scholarly journals Phytochemical screening and evaluation of antidepressant activity of methanolic extract of the spadix of Colocasia affinis Schott

2018 ◽  
Vol 18 (4) ◽  
pp. 104-110
Author(s):  
C. Lalremruati ◽  
C. Malsawmtluangi ◽  
H. Lalhlenmawia
Keyword(s):  
Methanolic Extract ◽  
Petroleum Ether Extract ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Chloroform Extract ◽  
Control Group ◽  
Force Swim Test ◽  
Standard Drug ◽  
In Vivo Test

The objective of this study was to determine the phytoconstituents present in successive extracts and the antidepressant activity of methanolic extract of the spadix of Colocasia affinis. The preliminary phytochemical investigation indicated the presence of fats and fixed oils, steroids and triterpenoids in petroleum ether extract, steroids and triterpenoids in chloroform extract, flavonoids and tannins in methanol extract and carbohydrates in the aqueous extract. An in vivo test for antidepressant activity was performed on the methanolic extract of the plant at two different doses using force swim test and tail suspension test on an experimental animal model (rat). Imipramine was used as a standard drug for the study. The methanolic extract at 200 and 400 mg/kg, produced significant reduction (p<0.001) in the immobility period when compared with that of control group animals in both the tests. Thus, the result indicates that the methanolic extract exhibited significantly good antidepressant activity. Further investigation may be needed to understand the actual mechanism of action.

scholarly journals Pharmacological In vivo test to evaluate the antidepressant activity of polyherbal formulation

2019 ◽  
Vol 7 (1) ◽  
pp. 63-73
Author(s):  
Rinki Kumar ◽  
K. Ilango ◽  
G.P.I. Singh ◽  
G.P. Dubey
Keyword(s):  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Control Group ◽  
Potential Candidate ◽  
Standard Drug ◽  
In Vivo Test ◽  
Polyherbal Formulation ◽  
Immobility Time ◽  
Model Control

The antidepressant effects of the polyherbal formulation (PF) (contain four extracts of medicinal plants namely: Nyctanthes arbortristis, Hippophae salcifolia, Ocimum tenuiflorum and Withania somnifera ) was examined by evaluating the extent of reduction of behavioural alterations and neurotransmitter in the rats stressed by forced swim test (FST). In the present study, compared with the model control group (FST), the altered behavioural parameters were attenuated significantly (P < 0.05) in the group treated with the PF (100, 200 and 400 mg•kg−1), comparable with the standard drug treated group, Sertraline (10mg•kg−1). The PF and Sertraline significantly (P < 0.05) increased the level of the neurotransmitter such as serotonin, dopamine, acetylcholine and noradrenalin whereas decreased the level of monoamine oxidase along with oxidant in the brain of the stressed rats. PF and Sertraline were also involved in the reduced oxidant and generated antioxidant in the stressed rats. The results indicated that polyherbal formulation exhibited significant antidepressant activity, as indicated by its ability to decrease force swim stress, induced immobility time in rats as well as restoring the biogenic amines to normal level that were altered by the swim induced stress in whole rat brain. Therefore, PF can be a potential candidate for treatment of depression as well as a potent antidepressant. However, further studies are required to substantiate the same.

scholarly journals Antidepressant Potential of Lotus corniculatus L. subsp. corniculatus: An Ethnobotany Based Approach

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1299
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Haroon Khan ◽  
Eduardo Sobarzo-Sánchez ◽  
Esra Küpeli Akkol
Keyword(s):  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Lotus Corniculatus ◽  
Antidepressant Effect ◽  
In Vivo Test ◽  
Aerial Parts ◽  
Test Models ◽  
Bioassay Guided Fractionation

As a Turkish traditional medicinal plant, aerial parts of Lotus corniculatus L. subsp. corniculatus (Fabaceae) are used as a painkiller, antihemoroidal, diuretic and sedative. In this study, the antidepressant potential of the plant has been attempted to clarify. Extracts with water, n-Hexane, ethyl acetate, and methanol were prepared respectively from the aerial parts. Antidepressant activity of the extracts were researched by using three different in vivo test models namely a tail suspension test, antagonism of tetrabenazine-induced hypothermia, ptosis, and suppression of locomotor activity and forced swimming test on male BALB/c mice and in vitro monoamine oxidase (MAO)-A and B inhibition assays. The results were evaluated through comparing with control and reference groups, and then active compounds of the active extract have been determined. Bioassay-guided fractionation of active fraction led to the isolation of three compounds and structures of the compounds were elucidated by spectroscopic methods. The data of this study demonstrate that the MeOH extract of the aerial parts of the plant showed remarkable in vivo antidepressant effect and the isolated compounds medicarpin-3-O-glucoside, gossypetin-3-O-glucoside and naringenin-7-O-glucoside (prunin) from the active sub-fractions could be responsible for the activity. Further mechanistic and toxicity studies are planned to develop new antidepressant-acting drugs.

