scholarly journals Habitat Affects the Chemical Profile, Allelopathy, and Antioxidant Properties of Essential Oils and Phenolic Enriched Extracts of the Invasive Plant Heliotropium Curassavicum

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Invasive Plant ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Bioactive Constituents ◽  
Chenopodium Murale ◽  
Two Samples ◽  
Ic50 Values

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.

scholarly journals Etude De L’Activité Antioxydante Des Huiles Essentielles De Plantes Aromatiques Et Médicinales Marocaines

2017 ◽  
Vol 13 (12) ◽  
pp. 323
Author(s):  
Rachid Ismaili ◽  
Sara Houbairi ◽  
Lanouari Sanâa ◽  
Moustaid Khadija ◽  
Lamiri Abdeslam
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Wild Plants ◽  
Thymus Vulgaris ◽  
Fruit Peel ◽  
Mentha Spicata ◽  
Strong Activity ◽  
Citrus Limonum

Morocco is a traditional supplier of medicinal and aromatic plants to the world. This activity has resulted to the exploitation of wild plants than dried plants for herbal needs and food flavors. Many species are used for the production of plant extracts and other aromatic essences mainly for the pharmaceutical and cosmetics industry. Among these metabolites, there are essential oils that are mixtures of aromatic substances present in the form of tiny droplets in the leaves, fruit peel, resin, branches, and wood. These oils, however, is endowed with antioxidant properties. This study aims to evaluate the antioxidant activity of essential oils of three moroccan aromatic and medicinal plants: Thymus vulgaris, Mentha spicata, and Citrus limonum. The oil extraction was performed by steam distillation in a Clevenger type apparatus. Also, the antioxidant activity of these oils was evaluated by the method of DPPH (2,2-diphenyl-1-picrylhydrazyl), in comparison with the antioxidant synthetic, ascorbic acid. In the present work, the results showed that the antioxidant activity of three essential oils studied by the trapping method of free radical is moderate. Also, the oil of Thymus vulgaris has a strong activity which exceeds that of ascorbic acid. This was followed by the oil of Mentha spicata, while the lowest activity was observed in Citrus limonum.

scholarly journals In Vitro Effect of Plant Parts Extract of Senecio glaucus L. on Pathogenic Bacteria

2021 ◽  
Vol 12 (3) ◽  
pp. 3800-3810
Keyword(s):  
Chemical Composition ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Bioactive Constituents ◽  
Two Samples ◽  
Ic50 Values ◽  
Vitro Effect

Senecio glaucus L. is an annual herb that grows in several Egyptian desert habitats. The diversity of habitats inhabited by this species, as well as its distribution, chemical composition, and biological activity, are all unknown. This research aimed to examine the chemical composition of S. glaucus from various environments in Egypt, as well as the antioxidant and antimicrobial activities. The general assessment of the analytical results for different parts of S. glaucus showed that the capitula and leaves in both inland and coastal samples were rich in bioactive constituents than the other parts as following (capitula > leaf > root > stem). Based on the results of IC50, the antioxidant properties of the eight parts of two samples follows the sequence capitula ˃ root ˃ leaf ˃ stem for the coastal sample, and capitula ˃ leaf ˃ stem ˃ root for the inland sample. The IC50 values ranged from 25.94 to 41.20 mg/ml in coastal sample, where the IC50 values ranged from 28.02 to 42.83 mg/ml in desert sample, compared to ascorbic acid (IC50 = 13.30 mg/ml). The antimicrobial potential of MeOH extracts of S. glaucus parts collected from different habitats exhibited different inhibitory spectrum behavior with varying degrees of inhibition against six Gram-positive bacteria and four Gram-negative bacteria. In both coastal and inland samples, the E. coli inhibition zone was the most susceptible bacterium. Whereas, in the case of the coastal sample, the inhibition zone of B. subtilis was the most sensitive bacterium. The results of the antibacterial test were compared with 3 standard antibiotics.

scholarly journals Chemical composition and biological activities of essential oils of endemic Thymus leucostomus Hausskn. et Velen

