scholarly journals Life-threatening reaction of a pediatric cancer patient to sodium hypochlorite

2021 ◽  
Vol 10 (13) ◽  
pp. e282101320446
Author(s):  
Bruna da Fonseca Wastner ◽  
Mariana de Souza Lessa ◽  
Laurindo Moacir Sassi ◽  
Mara Albonei Dudeque Pianovski
Keyword(s):  
Sodium Hypochlorite ◽  
Pediatric Cancer ◽  
Severe Reaction ◽  
Safety Measures ◽  
Lack Of Information ◽  
Special Cases ◽  
Cutaneous Rash ◽  
Immunosuppressed Patients ◽  
Life Threatening ◽  
Irrigation Solution

The use of sodium hypochlorite (NaOCl) as irrigation solution in endodontics is widespread and accidents of apical extravasation may occur, being in most cases mild to moderate situations of simple management. However, in immunosuppressed patients, the reaction may be exacerbated, with significant systemic changes and potentially life-threatening. Even so, there is a lack of information about the management of these special cases, especially in oncopediatric cases. Therefore, the aim is to report a case of severe reaction post-accident with NaOCl in a 13-year-old adolescent undergoing chemotherapy, with significant edema, gingival necrosis, cutaneous rash, edema of the extremities, pleural effusion, and bronchopneumonia, with a 30-day evolution. It is understood that several factors, including anatomical, may predispose this situation, which can occur even with expert and experienced professionals, not necessarily associated with poor practice. The authors raise the importance of further studies to discuss the real need and indication of the use of NaOCl as an irrigating solution for these patients and suggest the adoption of extra safety measures to avoid the occurrence of similar situations.

Toxoplasma Appendicitis: A Once-in-a-Lifetime Diagnosis!

2017 ◽  
Vol 26 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Anisha M. Fernandes ◽  
Maheshkumar M. Lakhe ◽  
Sanjay A. Pai
Keyword(s):  
Differential Diagnosis ◽  
Acute Appendicitis ◽  
Muscularis Propria ◽  
Immunocompromised Patients ◽  
Lymphoid Follicles ◽  
Negative Results ◽  
Immunosuppressed Patients ◽  
Life Threatening

Toxoplasmosis is generally asymptomatic in immunocompetent individuals, but it can be life-threatening in immunocompromised patients. We present a case of a 62-year-old man with clinical features of acute appendicitis. Histology showed a transmural infiltrate of eosinophils. In addition, there were reactive lymphoid follicles with histiocytes in the submucosa and tachyzoites in the muscularis propria. Immunohistochemistry confirmed the diagnosis of toxoplasma appendicitis. Serological evaluation yielded negative results. Retrospective review of the history revealed that the patient was on long-term immunosuppressive therapy with methotrexate. The patient was treated with sulfamethoxazole-trimethoprim and is asymptomatic at 7-month follow-up. Toxoplasma appendicitis must be considered in the differential diagnosis of appendicitis in immunosuppressed patients.

scholarly journals Chromatin profiling reveals genome stability heterogeneity in clinical isolates of the human pathogen Aspergillus fumigatus

2021 ◽  
Author(s):  
Ana Cristina Colabardini ◽  
Fang Wang ◽  
Zhengqiang Miao ◽  
Lakhansing Pardeshi ◽  
Clara Valero ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Genome Stability ◽  
Genome Instability ◽  
Invasive Pulmonary Aspergillosis ◽  
Biosynthetic Gene Cluster ◽  
Clinical Isolates ◽  
Immunosuppressed Patients ◽  
Life Threatening ◽  
Metabolites Production ◽  
Chromatin Profiling

Invasive Pulmonary aspergillosis is a life-threatening infection in immunosuppressed patients caused by the filamentous fungus Aspergillus fumigatus. Chromatin structure regulation is important for genome stability maintenance and has the potential to lead to genome rearrangements driving differences in virulence and pathogenesis of different A. fumigatus isolates. Here, we compared the chromatin activities of the most investigated clinical isolates Af293 and CEA17 and uncovered striking differences in the number, locations and expression of transposable elements. We found evidence for higher genome instability in Af293 as compared to CEA17 and identified a spontaneous Af293 variant that exhibits gross chromosomal alterations including the loss of a 320 kb long segment in chromosome VIII and the amplification of a biosynthetic gene cluster. As a consequence of these re-arrangements, the variant shows increased secondary metabolites production, growth and virulence. Our work emphasizes genome stability heterogeneity as an evolutionary driver of A. fumigatus fitness and virulence.

scholarly journals Benefits of venom immunotherapy: How soon can they be expected

2017 ◽  
Vol 145 (3-4) ◽  
pp. 173-177
Author(s):  
Sasa Kadivec ◽  
Mitja Kosnik
Keyword(s):  
First Year ◽  
Severe Reaction ◽  
Allergic Reactions ◽  
Venom Immunotherapy ◽  
Emergency Situations ◽  
Sting Reaction ◽  
Life Threatening ◽  
Medical Emergencies ◽  
The Cost ◽  
Patients Experience

