scholarly journals Effect of combined treatment with Methotrexate and Non-steroidal anti-inflammatory drugs on plasma alpha-tocopherol level and Rheumatoid factor among Rheumatoid Arthritis patients

Mediscope ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 80-86
Author(s):  
Farhana Pervin ◽  
Md Anwar Habib ◽  
Nazimuddin Ahmed ◽  
Md Mijanur Rahman Sardar
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Rheumatoid Factor ◽  
Combined Treatment ◽  
Alpha Tocopherol ◽  
Qualitative Assessment ◽  
Medical College ◽  
Tocopherol Level ◽  
Anti Oxidant ◽  
Inflammatory Drugs

Background: Oxidative stress might play a significant role in the pathogenesis of Rheumatoid Arthritis. Traditional therapies of Rheumatoid Arthritis are governed by different NSAIDs which improve symptoms of Rheumatoid Arthritis. But addition of Methotrexate (MTX) to Non-steroidal anti-inflammatory drugs (NSAIDs) has been found to be extra beneficial by halting the disease process. Objectives: To evaluate the status of anti-oxidant alpha-tocopherol and rheumatoid factor before and after treatment with MTX and NSAIDs for two months in Rheumatoid Arthritis patients. Methods: This quasi-experimental study was carried out in the Department of Pharmacology and Therapeutics of Rajshahi Medical College, Rajshahi between the periods of January 2011 to December 2011. Total ten clinically diagnosed Rheumatoid Arthritis patients were enrolled in the study. Purposive sampling technique was used to select each study subject from medicine wards of Rajshahi Medical College Hospital. Alpha-tocopherol, an anti-oxidant in plasma was measured as marker of anti-oxidant defense. The patients were then treated with oral MTX at a dose of 10 mg weekly and Indomethacin 150 mg in three divided doses daily for 2 months. After 2 months of continuous aforementioned treatment, alpha-tocopherol levels were estimated again. Moreover qualitative assessment of rheumatoid factor was done. For statistical analysis, paired t-test was done. Results: After two months of treatment with Methotrexate and Indomethacin, plasma alpha-tocopherol levels were significantly higher (P<0.05) in Rheumatoid Arthritis patients. However no significant change was observed in qualitative assessment of rheumatoid factors. Conclusion: From the findings of this study, it can be concluded that the endogenous alpha-tocopherol level increases even without any supplementation of alpha-tocopherol by combined treatment with MTX and Indomethacin in Rheumatoid Arthritis patients and thereby reduces oxidative stress. So alpha-tocopherol level is a better marker for early assessment of prognosis of Rheumatoid arthritis than Rheumatoid factor test. Mediscope 2021;8(2): 80-86

scholarly journals Risk Evaluation for the Formation of Gastroduodenal Erosion and Ulceration Induced by Meloxicam in Patients with Seropositive and Seronegative Rheumatoid Arthritis

2019 ◽  
Vol 29 (2) ◽  
pp. 45-52
Author(s):  
R. A. Pavlyukov ◽  
M. R. Konorev
Keyword(s):  
Rheumatoid Arthritis ◽  
Risk Factor ◽  
Rheumatoid Factor ◽  
Risk Evaluation ◽  
Prognostic Value ◽  
Patient Monitoring ◽  
Randomized Study ◽  
Gastroduodenal Erosion ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Aim. In this paper, the authors set out toascertain the prognostic value of the rheumatoid factor for the formation of gastroduodenal erosions and ulcers in patients with rheumatoid arthritis taking non-steroidal anti-inflammatory drugs (meloxicam).Materials and methods. A prospective, randomized study of 138 patients with rheumatoid arthritis taking methotrexate (12.5 mg per week) and meloxicam — nonsteroidal anti-inflammatory drug — (15 mg per day) was conducted. The formation of gastroduodenal erosions and ulcers was recorded for 4–8 years during esophagogastroduo-denoscopy (at study entry, following three months, then once a year). The groups of seropositive and seronegative patients comprised 69 people each.Results. During the period of patient monitoring, significant differences were found (P < 0.01) in the frequency of the formation of gastric and duodenal erosions and ulcers in patients with seropositive (79.7 %; 95 % CI: 70.2–89.2 %) and seronegative (4.4 %; 95 % CI: 0.01–9.2 %) rheumatoid arthritis (τ = 0.763; P < 0.01), taking meloxicam. In the course of monitoring patients (for 4–8 years) with rheumatoid arthritis who took meloxicam, the authors established high rates of the prognostic (rheumatoid) factor for predicting the formation of gastric and duodenal erosions and ulcers: forecast sensitivity — 94.8 %, forecast specificity — 82.5 %, the proportion of correct forecasts — 87.7 %.Conclusions. The rheumatoid factor is found to be a significant (P < 0.01) risk factor for gastroduodenal erosions and ulcers in patients with rheumatoid arthritis, who take non-steroidal anti-inflammatory drugs (meloxicam). 