2020 ◽  
Vol 49 (4) ◽  
pp. 957-965
Author(s):  
Omer Elkiran ◽  
Cumhur Avsar
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gram Positive Bacteria ◽  
In Vitro Antioxidant Activity

The chemical composition, antimicrobial and antioxidant properties of the essential oils from the leaves of endemic Thymus leucostomus naturally grown in Turkey were investigated and chemical differences were discussed by means of chemotaxonomy. Twenty-six components were identified representing 98.8% of the oils. The main compounds in the essential oil of T. leucostomus were: o-cymene (30.6%), carvacrol (9.6%), thymol methyl ether (7.2%), limonene (6.8%). Essential oil was screened for their antimicrobial activities against 7 bacteria and 2 yeast species by using disc-diffusion and MIC procedure. The essential oil showed higher effectiveness against all the tested bacteria and yeast. The extract was observed to be much more effective in Gram-positive bacteria (especially, S. aureus ATCC 6538). In vitro antioxidant activity based on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical was evaluated for the essential oil, and it was found that the essential oil had good antioxidant activity in the range of the IC50= 5.42 ±0.8 μg/ml.

scholarly journals Essential oil composition and antioxidant activity of two Juniperus communis L. varieties growing wild in Serbia

2016 ◽  
pp. 197-205 ◽  
Author(s):  
Nemanja Rajcevic ◽  
Tanja Dodos ◽  
Jelica Novakovic ◽  
Pedja Janackovic ◽  
Petar Marin
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oil ◽  
Essential Oils ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Dpph Assay ◽  
Juniperus Communis ◽  
Ic50 Values ◽  
Typical Variety

The genus Juniperus L. (Cupressaceae) consists of ca. 67 species and 34 varieties. Juniperus communis L. grows on dry hills or mountainous tracts and is widely distributed in the northern hemisphere. A typical variety J. communis L. var. communis was collected in Deliblatska pescara (Deliblato Sands) and variety J. communis L. var. saxatilis Pall. in Kopaonik Mountain. Needle essential oils were obtained using Clevenger apparatus and analyzed using GC/MS and GC/FID. Antioxidant activity of essential oils was evaluated using DPPH assay. A total of 78 compounds were detected and identified. Both oils are characterized by high abundance of monoterpenes. The main constituents of J. communis var. communis essential oil were sabinene (39.4%), ?-pinene (13.3%), myrcene (4.7%) and terpinen-4-ol (3.7%), while J. communis var. saxatilis essential oil had ?-pinene (34.9%), sabinene (20.3%), ?-3-carene (6.4%) and germacrene B (6.3%) as the most abundant components. DPPH test showed IC50 values 0.66 mg/ml for J. communis var. communis and 0.32 mg/ml for J. communis var. saxatilis. Although antioxidant activity was weaker than used standards (BHT and L-ascorbic acid) it is still significant.

scholarly journals The antioxidant properties of dried extracts from the spent espresso coffee

2013 ◽  
Vol 67 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Milica Milutinovic ◽  
Slavica Siler-Marinkovic ◽  
Dusan Antonovic ◽  
Katarina Mihajlovska ◽  
Marija Pavlovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Butylated Hydroxytoluene ◽  
Prooxidant Activity ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Espresso Coffee

The importance of coffee waste utilization is based on the fact that it contains a large amount of biologically valuable components. Preparation of espresso coffee produces substantial quantities of polyphenolic acids that have a significant antioxidant activity. In this work, the contents of polyphenols and antioxidant activity of extracts obtained from spent espresso coffee were analyzed using different solvent systems. The 70% solution of methanol, and 70% solution of ethanol and distilled water were used as solvents. The total amounts of polyphenols were determined by the Folin-Ciocalteu method, while the antioxidant activities were determined by DPPH inhibition (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power). Both methods confirmed that the order of antioxidant activity of obtained dried extracts is as follows: water extract < methanol extract < ethanol extract, which was in accordance with their polyphenols content. Mild prooxidant activity was observed in the concentration of 200 ?g/ml while investigating the DPPH inhibition. Prooxidant activity is a characteristic of some polypenolic acids that is considered to be one of the mechanisms of anticancer activity. The inhibition of DPPH radical, expressed by IC50 values, was compared with the results of the commercial antioxidants such are ascorbic acid and BHT (butylated hydroxytoluene). Comparing the IC50 values of ascorbic acid and BHT with the IC50 values of tested extracts it can be concluded that waste coffee extracts could be a good source of natural antioxidants.

