scholarly journals Krim Ekstrak Buah Merah (Pandanus conoideus ) 10% Sama Efektifnya dengan Krim Hidrokuinon 4% dalam Mencegah Peningkatan Jumlah Melanin Kulit Marmut (Cavia porcellus) yang Dipapar Sinar Ultraviolet B

2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Corazon H. Dumaria ◽  
A AGP Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Guinea Pig ◽  
Ultraviolet B ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract: This study was aimed to prove that administration of 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB and to compare this effect with 4% hydroquinone cream. This was a true experimental study using post test only control group design. Subjects were devided into three groups; each of 10 guinea pigs. Group 1, the control group, was exposed to UVB and applied with basic cream. Group 2 was exposed to UVB and applied with 4% hydroquinone cream. Group 3 was exposed to UVB and applied with 10% redfruit extract cream. The total dose of UVB was 390 mJ/cm2 given for 2 weeks. The amount of melanin was calculated using the percentage of the pixel area of melanin and was compared with the pixel areas of all epidermal tissues. The results showed that the highest percentage of melanin area was in group 1 (19.78%±3.79%). The percentage of melanin area in group 3 was 1.25%±0.76% meanwhile in group 2 was 0.85%±0.37%. There were signi-ficant differences in melanin percentage between the control group and group 2 as well as group 3 (P <0.05). There was no significant difference in melanin percentage between group 2 and group 3 (P >0.05). Conclusion: The 10% redfruit extract cream could prevent the increase of skin melanin in guinea pig exposed to UVB as effectively as the 4% hydroquinone cream.Keywords: redfruit extract cream, melanin, UVBAbstrak: Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak buah merah 10% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB dan perbandingannya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Sampel terdiri dari tiga kelompok dengan jumlah sampel 10 ekor marmut jantan tiap kelompok. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan diolesi krim hidrokuinon 4%. Kelompok 3 diberi paparan sinar UVB dan diolesi krim ekstrak buah merah 10%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (19,78± 3,79%) dan terendah pada kelompok 2 (0,85±0,37%), sedangkan jumlah melanin pada kelompok 3 di antara keduanya (1,25±0,76%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Tidak terdapat perbedaan bermakna antara kelompok 2 dan 3 dalam mencegah peningkatan jumlah melanin (P >0,05 ). Simpulan: Krim ekstrak buah merah 10% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB sama efektif dengan krim hidrokuinon 4%.Kata kunci: krim ekstrak buah merah, melanin, UVB

scholarly journals Pemberian Krim Ekstrak Daun Sukun (Artocarpus altilis )Mencegah Peningkatan Jumlah Melanin Kulit Marmut (Cavia porcellus )yang Dipapar Sinar Ultraviolet B (UVB )

2018 ◽  
Vol 9 (2) ◽  
pp. 069
Author(s):  
Marisa Riliani ◽  
Wimpie Pangkahila ◽  
Wiraguna AAGP
Keyword(s):  
Guinea Pig ◽  
Ultraviolet B ◽  
Control Group ◽  
Control Group Design ◽  
Artocarpus Altilis ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
Uvb Exposure ◽  
Group 2

<p>Skin is the main target of ultraviolet (UV) ray that result the abnormality of hiperpigmentation. In pathology, hyperpigmentation is caused by an increasing in the amount of melanin. The purpose of this research was to prove the effect of administration of breadfruit leaves extract (Artocarpus altilis) cream prevented the increase of skin melanin in guinea pig (Cavia procellus) exposed to ultraviolet B and to prove the administration of breadfruit leaves extract (Artocarpus altilis) cream have the same effectiveness with 4% hydroquinone cream in preventing the increase of skin melanin in guinea pig (Cavia procellus) exposed to ultraviolet B. This study was a true experimental research using post test only control group design. The subjects were devided into three groups. Group 1 as a control group was treated by UVB exposure and basic cream. Group 2 was treated by UVB and 4% hydroquinone cream. Group 3 was treated by UVB and 3% breadfruit leaves extract cream. There was significant difference within control group compared with group 2 and 3 (p&lt;0,05). There was no significant difference within group 2 compared with group 3 (p&gt;0,05).The administration of 3% breadfruit leaves extract (Artocarpus altilis) cream prevented the increase of skin melanin in guinea pig (Cavia procellus) exposed to ultraviolet B. The administration of 3% breadfruit leaves extract (Artocarpus altilis<br />) cream had the same effectiveness with 4% hydroquinone cream.</p>

scholarly journals The Effect of Anti Masquito Electric Gas that Consist Allethrin That Influence The Weight and Colour of Rats Liver

