scholarly journals Isolation and Characterization of Bacterial Species from Enteric Fever Suspected Human Cases of Mymensingh Medical College Hospital

2013 ◽  
Vol 20 (1-2) ◽  
pp. 85-92
Author(s):  
M Al-Amin ◽  
MSR Khan ◽  
F Begum ◽  
J Hasan ◽  
M Asaduzzaman
Keyword(s):  
Escherichia Coli ◽  
Enteric Fever ◽  
Bacterial Species ◽  
Medical College Hospital ◽  
Pathogenicity Test ◽  
College Hospital ◽  
Medical College ◽  
Isolation And Characterization ◽  
Stool Samples ◽  
Klebsiella Spp

A total of 17 stool samples were collected from enteric fever suspected patients of Mymensingh Medical College Hospital, Mymensingh, Bangladesh. Results of morphological, biochemical examinations and pathogenicity test revealed Escherichia coli (47%), Klebsiella spp. (41%), Citrobacter spp. (29%). In cultural examination, all Escherichia coli showed metallic sheen on EMB agar, Klebsiella spp. showed moist shiny mucoid colonies and Citrobacter spp. showed large convex colorless dwarf colonies on MacConkey agar. In biochemical examination, all the isolates fermented five basic sugars with the production of acid and gas where the Citrobacter spp. were late lactose fermenter. Escherichia coli was indole and MR positive but V-P negative, Klebsiella spp. were V-P positive but MR and Indole negative and Citrobacter spp. were MR positive but V-P and Indole negative. All isolated bacterial species were sensitive to ciprofloxacin but resistant to amoxicillin. Salmonella typhi or Salmonella paratyphi organisms were not present in the stool samples examined. This may be due to collection of samples from patient having antibiotic therapy with Ceftriaxone through intravenous route for 3 days prior to collection of samples. From this study, it may be concluded that Escherichia coli, Kelbsiella spp. and Citrobacter spp. may be commonly present in enteric fever suspected patients or they may produce enteric fever like symptoms in human.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16857 Progress. Agric. 20(1 & 2): 85 – 92, 2009  

Antibiotic Sensitivity and Resistance Patterns of Salmonella Typhi in Rajshahi Medical College Hospital

2020 ◽  
Vol 33 (2) ◽  
pp. 10-14
Author(s):  
Md Azizul Haque ◽  
Laila Shamima Sharmin ◽  
KM Faisal Alam ◽  
Md Mohimanul Hoque ◽  
M Morsed Zaman Miah ◽  
...  
Keyword(s):  
Blood Culture ◽  
Enteric Fever ◽  
Antibiotic Sensitivity ◽  
Salmonella Typhi ◽  
Medical College Hospital ◽  
Personal Hygiene ◽  
Care Hospital ◽  
College Hospital ◽  
Medical College ◽  
Sensitivity Pattern

Typhoid and paratyphoid fevers, collectively known as enteric fever, is caused by Salmonella enterica subspecies serovars Typhi and Paratyphi A, B and C. Despite this declining global trend, enteric fever is still considered to be a major public health hazard in Bangladesh and other developing countries due to poor sanitation, inadequate food safety measures and poor personal hygiene. In Bangladesh, the incidence of typhoid fever was reported to be 200 episodes per 100,000 person-years during 2003–2004. Multidrug-resistant (resistance to the first-line antimicrobials ampicillin, cotrimoxazole, and chloramphenicol) strains of S. Typhi and S. Paratyphi are on the rise globally and even cases of extensively drug-resistant (XDR) typhoid cases resistant to chloramphenicol, ampicillin, trimethoprim-sulfamethoxazole, third generation cephalosporins and fluoroquinolones are being reported from many corners of the world. This descriptive, observational study was carried out in Rajshahi Medical College Hospital Hospital, Rajshahi, Bangladesh from July 2017 to June 2019. Antibiotic sensitivity pattern of total 76 cases of enteric fever due to Salmonella Typhi were studied. Blood culture was carried out by BACT ALERT-3D, Automated blood culture analyzer from BioMeriuex SA, France Patented FAN Plus method. Based on the minimum inhibitory concentration (MIC), the organism was categorized as sensitive, intermediate, and resistant against the respective antibiotics as per Clinical and Laboratory Standards Institute (CLSI) criteria. We are reporting antibiotic sensitivity and resistant patterns of S. Typhi documented in Rajshahi Medical College Hospital, a large tertiary care hospital in Northern Bangladesh. TAJ 2020; 33(2): 10-14

scholarly journals Infection of protozoan and Helminth parasites among the out-patients of Dhaka Medical College Hospital