scholarly journals Quantification of Major Bioactive Constituents, Antioxidant Activity, and Enzyme Inhibitory Effects of Whole Coffee Cherries (Coffea arabica) and Their Extracts

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4306
Author(s):  
Boris Nemzer ◽  
Diganta Kalita ◽  
Nebiyu Abshiru
Keyword(s):  
Antioxidant Activity ◽  
Coffea Arabica ◽  
Digestive Enzymes ◽  
Chlorogenic Acids ◽  
Inhibitory Effects ◽  
Bioactive Constituents ◽  
Caffeoylquinic Acids ◽  
Dicaffeoylquinic Acid ◽  
Ic50 Values ◽  
Control Of Diabetes

Coffee cherry is a rich source of chlorogenic acids (CGAs) and caffeine. In this study we examined the potential antioxidant activity and enzyme inhibitory effects of whole coffee cherries (WCC) and their two extracts on α-amylase, α-glucosidase and acetylcholinesterase (AChE) activities, which are targets for the control of diabetes and Alzheimer’s diseases. Whole coffee cherry extract 40% (WCCE1) is rich in chlorogenic acid compounds, consisting of a minimum of 40% major isomers, namely 3-caffeoylquinic acids, 4-caffeoylquinic acids, 5-caffeoylquinic acids, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 4-feruloylquinc acid, and 5-feruloylquinc acid. Whole coffee cherry extract 70% (WCCE2) is rich in caffeine, with a minimum of 70%. WCCE1 inhibited the activities of digestive enzymes α-amylase and α-glucosidase, and WCCE2 inhibited acetylcholinesterase activities with their IC50 values of 1.74, 2.42, and 0.09 mg/mL, respectively. Multiple antioxidant assays—including DPPH, ABTS, FRAP, ORAC, HORAC, NORAC, and SORAC—demonstrated that WCCE1 has strong antioxidant activity.

VARIETAL FEATURES OF TUBERS OF JERUSALEM ARTICHOKE, GROWING IN THE KRASNODAR REGION AND THE Adygea Republic

2019 ◽  
Author(s):  
Н.Т. Шамкова ◽  
В.Ю. Токарев ◽  
А.В. Добровольская ◽  
А.З. Тодорова ◽  
А.И. ВЕРЕЩАГИНА
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Orotic Acid ◽  
Antioxidant Properties ◽  
Jerusalem Artichoke ◽  
Biotechnological Potential ◽  
Total Sugars ◽  
Krasnodar Region ◽  
Public Catering ◽  
Pectin Substances