2017 ◽  
Vol 6 (2) ◽  
pp. 6
Author(s):  
Nurmawanti Nurmawanti ◽  
Ayly Soekanto
Keyword(s):  
Male Rats ◽  
Control Group ◽  
Control Groups ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
The Subject ◽  
Group 2 ◽  
Group 1

The aim of this research is to know the influence of anti mosquito electric gas that consisted Allethrin to the weight and colour of the rats liver. This research used an experimental method with the post test only  control group design.  The subject of this research is male rats weight of 150 grams each and total there was 24 rats that were divided to 4 groups. The first one, the  control groups (PO) was not given any of the gas, the second one, group 1 (P1) was given the gas for 4 hours everday, the third one, group  2 (P2), was given for 6 hours and last, group 3  (P3) was given 8 hours everyday. After later, on  30 Th day, the rats were terminated and being put in a surgery to remove their liver. This data was analyzed using SPPS for windows version 16. To see the differences in weight between the groups, it was analyzed using anova, and  to obtain the discoloration of the rats liver was analyzed using Kruskal Wallis Test. From the statistic tests, it show that there is significant difference in weight and color of liver in the group that α ≤ 0,05. According Anova Test, it shows that there is a significant difference α = 0,034 and from Kruskal Wallis test α = 0,013. In the conclusion anti mosquito electric gas that consist Allethrin affects the weight and the color of rats liver.

scholarly journals Krim ekstrak etanol biji mengkudu (Morinda citrifolia) sama efektifnya dengan krim hidrokuinon dalam mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar ultraviolet B

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Rahmi Sofiana ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Guinea Pigs ◽  
Ultraviolet B ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Cavia Porcellus ◽  
Control Group Design ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract: Ultraviolet B (UVB) ray may cause skin hyperpigmentation due to increased melanin level. Noni seeds (Morinda citrifolia) extract was a strong candidate as an antioxidant and whitening agent. This study was aimed to prove the effect of noni seeds extract cream in prevention the increase of melanin in UVB-exposed guinea pig (Cavia porcellus). This was a true experimental study using posttest only control group design. Subjects were 30 guinea pigs divided into 3 groups, each of 10 guinea pigs. Group 1, the control group, was treated with UVB exposure and basic cream; group 2 was treated with UVB and 4% noni seed cream; and group 3 was treated with UVB and 4% hydroquinone cream. A total of 390 mJ/cm2 ultraviolet B dosage was given for 2 weeks. Histopathological examination with Masson-Fontana staining was performed to evaluate the melanin areas with black color. The amount of melanin was calculated by the percentage of pixel areas of melanin and was compared with the pixels of all epidermal tissues. The results showed that the highest number of melanin was in group 1 (10.61±5.33%), while in group 2 was 1.4±0.65%, and in group 3 was 0.45±0.23%. There was a significant difference between the control group and group 2 as well as group 3 (P <0.05) whereas no significant difference was found between group 2 and group 3 (P >0.05). Conclusion: The 4% noni seeds extract (Morinda citrifolia) cream could prevent the increase of skin melanin in UVB-exposed guinea pig as effective as 4% hydroquinone cream.Keywords: noni seeds extract cream, melanin, ultraviolet B, guinea pigs Abstrak: Paparan sinar ultraviolet B (UVB) mengakibatkan terjadinya kelainan hiperpigmentasi yang ditandai dengan peningkatan jumlah melanin. Ekstrak biji mengkudu memiliki kombinasi zat aktif yang dapat bekerja sinergis dalam mencegah peningkatan jumlah melanin. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak biji mengkudu (Morinda citrifolia) dalam mencegah peningkatan jumlah melanin kulit marmut (Cavia porcellus) yang dipapar sinar UVB. Jenis penelitian ialah eksperimental dengan randomized posttest only control group design menggunakan 30 ekor marmut jantan yang dibagi atas 3 kelompok, masing-masing 10 ekor. Kelompok 1 yaitu kelompok kontrol, diberi paparan sinar UVB dan diolesi krim dasar. Kelompok 2 diberi paparan sinar UVB dan krim ekstrak biji mengkudu 4%. Kelompok 3 diberi paparan sinar UVB dan krim hidrokuinon 4%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Pemeriksaan histopatologik jaringan kulit dengan pewarnaan Masson- Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin pada Kelompok 1 sebesar 10,61±5,33%; pada kelompok 2 sebesar 1.4±0.65%, dan pada kelompok 3 sebesar 0,45±0,23%. Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05). Perbandingan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak biji mengkudu 4% dapat mencegah peningkatan jumlah melanin kulit marmut yang dipapar sinar UVB, dan memiliki efektifitas yang sama dengan krim hidrokuinon 4%. Kata kunci: krim ekstrak biji mengkudu, melanin, ultraviolet B, marmut

