scholarly journals Biological Activity Study on a Malvaceae Plant: Bombax ceiba

2011 ◽  
Vol 3 (2) ◽  
pp. 445-450 ◽  
Author(s):  
M. K. Islam ◽  
J. A. Chowdhury ◽  
I. Z. Eti
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Biological Investigation ◽  
Brine Shrimp Lethality ◽  
Vincristine Sulphate

The work described in this paper details the biological investigation on Bombax ceiba, species of Malvaceae. The methanol crude extract of Bombax ceiba was fractionated with kupchan method and n-hexane, carbon tetrachloride, chloroform fraction were made for  screening the antimicrobial and antitumor potentials using disc diffusion method and brine shrimp lethality bioassay respectively. An established antibiotic (kanamycin, 30µg/disc) and cytotoxic agent (vincristine sulphate) were used to compare the results. From the graphs the concentration of methanolic crude extract give LC50 (50% mortality) value of 3.90mg/ml. LC90 was also determined from the graph to establish the therapeutic index and the value was found 150.0mg/ml. The four fractions were assayed for antimicrobial screening and the carbon tetrachloride fraction showed most prominent zone of inhibition against a number of bacterial and fungal strains.Keywords: Bombax ceiba; Brine shrimp lethality bioassay; Disc diffusion method.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i2.5162                J. Sci. Res. 3 (2), 445-450 (2011)

scholarly journals Cytotoxic Studies on Two Meliaceae plants: Chukrasia Tabularis and Aglaia Roxburghiana

2009 ◽  
Vol 1 (2) ◽  
pp. 399-402 ◽  
Author(s):  
M. K. Islam ◽  
I. Z. Eti ◽  
J. A. Chowdhury
Keyword(s):  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Cytotoxic Agent ◽  
Biological Investigation ◽  
Brine Shrimp Lethality ◽  
Vincristine Sulphate ◽  
Cytotoxic Studies ◽  
Chukrasia Tabularis

The paper details a biological investigation on chukrasia tabularis and aglaia roxburghiana, species of meliaceae. The methanol crude extract of chukrasia tabularis and aglaia roxburghiana have been screened for antitumor potentials using brine shrimp lethality bioassay with ten different concentrations. An established cytotoxic agent vincristine sulphate has been used to compare the results. From the graphs the concentration of methanolic crude extract yields the value of LC50 (50% mortality) as 1.58mg/ml and 2.21mg/ml for chukrasia tabularis and aglaia roxburghiana, respectively. In the same way the concentration at which 90% mortality (LC90) occurs for the methanol extract are obtained from the graphs and the values have been found to be 141.41mg/ml and 70.70mg/ml for chukrasia tabularis and aglaia roxburghiana, respectively. Keywords: Chukrasia tabularis; Aglaia roxburghiana; Meliacea; Cytotoxicity. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i2.1645  

scholarly journals New Diarylheptanoid from Garuga pinnata Roxb

2013 ◽  
Vol 61 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mst Taslima Khatun ◽  
M. Mahboob Ali Siddiqi ◽  
Al-Mansur MA ◽  
MH Sohrab ◽  
AFM Mustafizur Rahman ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Microbial Growth ◽  
Stem Bark ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wide Range ◽  
Bacteria And Fungi

9´-Desmethylgaruganin I has been isolated from the dichloromethane extract of the stem bark of Garuga pinnata Roxb. The crude extract was screened for antimicrobial activity against a wide range of gram-positive and gram-negative bacteria and fungi by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The dichloromethane extract showed moderate inhibitory activity to microbial growth and weak cytotoxicity having LC90 25.703?g-mL–1 DOI: http://dx.doi.org/10.3329/dujs.v61i2.17058 Dhaka Univ. J. Sci. 61(2): 131-134, 2013 (July)

scholarly journals Screening of antimicrobial potential and brine shrimp lethality bioassay of the whole plant extract of Spilanthes paniculata Wall. ex DC

2015 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Rokeya Siddiqui ◽  
Md Masud Alam ◽  
Mohammad Ruhul Amin ◽  
AFM Shahid Ud Daula ◽  
MM Hossain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate ◽  
Soluble Fractions

