Essential oils of Eplingiella fruticosa populations: chemical, antioxidant, and cytotoxic analyses

This study investigates the variations in the chemical profiles and biological activities (antioxidant and cytotoxic) of Eplingiella fruticosa from the state of Sergipe, an endemic species from the Northeast region of Brazil. The essential oils were extracted from six populations by hydrodistillation and analyzed by GC/MS-FID. Cluster analysis was performed with the data of the constituents of the essential oils, and then a dissimilarity matrix, based on Euclidean distances, and a dendrogram, through the Ward clustering method, were constructed. The antioxidant activity of the essential oils was tested by different assays (DPPH, ABTS, β-carotene, and FRAP), and cytotoxic activity was tested by the SRB assay. The compounds found in greater amounts were α-pinene, β-pinene, 1,8-cineole, camphor, borneol, δ-elemene, α-cubebene, α-ylangene, (E)-caryophyllene, germacrene D, bicyclogermacrene, trans-calamenene, spathulenol, caryophyllene oxide, and viridiflorol. These compounds defined the formation of two groups. The first group was composed of the populations of São Cristóvão, Itaporanga, Japaratuba, and Malhada dos Bois municipalities and was characterized by the presence of the monoterpene camphor (8.39-11.27%) as the compound of greatest concentration in relation to the other municipal areas. The second group was composed of the populations of Moita Bonita and Pirambu municipalities and was characterized by the major presence of the sesquiterpene bicyclogermacrene (7.45% and 10.98%). The plants exhibited weak effects in terms of antioxidant activity; however, the essential oil showed significant toxicity for the lines A549 (51.00% cell viability) in the population of Japaratuba, and B16F10 (64.94% cell viability) in Malhada dos Bois. The observations of this study may open a way to optimize the use of the E. fruticosa populations in relation to their cytotoxic properties.

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The Genus Haplophyllum Juss.: Phytochemistry and Bioactivities—A Review

Molecules ◽

10.3390/molecules26154664 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4664

Author(s):

Majid Mohammadhosseini ◽

Alessandro Venditti ◽

Claudio Frezza ◽

Mauro Serafini ◽

Armandodoriano Bianco ◽

...

Keyword(s):

Organic Acids ◽

Essential Oils ◽

Web Of Science ◽

Biological Activities ◽

Scientific Information ◽

Comprehensive Review ◽

Scientific Databases ◽

Organic Extracts ◽

Chemical Profiles ◽

Therapeutic Properties

Herein, a comprehensive review is given focusing on the chemical profiles of the essential oils (EOs), non-volatile compounds, ethnobotany, and biological activities of different Haplophyllum (Rutaceae family) species. To gather the relevant data, all the scientific databases, including Scopus, ISI-WOS (Institute of Scientific Information-Web of Science), and PubMed and highly esteemed publishers such as Elsevier, Springer, Taylor and Francis, etc., were systematically retrieved and reviewed. A wide array of valuable groups of natural compounds, e.g., terpenoids, coumarins, alkaloids, lignans, flavonoids, and organic acids have been isolated and subsequently characterized in different organic extracts of a number of Haplophyllum species. In addition, some remarkable antimicrobial, antifungal, anti-inflammatory, anticancer, cytotoxic, antileishmanial, and antialgal effects as well as promising remedial therapeutic properties have been well-documented for some species of the genus Haplophyllum.