Pyrrolopyrazoles: Synthesis, Evaluation and Pharmacological Screening as Antidepressant Agents

2019 ◽  
Vol 15 (8) ◽  
pp. 911-922 ◽  
Author(s):  
Samar S. Fatahala ◽  
Shahira Nofal ◽  
Eman Mahmoud ◽  
Rania H. Abd El-hameed
Keyword(s):  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Positive Control ◽  
Biologically Active ◽  
Control Group ◽  
Standard Drug ◽  
Behavioral Techniques ◽  
Special Concern ◽  
Pharmacological Screening ◽  
The Brain

Background: Pyrroles and fused pyrroles are of great interest as biologically active compounds, among these activities; antidepressant activity is of special concern. Objective: Synthesis of a series of pyrrolopyrazoles and their pyrimidine derivatives and their characterization using spectral data to be monitored for antidepressant activity using behavioral techniques. Methods: A control group was administered the vehicle i.p., positive control group received fluoxetine as standard and all other groups were administered the tested compounds. The groups were subjected to tail suspension test (TST) to determine the antidepressant activity compared with fluoxetine as a standard drug. The compounds exhibiting antidepressant activity were then used to analyze changes in serotonin (5HT) level in the brain of albino mice. Results: TST results showed that both pyrazoles and pyrazolopyrimidines derivatives exhibit promising anti-depressant activity. Conclusion: Compounds [pyrazoles & pyrazlopyrimidines] showed promising antidepressant activity possibly mediated by the increased levels of 5HT.

Effect of dialkyl- [2-(1-oxa-3,4,9-triaza-fluoren-2-yl-methoxy)ethyl] amines on biogenic amines: new potential antidepressants

2012 ◽  
Vol 90 (12) ◽  
pp. 1585-1590
Author(s):  
Akena Venkatesham ◽  
J. Venkateshwar Rao ◽  
K. Vijay Kumar ◽  
M. Sarangapani ◽  
Krishna Devarakonda
Keyword(s):  
Biogenic Amines ◽  
Forced Swim Test ◽  
Chronic Administration ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Control Group ◽  
Stress Model ◽  
Chronic Unpredictable Stress ◽  
The Brain

A thorough survey of the literature has revealed that indole derivatives have shown various central nervous system activities. This study aims to evaluate the antidepressant activity of the newly synthesized dialkyl- [2-(1-oxa-3,4,9-triaza-fluoren-2-yl-methoxy)ethyl] amines and their effect on biogenic amines. In this study, the synthesized compounds were assessed by in-vivo antidepressant models, by forced swim test and tail suspension test in mice and effect of synthesized compounds on biogenic amines in brain in a chronic unpredictable stress model. The test compounds have demonstrated significant (P < 0.05) reduced immobility duration in mice when compared with the control group animals. The reduced immobility displayed by mice indicates potential antidepressant activity. In this study, chronic unpredictable stress led to decreased monoamine levels in the cortex and hippocampus regions of the brain. With chronic administration of the investigated compounds there is an increased in monoamines in the brain, in the chronic unpredictable stress model. Decreased levels of monoamines induced by the chronic unpredictable stress induced model of depression, were normalized by treatment with the test compounds, which indicates potential antidepressant activity.

scholarly journals The cytotoxic effect of Synadenium grantii extract against human lung carcinoma A549 cells and its role in improvement of histopathological and biomarkers changes in Benzo(a)pyrene-induced lung cancer in rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 962-971
Author(s):  
Mamdouh Moawad Ali ◽  
Mahmoud Khattab ◽  
Mie Afify Mohamed ◽  
Rania Mohsen Abdelsalam ◽  
Khaled Mahmoud ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Methanolic Extract ◽  
A549 Cells ◽  
Treated Group ◽  
Control Group ◽  
Chemopreventive Agents ◽  
Standard Drug ◽  
Cytotoxicity Activity

