scholarly journals Isolation and Identification of Bacterial Flora from Internal Organs of Broiler and Their Antibiogram Studies

2013 ◽  
Vol 1 (2) ◽  
pp. 72-75 ◽  
Author(s):  
Sampa Rani Roy ◽  
Md Bahanur Rahman ◽  
Jayedul Hassan ◽  
KHM Nazmul Hussain Nazir
Keyword(s):  
Bacterial Species ◽  
Bacterial Flora ◽  
Research Work ◽  
Easy Access ◽  
Sample Type ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Internal Organs ◽  
E Coli ◽  
Bacillus Spp

The present research work was carried out for the isolation and identification of bacterial flora from internal organs of broiler during the period from January 2012 to June 2012. Ten Hubbard classic broiler bird were purchased from retail market in Mymensingh, Bangladesh. The birds were sacrificed and their liver, lung, esophagus, duodenum and tracheal swab samples were collected (n=50). Using standard bacteriological techniques, Escherichia coli was isolated from 26 (52%) samples. Similarly, Salmonella spp., Staphylococcus spp., Bacillus spp., and Pasteurella spp. were isolated from 15 (30%), 10 (20%), 9 (18%) and 4 (8%) samples, respectively. On the basis of individual sample type, E. coli could be isolated from 8 (80%) duodenum samples being the most prevalent organism. On the other hand, Salmonella spp., Staphylococci spp., Bacillus spp. and Pasteurella spp. were identified in 5 (50%) lungs, 5 (50%) liver, 4 (40%) duodenum and 2 (20%) lungs samples, respectively. Among these isolated bacteria, E. coli was found to be pathogenic for mice. Antibiogram studies revealed that Ciprofloxacin was highly sensitive against all the isolated bacteria. Diversified bacterial species are prevalent in broiler. However, E. coli and Salmonella spp. infection might make the bird vulnerable for easy access of infection. Proper vaccination and use of selective antibiotics are crucial in protecting broilers from these pathogens. DOI: http://dx.doi.org/10.3329/mh.v1i2.14094 Microbes and Health, 2012 1(2): 72-75

scholarly journals Isolation and identification of bacterial pathogens from cloacal swabs of turkeys and their antimicrobial sensitivity patterns

2018 ◽  
Vol 3 (4) ◽  
pp. 419-425
Author(s):  
Polash Chandra Roy ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Md Shahin Mahmud ◽  
...  
Keyword(s):  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Research Work ◽  
Cloacal Swab ◽  
Easy Access ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Pass Through

The present study was carried for the isolation, identification of bacterial pathogens from cloacal swabs of turkeys during the period from January-June, 2016. The entire research work was conducted in the Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur. The study was performed with 48 cloacal swab samples. The cloacal swab samples were collected carefully from three different Turkey Farms randomly and transferred aseptically to the laboratory. On the basis of morphology, staining, cultural and biochemical characteristics it was found that among the isolates 25(52.08%) samples were positive E. coli, 10(20.83%) samples were positive for Salmonella spp., 9(18.76%) samples were positive for both E. coli and Salmonella spp. and 4(8.33%) samples shown no growth in subculture media. Antibiogram profiles indicate that E. coli isolated were 100% sensitive to Azithromycin, Kanamycin and Ciprofloxacin, 80% sensitive to Cefradine, Vancomycin and Levofloxacin, 60% sensitive to Cefotetan and Nitrofurantoin and 40% sensitive to Erythromycin. The isolates were 100% resistant to Cloxacillin and Cefixime. On the other hand, Salmonella spp. were 100% sensitive to Azithromycin, Kanamycin, Levofloxacin and Ciprofloxacin, 80% sensitive to Nitrofurantoin and Teicoplanin, 60% sensitive to Vancomycin, Erythromycin and Cefixime and 20% sensitive to Cefotetan. The isolates were 100% resistant to cefradine and cloxacillin. So, for E. coli Azithromycin, Kanamycin and Ciprofloxacin were more sensitive and for Salmonella spp. Azithromycin, Kanamycin, Ciprofloxacin and Levofloxacin were highly sensitive. Diversified bacterial species were present in cloacal swabs of Turkeys. However, E. coli, Salmonella spp. infection might make the birds vulnerable for easy access of infection. It could be concluded that E. coli and Salmonella spp. may pass through the feces to the environment. It causes a potential human health hazards and can cause illness.Asian J. Med. Biol. Res. December 2017, 3(4): 419-425

scholarly journals Isolation, molecular identification and antibiogram profiles of Escherichia coli and Salmonella spp. from diarrhoeic cattle reared in selected areas of Bangladesh

