scholarly journals Male Swiss mice (Mus musculus) as a most suitable experimental model for the study of Giardia duodenalis BIV

2021 ◽  
Vol 10 (10) ◽  
pp. e493101019250
Author(s):  
Liara Izabela Lopes Romera ◽  
Renata Coltro Bezagio ◽  
Willian Costa Ferreira ◽  
Caroline Rodrigues de Almeida ◽  
Mônica Lúcia Gomes
Keyword(s):  
Experimental Model ◽  
Mus Musculus ◽  
Behavioral Assessment ◽  
Giardia Duodenalis ◽  
Negative Control ◽  
Feed Water ◽  
Clinical Parameters ◽  
Swiss Mice ◽  
Suitable Animal Model ◽  
Human Giardiasis

In this study, we proposed to verify the most suitable murine experimental model for studying human giardiasis. In total 150 animals were used. Fifty mice (Mus musculus) from each lineage (Swiss, Balb/c and C57BL/6), 25 females and 25 males, were divided into 5 groups with 5 animals each, according to the lineage/sex. Three groups were infected with 104 cysts of Giardia duodenalis of assemblage BIV and 2 negative control groups. The animals were followed and evaluated for 15 days after receiving the inoculum. The clinical parameters evaluated were body weight, water and feed intake, excretion, appearance of fur and feces, elimination of Giardia spp cysts and behavioral assessment. The clinical parameters of the groups infected with G. duodenalis were compared with the non-infected groups within their own lineage/sex. In the 15 days of monitoring, only the male Swiss mice presented differences in these parameters. The infected animals consumed more feed, water and eliminated more excreta than the non-infected group. There was no difference in the general average of the weight of the animals or in the behavioral assessment in any group. Only the infected male Swiss mice eliminated G. duodenalis cysts in the feces, which was confirmed by the molecular diagnosis and by observing the presence of trophozoites in the intestinal mucosa. The results demonstrate that the most suitable animal model for the study of human giardiasis is the male Swiss mice, since it was the only one capable of developing infection by G. duodenalis cysts.

scholarly journals UJI EFEK ANALGESIK EKSTRAK ETANOL KELOPAK BUNGA MATAHARI (Helianthus annuus Linn.) PADA MENCIT SWISS (Mus musculus)

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Citra R. Irianty ◽  
Jimmy Posangi ◽  
P. M. Wowor
Keyword(s):  
Helianthus Annuus ◽  
Mus Musculus ◽  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Group Iii ◽  
Helianthus Annuus L ◽  
Swiss Mice ◽  
Group I

Abstrak: Penggunaan tanaman obat semakin berkembang luas di masyarakat, salah satunya yaitu bunga matahari (Helianthus annuus L.). Tumbuhan ini dipercaya memiliki berbagai khasiat, diantaranya sebagai analgesik. Penelitian ini bertujuan untuk mengetahui efek analgesik dari ekstrak etanol kelopak bunga matahari (Helianthus annuus L.) pada mencit Swiss (Mus musculus). Penelitian ini merupakan penelitian eksperimental dengan menggunakan metode rangsangan panas berupa suhu 55°C. Pada penelitian ini digunakan hewan uji berupa mencit jantan yang dibagi dalam 3 kelompok masing-masing terdiri dari 3 ekor. Kelompok I kontrol negatif diberi Aquades 0,3 ml, kelompok II kontrol positif diberi Tramadol 0,18 mg/20grBB mencit dan kelompok III perlakuan diberi ekstrak etanol kelopak bunga matahari 0,3 ml untuk tiap mencit. Penelitian dilakukan selama 1 menit dengan menilai respon mencit berupa meloncat dan atau menjilat kakinya sebagai reaksi untuk mengurangi nyeri. Dari hasil percobaaan dengan pemberian rangsang panas berupa suhu 55°C menunjukkan bahwa respon mencit yang diberikan 0,3 ml ekstrak etanol kelopak bunga matahari, mulai menurun di menit ke-30 dan mencapai maksimal pada menit ke-90. Dari hasil penelitian ini didapatkan bahwa ekstrak etanol kelopak bunga matahari (Helianthus annuus L.) memiliki efek analgesik terhadap mencit Swiss.     Abstract: Sunflower (Helianthus annuus L.) is one of medical herbs where the use of medical plants is getting growth on social community. Those plants believed has many efficacious, one of them was as  analgesic. The aim of this research is to know the analgesic effect of ethanol extract of sunflowers petals (Helianthus annuus L.) in Swiss mice (Mus musculus). This study is an experimental study using heat stimuli of 55°C. This study used male mice which were divided into 3 groups each consisting of three mice. Group I was given distilled water and act as negative control which were given 0,3 ml, group II as positive control were given  0,18 mg/20grBB mice of Tramadol and the group III were given sunflower petals ethanol extract of 0,3 ml. An observation was carried out for 1 minute with expexted response being jumping and licking as a reaction to reduce pain. Results obtained by appling heat stimuli in the temperature of 55°C showed that sunflower petals ethanol extract in the dosase of 0,3 ml of ethanol extract sunflowers petals, start to decline in the 30th minute and reached a maximum in the 90th minute. From the result of this study found that ethanol extract of sunflower petals (Helianthus annuus L.) has an analgesic effect on Swiss mice.

PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

BioScience ◽  
2018 ◽  
Vol 2 (1) ◽  
pp. 61
Author(s):  
Elsa Yuniarti
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Negative Control ◽  
State University ◽  
Betel Leaf ◽  
Randomized Design ◽  
The Subject ◽  
Effective Drugs ◽  
Zoology Department

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

scholarly journals PENGARUH TRITERPEN TOTAL PEGAGAN (Centella asiatica (L) Urban) TERHADAP FUNGSI KOGNITIF BELAJAR DAN MENGINGAT PADA MENCIT JANTAN ALBINO (Mus musculus) YANG DIHAMBAT DENGAN SKOPOLAMIN

Molekul ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. 89
Author(s):  
Herlina Herlina
Keyword(s):  
Cognitive Function ◽  
Learning And Memory ◽  
Mus Musculus ◽  
Memory Performance ◽  
Centella Asiatica ◽  
Positive Control ◽  
Negative Control ◽  
Learning Ability ◽  
Control Group ◽  
Albino Mice

Pegagan (Centella asiatica (L) Urban) has been described to posses CNS effects such as improving cognitive function, learning and memory. The aim of the research was to evaluate the effects of total triterpen’s pegagan extract on cognitive functions as the learning and memory performance in male albino mice (Mus musculus) inhibited by scopolamine. The research design was Complete Randomized Design (RAL) – factorial on thirty six mice divided into 4 groups. One control group received only aquabidest (negative control). Three treatment groups received total triterpen 16 mg/kg BW, 32 mg/kg BW orally and piracetam 500 mg/kg BW by intra peritoneally (positive control) for 21 days. Data indicating learning and memory process of all subjects were obtained from one-trial passive avoidance test. Data were analyzed by two way ANOVA and BNT (p<0,05). Result showed that total triterpen 32 mg/kg BW had significantly prolonged the retention time compared to control group indicating improvement in cognitive function (505,03 second vs -18,53 second) (p<0,05) and it was not significantly different to piracetam 500 mg/kg BW group (505,03 second vs 522,48 second) (p>0,05). In conclusion, total triterpen from pegagan (Centella asiatica (L) Urban) improved learning ability and memory of male albino mice (Mus musculus) even though, it was inhibited by scopolamine.

Effect of simple and complex enrichment added to standard-sized cages in behavioral, physiological, and neurological variables in female Swiss mice (Mus musculus).

2021 ◽  
Vol 135 (3) ◽  
pp. 380-388
Author(s):  
Agustina Resasco ◽  
Rocío B. Foltran ◽  
Cecilia Carbone ◽  
Silvina L. Diaz
Keyword(s):  
Mus Musculus ◽  
Swiss Mice

scholarly journals Uji Aktivitas Ekstrak Biji Caesalpinia Sp terhadap Jumlah Eritrosit Mus musculus yang Diinfeksi Plasmodium berghei serta Implementasinya dalam Pembelajaran Kimia

2018 ◽  
Vol 2 (2) ◽  
pp. 196-199
Author(s):  
Nurleli Nurleli ◽  
Agus Sundaryono ◽  
Aceng Ruyani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Active Compounds ◽  
Chemistry Learning ◽  
Layer Chromatography

