Cytotoxicity in fibroblasts from young and elderly donors from two mouthwashes used to prevent the spread of SARS-CoV-2

In cases of injuries in the oral cavity, the mouthwash comes in contact with the underlying gingival connective tissue and should have its cytotoxicity assessed. However, there is no available evidence if cells of elderly donors react differently during in vitro assessments of mouthwashes. This study aimed to compare the cytotoxicity evaluation of two different mouthwash types when assessed with primary gingival fibroblasts from either young and older donors. Primary cells were collected from two elderly patients (mean age 66.5 years old) and two young patients (mean age 27.5 years old). The primary cell culture was produced from gingival fragments and exposed for 24h in Perioxidin® and Oral B®. A control group was exposed to unconditioned culture media, representing 100% of cell survival (negative control), and 200mg/mL solution of latex fragments was used as a positive control due to its well-known toxicity. Both products presented similar dose-dependent cytotoxicity. In the toxic range, from 0.035% to 0.00035% for Perioxidin® and 0.06 to 0.0006% for Oral B®. The calculated IC50 values were very similar, with the exception of Oral B® tested with young cells, which presented a slightly higher toxic concentration (0.0523 mM). The statistical analysis shows no significant difference between tests with cells from young our elderly donors (p >0.05). These mouthwashes should should be used sparingly to prevent the spread of SARS-CoV-2. However, the use of age-matched cells during in vitro tests may not be necessary to predict differences in the biological response of the elderly to these products.

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Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v3 ◽

2020 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs. Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm. Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively. Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

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Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f090201 ◽

2011 ◽

Vol 9 (2) ◽

pp. 33-37

Author(s):

MUHAMMAD ARIF NUR SYAHID ◽

CR. SITI UTARI ◽

SUTARMIADJI DJUMARGA

Keyword(s):

Ascaris Suum ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Mimosa Pudica ◽

Control Group Design ◽

Significance Level ◽

Death Time ◽

Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

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Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v2 ◽

2020 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar ◽

...

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs.Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm.esults The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively.Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

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In Vitro Comparison of MTA and BC RRM-Fast Set Putty as Retrograde Filling Materials

10.21203/rs.3.rs-1099531/v1 ◽

2021 ◽

Author(s):

Carla Arvelaiz ◽

Andreina Fernandes ◽

Veronica Graterol ◽

Katiuska Gomez ◽

Jose Francisco Gomez-Sosa ◽

...

Keyword(s):

Brain Heart Infusion ◽

Bacterial Count ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Material ◽

Human Teeth ◽

Retrograde Filling ◽

Significant Difference

Abstract Background: To compare, in vitro, the bioceramic materials (MTA and BC RRM-fast set putty) capacity to prevent microleakage of Enterococcus faecalis over time. Methods: An experimental design was made with forty extracted human teeth, coronally cut, and prepared to be placed in a leakage system under sterile conditions. They were randomly divided into an experimental group: thirty teeth (fifteen for retrograde filling material MTA and BC RRM-fast set putty, respectively) and a control group: ten teeth (five positive control, five negative control). The 3 mm root-ends were submerged in a brain-heart infusion broth with a red phenol indicator. The coronal access of each sample was inoculated with E. faecalis every seven days to maintain bacterial viability. The lower chamber was evaluated daily for 30 days to observe the turbidity of the culture medium and establish the presence and day of the filtration. Calculation of the colony-forming units (CFU) was performed for each leaked sample. Results: Of the total samples prepared for each type of bioceramic material, leaked 60.0% (9/15) of the MTA group and 40.0% (6/15) of the BC RRM-fast set putty group. All positive controls filtered on the first day of evaluation, while 20% (1/5) of the negative control leaked in the second week. There was no significant difference in leakage between the bioceramic material types, nor concerning the bacterial count and the type of cement used (p = 0.101). Conclusions: This study suggests that BC RRM-fast set putty and MTA have a similar sealing capacity when used as a retrograde filling material in vitro.

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Spectrophotometric Analysis Evaluating Apical Microleakage in Retrograde Filling using GIC, MTA and Biodentine: An in-vitro study

10.21203/rs.2.15368/v1 ◽

2019 ◽

Author(s):