2017 ◽  
Vol 2 (4) ◽  
pp. 587-595
Author(s):  
M Sohidullah ◽  
Md Shahidur Rahman Khan ◽  
Md Shafiqul Islam ◽  
Md Mehedul Islam ◽  
Saifur Rahman ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Research Work ◽  
Clinical Signs ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli ◽  
Field Samples ◽  
Cultural Media

The present research work was undertaken to find out the passive causes of occurrences of diarrhoea in terms of age, sex, season and location differences through isolation and identification of the E. coli and Salmonella spp. using cultural, biochemical and molecular from the field samples of the diarrhoeic cattle and to study the antibiogram profiles of the isolated bacterial species. Considering above purposes, a total of 57 rectal swab samples were collected from the diarrhoeic cattle of Mymensingh sadar, Trishal, Valuka, Natore sadar and Gomostapur, Chapai Nawabganj. Different types of cultural media like Nutrient agar, MacConkey`s (MC) agar, Eosin Methylene Blue (EMB) agar, Salmonella-Shigella (SS) agar, Xylose-Lysine-Deoxycholate (XLD) agar and Blood agar were used to isolate and to study the cultural properties of the E. coli and Salmonella spp. Finally Gram’s staining and different biochemical tests were performed to identify those two bacterial species. Out of 57 samples, 27 were positive for E. coli and 8 were positive for Salmonella spp. On the basis of information from cattle owners and clinical signs the prevalence of diarrhoea was recorded as 30.99% and the pvalue was calculated as 0.001 (p<0.01) which was noted as highly significant. The prevalence percentages of the E. coli and Salmonella spp. were differed depending on different epidemiological parameters like age, sex, season and location. Moreover, the molecular identifications were further confirmed by means of PCR assay using specific primers for E. coli and Salmonella spp. This was done targeting 16S rRNA gene where they were found to be positive showing amplification of 585 bp for E. coli and 574 bp for Salmonella spp. From the study of the antibiogram profiles, it was revealed that E. coli were susceptible to ciprofloxacin, gentamicin and norfloxacin but resistant to tetracycline, erythromycin, amoxicillin and streptomycin whereas Salmonella spp. were susceptible to ciprofloxacin, gentamicin, amoxicillin and streptomycin but resistant to azithromycin, tetracycline and erythromycin. The findings of this research work would certainly help to select the proper antibiotics against diarrhoea in cattle of Bangladesh and to overcome the multi-drug resistant problem of the bacteria.Asian J. Med. Biol. Res. December 2016, 2(4): 587-595

scholarly journals ISOLATION AND IDENTIFICATION OF MICROFLORA FROM APPARENTLY HEALTHY CAGED PARROTS OF DHAKA ZOO OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 05-10 ◽  
Author(s):  
J Akhter ◽  
MT Hossain ◽  
MT Islam ◽  
MP Siddique ◽  
MA Islam
Keyword(s):  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Apparently Healthy

The research work was conducted to isolate and identify the microflora from apparently healthy caged parrots. A total of 45 samples (oral swabs, cloacal swabs and feces) were collected from five types of caged parrots (Gray cockatiels, Rose ringed parakeet, Alexandriane parakeet, Red breast parakeet and Blossom headed parakeet) of Dhaka Zoo during the period from April to August 2009. The samples were cultured on different bacteriological media and the bacteria were identified by their cultural and biochemical properties. All the isolates were allowed for antibiogram study. The bacteria isolated in this study from different types of caged parrots were E. coli (64.44%), Salmonella spp. (46.67%), Staphylococcus spp. (46.67%), Pasteurella spp. (33.33%), Proteus spp. (6.67%) and some unidentified Gram-positive and Gram-negative bacteria. Of these isolates, E. coli was the most frequent isolate. The frequency of Gram-negative bacteria was higher in this study. The percentage of bacterial isolates recovered from each type of parrots was almost similar. Irrespective of types of parrots, the higher percentage of different bacteria was isolated from cloacal swab (77.78%) followed by feces (75.56%). The 68.89% isolates were recovered from oral swab. All the suspected isolates of Salmonella spp. were confirmed by slide agglutination test using Salmonella polyvalent ‘O’ antiserum. Among the 21 Salmonella spp. isolated in this study, 4 (19.05%) isolates were identified as S. Pullorum when tested with specific antisera against S. Pullorum. The results of antibiotic sensitivity tests revealed that ampicillin and amoxicillin were completely resistant to E. coli and Pasteurella spp.; ampicillin to Proteus spp.; and furazolidone to Salmonella spp. and Pasteurella spp. However, the antibiotics of fluoroquinolone group such as ciprofloxacin, norfloxacin and enrofloxacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, ciprofloxacin was found to be consistently highly sensitive to all the bacterial isolates. DOI = 10.3329/bjvm.v8i1.8349 Bangl. J. Vet. Med. (2010). 8(1): 05-10