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Natural terpenes as substances with multidirectional biological activities

Postępy Polskiej Medycyny i Farmacji ◽

10.5604/01.3001.0013.2320 ◽

2019 ◽

Vol 6 ◽

pp. 43-50

Author(s):

Karolina A. Wojtunik-Kulesza ◽

Monika Waksmundzka- Hajnos

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Essential Oils ◽

Biological Activities ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Lemon Balm ◽

The World ◽

Alzhiemer's Disease ◽

Main Components

Terpenes, secondary metabolites, are considered by scientists around the World. Multidirectional biological activities of these substances encourage researchers to their detail analysis towards numerous diseases such as neurodegenerative or metabolic. In the presented paper, authors have focused on Alzheimer’s disease and diabetes as potential direction of terpenes’ activity. According to available literature, terpenes exhibit satisfactory antioxidant activity and ability to inhibition AChE, BuChE (Alzhiemer’s disease) and α-amylase, α-glucosidase (diabetes). Basis of the presented research are both essential oils obtained from plants such as salvia, rosemary, basil and lemon balm being one of the main source of natural terpenes, and single compounds. Special attention is paid to monoterpenes as the main components of numerous essential oils. Among terpenes presented in the paper, activity of ocimene, carvone, pulegone, α-phellandrene exhibiting activity towards both free radical scavenging as well as AChE and BuChE inhibition, and α-pinene, citral, limonene that revealing inhibitory activity towards α-amylase and α-glucosidase should be emphasized.

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Habitat Affects the Chemical Profile, Allelopathy, and Antioxidant Properties of Essential Oils and Phenolic Enriched Extracts of the Invasive Plant Heliotropium Curassavicum

Plants ◽

10.3390/plants8110482 ◽

2019 ◽

Vol 8 (11) ◽

pp. 482 ◽

Cited By ~ 8

Author(s):

Ahmed M. Abd-ElGawad ◽

Abdelsamed I. Elshamy ◽

Saud L. Al-Rowaily ◽

Yasser A. El-Amier

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Essential Oils ◽

Invasive Plant ◽

Biological Activities ◽

Antioxidant Properties ◽

Bioactive Constituents ◽

Chenopodium Murale ◽

Two Samples ◽

Ic50 Values

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.

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CHEMICAL PROFILING OF BUCHANANIA LANZAN SPRENG ESSENTIAL OIL AND ITS BIOLOGICAL ACTIVITIES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2020.v13i12.39573 ◽

2020 ◽

pp. 103-108

Author(s):

AJITH S ◽

KRISHNA V ◽

RAVI KUMAR S ◽

VINAY KUMAR NM

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Molecular Docking ◽

Essential Oil ◽

Essential Oils ◽

Bioactive Compounds ◽

Biological Activities ◽

Dna Gyrase ◽

Diffusion Method

Objective: The present study was designed to evaluate the chemical composition of the essential oil of Buchanania lanzan Spreng extracted from the seeds and to evaluate in vitro antimicrobial antioxidants and molecular docking studies of the major bioactive compounds of essential oil. Methods: The essential oil was obtained by hydrodistillation of the B. lanzan seeds and analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was evaluated against Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, Staphylococcus aureus, and Streptococcus pneumoniae clinical isolates by disk diffusion method and resazurin assay determined the minimum inhibitory concentration. The in vitro antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging assay; the essential oil major bioactive compounds are Androstan-3-ol, Campesterol, and γ-Sitosterol were docked against bacterial protein DNA gyrase. Results: GC-MS analysis exhibited the presence of 19 bioactive compounds. The essential oil showed that significant antibacterial activity was noticed against V. cholerae and S. typhi with the highest zone of inhibition 15.67–1.20 and 13.83–0.33, respectively. Antioxidant activity in DPPH and H2O2 scavenging assays with IC50 values of 134.23 and 191.24, respectively. The molecular docking of Androstan-3-ol and γ-Sitosterol with bacterial DNA gyrase unveiled a good binding affinity of −6.4 kcal/mol and −6.3 kcal/ mol, respectively. Conclusion: It could be concluded that the essential oils potential sources of antibacterial, antioxidant activities, and molecular docking of bioactive components. The results of this study provide partial scientific support for the traditional application of essential oils to cure diarrhea and also major bioactive compounds responsible for important biological activities.