2016 ◽  
Vol 44 (1) ◽  
pp. 89-97
Author(s):  
Hamida Khanum ◽  
Ainun Nahar ◽  
Mt Tahmina Karim ◽  
Hasina Banu
Keyword(s):  
Ascaris Lumbricoides ◽  
Intestinal Parasites ◽  
Medical College Hospital ◽  
Intestinal Helminth ◽  
Helminth Parasites ◽  
Enterobius Vermicularis ◽  
College Hospital ◽  
Medical College ◽  
Ancylostoma Duodenale ◽  
Stool Samples

The intestinal parasites and their prevalence and intensity of infestation was investigated. A total of 793 stool samples were examined from the out patients of Dhaka medical College Hospital. Eggs and lavae of six species of intestinal parasites were recovered, of which two species were protozoans: Entamoeba histolytica Schaudinn, 1903; and Giardia intestinalis (Lambl, 1859) and four species of helminthes: Ascaris lumbricoides Linnaeus, 1758; Trichuris trichiura (Linnaeus, 1771) Stiles, 1901; Ancylostoma duodenale (Dubini, 1843) Creplin, 1845; and Enterobius vermicularis (Linnaeus, 1758)]. The overall prevalence of infestation was 43.253% and egg per gram (EPG) of infestation was slightly higher in males than females. Prevalence of intestinal helminth was found higher than intestinal protozoans. Among the six species of intestinal parasites, Ascaris lumbricoides showed highest prevalence (21.689%) and Enterobius vermicularis lowest prevalence (1.008%). Among the patients of the hospital, highest prevalence (83.333%) was observed in 0 - 10 years age group and lowest (30.167%) in 51 - 60 years age-group.Bangladesh J. Zool. 44(1): 89-97, 2016

scholarly journals Isolation and Antimicrobial Susceptibility Pattern of Urinary Escherichia Coli in Dhaka Medical College Hospital, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 40-44
Author(s):  
Tashmin Afroz Binte Islam ◽  
SM Shamsuzzaman ◽  
Irin Rahman ◽  
Faria Ferdousi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Test Reaction ◽  
College Hospital ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

Background: Escherichia coli is the major bacterial pathogen being isolated and reported from urine, globally. For these uropathogens the therapeutic management becomes limited, so knowledge about the antimicrobial resistance pattern of these agents at a specific area may help the doctors to choose correct treatment regimen. The aim of the current study was to detect the common uropathogens from urine and the antimicrobial susceptibility pattern of Escherichia coli in Dhaka Medical College Hospital (DMCH).Methods: Samples were collected over a period of 12 months from July 2011 to June 2012 from the patients of DMCH irrespective of age and sex. Samples were cultured in blood agar and MacConkey agar media and organisms were identified by different biochemical tests such as oxidase test, reaction in MIU and simmon's citrate media and different sugar fermentation tests. Data were analyzed using Microsoft Excel (2007).Results: From total 300 urine samples, 177 (59%) Gram-positive and Gram-negative bacteria were isolated. Among them, Esch. coli was the most predominant 112 (63.28%) organism followed by Enterobacter spp. (11.87%). The prevalence of Esch. coli was significantly higher in females (63.39%) of younger age group than in males (36.61%). Esch. coli was highly sensitive to imipenem (100%) followed by ceftriaxone (62.50%), ceftazidime (55.36%) and azitromycin (35.72%) respectively. Low sensitivity patterns were found against doxycycline (13.39%), co-trimoxazole (16.07%), amoxiclav (21.43%) and ciprofloxacin (23.22%) respectively.Conclusion: The result of this study provides insight into the high proportion of multidrug resistant Escherichia coli and creates a need to report them routinely in laboratories.Anwer Khan Modern Medical College Journal Vol. 7, No. 1: Jan 2016, P 40-44

scholarly journals Prevalence and Antibiogram of Microbial Agents Causing Nosocomial Urinary Tract Infection in Surgical Ward of Dhaka Medical College Hospital

2016 ◽  
Vol 6 (2) ◽  
pp. 75-79
Author(s):  
Tashmin Afroz Binte Islam ◽  
SM Shamsuzzaman ◽  
Naznin Nehar ◽  
Jannatul Fardows
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infections ◽  
Medical College Hospital ◽  
High Rate ◽  
Structural Constraints ◽  
College Hospital ◽  
Medical College ◽  
Esbl Producers