scholarly journals Isolation and Characterization of Microflora from Human Urine

2014 ◽  
Vol 2 (1) ◽  
pp. 15-18
Author(s):  
Kohinoor Parvin ◽  
Nahid Rahman ◽  
M Mostafizur Rahman ◽  
M Alimul Islam
Keyword(s):  
Escherichia Coli ◽  
Human Urine ◽  
Bacterial Flora ◽  
Research Work ◽  
Antibiotic Sensitivity ◽  
Diabetic Patients ◽  
E Coli ◽  
Isolation And Characterization ◽  
Bacillus Spp ◽  
Staphylococcus Spp

The research work was conducted to isolate & characterized the bacteria isolated from mid-stream urine of human. A total of 30 samples were collected from patients with urinary tract infection (n=10samples); diabetic patients (n=10samples) & apparently healthy individuals (n=10samples). Escherichia coli, Staphylococcus spp. and Bacillus spp. was the predominant bacterial flora of human urine. Among the isolates, the prevalence of Escherichia coli was highest (80%) compared to Staphylococcus spp.(14%) and Bacillus spp.(6%). E. coli isolated from 6 different sources were found to be highly virulent, moderately virulent, less virulent and avirulent categories as observed in day-old suckling mice. Antibiotic sensitivity profiles suggest that nalidixic acid will be the first drugs of choice to treat the UTI caused by E. coli and arythromycin, ampicillin and azithromycin will be the second drugs of choice to treat the UTI caused by Staphylococcus spp and Bacillus spp. respectively. Microbes and Health, June 2013, 2(1): 15-18DOI: http://dx.doi.org/10.3329/mh.v2i1.17257

scholarly journals Isolation and identification of bacterial flora from respiratory tract of healthy horses

2016 ◽  
Vol 13 (2) ◽  
pp. 239-246
Author(s):  
ZB Muktha ◽  
SML Kabir ◽  
MT Rahman
Keyword(s):  
Staphylococcus Aureus ◽  
Respiratory Tract ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Antibiotic Sensitivity ◽  
Aerobic Condition ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
E Coli

This study was carried out during the period of July to December, 2014 in order to isolate and characterize bacterial flora present in the respiratory tract of healthy horses in and around BAU campus. Eighteen apparently healthy horses were used for the study. Swab samples were collected from the nasal cavity. The bacteria was isolated ,identified and characterized by cultural (aerobic condition),staining, biochemical and PCR technique. Each of the samples collected yielded at least one bacterial species. A total of 27 bacteria were isolated from the selected animals. The majority of the isolates (15/27, 55.56%) were Gram-positive and the rest (12/27, 44.44%) were Gram-negative. Bacterial isolates were Staphylococcus aureus (83.33%) and E. coli (66.66%).The antimicrobial susceptibility pattern of bacterial isolates was investigated by disc diffusion method. The antibiotic sensitivity test of Staphylococcus aureus revealed that the isolates were highly sensitive to ciprofloxacin, moderately sensitive to gentamicin and resistance to amoxicilin, ampicilin and erythromycin. On the other hand, E. coli showed moderately sensitive to ciprofloxacin and gentamycin, mildly sensitive to erythromycin and resistance to amoxicilin and ampicilin. Detection of E. coli and S. aureus from the respiratory tract of healthy horses were not unexpected. Ciprofloxacin and gentamicin could be used for therapeutic purpose, if diseases occur by these organisms in horses.J. Bangladesh Agril. Univ. 13(2): 239-246, December 2015

scholarly journals Isolation and Identification of Bacterial Agents Causing Clinical Mastitis in Cattle in Mymensingh and Their Antibiogram Profile

2014 ◽  
Vol 2 (1) ◽  
pp. 19-21
Author(s):  
Md Tanvir Rahman ◽  
Md Shafiqul Islam ◽  
Mahmudul Hasan
Keyword(s):  
Dairy Cattle ◽  
Bacterial Species ◽  
Clinical Mastitis ◽  
Clinical Form ◽  
Isolation And Identification ◽  
E Coli ◽  
The World ◽  
Bacillus Spp ◽  
Microbial Etiology ◽  
Staphylococcus Spp