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Chemical Compositions and Biological Activities of Essential Oils of Beilschmiedia glabra

Natural Product Communications ◽

10.1177/1934578x1501000740 ◽

2015 ◽

Vol 10 (7) ◽

pp. 1934578X1501000 ◽

Cited By ~ 4

Author(s):

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Farediah Ahmad ◽

Khong Heng Yen ◽

Razauden Mohamed Zulkifli

Keyword(s):

Essential Oils ◽

Phenolic Content ◽

Biological Activities ◽

Chemical Compositions ◽

Strong Activity ◽

Germacrene D ◽

Leaf Oil ◽

Weak Activity ◽

Anti Inflammatory ◽

Β Carotene

This study was designed to examine the chemical compositions of essential oils from Beilschmiedia glabra and their antioxidant, antimicrobial, antityrosinase, acetylcholinesterase and anti-inflammatory activities. In total, 47 components were identified in the essential oils, which made up 86.8% and 89.7% of the leaf and bark oils, respectively. The leaf oil is composed mainly of β-eudesmol (15.4%), β-selinene (12.2%), caryophyllene oxide (8.1%) and γ-gurjunene (5.2%), while the bark oil contains high percentages of β-eudesmol (19.3%), β-selinene (16.9%), δ-cadinene (15.8%), germacrene D (9.8%) and β-caryophyllene (5.5%). Antioxidant activity showed that the leaf oil has the highest phenolic content at 233.4 mg GA/g, while the bark oil showed potent activity in the β-carotene/linoleic acid bleaching assay. However, both oils showed weak activity in the DPPH and ABTS assays. For antimicrobial activity, the leaf and bark oils displayed strong activity against Candida glabrata and Saccharomyces cerevisiae with MIC values of 31.3 and 62.5 μg/mL, respectively. Percentage inhibitions against tyrosinase (leaf 73.7%; bark 76.0%) and acetylcholinesterase (leaf 48.1%; bark 45.2%) were tested at a concentration of 1 mg/mL, while anti-inflammatory activity (leaf 59.7%; bark 48.9%) was evaluated at a concentration of 100 μM. Evaluation of these assays indicated moderate levels of activity.

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Antioxidant and Antibacterial Activity of Roseroot (Rhodiola rosea L.) Dry Extracts

Molecules ◽

10.3390/molecules23071767 ◽

2018 ◽

Vol 23 (7) ◽

pp. 1767 ◽

Cited By ~ 10

Author(s):

Olga Kosakowska ◽

Katarzyna Bączek ◽

Jarosław L. Przybył ◽

Ewelina Pióro-Jabrucka ◽

Weronika Czupa ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Phenolic Compounds ◽

Biological Activities ◽

Rhodiola Rosea ◽

Array Detector ◽

Chemical Compositions ◽

Antioxidant Power ◽

Scavenging Capacity ◽

Chemical Profiles

Roseroot (Rhodiola rosea L.) belongs to plants revealing adaptogenic properties, which are attributed to the presence of specific phenolic compounds and are reflected mainly as antioxidant activity. The aim of the present study was to determine the antioxidant and antibacterial activity of various products obtained from R. rosea (underground organs as well as their aqueous and ethanolic dry extracts) in relation to the chemical profiles of phenolic and essential oil compounds. The chemical profiles were determined by High-performance Liquid Chromatography with a diode-array detector (HPLC-DAD) and Gas chromatography-mass spectrometry (GC-MS), antioxidant activity by (1,1-Diphenyl-2-picrylhydrazyl) Scavenging Capacity Assay (DPPH), (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) Scavenging Capacity Assay (ABTS) and Ferric Reducing Antioxidant Power Assay (FRAP) and antimicrobial properties were expressed as minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) values following the broth microdilutions method. The results show that the investigated samples differed in terms of their chemical compositions and biological activities. The extracts were more abundant in phenolic compounds (salidroside, tyrosol, and rosavin derivatives) in comparison to dried underground organs. The content of the determined phenolics in the analyzed extracts was affected by the solvent used for extraction. The ethanolic extract was characterized by the highest content of these substances in comparison to the aqueous one and the dried raw material, especially with regard to rosavin (969.71 mg/100 g). In parallel, this extract showed the strongest antioxidant and antibacterial activity. However, dried R. rosea underground organs also revealed strong antibacterial effects against, for example, Staphylococcus strains.