Bangladesh possesses a rich flora of medicinal plant. Out of the estimated 5000 species of phanerogams and pteridophytes growing in this country, more than a thousand are regarded as having medicinal properties. The aim of this study was to evaluate the in vitro antimicrobial activity and brine shrimp lethality of the whole plant extracts of Spilanthes paniculata, a plant belonging to the family Asteraceae. The crude extract, n-Hexane, chloroform and ethyl acetate soluble fraction of crude extract showed significant to moderate antimicrobial activity against three Gram positive and three Gram negative microorganisms. The zones of inhibition produced by the crude extract (methanolic extract), n-hexane, choloform and ethyl acetate soluble fractions were found to be 14.89 mm-19.40 mm, 14 mm-19.40 mm, 10.66 mm-13.50 mm and 9.50 mm-13.26 mm, consecutively at a concentration of 30 µg/disc. N-Hexane, chloroform and ethyl acetate soluble fractions of methanolic extract of Spilanthes paniculata were screened for antitumor properties using brine shrimp lethality bioassay. From the results of the brine shrimp lethality bioassay, it can be well predicted that n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract possess mild cytotoxicity on shrimp naupalii. The median lethal concentration (LC50, the concentration at which 50% mortality of brine shrimp nauplii occurred) of n-hexane, chloroform and ethyl acetate were 48.978 mg/ml, 92.61 mg/ml and 216.770 mg/ml, respectively, comparison with positive control vincristine sulphate with 0.839 mg/ml. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22743 Stamford Journal of Microbiology, Vol.3(1) 2013: 1-5

scholarly journals Evaluation of antioxidant activity and cytotoxic potential of Cryp-tocoryne ciliata

2013 ◽  
Vol 2 (2) ◽  
pp. 38-41
Author(s):  
Ummey Jannatun Nahar ◽  
Shah Marzia Mahjabin Lina
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Cytotoxic Activities ◽  
Brine Shrimp Lethality ◽  
Whole Plant ◽  
Vincristine Sulphate

Cryptocoryne ciliata belonging to the Araceae family has been investigated for isolation of its secondary metabolites and evaluation of biological activities of the crude extractives with special emphasis to the antioxidant activity and brine shrimp lethality bioassay. The whole plant was extracted with methanol and concentrated extract was partitioned using petroleum ether, carbon tetrachloride and ethyl acetate. Aqueous soluble fraction of the methanolic extract showed the highest antioxidant activity. The carbon tetrachloride soluble fraction of the methanolic extract and the ethyl acetate soluble fraction of the methanolic extract showed moderate antioxidant activity as compared to free antioxidant activity of tert-butyl-1-hydroxytolunene. In the brine shrimp lethality bioassay, among all extracts of whole plant of C. ciliata, the carbon tetrachloride soluble fraction of the methanolic extract showed strong cytotoxic activity. Aqueous soluble fraction of the methanolic extract, methanolic crude and ethyl acetate soluble fraction of the methanolic extract showed mild cytotoxity as compared to that of vincristine sulphate. The study confirms the mild to moderate antioxidant and moderate potent cytotoxic activities of C. ciliata plant extract as compared to reference standards and therefore demands the isolation of active principles and thorough bioassay.DOI: http://dx.doi.org/10.3329/icpj.v2i2.13196 International Current Pharmaceutical Journal 2013, 2(2): 38-41

scholarly journals Antimicrobial and Cytotoxic Activities of the Extracts of Glochidion multiloculare

2013 ◽  
Vol 10 (1) ◽  
pp. 1-11
Author(s):  
Selina Kabir ◽  
AM Sarwaruddin Chowdhury ◽  
Mohammad A Rashid ◽  
Choudhury M Hasan
Keyword(s):  
Escherichia Coli ◽  
Petroleum Ether ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Stem Bark ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Chloroform Fraction ◽  
Brine Shrimp Lethality ◽  
Ether Fraction

The current study was designed to investigate the antimicrobial and cytotoxic activities of methanol extract (MEGM), petroleum ether fraction (PEFGM), carbontetrachloride fraction (CTFGM), chloroform fraction (CFFGM) and aquous fraction (AQFGM) of stem bark of Glochidion multiloculare (Euphorbiaceae). Antimicrobial activity was evaluated by disc diffusion method and cytotoxic activity by brine shrimp lethality bioassay. In case of antimicrobial screening the CFFGM showed moderate inhibitory activity against B. subtilis having the zone size 12 mm and against Escherichia coli and Escherichia coli were 11 mm, while in the brine shrimp lethality bioassay, the petroleum ether soluble fraction revealed the highest cytotoxicity having LC50 of 3.11 g/ml. DOI: http://dx.doi.org/10.3329/jsf.v10i1.16273 J Sci Foundation, January-June 2012;10(1):1-11

scholarly journals Antioxidant, Antimicrobial and Cytotoxic Activities of Different Fractions of Citrus aurantifolia Peel

2020 ◽  
Vol 19 (2) ◽  
pp. 161-168
Author(s):  
Rupali Ghosh ◽  
Nazia Hoque ◽  
Meena Afroze Shanta ◽  
Nishat Nasrin ◽  
Muhammad Asaduzzaman
Keyword(s):  
Petroleum Ether ◽  
Brine Shrimp ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Brine Shrimp Lethality ◽  
Citrus Aurantifolia ◽  
Radical Scavenging Assay