AbstraCT[Activity test of Caesalpinia sp seed extract on erythrocytes amount of Mus musculus infected by Plasmodium berghei and its implementation in chemistry learning]. The aims of this study were to (1) determine the activity of seed extract (Caesalpinia sp) on the number of Erythrocytes of M. Musculus of Swiss Webster Strain infected by P.berghei, (2) Find  the active compounds obtained from the isolation of Caesalpinia sp seed extract (3) Find the differences in student learning outcomes about the separation of secondary metabolites using thin layer chromatography (TLC) which is taught in chemistry learning by using LKS. In this study Caesalpinia sp seed extract was purified by thin layer chromatography and column coromatography, and was identified by IR and 1H-NMR spectroscopy. M. musculus infected with P. berghei was given Caesalpinia sp seed extract at a dose of 0.028 g/Kgbb, 0.056 g/Kgbb, and 0.084 g / Kgbb by gavage, while the positive control group was given Kloroquin diphosphate, negative control was given olive oil. Caesalpinia sp seed extract with a dose of 0.056 g/Kgbb inhibited the highest plasmodium, which was 42.89% and even exceeded the malaria drug commonly used chloroquin diphosphate 29.85%. Caesalpinia sp seed extract capable of reducing plasmodium in erythrocytes is also able to increase the number of uninfected erythrocytes. The active compounds obtained from the isolation of Caesalpinia sp seed extract are alkaloids, flavonoids, saponins, and terpenoids. In the implementation of extracurricular group learning is taught by using worksheets. There are significant differences where the average posttest score is 88.67 and the average pretest is 32.1. Keywords: Caesalpinia Sp.; Erythrocyte; Plasmodium berghei; Mus muscullus; Student working sheet.

scholarly journals Uji Aktivitas Ekstrak Biji Caesalpinia Sp terhadap Jumlah Eritrosit Mus musculus yang Diinfeksi Plasmodium berghei serta Implementasinya dalam Pembelajaran Kimia

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Nurleli Nurleli ◽  
Agus Sundaryono ◽  
Aceng Ruyani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mus Musculus ◽  
Plasmodium Berghei ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Active Compounds ◽  
Chemistry Learning ◽  
Layer Chromatography

AbstraCT[Activity test of Caesalpinia sp seed extract on erythrocytes amount of Mus musculus infected by Plasmodium berghei and its implementation in chemistry learning]. The aims of this study were to (1) determine the activity of seed extract (Caesalpinia sp) on the number of Erythrocytes of M. Musculus of Swiss Webster Strain infected by P.berghei, (2) Find  the active compounds obtained from the isolation of Caesalpinia sp seed extract (3) Find the differences in student learning outcomes about the separation of secondary metabolites using thin layer chromatography (TLC) which is taught in chemistry learning by using LKS. In this study Caesalpinia sp seed extract was purified by thin layer chromatography and column coromatography, and was identified by IR and 1H-NMR spectroscopy. M. musculus infected with P. berghei was given Caesalpinia sp seed extract at a dose of 0.028 g/Kgbb, 0.056 g/Kgbb, and 0.084 g / Kgbb by gavage, while the positive control group was given Kloroquin diphosphate, negative control was given olive oil. Caesalpinia sp seed extract with a dose of 0.056 g/Kgbb inhibited the highest plasmodium, which was 42.89% and even exceeded the malaria drug commonly used chloroquin diphosphate 29.85%. Caesalpinia sp seed extract capable of reducing plasmodium in erythrocytes is also able to increase the number of uninfected erythrocytes. The active compounds obtained from the isolation of Caesalpinia sp seed extract are alkaloids, flavonoids, saponins, and terpenoids. In the implementation of extracurricular group learning is taught by using worksheets. There are significant differences where the average posttest score is 88.67 and the average pretest is 32.1. Keywords: Caesalpinia Sp.; Erythrocyte; Plasmodium berghei; Mus muscullus; Student working sheet.

scholarly journals Efek Analgesik Ekstrak Etanolik Daun Mangrove Api-Api Putih (Avicennia alba) terhadap Mencit (Mus musculus L.) Jantan yang Diinduksi Asam Asetat 0,7%

2020 ◽  
Vol 18 (1) ◽  
pp. 68
Author(s):  
ANDREW THEO WILLIAM CHRIS PURMATA ◽  
LESTARI DEWI ◽  
CHOESNAN EFFENDI
Keyword(s):  
Acetic Acid ◽  
Traditional Medicine ◽  
Mus Musculus ◽  
Analgesic Effect ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Mangrove Species ◽  
Avicennia Alba