scholarly journals Diclofenac Synthesis

2021 ◽  
Author(s):  
Aymen Labidi
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Hepatic Injury ◽  
Liver Toxicity ◽  
Reactive Intermediates ◽  
The United States ◽  
Hepatocellular Injury ◽  
Hepatocellular Damage ◽  
Immune Mediated ◽  
Inflammatory Drugs

Diclofenac is an NSAID, Non-Steroidal Anti-Inflammatory Drugs, widely used clinically in the treatement of osteoarthritis, rheumatoid arthritis, and ankylosing spondylitis, and has been available in the United States since 1988 (Banks et al. 1995). Diclofenac can cause hepatotoxicity, and despite the rare nature of these events, affects a large number of people due to its widespread usage. It is one of the most common drugs associated with idiosyncratic hepatic injury (Daly et al. 2007). From November 1988 to June 1991 the FDA received 180 cases of diclofenac-induced adverse hepatic injury of which 79% were female, 71% were elderly patients (above the age of 60), and 77% of the patients had osteoarthritis. In most of the cases the liver injury was characterized by hepatocellular damage or mixed hepatocellular injury, and there were some cases of cholestasis (Banks et al. 1995). The incidence of diclofenac hepatotoxicity has been reported to be 6.3 per 100 000 patients (DeAbajo et al. 2004). Bioactivation to reactive intermediates, their disposition, the production of oxidative stress, mitochondrial damage, and immune-mediated mechanisms have been suggested to play a role in diclofenac-mediated liver toxicity

scholarly journals Myeloperoxidase and Paraoxonase Activity in Acute Coronary Syndrome Patients

2021 ◽  
pp. 108-114
Author(s):  
Sowmya Varadhan ◽  
Ramesh Venkatachalam ◽  
A. Supriya Simon
Keyword(s):  
Oxidative Stress ◽  
Acute Coronary Syndrome ◽  
Oxidized Ldl ◽  
Predictive Marker ◽  
Significant Negative Correlation ◽  
Cross Sectional ◽  
Medical College ◽  
Coronary Syndrome ◽  
Anti Oxidant

Background: Myeloperoxidase (MPO), an oxidative stress related enzyme is elevated in Coronary Artery Disease (CAD) and is involved in development of atherosclerotic plaque. Paraoxonase (PON) an enzyme protein associated with HDL serves as an antioxidant and plays an important role in preventing the formation of Oxidized LDL (OxLDL). This suggests a conflicting role of MPO and PON in development of cardiovascular disease and atherosclerosis. Aim: Present study was done to evaluate and compare MPO/PON ratio in Acute Coronary Syndrome (ACS) patients with controls. The study evaluates and compares the pro oxidant and pro inflammatory enzyme, MPO and anti-oxidant and anti-inflammatory enzyme, PON in ACS patients with controls. Oxidative marker, Malondialdehyde (MDA) and anti-oxidant marker, Reduced Glutathione (GSH) was assessed in ACS patients and compared with controls. An attempt was also made to correlate MPO/PON ratio to markers of oxidative stress (MDA and GSH). Methods: Cross-sectional study carried out in Dr. Somervell Memorial CSI Medical College, Trivandrum, Kerala, India.50 ACS patients from Cardiac Care Unit and 50 age and sex matched controls without CAD from Medical College Health Checkup was selected. Results: MPO and MPO/PON ratio were significantly high and PON  was significantly lower in ACS patients compared to controls. Total cholesterol, Triglyceride, LDL, MDA were significantly high in ACS patients. Statistically significant positive correlation was observed between MPO/PON and MDA. Significant negative correlation was observed between MPO/PON and GSH. Conclusion: Myeloperoxidase and MPO/PON ratio was significantly high in ACS patients than controls. This is suggestive of the role of MPO in oxidative damage to lipoproteins in CAD patients. Prooxidant, Paraoxonase ,and antioxidant, GSH is lowered in ACS patients as a result of the increased oxidative stress. This study suggests that MPO/PON1 ratio can be used as a predictive marker of ACS.

scholarly journals Oxidative Stress and Antioxidant Status in Schizophrenia Patients