scholarly journals Utilization of Artocarpus heterophylla Lamk. As UV Protector In mice exposed to UV-B

2018 ◽  
Vol 23 (3) ◽  
pp. 112
Author(s):  
Ketut Agus Adrianta ◽  
I Made Agus Sunadi Putra
Keyword(s):  
Leaf Extract ◽  
Animal Feed ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Group 3 ◽  
Collagen Density ◽  
Group 2 ◽  
Group 1

In daily life, Artocarpus heterophylla Lamk., the leaves are rarely used even just as animal feed. In the bark of Artocarpus heterophylla Lamk there are new flavonoid compounds namely morusin, artokarpin, artonin E, cycloartobilosantone, and artonol B. This research is an experimental design with randomized posttest only with control group design. A total of 21 mice were divided into 3 groups, each consisting of 7 mice, group 1: negative control given oral placebo and exposed to UV-B rays, group 2:giving Vitamin C cream 4% mg and exposed to UV-B rays, group 3: giving cream of Artocarpus heterophylla Lamk leaf extract 4% and exposed to UV-B rays. The total of UV radiation dose of 600 mJ / cm² for 3 weeks, then performed a biopsy for examination of the amount of dermis collagen. The average number of collagen in the three groups after treatment was given significantly different (p <0.05). Mean and Significant level of collagen density of group 1 : 51,42 ± 5,16; group P2 ± 91,18 ± 1,13; and P3 group: 88,77 ± 1,10. Conclusion : Cream of 4% Artocarpus heterophylla Lamk leaf extract can increase the amount of collagen density in mice exposed to UV-B rays.

scholarly journals Pengaruh pemberian kopi terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang terpapar stres

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Risnawati A. Haris ◽  
Lydia Tendean ◽  
Grace Turalaki
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Group 2 ◽  
Light Form ◽  
Group 1