Mastitis is a serious problem for the dairy cattle in many countries of the world including Bangladesh. Among the microbial etiology bacteria plays a major role in the onset of the clinical form of the disease. Many of these bacteria are resistant to one or more antibiotics thus make the mastitis cases difficult to cure. In the present study Staphylococcus spp., Streptococcus spp., Bacillus spp. and E. coli were identified as the dominant bacterial species causing clinical mastitis in cattle in Mymensingh. Antibiogram study revealed chloramphenicol and erythromycin as the most effective antibiotic for the treatment of mastitis in these animals except those caused by Bacillus spp. and E. coli, respectively. Microbes and Health, June 2013, 2(1): 19-21DOI: http://dx.doi.org/10.3329/mh.v2i1.17258

scholarly journals Bacterial Flora of Koi (Anabas testudineus) Harvested from Ponds and Their Antibiogram

2014 ◽  
Vol 2 (1) ◽  
pp. 8-11
Author(s):  
Begum Safia Zaman ◽  
Mst Minara Khatun ◽  
Md Ariful Islam ◽  
Shadia Sharmin ◽  
Umme Kulsum ◽  
...  
Keyword(s):  
Water Samples ◽  
Bacterial Flora ◽  
Antibiotic Sensitivity ◽  
Bacterial Count ◽  
Plate Count ◽  
Salmonella Spp ◽  
Public Health Importance ◽  
Isolation And Identification ◽  
Anabas Testudineus ◽  
E Coli

Fish is known to harbour bacteria of public health importance. Aquatic environments of ponds are known to influence the bacterial loads of the harvested fish. The present work was undertaken to determine total viable count (TVC) and isolation and identification of bacteria from Koi fish, mud and water samples of two selected ponds managed and owned by the Bangladesh Fisheries Research Institute (BFRI) and two ponds located at Muktagacha, Mymensingh, managed by private owners. Fish (n=16), mud (n=16) and water (n=16) samples were collected from all 4 ponds. Samples were cultured on plate count agar to determine TVC. Fish harvested from Muktagacha ponds had statistically higher bacterial count (8.44 ± 0.04 log10 CFU/ml) when compared to BFRI ponds (7.92±0.17 log10 CFU/ml) (p? 0.05). Similarly, highest TVC was found in mud and water samples of Muktagacha ponds (6.87±0.73 and 7.41±0.04 log10 CFU/ml, respectively) compared to mud and water samples of BFRI ponds (5.04 ±0.07 and 5.40±0.09 log10 CFU/ml, respectively). Samples were inoculated onto appropriate selective media for isolation of bacteria. Total 257 bacterial isolates representing five genera were identified: Pseudomonas spp. (21.40%), Aeromonas spp. (33.46%), Vibrio spp. (14.78%), Salmonella spp. (21.40%) and E. coli (8.94%). Antibiotic sensitivity assay showed multidrug resistant profiles of Pseudomonas sp., Aeromonas sp., Salmonella sp. and E. coli. These bacteria are known to cause food borne illness in humans and spoilage of fish. Microbes and Health, June 2013, 2(1): 8-11DOI: http://dx.doi.org/10.3329/mh.v2i1.17255

scholarly journals Bacterial Flora Isolated From Dried Fishes Sold at Retail Markets Within Dhaka City Corporation of Bangladesh

2021 ◽  
Vol 8 (1) ◽  
pp. 109-115
Author(s):  
Md Kamrul Hassan ◽  
Munalisa Poppy ◽  
Tanvir Ahamed ◽  
Fahima Morsheda ◽  
Ravi Yadav ◽  
...  
Keyword(s):  
Bacterial Flora ◽  
Biochemical Properties ◽  
Detection Methods ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Retail Markets ◽  
Fish Samples ◽  
Bacillus Spp ◽  
Dried Fish ◽  
Staphylococcus Spp

Along with other contaminants, bacterial contamination in the dried fishes is a common issue which severely affects the quality of cured fishes. Hence, the present study was aimed to isolate and identify different bacterial flora contaminating different dried fishes, sold at different retail markets within Dhaka Metropolitan city, Dhaka, Bangladesh. A total of 25 different dried fishes were collected from Dhaka North city corporation area (Town Hall kacha bazar, Krishi market, and Mohammadpur kacha bazar) and Dhaka South city corporation area (New market kacha bazar, and Jatrabari chowrasta bazar). Each sample was divided into three regions (head, body, and tail) and bacterial isolation and identification was done by studying their cultural, staining and biochemical properties. Of the 25 dried fishes, Escherichia coli, Bacillus spp., Staphylococcus spp., Salmonella spp. and unidentified bacteria isolation rates were 44%, 56%, 80%, 48%, and 24%, respectively. The presence of E. coli and Salmonella spp. in various dried fish samples could be considered as indication of lack of hygienic condition during dried fish processing. Confirmation through molecular detection methods, pathogenicity, and antibiogram of the isolated bacteria could be included for future study. Res. Agric., Livest. Fish.8(1): 109-115, April 2021

scholarly journals Microbiological quality of commercially available poultry feeds sold in Bangladesh