Определен биотехнологический потенциал топинамбура сортов Новость ВИР, Скороспелка, Интерес, произрастающего в Краснодарском крае и Республике Адыгея, урожаев 2011 2018 гг. Установлено, что клубни топинамбура сорта Интерес превосходят сорта Новость ВИР и Скороспелка по содержанию, : общих сахаров на 22,41 и 14,34 инулина 41,82 и 32,99 пектиновых веществ 22,39 и 49,75 аскорбиновой кислоты 35,27 и 39,29 хлорогеновой кислоты 3,66 и 18,29 оротовой кислоты 88,89 и 84,89 соответственно. Сорт Интерес в сравнении с сортами Новость ВИР и Скороспелка содержит на 245,24 и 243,33 меньше никотиновой кислоты и на 100,0 и 108,33 кофейной кислоты соответственно. Содержание гистидина в клубнях топинамбура сортов Интерес, Новость ВИР и Скороспелка составляет 132,36 44,83 и 88,21 мг/кг аргинина 2017,2 958,56 и 460,44 мг/кг соответственно. В комплексе с аскорбиновой кислотой органические кислоты клубней топинамбура обладают выраженными антиоксидантными свойствами. Установлено, что в результате бланширования антиоксидантная активность топинамбура сорта Интерес возросла на 15,6. Таким образом, сорт Интерес наиболее перспективен для переработки в условиях предприятий общественного питания. The biotechnological potential of Jerusalem artichoke varieties Novost VIR, Skorospelka, Interes growing in the Krasnodar region and the Adygea Republic, harvest 2011 2018 has been determined. It was found that Jerusalem artichoke tubers of the Interes variety are superior to Novost VIR and Skorospelka varieties in content, : total sugars by 22,41 and 14,34 inulin 41,82 and 32,99 pectin substances 22,39 and 49,75 ascorbic acid 35,27 and 39,29 chlorogenic acid 3,66 and 18,29 orotic acid 88,89 and 84,89, respectively. Variety Interes in comparison with Novost VIR and Skorospelka varieties contains 245,24 and 243,33 less nicotinic acid and 100,00 and 108,33 coffee acid, respectively. The content of histidine in the tubers of Jerusalem artichoke varieties of Interes, Novost VIR, Skorospelka is 132,36 44,83 and 88,21 mg/kg arginine 2017,2 958,56 and 460,44 mg/kg, respectively. In a complex with ascorbic acid, organic acids of Jerusalem artichoke tubers have pronounced antioxidant properties. It was found that as a result of blanching, the antioxidant activity of Jerusalem artichoke of the Interes variety increases by 15,6. Thus, the Interes variety is most promising for processing in public catering facilities.

scholarly journals Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract

2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Antioxidant Properties ◽  
Linear Connection ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Control Group

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.

scholarly journals Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

2020 ◽  
pp. 1-5
Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
Apium Graveolens ◽  
Isolation And Identification ◽  
Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

Novel 1,2-thiazine-pyridine hybrid: Design, synthesis, antioxidant activity and molecular docking study

2022 ◽  
Vol 19 ◽  
Author(s):  
Rania B. Bakr ◽  
Nadia A.A. Elkanzi
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Docking Study ◽  
Molecular Docking Study ◽  
Sod Activity ◽  
Design Synthesis ◽  
In Silico Docking

Background & objectives: 1,2-thiazine and pyridine heterocycles drew much attention due to their biological activities including antioxidant activity. Based upon fragment based drug design, novel pyrido[1,2]thiazines 9a-c, thiazolidinopyrido[1,2]thiazines 10a-c and azetidinopyrido[1,2]thiazines 11a-c were designed and prepared. Methods: These novel derivatives 9a-c, 10a-c and 11a-c were subjected to screening for their antioxidant activity via various assays as DPPH radical scavenging potential, reducing power assay and metal chelating potential. Results: All the assayed derivatives exhibited excellent antioxidant potential and the tested compounds 9a, 9b, 10a, 10b, 11a and 11b exhibited higher DPPH scavenging potential (EC50 = 32.7, 53, 36.1, 60, 40.6 and 67 µM, respectively) than ascorbic acid (EC50 = 86.58 µM). While targets 9a, 10a and 11a (RP50 = 52.19, 59.16 and 52.25 µM, respectively) exhibited better reducing power than the ascorbic acid (RP50 = 84.66 µM). Computational analysis had been utilized to prophesy the bioactivity and molecular properties of the target compounds. Conclusion: To predict the binding manner of the novel derivatives as antioxidants, in-silico docking study had been performed to all the newly prepared compounds inside superoxide dismutase (SOD) and catalase (CAT) active site. The most active antioxidant candidate 9a (EC50 = 32.7 µM, RP50 = 52.19 µM) displayed excellent binding with Lys134 amino acid residing at Cu-Zn loop of SOD with binding energy score = -7.54 Kcal/mol thereby increase SOD activity and decrease reactive oxygen species.

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