Abstract: The coffee beans composed of the compound caffeine, palmitic acid, linoleic acid and acid stearic. The content of caffeine in coffee can improve spermatozoa motility that are not motile by inhibiting cyclic nucleotide phosphodiesterase and affects the intracellular levels of cyclic AMP. Spermatozoa quality can be affected by various factors such as stress. In nocturnal animals, dark periode becomes a signal to do activity by suprachiasmatic nucleus. The change in activity of the nocturnal animals from initial condition in the dark then exposed to light can leads to stress. This study was aimed to determine the effect of coffee on the spermatozoa quality of wistar rats which had been exposed to stress. This was an experimental analytical study with a post-test only control group design. The study was conducted for 50 days from September to November 2016. The sample consisted of 9 rats divided into three groups: a control group (P0) is given only exposure to stress in the form of light, the treatment group 1 (P1) is given exposure to stress in the light form and liquid coffee 1 ml / kg and the treatment group 2 (P2) is given exposure to stress in the light form and coffee extracts. The results showed that there were significant differences in the concentration and motility treatment group 1 (P1) to the control group (P0) which is p <0.05 and significant differences in sperm motility treatment group 2 (P2) to the control group (P0) which is p <0.05. Conclusion: Coffee administration could improve the quality of spermatozoa of wistar rats exposed to stress.Keywords: stress, coffee, quality of spermatozoa. Abstrak: Biji kopi tersusun dari senyawa kafein, asam palmatik, asam linoleat dan asam stearik. Kandungan kafein yang terdapat didalam kopi mampu meningkatkan motilitas spermatozoa yang tidak motil dengan cara menghambat siklus nukleotida fosfodiesterase dan mempengaruhi level intraseluler dari siklus AMP. Kualitas spermatozoa juga dapat dipengaruhi oleh berbagai faktor lain seperti stres. Pada hewan nokturnal periode gelap menjadi sinyal untuk beraktiftas dilakukan oleh suprachiasmatic nucleus. Perubahan aktivitas pada hewan nokturnal dari kondisi awalnya ditempat yang gelap lalu diberikan paparan cahaya dapat menyebabkan terjadinya stres. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian kopi terhadap kualitas spermatozoa tikus wistar yang terpapar stres. Jenis penelitian ialah eksperimental analitik dengan post-test only control group design. Penelitian dilakukan selama 50 hari dari bulan September – November 2016. Sampel sebanyak 9 ekor tikus dibagi menjadi tiga kelompok yaitu : kelompok kontrol (P0) hanya diberikan paparan stres berupa cahaya, kelompok perlakuan 1 (P1) diberikan paparan stres berupa cahaya dan cairan kopi 1 mL/KgBb dan kelompok perlakuan 2 (P2) diberikan paparan stres berupa cahaya dan ekstrak kopi. Hasil penelitian menunjukkan adanya perbedaan yang signifikan pada konsentrasi dan motilitas spermatozoa kelompok perlakuan 1 (P1) terhadap kelompok kontrol (P0) yaitu p<0,05 dan perbedaan yang signifikan pada motilitas spermatozoa kelompok perlakuan 2 (P2) terhadap kelompok kontrol (P0) yaitu p<0,05. Simpulan: Pemberian kopi meningkatkan kualitas spermatozoa yang terpapar stres. Kata kunci: stres, kopi, kualitas spermatozoa

scholarly journals Krim ekstrak teh hijau 20% (Camellia sinensis) mencegah peningkatan jumlah melanin sama efektif dengan krim hidrokuinon 4% pada kulit marmut (Cavia porcellus) yang dipajan sinar ultraviolet B

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Putri Puspitasari ◽  
AAG Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Green Tea ◽  
Green Tea Extract ◽  
Ultraviolet B ◽  
Control Group ◽  
Control Group Design ◽  
Group 4 ◽  
Post Test ◽  
Group 3 ◽  
Tea Extract ◽  
Group 1

Abstract: This study was aimed to evaluate the effectiveness of 20% green tea extract in preventing the increase of melanin in guinea pig skin exposed to UV-B and to compare the effectiveness of this treatment to 4% hydroquinone cream. This was an experimental study with the post-test only control group design. Subjects were four groups; each consisted of 7 male guinea pigs. Group 1, the control group, was exposed to UV-B only. Group 2 was exposed to UV-B and applied with cream based substance. Group 3 was exposed to UV-B and applied with 4% hydroquinone cream. Group 4 was exposed to UV-B and applied with 20% green tea extract. The total UV-B intensity was 390 mJ/cm2 consistently exposed for two weeks. Histopathological slides of the skin tissue were stained with Masson Fontana technique. The total amount of melanin was calculated in percentage of pixel compared to pixel of melanin in total epidermis. The results showed that the highest amount of melanin was in group 1 (24.44%) and the lowest amount of melanin was in group 3 (1.04%) meanwhile the amount of melanin in group 4 (1.34%) was nearly the same with group 3. There were significant differences between group 1 to group 3 and 4 (P < 0.05). There was no significant difference between group 3 and group 4 (P > 0.05). Conclusion: The 20% green tea extract was as effective as 4% hydroquinone cream in inhibiting the increase of melanin in guinea pigs’skin exposed to UV-B.Keywords: green tea extract, melanin, ultraviolet BAbstrak: Penelitian ini bertujuan untuk mengetahui efek pemberian krim ekstrak teh hijau 20% terhadap peningkatan jumlah melanin pada kulit marmut yang dipajan sinar UVB serta membandingkan efektivitasnya dengan krim hidrokuinon 4%. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian terdiri dari empat kelompok dengan 7 ekor marmut jantan setiap kelompok. Kelompok 1 (kelompok kontrol) diberi pajanan sinar UV-B saja. Kelompok 2 diberi pajanan sinar UV-B dan diolesi krim dasar. Kelompok 3 diberi pajanan sinar UV-B dan krim hidrokuinon 4% sedangkan kelompok 4 diberi pajanan sinar UV-B dan krim ekstrak teh hijau 20%. Dosis total UVB yaitu 390 mJ/cm2 diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit dipulas dengan pewarnaan Masson Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan dengan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan jumlah melanin tertinggi pada kelompok 1 (24,44%) dan terendah pada kelompok 3 (1,04%). Jumlah melanin pada kelompok 4 (1,34%) hampir sama dengan kelompok 3. Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 3 dan 4 (P < 0,05). Perbandingan antara kelompok 3 dan 4 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P > 0,05). Simpulan: Pemberian krim ekstrak teh hijau 20% sama efektif dengan krim hidrokuinon 4% dalam mencegah peningkatan jumlah melanin kulit marmut yang dipajan sinar UV-B.Kata kunci: krim ekstrak teh hijau, melanin, ultraviolet B

