scholarly journals Pemberian Krim Ekstrak Daun Sukun (Artocarpus altilis )Mencegah Peningkatan Jumlah Melanin Kulit Marmut (Cavia porcellus )yang Dipapar Sinar Ultraviolet B (UVB )

2018 ◽  
Vol 9 (2) ◽  
pp. 069
Author(s):  
Marisa Riliani ◽  
Wimpie Pangkahila ◽  
Wiraguna AAGP

<p>Skin is the main target of ultraviolet (UV) ray that result the abnormality of hiperpigmentation. In pathology, hyperpigmentation is caused by an increasing in the amount of melanin. The purpose of this research was to prove the effect of administration of breadfruit leaves extract (Artocarpus altilis) cream prevented the increase of skin melanin in guinea pig (Cavia procellus) exposed to ultraviolet B and to prove the administration of breadfruit leaves extract (Artocarpus altilis) cream have the same effectiveness with 4% hydroquinone cream in preventing the increase of skin melanin in guinea pig (Cavia procellus) exposed to ultraviolet B. This study was a true experimental research using post test only control group design. The subjects were devided into three groups. Group 1 as a control group was treated by UVB exposure and basic cream. Group 2 was treated by UVB and 4% hydroquinone cream. Group 3 was treated by UVB and 3% breadfruit leaves extract cream. There was significant difference within control group compared with group 2 and 3 (p&lt;0,05). There was no significant difference within group 2 compared with group 3 (p&gt;0,05).The administration of 3% breadfruit leaves extract (Artocarpus altilis) cream prevented the increase of skin melanin in guinea pig (Cavia procellus) exposed to ultraviolet B. The administration of 3% breadfruit leaves extract (Artocarpus altilis<br />) cream had the same effectiveness with 4% hydroquinone cream.</p>

2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Corazon H. Dumaria ◽  
A AGP Wiraguna ◽  
Wimpie Pangkahila

Abstract: This study was aimed to prove that administration of 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB and to compare this effect with 4% hydroquinone cream. This was a true experimental study using post test only control group design. Subjects were devided into three groups; each of 10 guinea pigs. Group 1, the control group, was exposed to UVB and applied with basic cream. Group 2 was exposed to UVB and applied with 4% hydroquinone cream. Group 3 was exposed to UVB and applied with 10% redfruit extract cream. The total dose of UVB was 390 mJ/cm2 given for 2 weeks. The amount of melanin was calculated using the percentage of the pixel area of melanin and was compared with the pixel areas of all epidermal tissues. The results showed that the highest percentage of melanin area was in group 1 (19.78%±3.79%). The percentage of melanin area in group 3 was 1.25%±0.76% meanwhile in group 2 was 0.85%±0.37%. There were signi-ficant differences in melanin percentage between the control group and group 2 as well as group 3 (P <0.05). There was no significant difference in melanin percentage between group 2 and group 3 (P >0.05). Conclusion: The 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB as effectively as the 4% hydroquinone cream.Keywords: redfruit extract cream, melanin, UVBAbstrak: Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak buah merah 10% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB dan perbandingannya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Sampel terdiri dari tiga kelompok dengan jumlah sampel 10 ekor marmut jantan tiap kelompok. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan diolesi krim hidrokuinon 4%. Kelompok 3 diberi paparan sinar UVB dan diolesi krim ekstrak buah merah 10%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (19,78± 3,79%) dan terendah pada kelompok 2 (0,85±0,37%), sedangkan jumlah melanin pada kelompok 3 di antara keduanya (1,25±0,76%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Tidak terdapat perbedaan bermakna antara kelompok 2 dan 3 dalam mencegah peningkatan jumlah melanin (P >0,05 ). Simpulan: Krim ekstrak buah merah 10% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB sama efektif dengan krim hidrokuinon 4%.Kata kunci: krim ekstrak buah merah, melanin, UVB


2017 ◽  
Vol 6 (2) ◽  
pp. 6
Author(s):  
Nurmawanti Nurmawanti ◽  
Ayly Soekanto

The aim of this research is to know the influence of anti mosquito electric gas that consisted Allethrin to the weight and colour of the rats liver. This research used an experimental method with the post test only  control group design.  The subject of this research is male rats weight of 150 grams each and total there was 24 rats that were divided to 4 groups. The first one, the  control groups (PO) was not given any of the gas, the second one, group 1 (P1) was given the gas for 4 hours everday, the third one, group  2 (P2), was given for 6 hours and last, group 3  (P3) was given 8 hours everyday. After later, on  30 Th day, the rats were terminated and being put in a surgery to remove their liver. This data was analyzed using SPPS for windows version 16. To see the differences in weight between the groups, it was analyzed using anova, and  to obtain the discoloration of the rats liver was analyzed using Kruskal Wallis Test. From the statistic tests, it show that there is significant difference in weight and color of liver in the group that α ≤ 0,05. According Anova Test, it shows that there is a significant difference α = 0,034 and from Kruskal Wallis test α = 0,013. In the conclusion anti mosquito electric gas that consist Allethrin affects the weight and the color of rats liver.