2016 ◽  
Vol 12 (2) ◽  
pp. 40-43
Author(s):  
Fatema Zerin Khan ◽  
Syeda Papia Sultana ◽  
Mohammad SI Mullick ◽  
Nargis Akhter
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Antioxidant Status ◽  
Armed Forces ◽  
Control Group ◽  
Study Group ◽  
Medical College ◽  
Anti Oxidant ◽  
Endogenous Antioxidants ◽  
Medical University

Introduction: Oxidative stress has been assumed to contribute to the pathophysiology of schizophrenia. Increased oxidative stress is the result of either an increased production of free radicals or a depletion of the endogenous antioxidants. Objective: To assess the levels of oxidative stress and antioxidant status in schizophrenia. Materials and Methods: This observational study was carried out in the department of Pharmacology, Bangabandhu Sheikh Mujib Medical University from September 2013 to January 2015. Ninety three schizophrenia patients were enrolled as study group and 30 healthy indivuduals were taken as control group. The peripheral levels of several molecules associated with oxidative stress namely malondialdehyde (MDA), glutathione (GSH) and anti-oxidant status like plasma levels of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) in 93 patients with schizophrenia and 30 healthy participants were assessed. Results: Study found that the schizophrenia group presented substantially higher levels of oxidative stress than the control group, as revealed by elevated quantities of the pro-oxidant MDA (6.3±0.5μmol/L in study group and 2.1±0.5μmol/L in control group), decreased levels of the antioxidants GSH (0.6±0.2mg/gm of Hb in study group and 2.1±0.5mg/gm of Hb in control group), plasma α-tocopherol and ascorbic acid. Results found were highly significant (p=0.001). Conclusion: In schizophrenia there are increased level of oxidative stress and decreased level of the antioxidants. Journal of Armed Forces Medical College Bangladesh Vol.12(2) 2016: 40-43

N-Acetylcysteine Attenuates the Anxiety-Like Behavior and Spatial Cognition Impairment Induced by Doxorubicin and Cyclophosphamide Combination Treatment in Rats

Pharmacology ◽  
2020 ◽  
pp. 1-8
Author(s):  
Yoshihisa Kitamura ◽  
Soichiro Ushio ◽  
Yusuke Sumiyoshi ◽  
Yudai Wada ◽  
Ikuko Miyazaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cognitive Impairment ◽  
Spatial Cognition ◽  
Combination Treatment ◽  
Combined Treatment ◽  
Cognitive Disorders ◽  
The Novel ◽  
Location Recognition ◽  
Anti Oxidant ◽  
Cognition Impairment

<b><i>Background:</i></b> Cancer patients can suffer from psychological and cognitive disorders after chemotherapy, which influence quality of life. <b><i>Objective:</i></b> Oxidative stress may contribute to the psychological and cognitive disorders induced in rats by chemotherapy. In the present study, we examined the effects of N-acetylcysteine, an anti-oxidant, on anxiety-like behavior and cognitive impairment in rats treated with a combination of doxorubicin and cyclophosphamide. <b><i>Methods:</i></b> Rats were intraperitoneally injected with doxorubicin and cyclophosphamide once a week for 2 weeks. The light-dark test and the novel location recognition test were used to assess anxiety-like behavior and spatial cognition, respectively. The rats’ hippocampal levels of glutathione (GSH) and glutathione disulfide (GSSG) were measured using a GSSG/GSH quantification kit. <b><i>Results:</i></b> Combined treatment with doxorubicin and cyclophosphamide produced anxiety-like behavior and cognitive impairment in rats. N-acetylcysteine reversed the anxiety-like behavior and inhibition of novel location recognition induced by the combination treatment. Furthermore, the combination of doxorubicin and cyclophosphamide significantly reduced the rats’ hippocampal GSH/GSSG ratios. N-acetylcysteine reversed the reduction in the GSH/GSSG ratio seen in the doxorubicin and cyclophosphamide-treated rats. <b><i>Conclusion:</i></b> These results suggest that N-acetylcysteine inhibits doxorubicin and cyclophosphamide-induced anxiety-like behavior and cognitive impairment by reducing oxidative stress in the hippocampus.

