scholarly journals Determination of carboxyhaemoglobin and methaemoglobin levels in donated blood

2021 ◽  
Vol 10 (10) ◽  
pp. e294101018859
Author(s):  
Alessandra Cristina Pupin Silvério ◽  
Simone Caetani Machado ◽  
Vanessa Caroline Cardoso Silva ◽  
Estéfane Rodrigues ◽  
Isarita Martins
Keyword(s):  
Storage Period ◽  
University Hospital ◽  
Donor Blood ◽  
Spectrophotometric Methods ◽  
Collecting Ducts ◽  
Blood Banks ◽  
Before And After ◽  
Donated Blood ◽  
Citrate Phosphate

To determine the percentages of carboxyhaemoglobin (COHb) and methaemoglobin (MeHb) in donor blood and to compare these levels between smokers and nonsmokers at different time points during blood storage. Blood donors were recruited from Haematology Service, University Hospital Alzira Velano, Alfenas-MG. The blood was kept in collecting ducts (noodles) containing citrate, phosphate and dextrose (CPD) and stored at 4°C throughout the storage period. Since the noodles kept the characteristics of the bags, COHb and MeHb levels were analysed on the day of donation and after 20 days of storage. Levels of COHb and MeHb were determined using spectrophotometric methods. Non-parametric Friedman and Mann-Whitney tests were employed to compare COHb and MeHb levels before and after the storage and groups of smokers and nonsmokers, respectively. Levels of COHb and MeHb in the blood collected from smokers and nonsmokers were statistically different (p< 0.05; Mann- Whitney test) when the samples were analyzed before the storage. In blood of smokers, COHb levels were no different over a 20-day storage period (p= 0.7009; Friedman test). On the other hand, MeHb levels were significant different over a 20-day storage period (p< 0.05). The results suggest the need to regularly assess COHb and MeHb levels in donor blood stored in blood banks.

scholarly journals Platelet Counts in Stored Donor Blood

1982 ◽  
Vol 10 (3) ◽  
pp. 271-273 ◽  
Author(s):  
W. J. Russell ◽  
L. J. Tunbridge
Keyword(s):  
Blood Platelet ◽  
Platelet Activity ◽  
Normal Range ◽  
Donor Blood ◽  
Platelet Counts ◽  
Before And After ◽  
The Mean ◽  
Clinically Significant ◽  
The One ◽  
Citrate Phosphate

Platelets were counted in eight units each of one- and three-day-old blood. Counts were done both before and after the blood had passed through a standard 170 micron filter. In the one-day-old blood, platelet counts were within the normal range. The mean count was 237,000 platelets/microlitre. Platelet counts on three-day-old blood were lower, but generally still within the normal range. The mean count was 183,000/microlitre. Only a few platelets were retained by the filter in the transfusion set; about 90% of platelets passed the filter in both the one- and three-day-old blood. It appears that whole blood, anticoagulated with citrate/phosphate/dextrose (CPD), and stored under Australian blood bank conditions retains platelets in sufficient numbers for at least the first three days to be clinically significant. However, it remains to be determined whether satisfactory platelet activity can be expected during this time.

scholarly journals EFFECTIVENESS OF PROSTHODONTIC TREATMENT OF PATIENTS WITH INCREASED DENTAL ATTRITION ON THE BASIS OF FUNCTIONAL DIAGNOSTIC

2018 ◽  
Vol 22 (6) ◽  
pp. 297-300
Author(s):  
Alexander S. Romanov ◽  
V. G Morozov ◽  
P. N Geletin
Keyword(s):  
Dental Treatment ◽  
University Hospital ◽  
Control Group ◽  
Dental Attrition ◽  
Before And After ◽  
Medical University ◽  
Methods Of Treatment ◽  
Functional Diagnostic ◽  
Group 1

Aim of the present study was to evaluate the effectiveness of prosthodontic methods of treatment based of functional diagnostic of patients with increased dental attrition Methods. We have examined 35 patients at the Smolensk State Medical University Hospital: 20 patients with increased dental attrition and 15 patients who didn’t have any sign of functional disorders of dentoalveolar and musculoskeletal system. The main group was divided into two subgroups. In the group 1 (11 patients) required a prosthodontic therapy included individual wax-up of future prosthetic construction based on data of functional diagnostic. An electromyographic study was conducted before and one month after treatment for determination of the effectiveness of treatment methods. Results. Analyzing and comparing the data of the electromyographic study 1 month before and after treatment of patients of the 1 group have shown 93,1 % effectiveness of the treatment and the control group. The results of the study show that such occlusion is optimal for the muscles and temporomandibular joint. Conclusion. Dental treatment of increased attrition of teeth based on functional diagnostic contributes to the normalization of muscles activity. Moreover it allows to protect teeth from attrition and providing effective rehabilitation of patients.

scholarly journals Evaluation of the caseinomacropeptide (CMP) content in raw and uht milk during storage time.