2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Rahmi Sofiana ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila

Abstract: Ultraviolet B (UVB) ray may cause skin hyperpigmentation due to increased melanin level. Noni seeds (Morinda citrifolia) extract was a strong candidate as an antioxidant and whitening agent. This study was aimed to prove the effect of noni seeds extract cream in prevention the increase of melanin in UVB-exposed guinea pig (Cavia porcellus). This was a true experimental study using posttest only control group design. Subjects were 30 guinea pigs divided into 3 groups, each of 10 guinea pigs. Group 1, the control group, was treated with UVB exposure and basic cream; group 2 was treated with UVB and 4% noni seed cream; and group 3 was treated with UVB and 4% hydroquinone cream. A total of 390 mJ/cm2 ultraviolet B dosage was given for 2 weeks. Histopathological examination with Masson-Fontana staining was performed to evaluate the melanin areas with black color. The amount of melanin was calculated by the percentage of pixel areas of melanin and was compared with the pixels of all epidermal tissues. The results showed that the highest number of melanin was in group 1 (10.61±5.33%), while in group 2 was 1.4±0.65%, and in group 3 was 0.45±0.23%. There was a significant difference between the control group and group 2 as well as group 3 (P <0.05) whereas no significant difference was found between group 2 and group 3 (P >0.05). Conclusion: The 4% noni seeds extract (Morinda citrifolia) cream could prevent the increase of skin melanin in UVB-exposed guinea pig as effective as 4% hydroquinone cream.Keywords: noni seeds extract cream, melanin, ultraviolet B, guinea pigs Abstrak: Paparan sinar ultraviolet B (UVB) mengakibatkan terjadinya kelainan hiperpigmentasi yang ditandai dengan peningkatan jumlah melanin. Ekstrak biji mengkudu memiliki kombinasi zat aktif yang dapat bekerja sinergis dalam mencegah peningkatan jumlah melanin. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak biji mengkudu (Morinda citrifolia) dalam mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar UVB. Jenis penelitian ialah eksperimental dengan randomized posttest only control group design menggunakan 30 ekor marmut jantan yang dibagi atas 3 kelompok, masing-masing 10 ekor. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan krim ekstrak biji mengkudu 4%. Kelompok 3 diberi paparan sinar UVB dan krim hidrokuinon 4%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Pemeriksaan histopatologik jaringan kulit dengan pewarnaan Masson- Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin pada Kelompok 1 sebesar 10,61±5,33%; pada kelompok 2 sebesar 1.4±0.65%, dan pada kelompok 3 sebesar 0,45±0,23%. Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Perbandingan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak biji mengkudu 4% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB, dan memiliki efektifitas yang sama dengan krim hidrokuinon 4%. Kata kunci: krim ekstrak biji mengkudu, melanin, ultraviolet B, marmut


2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Putri Puspitasari ◽  
AAG Wiraguna ◽  
Wimpie Pangkahila