USE OF ANTI-CYCLIC CITRULLINATED PEPTIDE (ANTI-CCP) ANTIBODIES AND RHEUMATOID FACTOR (RF) IN DIAGNOSIS OF RHEUMATOID ARTHRITIS

2021 ◽  
pp. 67-70
Author(s):  
Rahul Ranjan ◽  
Md Shoeb Alam ◽  
V N Jha
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Treatment Decision ◽  
Pulmonary Involvement ◽  
Diagnostic Value ◽  
Cross Sectional Study ◽  
P Value ◽  
Cross Sectional ◽  
Medical College ◽  
Combined Use

INTRODUCTION: Rheumatoid arthritis (RA) is a chronic, systemic, inammatory autoimmune disease that initially affects small joints, progressing to larger joints, and eventually the skin, eyes, heart, kidneys, and lungs. This study was conducted to determine the usefulness of antiCCP antibodies and Rheumatoid Factor (RF) in diagnosis of Rheumatoid Arthritis and to estimate the diagnostic value of anti-CCP antibodies compared with Rheumatoid Factor (RF) in the diagnosis of Rheumatoid Arthritis. MATERIALS AND METHODS: This study was conducted in the Department of Medicine and it was a cross sectional study. A total of 86 patients presented with clinically suspected rheumatoid arthritis who attended the OPD Medicine were consecutively recruited. The present study was conducted on patients with clinically suspected rheumatoid arthritis from Department of Medicine, Darbhanga Medical College & Hospital, Laheriasarai, Bihar over a period of 12 months. RESULT: Anti-CCP antibody was not signicantly associated with the disease activity (p value =0.36). Anti-CCP antibody was signicantly associated with radiological defects whereby majority of patients with radiological defects (n=21/30; 70%) were positive for anti-CCPantibody (p value=0.03). Anti-CCP antibody was not signicantly associated with the incidence of rheumatoid nodule (p value =0.750). Anti-CCP antibody was not signicantly associated with extra-articular manifestation (p value =0.398). There is no signicant association between anti-CCPantibody and incidence of pulmonary involvement (p value =0.367). CONCLUSION: Combined use of RF and anti-CCP is a better prognostic and diagnostic tool than conventional RF tests alone. Uses of anti-CCP in clinical practice contribute to enhance the ability of rheumatologists to make judicious treatment decision. The usage of anti-CCP antibody is useful in the detection of early disease as evidenced by signicant association between anti-CCPantibody, RF, and radiological involvement in our study.

scholarly journals The common analgesic paracetamol enhances the anti-tumour activity of decitabine through exacerbation of oxidative stress

2020 ◽  
Author(s):  
Hannah J. Gleneadie ◽  
Amy H. Baker ◽  
Nikolaos Batis ◽  
Jennifer Bryant ◽  
Yao Jiang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Cancer Cell ◽  
Therapeutic Potential ◽  
Combined Treatment ◽  
Glutathione Depletion ◽  
Prostaglandin E ◽  
Cell Proliferation And Differentiation ◽  
Cancer Cell Survival ◽  
Anti Oxidant

AbstractThe DNA demethylating agent 5-aza-2’-deoxycytidine (DAC, decitabine) has anti-cancer therapeutic potential, but its clinical efficacy is hindered by DNA damage-related side effects. Here we describe how paracetamol augments the effects of DAC on cancer cell proliferation and differentiation, without enhancing DNA damage. Firstly, DAC specifically upregulates cyclooxygenase-2-prostaglandin E2 pathway, inadvertently increasing cancer cell survival, while the addition of paracetamol offsets this effect. Secondly, combined treatment leads to glutathione depletion and ROS accumulation with oxidative stress further enhanced by DAC suppressing anti-oxidant and thioredoxin responses. The benefits of combined treatment are demonstrated here in head and neck squamous cell carcinoma (HNSCC) and acute myeloid leukaemia cell lines, further corroborated in a HNSCC xenograft mouse model and through mining of publicly available DAC and paracetamol responses. In summary, the addition of paracetamol could allow for DAC dose reduction, widening its clinical usability and providing a strong rationale for consideration in cancer therapy.

scholarly journals Oxidative stress factors in Nigerians with rheumatoid arthritis

2017 ◽  
Vol 5 (5) ◽  
pp. 1948
Author(s):  
Yeldu M. H. ◽  
Arinola O. G.
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Rheumatoid Factor ◽  
Plasma Levels ◽  
Autoimmune Disorder ◽  
Stress Factors ◽  
Stress Index ◽  
Anthropometric Parameters ◽  
Total Antioxidant ◽  
Key Events