Introduction/Objective. Allergic reactions to insect stings are medical emergencies that could be prevented by venom immunotherapy (VIT). The main purpose of VIT is to prevent fatal or life-threatening reactions. We aimed to show the rapidity with which patients experience the benefits of VIT and estimate the number of emergency treatments that are prevented. Methods. We reviewed the medical files of patients who started VIT between 2010 and 2014. We calculated the costs of treatment of the sting reactions, the costs of immunotherapy, and estimated the costs of prevented allergic reactions. Results. In a cohort of 514 patients (40.9% female, age 47.2 ? 14.4 years), the cost of treatment of the index sting reaction was 180.4 ? 166.8 euros. During VIT, 195 patients experienced 446 field stings. In 86.3% of patients, stings were well tolerated, and only one patient experienced a severe reaction (grade III, according to Mueller). A total of 20.4% of VIT treated patients were stung during the first year of VIT and 57% during five years of VIT. The expenditure for five years of VIT was 2,886 euros per patient, which corresponded to an average of 16 emergency treatments for systemic reactions. Conclusion. Emergency situations are prevented in a substantial number of venom-allergic patients and a beneficial effect was already observed during the first year of VIT.

Emissions from Metallic Powder and High Energy-Based Process – Thermal Spraying, Powder Atomization, and Additive Manufacturing – Knowledge Refresh

2021 ◽  
Author(s):  
G. Darut ◽  
S. Dieu ◽  
B. Schnuriger ◽  
A. Vignes ◽  
O. Le Bihan ◽  
...  
Keyword(s):  
Ultrafine Particles ◽  
Scientific Literature ◽  
Health And Safety ◽  
Thermal Spraying ◽  
High Energy ◽  
Filtration Efficiency ◽  
Safety Measures ◽  
Worker Exposure ◽  
Lack Of Information ◽  
Manufacturing Knowledge

Abstract This paper addresses a need for information on nanoparticle emissions and related issues such as worker exposure, filtration efficiency, and dustiness. A survey has been conducted on the working conditions and safety measures used in thermal spray companies and the results compared to scientific literature and previous surveys. Responses to questions on matters of health and safety reveal a lack of information and awareness of the risks posed by the emissions of ultrafine particles generated by thermal spraying processes.

scholarly journals Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

2014 ◽  
Vol 25 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Marcos Rodolfo Bolfoni ◽  
Marcelo dos Santos Ferla ◽  
Otávio da Silva Sposito ◽  
Luciano Giardino ◽  
Rogério de Castilho Jacinto ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significance Level ◽  
Root Canals ◽  
Tukey Test ◽  
Irrigation Solution ◽  
Period Data

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

scholarly journals Antiadenovirus Activities of Several Classes of Nucleoside and Nucleotide Analogues

2005 ◽  
Vol 49 (3) ◽  
pp. 1010-1016 ◽  
Author(s):  
L. Naesens ◽  
L. Lenaerts ◽  
G. Andrei ◽  
R. Snoeck ◽  
D. Van Beers ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Colorimetric Assay ◽  
Human Embryonic Lung ◽  
Viral Dna ◽  
Nucleotide Analogues ◽  
Human Osteosarcoma Cells ◽  
Immunosuppressed Patients ◽  
Life Threatening ◽  
Infected Cells ◽  
Simplex Virus

ABSTRACT The absence of any formally licensed antiadenovirus drugs and the increasing incidence of life-threatening adenovirus infections in immunosuppressed patients warrant the development of effective antiadenovirus compounds. A detailed study was performed on the antiadenovirus activities of several classes of nucleoside and nucleotide analogues in human embryonic lung fibroblast cells. The antiadenovirus activities were evaluated by three methods, viz., evaluating the adenoviral cytopathic effect, monitoring cell viability by a colorimetric assay, and real-time PCR quantitation of viral DNA as a direct parameter for virus replication. The most active and selective compounds were the acyclic nucleoside phosphonate analogues cidofovir, its adenine analogue (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(S)-HPMPA], and the new derivative (S)-2,4-diamino-6-[3-hydroxy-2-(phosphonomethoxy)propoxy]pyrimidine [(S)-HPMPO-DAPy]; the N7-substituted acyclic derivative 2-amino-7-(1,3-dihydroxy-2-propoxymethyl)purine (S-2242); and the 2′,3′-dideoxynucleoside analogues zalcitabine and alovudine. No antiadenovirus activity was observed for the antiviral drugs ribavirin, foscarnet, acyclovir, penciclovir, and brivudin, while ganciclovir displayed modest activity. However, in human osteosarcoma cells transfected with herpes simplex virus thymidine kinase, ganciclovir demonstrated highly potent antiadenovirus activity, suggesting that the efficacy of ganciclovir against adenovirus is limited by inefficient phosphorylation in adenovirus-infected cells, rather than by insufficient inhibition at the viral DNA polymerase level. Collectively, our antiviral data show that the adenovirus DNA polymerase exhibits sensitivity to a relatively broad spectrum of inhibitors and should be studied further as an antiviral target in antiadenovirus drug development programs.