<p><strong>Background </strong>: <em>Avicennia alba </em>or known as mangrove api-api putih<em> </em>is a type of mangrove species which has many benefits and often to be used by the local Indonesians as traditional medicine. The alcaloid and flavonoid substances derived from <em>Avicennia alba </em>leaves<em> </em>could inhibit pain. This study aims to determine the analgesic effect of <em>Avicennia alba </em>leaves  extract in male mice (<em>Mus musculus L.</em>) induced by acetic acid 0,7%.<strong></strong></p><p class="Default"><strong>Method </strong>:25 mice were divided into 5 groups that were given different therapies. The therapy are 10 mL/kg bw of water, 150 mg/kg bw of acetosal, 250 mg/kg bw; 500mg/kg bw; 1000 mg/kg bw of <em>Avicennia alba </em>leaves extract. 0,7% acetic acid with dose of  10 mL/kg bw is given to each subject intraperitoneally 30 minutes after the therapy to induce pain. The pain is manifested as writhes in mice, the writhes then are counted repeatedly each 10 minutes for 30 minutes.</p><p class="Default"><strong>Result</strong>:<strong>:  </strong>mean of writhes in group treated with <em>Avicennia alba </em>leaves extract in 250 mg/kg bw and 500 mg/kg bw with each value 6,20 and 9,40 are less than mean of writhes in negative control group with value 31,40. %inhibition of <em>Avicennia alba </em>leaves extract with dose of 250 mg/kg bw and 500 mg/kg bw respectively are 80,25% and 70,06%.</p><p><strong>Conclusion </strong>: <em>Avicennia alba </em>leaves extract in 250 mg/kg bw and 500 mg/kg bw dosage showed analgesic effect in male mice (<em>Mus musculus L</em>.) induced with 0,7% acetic acid.</p><p><strong>Keywords </strong>:pain, analgesic, <em>Avicennia alba</em></p>

scholarly journals ANTIDIABETIC ACTIVITY FROM TABLETS EFFERVESCENT EXTRACT OF MIMOSA PUDICA LINN LEAVES

2020 ◽  
Vol 6 (2) ◽  
pp. 99-107
Author(s):  
Na'ilatul Azizah ◽  
Ika Buana Januarti ◽  
Annisa Masithoh ◽  
Anna Khoirun Nisa
Keyword(s):  
Mus Musculus ◽  
Negative Control ◽  
Antidiabetic Activity ◽  
Mimosa Pudica ◽  
Antidiabetic Treatment ◽  
Body Weight Data ◽  
Group I ◽  
Study Results ◽  
Significant Difference ◽  
Uniformity Test

The community has trusted mimosa pudica linn as an alternative antidiabetic treatment. Compounds that have antidiabetic activity in Mimosa pudica linn are flavonoids, alkaloids, and tannins. The selection of effervescent tablet dosage formulations is practical for diabetics with the addition of stevia leaf sweeteners. This study aimed to determine the antidiabetic activity of effervescent tablets of Mimosa pudica linn leaves extract as a natural antidiabetic. The research method begins with the extraction of plants using the maceration method of 70% ethanol; the tablet formulation uses dry granulation. Antidiabetic activity test using 28 male mice (Mus musculus) Swiss strain divided into four groups, namely group I (negative control): Na-CMC 0.5%, group II: effervescent tablets of Mimosa pudica Linn leaves Extract dose 600 mg/kgBB, group III (positive control): metformin 65 mg/kgBB, group IV (normal control): feed and drink. Each group, except normal controls, was induced by DM using alloxan 150 mg/kg body weight. Data on decreased blood glucose levels were analyzed using the Kruskal-Wallis statistical test the study results show that effervescent tablets meet the parameters of disperse time test, pH test, size uniformity test, weight uniformity test, hardness test, moisture test, and friability test. This study concludes is that there is a significant difference between the negative, positive, normal, and treatment control groups, which means that the administration of effervescent tablets of 70% ethanol extract of Mimosa pudica Linn leaves at a dose of 600 mg/kgBB can reduce blood sugar levels in mice (Mus musculus).