Lung cancer is one of the most lethal cancers which is causing up to 3 million deaths annually worldwide. Therefore, management of lung cancer needs searching for new chemopreventive agents. This work was designed to inspect the chemopreventive potential of different extracts prepared from branches and leaves of Synadenium grantii for screening their effects on lung cancer cells (A549), then the most active extract was used for combating lung cancer induced in animal model. The in vitro results showed that, the methanolic extract was the most active extract against A549 cells with a notable cytotoxicity activity (IC50: 4.30±0.44 µg/ml), which was close to the activity of standard drug, doxorubicin (IC50: 3.50±0.40 µg/ml). The results of the in vivo  experiment, revealed that in B(a)P-treated group, aspartate (AST) and alanine (ALT) transaminase activities as well as the levels of urea, creatinine, alpha-fetoprotein (AFP) and Phosphotylinosital 3 Kinase (PI3K) were significantly increased comparing to control group. However, treatment with S. grantii  ameliorated the increase in these parameters in both after- and before-treatment groups comparing with B(a)P-treated group. This improvement in biochemical results were also supported by improving in morphological and histopathological injuries induced by B(a)P, which indicated that methanolic extract of S. grantii  has a chemoprevention effect on lung cancer.

scholarly journals EXPERIMENTAL EVALUATION OF ANTIDEPRESSANT AND ANTIANXIETY ACTIVITIES OF AQUEOUS LEAF EXTRACTS OF SENNA ALATA (L.) ROXB. USING IN VITRO ANIMAL MODELS

2016 ◽  
Vol 8 (4) ◽  
pp. 60
Author(s):  
Archana Pamulaparthi ◽  
Vamshi Ramana Prathap ◽  
Mahitha Banala ◽  
Rama Swamy Nanna
Keyword(s):  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Control Group ◽  
Test Drug ◽  
Leaf Extracts ◽  
Standard Drug ◽  
The Mean ◽  
Senna Alata

Objective: In the present investigation antidepressant and antianxiety activities of aqueous leaf extracts of Senna alata (200 mg/Kg) were carried out to establish the species as a potent natural antidepressant and anxiolytic drug.Methods: Antidepressant activity was carried out using forced swim test and tail suspension test. In both these tests, the animals were subjected to external stress that results in alteration of the behavior of animal due to fear.The antianxiety activity of aqueous leaf extracts of S. alata has been studied by the elevated plus-maze test in rats. The mean number of entries and the time spent in the open arm after 45 min of the administration of test drug was noted to determine the antianxiety effect of the test drug.Results: For antidepressant activity administration of test drug (200 mg/Kg) showed a significant decrease in the time spent by the animal in state of depression in both the assays which clearly indicates that the aqueous leaf extracts of S. alata exhibited a strong antidepressant activity similar to that of the control drug (Imipramine).In the antianxiety activity, administration of aqueous leaf extract (200 mg/Kg) of S. alata significantly increased the mean number of entries (2.25±0.98) in the open arm and the time spent in open arm (2.23±0.04) compared to the control group. The activity of the extract was slightly greater than standard drug Diazepam.Conclusion: From the above-presented results, it can be concluded that administration of Senna alata aqueous leaf extracts (200 mg/Kg) showed a considerable decrease in both antidepressant and antianxiety activities in all the test animals and can be used in the replacement of commercially available synthetic drugs in the near future.

scholarly journals Synthesis and Evaluation of Antidepressant Activities of 5-Aryl-4,5-dihydrotetrazolo [1,5-a]thieno[2,3-e]pyridine Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1857 ◽  
Author(s):  
Shiben Wang ◽  
Hui Liu ◽  
Xuekun Wang ◽  
Kang Lei ◽  
Guangyong Li ◽  
...  
Keyword(s):  
Biological Activities ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Homology Model ◽  
Docking Studies ◽  
Control Group ◽  
Pyridine Derivatives ◽  
Immobility Time