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Curcuma zedoaria dan Bahan Irigasi Natrium Hipoklorit 2.5% terhadap Enterococcus faecalis

2019 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
Martha Mozartha ◽  
Prisisilia Silvia ◽  
Billy Sujatmiko
Keyword(s):  
Antibacterial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Antibacterial Properties ◽  
Distilled Water ◽  
Turmeric Extract ◽  
Quasi Experimental ◽  
Irrigation Solution ◽  
Well Data ◽  
Weak Antibacterial Activity

Sodium hypochlorite (NaOCl) is a powerful disinfectant used in root canal therapy, yet it has some disadvantages. There are growing interests of using extracts of natural materials containing antibacterial secondary metabolite compounds as an alternative of irrigation solution. Aim, to compare the antibacterial activity of white turmeric extracts and 2,5% sodium hypochlorite irrigant against Enterococcus faecalis. Methods, this was a quasi experimental study. White turmeric extracts at the concentrations of 75%, 50%, 25% and  10% were used as experimental groups, while 2,5% NaOCl and distilled water were the control groups. Suspensions of Enterococcus faecalis were prepared to a 0.5 Mc Farland standard and applied onto petri plates containing Muller Hinton Agar. Six wells were created in each plate, and filled with 20 mL of the test solution. Plates were incubated at 37 ° C for 24 hours. The antibacterial activity was evaluated by measuring the clear zone around the well. Data were analyzed by Kruskall Wallis and continued by Mann U Whitney. Results, the largest growth inhibition zones were produced when the test bacteria were in contact with 2,5% NaOCl (15,0233 mm). White turmeric extract 75% concentration had weak antibacterial effect, while white turmeric extract 50%, 25%, 10% concentration, and distilled water did not has antibacterial properties against Enterococcus faecalis. Conclusion, white turmeric extract 75% concentration can inhibit the growth of Enterococcus faecalis with weak antibacterial activity.

Pepper-Spray-induced Respiratory Failure Treated With Extracorporeal Membrane Oxygenation

PEDIATRICS ◽  
1996 ◽  
Vol 98 (5) ◽  
pp. 961-963
Author(s):  
Deborah F. Billmire ◽  
Charles Vinocur ◽  
Maureen Ginda ◽  
Nancy B. Robinson ◽  
Howard Panitch ◽  
...  
Keyword(s):  
United States ◽  
Respiratory Failure ◽  
Extracorporeal Membrane Oxygenation ◽  
The United States ◽  
Accidental Exposure ◽  
Membrane Oxygenation ◽  
Self Defense ◽  
Lack Of Information ◽  
Life Threatening ◽  
Serious Injury

Self-defense sprays have been available to the general public since the 1970s. They are easily obtained and over 6 million spray units were sold in the United States in 1993 alone. The intent of these sprays is to cause skin and mucosal irritation without producing serious injury. We describe the accidental exposure to pepper-gas spray of an infant who experienced immediate life-threatening respiratory distress followed by progressive deterioration. Ultimately, he required extracorporeal membrane oxygenation (ECMO) for survival. The decision to place the infant on ECMO was made more difficult by the lack of information about potential reversibility with this rare toxic cause of respiratory failure.

Genotyping of Mycobacterium Tuberculosis Isolated from Suspected Patients in Tehran in 2015-2017

2019 ◽  
Author(s):  
Saeed Zaker ◽  
Hanie Bagherifard
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Lack Of Information ◽  
Iranian Studies ◽  
Pcr Products ◽  
Life Threatening ◽  
Epidemiological Pattern ◽  
Group 3 ◽  
Group 2

Background and Aims: Unlike many global efforts to eradicate tuberculosis caused by Mycobacterium, it remains as a life-threatening infection with a worldwide incidence of 1.5 million cases each year. However, due to the lack of information about Mycobacterium tuberculosis characterization, more studies are required to evaluate strain diversity and epidemiology of tuberculosis to improve the therapeutic approaches. This study aimed to genotype the Mycobacterium tuberculosis isolated from suspected patients in Tehran, Iran through 2015-2017.Materials and Methods: In the current study, 30 isolates (sputum, broncho-alveolar lavage and biopsy) were collected from different tuberculosis patients at Massoud Clinical Lab of Tehran from 2015 to 2017. To find the single nucleotide polymorphisms and mutated regions, polymerase chain reaction (PCR) was performed on all the isolates to amplify the katG and gyrA genes. Then, PCR products were sequenced and analyzed.Results: The majority of isolates were assigned to PGG2 (90%), followed by PGG3 (10%) but no isolate belonging to PGG1 was found.Conclusions: Our findings demonstrate a remarkable epidemiological pattern of tuberculosis in Tehran. In group 2, isolates showed a considerably higher frequency compared to isolates in group 3, which is consistent with other findings reported in Iran. However, in contrast to other Iranian studies, no isolated strains were categorized in principal PGG1.

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