The present study was designed to investigate antioxidant, antimicrobial and cytotoxic effects of petroleum ether, chloroform and hydromethanol fraction of methanol extract of Citrus aurantifolia peel. Preliminary phytochemical screening of the fractions was done following the standard procedure. Antioxidant activity was measured using DPPH free radical scavenging assay besides measuring total phenolic and flavonoid content using Folin-Ciocalteu reagent and aluminum trichloride method, respectively. The antimicrobial activity was conducted by disc diffusion method and cytotoxic activity was determined by brine shrimp lethality bioassay. The results of phytochemical screening were indicative of the presence of steroids, alkaloids, saponins, glycosides, flavonoids in the fractions. A dose dependent scavenging activity was observed in DPPH radical scavenging assay where chloroform fraction demonstrated the highest activity with IC50 value of 153.68 ± 3.60 μg/ml. The highest phenolic content was observed in chloroform fraction (308.0 ± 6.55 mg/g gallic acid equivalent) and highest flavonoid content was found in hydromethanol fraction (132.66 ± 2.36 mg/g quercetin equivalent). The chloroform fraction showed excellent antibacterial activity against all the tested bacteria where highest zone of inhibition (19 mm) was produced against Bacillus cereus. In brine shrimp lethality bioassay, LC50 values for petroleum ether, chloroform and hydromethanol fractions were 367.39 μg/ml, 228.14 μg/ml and 296.52 μg/ml, respectively. The present findings suggested that C. aurantifolia peel could be a potent source of medicinally important secondary metabolites and further investigations can be done to identify those active compounds responsible for such bioactivity. Dhaka Univ. J. Pharm. Sci. 19(2): 161-168, 2020 (December)

scholarly journals The Effect of Ethanol Extracted of Balsamina (Impatiens balsamina Linn) leaf on the Growth of Candida albicans In Vitro

2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Nadia Naitullah ◽  
Faisal Jamin ◽  
Frengki Frengki ◽  
Maryulia Dewi
Keyword(s):  
Candida Albicans ◽  
Crude Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Impatiens Balsamina ◽  
Inhibition Zones

This research aimed to determine the effect of ethanol extract of Impatiens balsamina Linn leaves on the growth of Candida albicans in vitro. One kilogram of Impatiens balsamina leaves which grows in Aceh was used. The leaves were washed, dried and blended into powder. The powder was then extracted with ethanol, filtrated, and evaporated. The crude extract was then made into serial concentration of 100, 75, 50, and 25%. The test of the effect of giving ethanol extract in Impatiens balsamina Linn is determined by the Kirby-Bauer disc diffusion method. Results showed that inhibition zones for 0, 25, 50, 75, and 100% were 0.0, 8.66±1.53, 11.66±1.55, 13.66±1.52, and 6±5.29 consecutively. It can be concluded that the ethanol extract of Impatiens balsamina Linn leaves± inhibit the growth of Candida albicans.Key words: Candida albicans, balsamina leaf, Impatiens balsamina Linn, extract

scholarly journals Chemical and Biological Investigation of Leaves of Polygonum plebejum

1970 ◽  
Vol 2 (2) ◽  
pp. 66-71 ◽  
Author(s):  
Md Kamrul Hasan ◽  
Abul Kalam Lutful Kabir ◽  
Sabyasachy Mistry
Keyword(s):  
Antimicrobial Activity ◽  
Spectral Analysis ◽  
13C Nmr ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Pharmaceutical Sciences ◽  
Disc Diffusion ◽  
Biological Investigation

The cytotoxic and antimicrobial activity of methanol crude extract and column fractions of the extract of theleaves of P. plebejum were examined by brine shrimp lethality bioassay and disc diffusion methodrespectively. The extracts showed significant cytotoxic as well as antimicrobial activities. Silica gel columnchromatography of methanol extract of P. plebejum afforded a steroid. The structure was elucidated on thebasis of spectral analysis, including 1H NMR and 13C NMR and also by comparing with data in the literature. Key words: Polygonum plebejum; Polygonaceae; Stigmasterol; Cytotoxicity.DOI: 10.3329/sjps.v2i2.5826Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 66-71

scholarly journals A simple disc diffusion method for detecting AmpC and extended-spectrum  -lactamases in clinical isolates of Enterobacteriaceae

2009 ◽  
Vol 63 (3) ◽  
pp. 497-501 ◽  
Author(s):  
H. Derbyshire ◽  
G. Kay ◽  
K. Evans ◽  
C. Vaughan ◽  
U. Kavuri ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Extended Spectrum
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