Background: Rheumatoid arthritis (RA) is a chronic progressive inflammatory autoimmune disorder characterized by symmetric erosive synovitis and sometimes with multi-system involvement. But the exact mechanism of the disease is not fully understood. In the light of above explanation, the present study measured the plasma levels of total peroxide potential (TPP), total antioxidant potential (TAP), malondialdehyde (MDA), oxidative stress index (OSI) and nitric oxide (NO) in relation to the titer of rheumatoid factor among RA patients compared with controls.Methods: This study included 28 rheumatoid arthritis patients and 28 apparently healthy subjects as controls who were matched for age (50-60 years), sex, and socioeconomic status. Rheumatoid factor was estimated using latex method as described by manufacturer. Anthropometric parameters and plasma levels of TPP, TAP, OSI, MDA and NO were determined using standard techniques.Results: The result indicated that with the exception of mean body weight which was significantly (p<0.001) higher among RA patients (90.61±2.02 years) as compared with controls (77.91±2.51 years), mean age, height and body mass index of RA patients (55.68±1.05kg, 1.65±0.01m and 33.40±0.83 kg/m2 respectively) were not significantly different compared with controls (54.07±1.04kg, 1.61±0.02m and 30.44±1.28 kg/m2 respectively). Plasma TPP, NO, OSI and MDA were significantly (p<0.01; p<0.001) higher while, plasma TAP is significantly lower among RA patients compared with controls. Plasma MDA was positively correlated with titer of rheumatoid factor in the RA patients.Conclusions: Our findings therefore may raise the concept that there are some yet unknown key events in the pathogenesis of RA determination of sex of the skull along with other parameters.

scholarly journals RHEUMATOID ARTHRITIS;

2019 ◽  
Vol 26 (02) ◽  
Author(s):  
Khadija Kiran ◽  
Uzma Jamil ◽  
Amtul Huda Sobhi ◽  
Zuhair Bhatti
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Cross Sectional Study ◽  
Categorical Variables ◽  
Control Group ◽  
P Value ◽  
Continuous Variables ◽  
Healthy Individuals ◽  
Cross Sectional ◽  
Medical College

Objectives: Objective of our study is to measure the concentration and role of Malondialdehydein determining the oxidative stress in rheumatoid arthritis patients in comparison with healthy individuals. Study Design: Cross sectional study. Setting: Department of physiology Gujranwala Medical College and Shalamar Medical College, Lahore. Period: From October 2016 to October 2017. Methodology: 180 patients of age 49 to 70 years of either gender. The approval for conducting experimental study was taken from ethical committee and consent information was taken from the patient on prescribed Performa. Data was analyzed on SPSS Version 23 related to study. Continuous variables were presented as mean and standard deviation like age and serum MDA concentration. Categorical variables were presented as numbers and percentages like gender. Post operative chi square test was applied and p- value less than or equal to 0.05 was considered as significant. Results: Total 180 person (n=180) of either genderwere included in this study. The study group was divided into two equal groups. Group A (RA group) consists of 90 patients (n=90) based on clinically and laboratory based criteria while group B (control group) consists of 90 (n=90) normal healthy individuals. The serum MDA levels in the RA patients were 3.97±1.03 nmoles/ml and it was 1.59±0.32 n moles/ml in control patients. The difference was statistically significant (t=20.87, p=0.001). Conclusion: The observations of our study showed statistically significant raised serum Malondialdehyde level as compared to control group which shows that raised serum MDA is an indicator of oxidative stress in rheumatoid arthritis patients.  

scholarly journals A study on gender related differences in oxidative stress status

Mediscope ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 13-18
Author(s):  
MMR Sardar ◽  
MOI Ali ◽  
F Pervin ◽  
F Yeasmin ◽  
N Ahmed ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxide ◽  
Alpha Tocopherol ◽  
Systematic Sampling ◽  
Female Group ◽  
Tocopherol Concentration ◽  
Medical College ◽  
Male Group ◽  
Oxidative Stress Status ◽  
The Mean

Female lives longer than male and incidence of cardiovascular disease is lower in young female. However the role of oxidative stress has been repeatedly described in various diseases but rarely healthy people. The objective of the study was to observe the gender related differences in oxidative stress status and to show significance in alteration. This cross sectional and comparative study was carried out in the Department of Physiology, Rajshahi Medical College during the period from January 2013 to June 2014. Total 45 healthy adult subjects (25 males and 20 females) were enrolled in the study. Systematic sampling technique was used to select each study subject from students, doctors and staffs of Rajshahi Medical College. Malondialdehyde (MDA), a lipid peroxide and alpha-tocopherol, an antioxidant in plasma was measured. MDA:alpha-tocopherol ratio was used as a reliable marker of oxidative stress status. The mean plasma alpha-tocopherol concentration was significantly higher (p < 0.05) and plasma MDA: alpha-tocopherol ratio was significantly lower (p < 0.05) in female group than male group. The mean plasma alpha-tocopherol concentration was significantly higher (p < 0.05) in female group aged 18-21 years than the corresponding male group. From the results of this study, it can be concluded that oxidative stress is less in female than in male indicating presence of potent compensatory response against oxidative stress in female which may be attributed to influence of female sex hormones. Mediscope Vol. 6, No. 1: Jan 2019, Page 13-18

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