Abstract: This study was aimed to evaluate the effectiveness of 20% green tea extract in preventing the increase of melanin in guinea pig skin exposed to UV-B and to compare the effectiveness of this treatment to 4% hydroquinone cream. This was an experimental study with the post-test only control group design. Subjects were four groups; each consisted of 7 male guinea pigs. Group 1, the control group, was exposed to UV-B only. Group 2 was exposed to UV-B and applied with cream based substance. Group 3 was exposed to UV-B and applied with 4% hydroquinone cream. Group 4 was exposed to UV-B and applied with 20% green tea extract. The total UV-B intensity was 390 mJ/cm2 consistently exposed for two weeks. Histopathological slides of the skin tissue were stained with Masson Fontana technique. The total amount of melanin was calculated in percentage of pixel compared to pixel of melanin in total epidermis. The results showed that the highest amount of melanin was in group 1 (24.44%) and the lowest amount of melanin was in group 3 (1.04%) meanwhile the amount of melanin in group 4 (1.34%) was nearly the same with group 3. There were significant differences between group 1 to group 3 and 4 (P < 0.05). There was no significant difference between group 3 and group 4 (P > 0.05). Conclusion: The 20% green tea extract was as effective as 4% hydroquinone cream in inhibiting the increase of melanin in guinea pigs’skin exposed to UV-B.Keywords: green tea extract, melanin, ultraviolet BAbstrak: Penelitian ini bertujuan untuk mengetahui efek pemberian krim ekstrak teh hijau 20% terhadap peningkatan jumlah melanin pada kulit marmut yang dipajan sinar UVB serta membandingkan efektivitasnya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian terdiri dari empat kelompok dengan 7 ekor marmut jantan setiap kelompok. Kelompok 1 (kelompok kontrol) diberi pajanan sinar UV-B saja. Kelompok 2 diberi pajanan sinar UV-B dan diolesi krim dasar. Kelompok 3 diberi pajanan sinar UV-B dan krim hidrokuinon 4% sedangkan kelompok 4 diberi pajanan sinar UV-B dan krim ekstrak teh hijau 20%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit dipulas dengan pewarnaan Masson Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (24,44%) dan terendah pada kelompok 3 (1,04%). Jumlah melanin pada kelompok 4 (1,34%) hampir sama dengan kelompok 3. Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 3 dan 4 (P < 0,05). Perbandingan antara kelompok 3 dan 4 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P > 0,05). Simpulan: Pemberian krim ekstrak teh hijau 20% sama efektif dengan krim hidrokuinon 4% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipajan sinar UV-B.Kata kunci: krim ekstrak teh hijau, melanin, ultraviolet B


2021 ◽  
Vol 4 (1) ◽  
pp. 22-27
Author(s):  
Chynthia Friama ◽  
Anak Agung Gde Putra Wiraguna ◽  
Wimpie Pangkahila

Background: Excessive sun exposure results in increased activity of the tyrosinase enzyme and the amount of melanin in the skin which causes hyperpigmentation, a sign of premature aging. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin and tyrosinase enzymes as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin. Methods: This study used a randomized post-test only control group design. The subjects were 36 male guinea pigs (Cavia porcellus). Subjects were divided into two groups, the hydroquinone cream 4% group and the Ashitaba leaves extract cream 8% group exposed to UVB light. Skin samples were collected 48 hours after the last exposure to measure the tyrosinase enzyme levels using the ELISA method and the amount of melanin was examined by Masson-Fontana staining. Results: The mean levels of tyrosinase in the hydroquinone cream 4% group was 19.51 ± 5.16 ng/L and the Ashitaba leaves extract cream 8% group was 23.76 ± 3.09 ng/L (p = 0.005). The mean amount of melanin in the  hydroquinone cream 4% group was 2.98 ± 2.27% and the Ashitaba leaves extract cream 8% group was 4.77 ± 3.33% (p = 0.069). Conclusion: The administration of Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin.


2020 ◽  
Vol 2 (1) ◽  
pp. 32
Author(s):  
Ahmad Farham Majid ◽  
Ismail Ismail ◽  
Mardhiah Mardhiah ◽  
Fitriani Nur

AbstrakPenelitian ini bertujuan untuk mengetahui kemampuan komunikasi matematis siswa yang menggunakan metode silih tanya berbantuan kartu model dan metode make a match. Jenis penelitian ini adalah quasi eksperimen dengan desain penelitian non equivalent pretest-posttest control group design. Populasi dalam penelitian ini adalah seluruh siswa kelas VII SMPN 4 Sungguminasa Kab. Gowa berjumlah 351 siswa dan total sampel berjumlah 64 siswa dengan teknik pengambilan sampel purposive sampling. Instrumen yang digunakan dalam penelitian ini adalah tes yang terdiri dari pretest dan posttest dan non tes berupa lembar observasi. Berdasarkan hasil analisis data nilai siswa menggunakan statistik deskriptif rata-rata nilai kemampuan komunikasi matematis siswa yang diajar menggunakan metode silih tanya berbantuan kartu model adalah 86,84 dengan kategori sedang dan yang diajar menggunakan metode make a match adalah 77,78 dengan kategori sedang. Berdasarkan analisis statistik inferensial bahwa terdapat perbedaan yang signifikan antara kemampuan komunikasi matematis siswa yang menggunakan metode silih tanya berbantuan kartu model dengan yang menggunakan metode make a match pada kelas VII SMPN 4 Sungguminasa Kab. Gowa. AbstractThis study aims to determine the mathematical communication ability of students who use card-assisted questions method and make a match method. This type of research is a quasi-experimental research with nonequivalent pretest-posttest control group design. The population in this study were all VII grade students of SMPN 4 Sungguminasa, Gowa Regency with 351 students and a total sample of 64 students with purposive sampling techniques. The instrument used in this study was a test consisting of pre-test and post-test and non-test that is observation sheet. Based on the data analysis results that average the value of students’ mathematical communication skills who were taught using the card-assisted questions was 86.84 in the medium category and those who taught using make a match method were 77.78 in the medium category. The results of inferential statistical analysis that there is a significant difference between the mathematical communication abilities of students who use card-assisted questions and using the make a match method in class VII of SMPN 4 Sungguminasa, Gowa Regency.