Background: Nosocomial infections pose substantial risk to patients receiving care in hospitals. In Bangladesh, this problem is aggravated by inadequate infection control due to poor hygiene, resource and structural constraints and lack of awareness regarding nosocomial infections.Objective: We carried out this study to determine the prevalence of different microorganisms from urine in surgery ward and antimicrobial susceptibility pattern against various antibiotics.Materials and Methods: This cross sectional study was carried out in Department of Microbiology, Dhaka Medical College, Dhaka over a period of 12 months from July 2011 to June 2012. A total of 52 urine specimens were collected from catheterized patients admitted in general surgery ward of Dhaka Medical College Hospital (DMCH) and incubated in blood agar, MacConkey agar media and the isolates were identified by different biochemical tests – oxidase test and reaction in MIU (motility indole urease) and Simmon’s citrate and TSI (triple sugar iron) media. ESBL producers were detected by double-disk synergy test (DDST).Results: Bacteria were isolated from 35 specimens and Escherichia coli was the commonest isolate (23, 65.71%) followed by Pseudomonas aeruginosa 6 (17.14%), Klebsiella pneumoniae 3 (8.57%), Acinetobacter baumannii 2 (5.72%) and Proteus vulgaris 1 (2.86%) respectively. Among the isolates, 10 (28.57%) ESBL producers were detected and the highest ESBL production was observed in Escherichia coli (8, 22.85%) followed by Klebsiella pneumoniae 1 (2.86%) and Pseudomonas aeruginosa 1 (2.86%). The isolates were resistant to most of the commonly used antimicrobial agents.Conclusion: The emergence of multi-drug resistant (MDR) bacteria poses a difficult task for physicians who have limited therapeutic options. However, the high rate of nosocomial infections and multi-resistant pathogens necessitate urgent comprehensive interventions of infection control.J Enam Med Col 2016; 6(2): 75-79

scholarly journals A study of Serodiagnostic Challenges in Enteric Fever Cases Presenting as Co-infection with Dengue and Malaria

2018 ◽  
Vol 29 (Number 2) ◽  
pp. 3-7
Author(s):  
S K Saha ◽  
S Kabir ◽  
S N Ahmad ◽  
M A Eva
Keyword(s):  
Enteric Fever ◽  
Signs And Symptoms ◽  
Cross Sectional Study ◽  
Medical College Hospital ◽  
Public Health Importance ◽  
College Hospital ◽  
Cross Sectional ◽  
Female Population ◽  
Medical College ◽  
The Tropics

Enteric fever, Dengue and malaria still remain diseases of public health importance in the tropics. The aim of the study to see the serodiagnostic challenges in enteric fever cases presenting as co-infection with dengue and malaria. The cross sectional study was conducted in the department of Medicine and Department of Microbiology, Holy Family Red Crescent Medical College Hospital, from July 2016 to June 2017. Febrile patients attending the outpatient department or admitted to hospitals attached to Holy Family Red Crescent Medical College Hospital suspected to have signs and symptoms clinically suggestive of typhoid, malaria and/or dengue were initially enrolled. Seropositivity of typhoid-dengue it was observed that 55 (55.0%) patients had dengue IgM+typhoid, 27 (27.0%) had dengue IgG+typhoid, 10(10.0%) had dengue IgM+IgG+typhoid and 08(8.0%) were negative. Seropositivity of typhoid-malaria of the patients, it was observed that p. falciparum+typhoid was found in 33 (33.0%), p. vivax+typhoid was 22 (22.0%) and negative was 45 (45.0%). Male patients while co-infection with typhoid fever observed more than in female population. In the present study Seropositivity of typhoid-dengue fever is high of co-infections are common than typhoid-malaria, hence it should be properly diagnosed and timely medication should be provided to reduce complications and get proper treatment.

scholarly journals Prevalence and antibiogram of ESBL producing gram negative bacilli isolated from urine in Dhaka Medical College Hospital, Bangladesh

2017 ◽  
Vol 9 (1) ◽  
pp. 17-21
Author(s):  
Tashmin Afroz Binte Islam ◽  
S M Shamsuzzaman ◽  
Aleya Farzana
Keyword(s):  
Escherichia Coli ◽  
Treatment Strategies ◽  
Enterobacter Aerogenes ◽  
Medical College Hospital ◽  
Biochemical Tests ◽  
Test Reaction ◽  
College Hospital ◽  
Gram Negative ◽  
Medical College ◽  
Esbl Producers