Manisha Nepal ◽

Snigdha Shubham ◽

Rupam Tripathi ◽

Jwolan Khadka ◽

Deepa Kunwar

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Materials ◽

Diamond Bur ◽

Retrograde Filling ◽

Significant Difference ◽

Supernatant Solution

Abstract Background The present study compares the apical microleakage of three different root-end filling materials in which the retrograde cavity is prepared by two different burs.Methods Eighty extracted single rooted maxillary and mandibular premolars were taken. Root canal treatment was completed. Apical 3 mm of all the teeth were resected with diamond disk. The tooth were divided into four groups with two subgroups for each group containing 10 tooth (N= 10) as: Group IA (Negative Control and IB (Positive Control); Group IIA and IIB: Prepared with round carbide bur and round diamond bur respectively, filled with GIC; Group IIIA and IIIB: Prepared with round carbide bur and round diamond bur respectively, filled with MTA; Group IVA and IVB: Prepared with round carbide bur and round diamond bur, filled with Biodentine. After applying two coats of nail varnish leaving apical 3 mm (except for negative control group) all teeth were immersed in 2% methylene blue for 3 days and again in 65% nitric acid for next 3 days for extraction of dye. The obtained solution was then transferred to eppendorf tube and centrifuged in microcentrifuges at 14,000 revolution per minutes (RPM) for 5 minutes. Optical density or absorbance of the supernatant solution was measured with UV spectrophotometer at 550 nm.Results The absorbance of the supernatant solution after dye extraction is decreasing in the order of positive control> GIC> MTA> Biodentine> negative control group. The significant difference was observed between GIC and MTA (p=0.0001) and GIC and Biodentine (p=0.0001) with two different burs but statistically non-significant difference was observed between MTA and Biodentine with Carbide bur (p=0.127) and Diamond bur (p=0.496) respectively.Conclusions Within the limitations of the present study, it can be concluded that Biodentine and MTA showed less microleakage as compared to GIC. There is no significant difference between mean microleakage of MTA and Biodentine. However, the mean OD of the Biodentine was least of all evaluated materials. Preparation of the root-end using round carbide bur as well as round diamond burs showed comparable microleakage for all three filling materials.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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Evaluation of the Sealing Ability of Three Obturation Techniques Using a Glucose Leakage Test

BioMed Research International ◽

10.1155/2017/2704094 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Katarzyna Olczak ◽

Halina Pawlicka

Keyword(s):

Continuous Wave ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Thermal Methods ◽

Root Canals ◽

Anterior Teeth ◽

Sealing Ability ◽

Significant Difference

The aim of this study was to evaluate the sealing ability of three different canal filling techniques. Sixty-four roots of extracted human maxillary anterior teeth were prepared using ProTaper® rotary instruments. The specimens were then randomly divided into 3 experimental groups (n=16) and 2 control groups (n=8). The root canals were filled using cold lateral compaction (CLC group), continuous wave condensation technique using the Elements Obturation Unit® (EOU group), and ProTaper obturators (PT group). For the negative control group, 8 roots were filled using lateral compaction as in the CLC group, and the teeth were covered twice with a layer of nail varnish (NCG group). Another 8 roots were filled using lateral compaction, but without sealer, and these were used as the positive control (PCG group). A glucose leakage model was used for quantitative evaluation of microleakage for 24 hours and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks. No significant difference in the cumulative amount of leakage was found between the three experimental groups at all observation times. The lateral condensation of cold gutta-percha can guarantee a similar seal of canal fillings as can be achieved by using thermal methods, in the round canals.

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Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

Hang Tuah Medical journal ◽

10.30649/htmj.v18i1.239 ◽

2020 ◽

Vol 18 (1) ◽

pp. 47

Author(s):

FERIZAL NEGERI SAMUDRA ◽

RETNO BUDIARTI ◽

IRMAWATI IRMAWATI

Keyword(s):

Sea Cucumber ◽

Antibacterial Effect ◽

Positive Control ◽

Negative Control ◽

Shigella Dysenteriae ◽

Control Group ◽

Control Groups ◽

Shigella Spp ◽

Diarrhea Disease

ABSTRACTBackground; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on Shigella Dysentriae growth in vitro is needed.Objectives: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the Shigella dysentriae bacteria in vitro.Method: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.Result: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.Conclusion: Sea cucumber (Holoturia scabra) inhibit the growth of Shigella dysenteriae.Key words: Shigella dysenteriae, sea cucumber (Holoturia scabra), antibacterial

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Antibacterial Effect of Silver Nanoparticles on Klebsiella spp

Healthcare Review ◽

10.47285/hr.v1i2.54 ◽

2020 ◽

Vol 1 (2) ◽

pp. 8-15

Author(s):

Gislanne Stéphanne Estevam da Silva ◽

Rivaldo Leon Bezerra Cabral ◽

Nathalie de Sena Pereira ◽

José Heriberto Oliveira do Nascimento ◽

Dany G kramer

Keyword(s):

Silver Nanoparticles ◽

Bacterial Resistance ◽

Brain Heart Infusion ◽

Trisodium Citrate ◽

Antimicrobial Activities ◽

Positive Control ◽

Negative Control ◽

In Vitro Tests ◽

Klebsiella Spp

Silver nanoparticles (AgNP) can be incorporated into medical devices, such as tissues, to circumvent bacterial resistance such as Klebsiella spp, which can lead to skin and mucosal infections. Thus, the aim of the present study was to synthesize silver nanoparticles for later incorporation into cotton fabrics and in vitro tests against Klebsiella spp. The AgNP colloidal solution was synthesized (AgNO3 - 0.1 mM, 100 mM trisodium citrate, polyvinylpyrrolidone - 0.24 g, H2OH2) and then impregnated into the cotton fabric pretreated with poly diallyl dimethylammonium chloride (PDDA) of 100/500 tissue, shaken for 30 minutes). The material produced was analyzed by the FTIR; DLS and reflectance spectroscopy. The tests of the antimicrobial activities were by the microdilution technique against Klebsiella spp, in tubes containing Brain Heart Infusion (BHI), with the solution of silver (1); Tissue containing AgNP - 4 mm (2); Negative control (3) and positive control - ceftriaxone (4). Regarding MIC, the inhibitory activity occurred of the dilutions between 1/2 and 1/16. The AgNP particles had an average size of 24.75 nm. As synthesized AgNPs demonstrate the excellent antimicrobial activity against Klebsiella spp, with special emphasis on applications in nanotechnology and nanomedicine, targeting multiresistant antibiotic bacteria.

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