2013 ◽  
Vol 2 (2) ◽  
Author(s):  
Elaine Closs ◽  
Claucia Fernanda Volken de Souza
Keyword(s):  
Storage Time ◽  
Chromatographic Method ◽  
Correlation Coefficients ◽  
Storage Period ◽  
Raw Milk ◽  
Bacteria Growth ◽  
Uht Milk ◽  
Psychrotrophic Bacteria ◽  
Spectrophotometric Methods

<p>Coolers are used in raw milk storage in order to inhibit the growth of lactic acid producer mesophilic microorganisms. However, refrigerated milk is a favorable environment to psychrotrophic aerobic bacteria growth. These psychrotrophic bacteria promote proteolysis which is of concern to the dairy industry since it may indicate a false positive of fraudulent addition of whey to raw milk. This study evaluated the influence of storage time on the presence of caseinmacropeptide (CMP) in raw milk during storage as well as in ultra-high temperature milk (UHT) throughout its shelf life. Three different determination methods were compared each other, namely the standard chromatographic method and two spectroscopy methods for the quantitative determination of sialic acid. One is based on the determination of ninhydrin acid and the other one is an adapted method using the Ehrlich reagent. An increase of CMP content throughout the storage period for the cooled raw milk and UHT milk was detected despite the fact that none had cheese whey. The spectrophotometric methods showed correlation coefficients greater than 0.97 with the chromatographic method established by Brazilian legislation. The results indicate that the spectrophotometric method using the ninhydrin acid can be used as an alternative method for the determination of CMP in milk samples.</p><p>&nbsp;</p><p>DOI: http://dx.doi.org/10.14685/rebrapa.v2i2.54</p>

scholarly journals PENGARUH WAKTU PENYIMPANAN DARAH TERHADAP KADAR HEMOGLOBIN PADA KOMPONEN WHOLE BLOOD DARAH DONOR

2021 ◽  
Vol 6 (2) ◽  
pp. 50-56
Author(s):  
Arief Adi Saputro ◽  
Catur Retno Lestari
Keyword(s):  
Blood Transfusion ◽  
Whole Blood ◽  
Storage Period ◽  
Blood Components ◽  
Donor Blood ◽  
Average Value ◽  
Significant Difference ◽  
Before And After ◽  
The Difference ◽  
First In First Out

Blood transfusion is the process of distributing blood from donors to patients with the aim of replacing blood lost due to bleeding, surgery, shock and malfunctioning of the blood-forming organs that require replacement blood in the form of whole blood or blood components. Blood is stored with the First in First Out (FIFO) system, which is a system that regulates the expulsion of blood in which the first blood that enters will be removed first. The storage period of blood will experience changes in blood components, especially erythrocytes will experience significant changes in shape over the length of time blood storage. The purpose of this study was to determine the difference in hemoglobin levels of donor blood before and after storage for 7 days. This research was conducted at the Blood Transfusion Unit of PMI Kudus Regency. The number of samples used as many as 15 samples. The results showed that there were differences in hemoglobin levels before storage and after storage for 7 days (0.000). The average value before storage was 14.7 g/dl, after storage for 7 days 18.2 g/dl, the highest hemoglobin before storage was 15.4 g/dl, the highest after storage was 18.2 g/dl, while the lowest hemoglobin was before storage. storage 14.0 gr/dl and after storage 17.3 gr/dl. The conclusion is there is a significant difference between hemoglobin levels before storage and after storage for 7 days.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

The Plasmin Inhibitors of Plasma

1964 ◽  
Vol 12 (01) ◽  
pp. 119-125 ◽  
Author(s):  
Y Shamash ◽  
A Rimon
Keyword(s):  
Human Plasma ◽  
New Method ◽  
Normal Amount ◽  
Caseinolytic Activity ◽  
Before And After ◽  
Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