2021 ◽  
Vol 9 (3) ◽  
pp. 712-719
Author(s):  
Ayşe Eliüşük Bülbül ◽  

The purpose of this study is to determine the effect of the "patience training program" on the patience and life satisfaction levels of university students. The study was organized as a pre-test, post-test experiment and control group design. For a total of 30 students from which were 17 in the control group and 17 in the experimental group, patience training was given for 5 weeks. The "Patient Scale" developed by Schnitker (2010) and adapted to Turkish by Eliüşük and Arslan (2016) and the "Life Satisfaction Scale" developed by Diener, Emmons, Larsen and Griffin (1985) and adapted to Turkish by Dağlı and Baysal (2016) were used as a data collection tool in the study. The "Wilcoxon" test was used for the comparison of in-group differences in the analysis of obtained data and the "Mann-Whitney U" test was used in examining the differences between the two groups. As a result of the study, it was observed that the patience and life satisfaction average scores of students in the experimental group receiving "patience training" increased significantly, while there was no significant difference in the patience and life satisfaction averages scores of the control group students.


2018 ◽  
Vol 24 (1) ◽  
pp. 37
Author(s):  
Annisa Trissatharra ◽  
Sri Ratna Dwiningsih ◽  
Ratna Sofaria Munir

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.


2019 ◽  
Vol 2 (2) ◽  
pp. 269
Author(s):  
Yoga Pratama

The objectives of this study were to find out whether or not there was significant improvement in writing and speaking achievements of the eighth grade students of SMPN1 Jarai after they were taught by using Sociogram, to find out whether or not there was significant improvement in each aspect of writing and speaking achievements of the eighth grade students of SMPN1 Jarai after they were taught by using Sociogram, to find out whether or not there was significant difference in writing and speaking achievements between the eighth grade students of SMP 1 Jarai who were taught by using Sociogram and those who were not, and to find out the perception of the eighth grade students of SMPN 1 Jarai after they were taught by using Socoiogram. In this paper, an experimental research method with pre and post test control group design was used with 70 students as the sample divided into two groups, experimental and control group. However, only the students of the experimental group were taught by using Sociogram. The obtained data were analyzed by using t-test. The students were given questionnaire to know their perceptions. The result showed that there was a significant difference in both students? writing and speaking achievements after they were tought by using Sociogram. Furthermore, it was found that there was a significant difference in students? writing and speaking achievement between the experimental and control groups. In conclusion, Sociogram improves the students? writing and speaking achievements.


2018 ◽  
Vol 21 (1) ◽  
pp. 26 ◽  
Author(s):  
Hatice Miray Uyan ◽  
Keziban Olcay ◽  
Mutlu Özcan

<p><strong>Objective:</strong> This study was designed to evaluate postoperative pain after endodontic retreatment. <strong>Material and Methods:</strong> Asymptomatic, multi-rooted molar &amp; premolar teeth requiring retreatment with 2–5 mm periapical lesions were included. Seventy-eight teeth were randomly placed in four groups (n=20): single-visit (control, group 1), Ledermix (group 2), metronidazole, ciprofloxacin, minocycline mixture (group 3), calcium hydroxide (group 4). The postoperative pain was recorded using a VAS at 6, 12, 24, and 48 h after retreatment. Statistical evaluation was performed using Two-Way Repeated Measures ANOVA and Tukey test. <strong>Results:</strong> Mild pain occurred in 67.5%, moderate in 30%, and flare-ups in 2.5%, and there was a significant difference between the groups (p&lt;0.01). Significantly lower postoperative pain was observed in TAP and CaOH<sub>2 </sub>groups(p&lt;0.05). In the 6, 12, and 24 h intervals, there was a significant difference in the pain levels (p&lt;0.05). <strong>Conclusion:</strong> TAP and CaOH<sub>2</sub> are effective for reducing postoperative pain after retreatment.</p><p><strong>Keywords</strong></p><p>Intracanal medicaments; Multiple-visit; Postoperative pain; Retreatment; Single-visit.</p>


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