (fraction C) and 20.98 (fraction D) ?g/mL, respectively. The most active was D fraction contained terpenoids, flavonoids, and phenolics which were assumed contribute to its cytotoxic potential. These results suggested that n-fraction of C. gigantea might possess antitumor or pesticidal activities..

Sign in / Sign up

Export Citation Format

Share Document