FRACTIONAL DETERMINATIONS OF URINARY 17-KETOSTEROIDS IN HYPEREMESIS GRAVIDARUM

1962 ◽  
Vol 41 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Pentti A. Järvinen ◽  
Sykkö Pesonen ◽  
Pirkko Väänänen
Keyword(s):  
Hyperemesis Gravidarum ◽  
First Trimester ◽  
Control Group ◽  
Before And After ◽  
Daily Urine ◽  
First Trimester Of Pregnancy ◽  
The Difference

ABSTRACT The fractional determination of 17-ketosteroids in the daily urine was performed in nine cases of hyperemesis gravidarum and in four control cases, in the first trimester of pregnancy both before and after corticotrophin administration. The excretion of total 17-KS is similar in the two groups. Only in the hyperemesis group does the excretion of total 17-KS increase significantly after corticotrophin administration. The fractional determination reveals no difference between the two groups of patients with regard to the values of the fractions U (unidentified 17-KS), A (androsterone) and Rest (11-oxygenated 17-KS). The excretion of dehydroepiandrosterone is significantly higher in the hyperemesis group than in the control group. The excretion of androstanolone seems to be lower in the hyperemesis group than in the control group, but the difference is not statistically significant. The differences in the correlation between dehydroepiandrosterone and androstanolone in the two groups is significant. The high excretion of dehydroepiandrosterone and low excretion of androstanolone in cases of hyperemesis gravidarum is a sign of adrenal dysfunction.

DETERMINATION OF BLOOD CORTICOIDS USING THE IN VITRO RESIN UPTAKE OF 3H-PREDNISOLONE

1968 ◽  
Vol 57 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Hironori Nakajima ◽  
Mitsunori Murala ◽  
Masumitsu Nakata ◽  
Takeshi Naruse ◽  
Seiji Kubo
Keyword(s):  
Thyroid Function Test ◽  
Normal Subjects ◽  
Adrenal Function ◽  
Function Test ◽  
Before And After ◽  
Adrenal Response ◽  
Suppression Test

ABSTRACT The in vitro resin uptake of 3H-prednisolone was used for the determination of blood cortisol after addition of radioactive prednisolone followed by Amberlite CG 400 Type 1 to the test serum, and incubation of the mixture. The radioactivity of the supernatant was compared before and after the addition of the resin. The principle of this method is similar to that of the 131I-triiodothyronine resin uptake for the thyroid function test. The tests for the specificity, reproducibility and sensitivity gave satisfactory results. The mean basal value ± SD of the 3H-prednisolone resin uptake was 35.3 ± 9.2% in normal subjects, and 27.1 ± 4.8% in pregnant women. This method was valid in various adrenal function tests, i. e. the adrenal circadian rhythm, corticotrophin (ACTH) test, dexamethasone suppression test and the adrenal response to lysine-8-vasopressin. It proved to be a sensitive indicator of the adrenal function. These results suggest that this method should be useful for a routine adrenal function test.

GESTOGENS IN CORPUS LUTEUM OF CATTLE

1960 ◽  
Vol XXXIII (III) ◽  
pp. 417-427 ◽  
Author(s):  
John Kristoffersen
Keyword(s):  
Corpus Luteum ◽  
Average Value ◽  
Spectrophotometric Methods ◽  
The Third ◽  
Quantitative Measurements ◽  
Before And After ◽  
Luteal Tissue ◽  
In Pregnancy

ABSTRACT By means of chromatographic and spectrophotometric methods progesterone and a substance closely similar to 20β-hydroxy-pregn-4-ene-3-one has been detected in luteal tissue from non-pregnant and pregnant cows. In 21 animals quantitative measurements based on a method giving an average net recovery of 56 per cent showed that in pregnancy the average progesterone content in the corpus luteum reached a maximum in the third to fifth month, with low values before and after this period. For 6 nonpregnant cows, the average value was 20.2 μg/g tissue, which is considerably higher than previous values reported in the literature. The relation between these findings and the bovine dependence on a functional corpus luteum in pregnancy is discussed, and it is pointed out that more information about the metabolism of progesterone in cattle is highly desirable.

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

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