2017 ◽  
Vol 3 (1) ◽  
pp. 52-60
Author(s):  
Nigar Sultana ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Mst Deloara Begum ◽  
...  
Keyword(s):  
Public Health ◽  
Methylene Blue ◽  
Culture Media ◽  
Microbiological Quality ◽  
Control Measures ◽  
Nutrient Agar ◽  
Poultry Feed ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli

The study was conducted aiming at the isolation and identification of pathogens from poultry feed manufactured by four different brands namely A (broiler starter), B (broiler finisher), C (layer starter) and D (layer finisher) sold in retail stores of Rangpur city of Bangladesh. All these samples were collected from four randomly chosen outlets and analyzed by culturing in different culture media such as Nutrient broth (NB), Nutrient agar (NA), Salmonella-Shigella (SS) agar, Eosin methylene blue (EMB) agar, MacConkey agar, Triple sugar iron (TSI) agar slant, Motility, Indole, Urease (MIU) and Saboraud Dextrose agar (SDA) media. The bacterial agents were isolated and examined under light microscope for their gross morphological and conventional biochemical characteristics. The bacteriological analyses were done at the Microbiology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period of January to June, 2014. Total bacterial colonies of all the samples were counted separately according to the American Public Health Association, using nutrient agar medium for total viable count (TVC), Eosine methylene blue (EMB) agar media for total E. coli count (TEC) and Salmonella-Shigella agar for TSC (total salmonella count). Saboraud Dextrose agar (SDA) media was used for detection of fungus. The virulence effect of the organism present in feed were observed by inoculating the organism in poultry. Recorded result showed that average TVC of feed sample A, B, C and D were 5.45x106, 3.28x105, 5.14x106 and 4.53x105 CFU/gm (colony forming unit per gram) respectively. TEC of feed sample A, B, C and D were recorded 6.25x105, 8.26x103, 5.52x105 and 5.65x104 CFU/gm respectively. TSC of feed sample A, B, C and D were recorded 3.15x104, 2.68x103, 4.46x103 and 1.19x104 CFU/gm respectively. The highest TVC, TEC and TSC were found in broiler starter (feed sample A) and lowest TVC, TEC and TSC were found in broiler finisher (feed sample B). Fungal count was 1.85x105 CFU/ gm in layer finisher (feed sample D) could be as a result of their high pathogenecity as reported by researchers elsewhere. These organisms can cause several poultry and farm animal infections specially mycotoxicosis having public health significance to both human and poultry. The presence of high numbers of E. coli and Salmonella spp. in poultry feed were indicative of poor hygienic practices during manufacture, post process contamination and unsatisfactory transportation and reservation. Therefore reinforce the need for preventive control measures, hygienic handling and processing of feeds to reduce the risk of potential human health hazards.Asian J. Med. Biol. Res. March 2017, 3(1): 52-60

A STUDY OF CROSS-CONTAMINATION OF FOODBORNE PATHOGENS ON THE KITCHEN SURFACES

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Murni Noor Al Amin ◽  
Wan Rosmiza Zana Wan Dagang
Keyword(s):  
Foodborne Pathogens ◽  
Cross Contamination ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli ◽  
Meal Preparation ◽  
Before And After ◽  
Change In The Mean ◽  
Plate Counts ◽  
Raw Poultry

Cross-contamination provides the opportunity for various of bacteria to be deposited on each of the surface contact during meal preparation. Raw poultry especially raw chicken was the main reservoir of foodborne pathogens that can cause foodborne diseases. Therefore, a study on the potential of cross-contamination contribute to spread E. coli, Salmonella spp. and S. aureus on the kitchen surfaces during chicken preparation was conducted. A total of 36 isolates were collected from six sampling sites before and after the chicken preparation. The enumeration of the bacteria from the sampling sites showed a significant change in the mean total plate counts (TPC) of the isolates before and after the chicken preparation. These results emphasized that cross-contamination occurred around the sampling sites during the preparation of the chicken. Isolation and identification of the three foodborne pathogens, E. coli, Salmonella spp. and S. aureus were carried out on its respectively selective and differential media. The presumptive identified foodborne pathogens were confirmed as E. coli, Salmonella spp. and S. aureus according to their microscopic and biochemical characteristics.

Sign in / Sign up

Export Citation Format

Share Document