Extended spectrum ß-lactamase (ESBL) producing strains are resistant to a wide variety of common antimicrobials and become a major clinical concern worldwide that has complicated treatment strategies. The current study has been carried out to detect ESBL producing gram negative bacilli with their antimicrobial susceptibility pattern from urine collected over a period of 12 months from July 2011 to June 2012 from Dhaka Medical College Hospital. Samples were cultured in blood agar and MacConkey's agar media and organisms were identified by different biochemical tests such as oxidase test, reaction in MIU and simmon's citrate media and different sugar fermentation tests. ESBL producers were detected by double-disk synergy test (DDST). From total of 300 urine samples, 157 (52.33%) gram negative bacilli causing UTI were isolated and most of them were Escherichia coli (71.34%) followed by Enterobacter aerogenes (13.38%). Among the isolates, 45 (28.66%) ESBL producers were detected. The highest of ESBL producer was observed in Escherichia coli (32.14%) followed by Klebsiella pneumoniae (28.57%), Enterobacter aerogenes (23.80%) and Acinetobacter spp (20%). ESBL producers were significantly more resistant to ciprofloxacin, gentamycin, doxycycline, amoxiclav and co-trimoxazole than non-ESBL producers. All the ESBL producing strains were sensitive to imipenem. The result of this study provides insight into the high proportion of highly resistant ESBL producing organisms and more effective strategies are needed to control the spread of these resistant organisms.Bangladesh J Med Microbiol 2015; 9 (1): 17-21

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

Chest Trauma Evaluation and Outcome of Management in a Tertiary Hospital - One Year Experience

2020 ◽  
Vol 22 (2) ◽  
pp. 110-117
Author(s):  
Md Mahmudul Islam ◽  
Khondkar AK Azad ◽  
Md Aminul Islam ◽  
Rivu Raj Chakraborty
Keyword(s):  
Road Traffic Accident ◽  
Traffic Accident ◽  
Chest Trauma ◽  
Road Traffic ◽  
Tube Thoracostomy ◽  
Rib Fracture ◽  
Medical College Hospital ◽  
College Hospital ◽  
Medical College ◽  
The Mean

Background: Chest trauma is responsible for 50% of deaths due to trauma. This kind of death usually occurs immediately after the trauma has occurred. Various therapeutic options have been reported for management of chest injuries like clinical observation, thoracocentesis, tube thoracostomy and open thoracotomy. Objective: To observe the pattern and outcome of management in chest trauma Methods: This is an observational study carried out in Casualty department of Chittagong Medical College Hospital (CMCH), Chittagong, between April 2015 to March 2016. Our study was included all patients, both sexes, following chest injury at Casualty units of Chittagong Medical College Hospital. All the data were recorded through the preformed data collection sheet and analyzed. Result: The mean age was found 37.7±18.1 years with range from 12 to 80 years. Male female ratio was 11.8:1. The mean time elapsed after trauma was found 6.1±3.1 hours with range from 1 to 72 hours. Almost one third (35.7%) patients was affecting road traffic accident followed by 42(27.3%) assault, 35(22.7%) stab injury, 15(9.7%) fall and 7(4.5%) gun shot . More than three fourth (80.5%) patients were managed by tube thoracostomy followed by 28(18.2%) observation and 2(1.3%) ventilatory support. No thoracotomy was done in emergency department. 42(27.2%) patients was found open pneumothorax followed by 41(26.6%) rib fracture, 31(20.1%) haemopneumothorax, 14(9%) simple pneumothorax, 12(7.8%) haemothorax, 6(3.9%) chest wall injury, 5(3.2%) tension pneumothorax, and 3(1.9%) flail chest. About the side of tube 60(39.0%) patients were given tube on left side followed by 57(37.0%) patients on right side, 9(5.8%) patients on both (left & right) side and 28(18.2%) patients needed no tube. Regarding the complications, 13(30%) patients had persistent haemothorax followed by 12(29%)tubes were placed outside triangle of safety, 6(13.9%) tubes were kinked, 6(13.9%) patients developed port side infection, 2(4.5%)tube was placed too shallow, 2(4.5%) patients developed empyema thoracis and 2(4.5%) patients developed bronchopleural fistula. The mean ICT removal information was found 8.8±3.6 days with range from 4 to 18 days. Reinsertion of ICT was done in 6(4.7%) patients. More than two third (68.2%) patients were recovered well, 43(27.9%) patients developed complication and 6(3.9%)patients died. More than two third (66.9%) patients had length of hospital stay 11-20 days. Conclusion: Most of the patients were in 3rd decade and male predominant. Road traffic accident and tube thoracostomy were more common. Open pneumothorax, rib fracture and haemopneumothorax were commonest injuries. Nearly one third of the patients had developed complications. Re-insertion of ICT needed almost five percent and death almost four percent. Journal of Surgical Sciences (2018) Vol. 22 (2) : 110-117

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