PERBANDINGAN KEMAMPUAN KOMUNIKASI MATEMATIS SISWA YANG MENGGUNAKAN METODE SILIH TANYA BERBANTUAN KARTU MODEL DAN METODE MAKE A MATCH

2020 ◽  
Vol 2 (1) ◽  
pp. 32
Author(s):  
Ahmad Farham Majid ◽  
Ismail Ismail ◽  
Mardhiah Mardhiah ◽  
Fitriani Nur
Keyword(s):  
Total Sample ◽  
Control Group ◽  
Match Method ◽  
Mathematical Communication ◽  
Control Group Design ◽  
Purposive Sampling ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Quasi Experimental

AbstrakPenelitian ini bertujuan untuk mengetahui kemampuan komunikasi matematis siswa yang menggunakan metode silih tanya berbantuan kartu model dan metode make a match. Jenis penelitian ini adalah quasi eksperimen dengan desain penelitian non equivalent pretest-posttest control group design. Populasi dalam penelitian ini adalah seluruh siswa kelas VII SMPN 4 Sungguminasa Kab. Gowa berjumlah 351 siswa dan total sampel berjumlah 64 siswa dengan teknik pengambilan sampel purposive sampling. Instrumen yang digunakan dalam penelitian ini adalah tes yang terdiri dari pretest dan posttest dan non tes berupa lembar observasi. Berdasarkan hasil analisis data nilai siswa menggunakan statistik deskriptif rata-rata nilai kemampuan komunikasi matematis siswa yang diajar menggunakan metode silih tanya berbantuan kartu model adalah 86,84 dengan kategori sedang dan yang diajar menggunakan metode make a match adalah 77,78 dengan kategori sedang. Berdasarkan analisis statistik inferensial bahwa terdapat perbedaan yang signifikan antara kemampuan komunikasi matematis siswa yang menggunakan metode silih tanya berbantuan kartu model dengan yang menggunakan metode make a match pada kelas VII SMPN 4 Sungguminasa Kab. Gowa. AbstractThis study aims to determine the mathematical communication ability of students who use card-assisted questions method and make a match method. This type of research is a quasi-experimental research with nonequivalent pretest-posttest control group design. The population in this study were all VII grade students of SMPN 4 Sungguminasa, Gowa Regency with 351 students and a total sample of 64 students with purposive sampling techniques. The instrument used in this study was a test consisting of pre-test and post-test and non-test that is observation sheet. Based on the data analysis results that average the value of students’ mathematical communication skills who were taught using the card-assisted questions was 86.84 in the medium category and those who taught using make a match method were 77.78 in the medium category. The results of inferential statistical analysis that there is a significant difference between the mathematical communication abilities of students who use card-assisted questions and using the make a match method in class VII of SMPN 4 Sungguminasa, Gowa Regency.

scholarly journals Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

2018 ◽  
Vol 24 (1) ◽  
pp. 37
Author(s):  
Annisa Trissatharra ◽  
Sri Ratna Dwiningsih ◽  
Ratna Sofaria Munir
Keyword(s):  
Mus Musculus ◽  
Ovarian Follicles ◽  
Observation Time ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

Investigation of TAp63 gene Expression and Follicle Count Using Melatonin in Cisplatin-induced Ovarian Toxicity