scholarly journals Mus musculus (Linnaeus, 1758) Immune Responses Caused by Escherichia coli (Migula, 1895) Infection

2017 ◽  
Vol 3 (4) ◽  
pp. 285
Author(s):  
Zuni Mitasari ◽  
Abdul Gofur ◽  
Dwi Listyorini
Keyword(s):  
Escherichia Coli ◽  
Tract Infection ◽  
Immune Responses ◽  
Mus Musculus ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Spleen Weight ◽  
E Coli ◽  
Carl Linnaeus

<p><em>Escherichia coli</em> (<a title="Walter Migula" href="https://en.wikipedia.org/wiki/Walter_Migula">Migula</a>, 1895) is a negative gram bacteria which have an ability to cause some deseases, such as meningitis, urinary tract infection and digestive tract infection. Lipopolysaccharide (LPS) in its cell wall induces immune responses in many ways. The objective of this study was to investigate the effect of <em>E. coli</em> infection on lymphocyte numbers and spleen weight in mice [<em>Mus musculus</em> (Linnaeus, 1758)]. Twenty five male mice were grouped into negative control, positive control group treated with PBS, and three experimental groups injected intraperitonially with <em>E. coli</em> 1.5 × 10<sup>3 </sup>cfu/mL,1.5 × 10<sup>5 </sup>cfu/mL or 1.5 × 10<sup>7 </sup>cfu/mL respectively.  About 5 d after the injection, the spleen were collected. Spleen were weighed using digital balance, number of lymphocyte were counted using hemocytometer. <em>E. coli</em> infection significantly increase both weight of the spleen and number lymphocyte. In conclusion,  mice responded to <em>E. coli</em> infection by increasing the number of its lymphocyte.</p><div><p class="Els-keywords"><em> </em></p><p class="Els-keywords"><strong>Keywords:</strong><em> Escerechia coli </em>(<a title="Walter Migula" href="https://en.wikipedia.org/wiki/Walter_Migula">Migula</a>, 1895);<em> </em>immune respone; infection; <em>Mus musculus </em>(<a title="Carl Linnaeus" href="https://en.wikipedia.org/wiki/Carl_Linnaeus">Linnaeus</a>, <a title="10th edition of Systema Naturae" href="https://en.wikipedia.org/wiki/10th_edition_of_Systema_Naturae">1758</a>).</p></div>

scholarly journals Efektivitas ekstrak metanol batang pisang mauli 100%padapenyembuhan luka punggung mencit (Mus musculus)ditinjau dari jumlah sel radang (Effectiveness of 100%extract of Mauli Banana stem to healing process of mice’s (Mus musculus)back from the perspective of the number of inflamed cell)

2014 ◽  
Vol 13 (1) ◽  
pp. 33
Author(s):  
Maharani Laillyza Apriasari ◽  
Amy Nindia Carabelly ◽  
Gusti Febby Aprilia
Keyword(s):  
Mus Musculus ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Banana Plant ◽  
Significant Difference ◽  
Stem Extract ◽  
Banana Stem

Banana plant especially its trunk contains many kinds of fitokimia such as saponin, flavonoid, and tannin whichfunction as antibiotic and fasten the healing process. Mauli banana stem extract is often used by the people in HuluSungai Utara Banjarmasin Province to fasten healing skin injury. The purpose of this research is to histopathologicallyexamine the effectiveness of Mauli Banana stem extract to the healing process of the mice’s back (Mus musculus)onthe 3rd day. This purely true experimental research with post test only with control design used 27 mices which weredivided to 3 groups. Treatment group was given methanol extract of mauli banana stem whose average scores ofneutrofil and macrofag are representatively 2.333 and 1; negative control group was given aquadest with its averageneutrofil and macrofag scores are representatively 1.055 dan 1.678; and positive control group was given standardmedicine which contained Aloe veraextract with its average neutrofil and macrofag scores are representatively 1.066dan 1.667. The result of Kruskall-Wallis and Mann-Whiney showed a significant difference between treatment groupand negative control group. It can be concluded that the extract methanol of mauli banana stem on the healing processof injury to mancit’s back histopathologically influences the decreasing acute inflamed (neutrofil) cell and theincreasing chronic inflamed (macrophage) cell on the third day. In this case the extract of mauli banana stem ismedically potential to fasten the healing process of injury.

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