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Chemical characterization of essential oils and antioxidant activity of Dictyota dichotoma and Dictyopteris membranacea

Acta periodica technologica ◽

10.2298/apt1950033b ◽

2019 ◽

pp. 33-42

Author(s):

Redhouane Benfares ◽

Affaf Kord ◽

Khaled Boudjema ◽

Manel Bouarab ◽

Soumia Benrabah ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oils ◽

Steam Distillation ◽

Chemical Characterization ◽

Dictyota Dichotoma ◽

Chemical Screening ◽

Similar Chemical ◽

Ic50 Values ◽

Chemical Profiles ◽

Main Component

The work focuses on the study of two brown algae Dictyota dichotoma and Dictyopteris membranacea. Extracts obtained by different organic solvents (dichloromethane, hexane, acetone, methanol, chloroform) were screened for their DPPH antiradical activity, whereas essential oils obtained by steam distillation were chemically characterized by GC-MS. Steam distillation yielded 0.07 and 0.095% of the essential oils for Dictyota dichotoma and Dictyopteris membranacea, respectively. The chemical screening of the essential oils of Dictyopteris membranacea and Dictyota dichotoma showed similar chemical profiles. Namely, trans-anethole showed to be the main component in both studied oils, yielding 12.28% and 10.70% for Dictyopteris membranacea and Dictyota dichotoma, respectively. Antioxidant activity of extracts was expressed by IC50 values and was in the range 0.375-1.72 mg/ml.

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Essential Oil Composition, Antioxidant, Anticholinesterase and Anti-tyrosinase Activities of Two Turkish Plant Species: Ferula elaeochytris and Sideritis stricta

Natural Product Communications ◽

10.1177/1934578x1801300130 ◽

2018 ◽

Vol 13 (1) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Ebru Deveci ◽

Gülsen Tel-Çayan ◽

Mehmet Emin Duru

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Radical Scavenging ◽

Cation Radical ◽

Essential Oil Composition ◽

Chemical Compositions ◽

Oil Composition ◽

Metal Chelating ◽

Β Carotene

The aim of the present study is to characterize chemical compositions and antioxidant, anticholinesterase and anti-tyrosinase activities of Ferula elaeochytris and Sideritis stricta essential oils. The hydrodistilled essential oils were analyzed by GC/FID and GC/MS. A total of thirty-three and twenty-seven compounds representing 99.6 % and 99.4 % were identified in F. elaeochytris and S. stricta, respectively. The main compounds of essential oil of F. elaeochytris were β-cubebene (21.3 %), caryophyllene oxide (17.5 %) and β-caryophyllene (14.9 %), while the major compounds of S. stricta essential oil were δ-cadinene (18.3 %), cubenol (17.6 %) and β-caryophyllene (14.4 %). The antioxidant activity was tested by β-carotene-linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC and metal chelating assays. The essential oil of F. elaeochytris showed the highest antioxidant activity in all assays. Also, the anticholinesterase and anti-tyrosinase activities of essential oils were performed against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase enzymes. F. elaeochytris essential oil indicated the highest anticholinesterase and anti-tyrosinase activities as well. This is the first report describing antioxidant, anticholinesterase and anti-tyrosinase activities of essential oils of F. elaeochytris and S. stricta.