2020 ◽  
Author(s):  
Hacı Öztürk Şahin ◽  
Mehmet Nuri Duran ◽  
Fatma Sılan ◽  
Ece Sılan ◽  
Duygu Sıddıkoglu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Female Rats ◽  
Histological Evaluation ◽  
Saline Control ◽  
Control Group ◽  
Ovarian Toxicity ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract Background: Premature ovarian failure is among the most important side effects of chemotherapy during reproductive period. Preserving ovarian function is gradually gaining importance during oncologic treatment. The present study aims to investigate the potential of melatonin to protect from cisplatin-induced ovarian toxicity in rats. Twenty nine female rats were divided to three groups: Saline control group (Group 1), cisplatin group (Group 2), and cisplatin+melatonin group (Group 3). While the rats in Groups 2 and 3 were administered 5 mg/kg single dose of cisplatin via intra-peritoneal (IP) route, the rats in Group 3 were started on melatonin (20 mg/kg IP) before cisplatin administration and continued during 3 consecutive days. Ovaries were removed one week after cisplatin administration in all groups. Blood samples were obtained before the rats were decapited. Histological evaluation, follicle count, and classification were performed. TAp63 mRNA expression was evaluated using mRNA extraction and real-time polymerase chain reaction (PCR) method. Serum estradiol (E2) and anti-mullerian hormone (AMH) values were measured with enzyme immune-assay technology. Results: While primordial follicles were seen to decrease in Group 2 as compared to Group 1 (p:0.023), primordial follicle count was observed to be preserved significantly in melatonin group as compared to Group 2 (p:0.047). Moreover, cisplatin-induced histo-pathological morphology was preserved in favor of normal histology in melatonin group. A significant difference was not observed between groups with regard to mean serum AMH and E2 values (p:0.102 and p:0.411, respectively). While TAp63 gene expression significantly increased in Group 2 as compared to control group (p:0.001), we did not detect a statistically significant difference in cisplatin+melatonin group, although gene expression decreased (p:0.34). Conclusion: We conclude that concurrent administration of melatonin and cisplatin may protect from ovarian damage.

Endovascular reflux-elimination of perforating veins by RFITT and foam sclerotherapy

Phlebologie ◽  
2015 ◽  
Vol 44 (01) ◽  
pp. 13-17
Author(s):  
J. Duben ◽  
J. Gatek ◽  
T. Saha ◽  
G. Hnatkova ◽  
L. Hnatek
Keyword(s):  
Varicose Veins ◽  
Control Group ◽  
Foam Sclerotherapy ◽  
Perforating Veins ◽  
Significant Difference ◽  
Power Setting ◽  
Group 3 ◽  
Group 2 ◽  
One Year ◽  
Group 1

SummaryIntroduction: During the last years, many endovascular techniques have been developed in order to eliminate not only the reflux in stem veins but in perforating veins and their tributaries, too.Aim: The aim of this study was to use endo -vascular RFITT and the foam sclerotherapy for the occlusion of perforating veins as the prime source of reflux and their tributaries.Material and Methods: The Celon method was used for the thermal treatment. Polydocalon with the concentration 1% and 2% with DSS technique was used for the foam sclerotherapy. The RFITT was accomplished in 127 perforating veins in total. This group was divided into three subgroups. The first one consists of patients where only RFITT was carried out (n= 41), in the second, there were patients with RFITT realized with sclerotherapy during one session (n= 48), in the third, RFITT was completed with sclerotherapy in one month after the RFITT intervention (n= 38). The control group included perforating veins treated only with sclerotherapy (n= 81). The power setting 6W was used on the generator during the RFITT with CelonProSurge micro and 18W for usage of Celon ProCurve probe.Results: The effectiveness of the procedure in the group 1 was 8.8%, in the group two 93.7%, in the group three 92.1% and in the control group 76.5% in one year follow up. There was no significant difference between the effectiveness in groups 1, 2 and 3. The marginal difference was among all three groups with RFITT and the control group. Significant differences were in the parameter of the extinction of visible varicose veins with the reflux from perforators. The extinction was faster in group 3 than in group 2 and in the control group and the slowest was in group 1. The significant difference was observed between groups 2 and 3 compared with group 1 and the margin difference was between groups 2 and 3 compared with the control group. No significant difference was observed between groups 1 and the control group.Conclusions: All procedures are effective. The most important is the combination of RFITT and the sclerotherapy one month after thermal intervention. This is associated with a low risk of recanalization and the fastest extinction of visible varicose veins.

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