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Chemical Composition and Biological Activities of Essential Oils of Echinops spinosus and Carlina vulgaris Rich in Polyacetylene Compounds

Current Nutrition & Food Science ◽

10.2174/1573401315666190206142929 ◽

2020 ◽

Vol 16 (4) ◽

pp. 563-570

Author(s):

Rania Belabbes ◽

Imane R. Mami ◽

Mohammed E.A. Dib ◽

Kenza Mejdoub ◽

Boufeldja Tabti ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Fruits And Vegetables ◽

Insect Pests ◽

Biological Activities ◽

Bactrocera Oleae ◽

Chemical Compositions ◽

Antioxidant Power

Background: The essential oils of aromatic plants are increasingly used as new biocontrol alternative agents against microbial strains and insect pests of fruits and vegetables, because of their specificity of biodegradable nature. Objective: This work, treats for the first time the chemical composition, antioxidant, antifungal and insecticidal activities of the essential oils obtained from Echinops spinosus and Carlina vulgaris from Algeria. Methods: The chemical compositions of oils were investigated using GC-FID and GC/MS. Antioxidant activity was assessed using three methods (2,2-diphenyl-1-picrylhydrazyl, Ferric-Reducing Antioxidant Power (FRAP) and β-carotene assay). Fumigation toxicity of E. spinosus and C. vulgaris essential oils was tested against Bactrocera oleae pests of olives. Results: Eighteen and thirteen components representing 95.4 and 97.9% were identified in root essential oils from Echinops spinosus and Carlina vulgaris, respectively. Polyacetylenes were the majority compounds of essential oils. 5 (But-1-yn-3-enyl).2,2'bithiophene and α-terthienyle were highly dominants in the E. spinosus essential oil from the roots (54.4 and 26.3%, respectively). Roots of C. vulgaris produce an essential oil dominated by carlina oxide (33.7%) and 13-methoxy carlina oxide (11.5%). Comparison of the antioxidant activity of E. spinosus essential oil showed more important antioxidant effect than C. vulgaris essential oil and the synthetic antioxidant (BHT). Evaluation of the antifungal activity showed an interesting efficiency of both essential oils against P. expansum and A. niger with EC50s varied from 5 to 14.5 mg/L. C. arvensis essential oil exhibited good larvicidal properties. At the concentration of 325 μL/L air, the oil caused mortality of 100% for Bactrocera Oleae adults after 24 h of exposure. Conclusion: Both essential oils rich to polyacetylenes and polythiophenes.components have shown interesting biological activities, which suggests that plants have the potential to be used as biopesticides and provide an alternative to chemical pesticides.

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Chemometric Comparison and Classification of Some Essential Oils Extracted from Plants Belonging to Apiaceae and Lamiaceae Families Based on Their Chemical Composition and Biological Activities

Molecules ◽

10.3390/molecules23092261 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2261 ◽

Cited By ~ 9

Author(s):

Cristina Semeniuc ◽

Maria-Ioana Socaciu ◽

Sonia Socaci ◽

Vlad Mureșan ◽

Melinda Fogarasi ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Biological Activities ◽

Principal Component ◽

Total Phenolic ◽

Chemometric Methods ◽

Weak Antibacterial Activity

This study is focused on the comparison and classification of parsley, lovage, basil, and thyme essential oils (EOs) based on their chemical composition, total phenolic content, antioxidant and antibacterial activities by using appropriate chemometric methods: Principal component analysis (PCA) and hierarchical cluster analysis (HCA). The results showed that parsley, lovage, and thyme EOs are rich in monoterpene hydrocarbons, but basil EO is rich in oxygenated monoterpenes and phenylpropanoids, and that both PCA and HCA separated essential oils into two main groups of which one contains two sub-groups. β-Phellandrene was the major component identified in parsley and lovage EOs, estragole was the major component in basil EO, and p-cymene was the major component in thyme EO. Thyme EO showed the highest level of total phenolics, the highest antioxidant capacity, and exhibited the stronger antibacterial activity, results that were emphasized by both chemometric methods used. Among tested essential oils, the one of parsley was distinguished by a low total TPC, weak antioxidant activity, and weak antibacterial activity against S. enteritidis (ATCC 13076); lovage EO by low TPC, weak antioxidant activity, but moderate antibacterial activity; and basil EO by low TPC, moderate antioxidant activity, and weak antibacterial activity against L. monocytogenes (ATCC 19114).

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