In this study, we synthetized a series of 5-aryl-4,5-dihydrotetrazolo[1,5-a]thieno[2,3-e]pyridine derivatives containing tetrazole and other heterocycle substituents, i.e., triazole, methyltriazole, and triazolone. The forced swim test (FST) and tail suspension test (TST) were used to evaluate the antidepressant activity of the target compounds. The compound 5-[4-(trifluoromethyl)phenyl]-4,5-dihydrotetrazolo[1,5-a]thieno[2,3-e]pyridine (4i) showed the highest antidepressant activity, with a reduced immobility time of 55.33% when compared with the control group. Using an open-field test, compound 4i was shown to not affect spontaneous activity of mice. The determination of in vivo 5-hydroxytryptamine (5-HT) concentration showed that compound 4i may have an effect in the mouse brain. The biological activities of all synthetized compounds were verified by molecular docking studies. Compound 4i showed significant interactions with residues of the 5-HT1A receptor homology model.

scholarly journals Design, Synthesis, and In Vivo and In Silico Evaluation of Coumarin Derivatives with Potential Antidepressant Effects

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5556
Author(s):  
Xuekun Wang ◽  
Hao Zhou ◽  
Xinyu Wang ◽  
Kang Lei ◽  
Shiben Wang
Keyword(s):  
Molecular Docking ◽  
High Resolution Mass Spectrometry ◽  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Homology Model ◽  
Coumarin Derivatives ◽  
Monitoring Methods ◽  
Discovery Studio

In this study, a series of coumarin derivatives were designed and synthesized, their structures were characterized using nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) testing methods. In the pharmacological experiment, two behavior-monitoring methods, the forced swim test (FST) and the tail suspension test (TST), were used to determine the antidepressant activity of coumarin derivatives. Compounds that showed potential activity were analyzed for their effects on 5-hydroxytryptamine (5-HT) levels in the brains of mice. Molecular docking experiments to simulate the possible interaction of these compounds with the 5-HT1A receptor was also be predicted. The results of the pharmacological experiments showed that most coumarin derivatives exhibited significant antidepressant activity. Among these compounds, 7-(2-(4-(4-fluorobenzyl)piperazin-1-yl)-2-oxoethoxy)-2H-chromen-2-one (6i) showed the highest antidepressant activity. The results of the measurement of 5-HT levels in the brains of mice indicate that the antidepressant activity of coumarin derivatives may be mediated by elevated 5-HT levels. The results of molecular docking demonstrated that compound 6i had a significant interaction with amino acids around the active site of the 5-HT1A receptor in the homology model. The physicochemical and pharmacokinetic properties of the target compounds were also predicted using Discovery Studio (DS) 2020 and Chemdraw 14.0.

scholarly journals Evaluation of antidiabetic activity of fruit of Coriandrum sativum. Linn methanolic extract in Streptozocin induced diabetic wistar Albino rats

2017 ◽  
Vol 7 (1) ◽  
pp. 121
Author(s):  
Ahmed S. K. ◽  
Chakrapani Cheekavolu ◽  
Sampath D. ◽  
Sunil M.
Keyword(s):  
Research Study ◽  
Diabetes Management ◽  
Methanolic Extract ◽  
Coriandrum Sativum ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Citrate Buffer ◽  
Standard Drug ◽  
Wistar Albino Rats

Background: Diabetes prevalence is estimated to increase annually. Numerous people use traditional medicine, such as India also considered as the diabetic capital in the world. Diabetes is a metabolic disorder characterized by disturbances in lipid, carbohydrate and protein metabolism. The present study to evaluate the antidiabetic potential of coriandrum sativum. linn fruits methanolic extract in streptozocin induced diabetic wistar albino rats model.Methods: Diabetes induction in wistar albino rats by administration of streptozocin (50mg/kg, i.p.) in citrate buffer. 30 wistar albino rats were divided into 5 groups (A, B, C, D, E). Group A: served as normal control, whereas Group B: diabetic control, Group C, D methanolic coriandrum sativum Linn. fruits extract (CSFME) at a dose of 100, 200mg/kg orally, Group E was given standard drug Glibenclamide (0.5mg/kg) orally. All groups are administered for the period of 14 consecutive days and blood sugar levels was measured at regular intervals up to end of the study.Results: This present research study confirms that the test drug compound CSFME has sustained oral hypoglycaemic activity and statistically significant (p ≤0.05) and which is comparable with standard drug Glibenclamide.Conclusions: This research study confirms that the CSFME has antidiabetic activity against streptozocin induced wistar diabetic albino rats. It could be a novel antidiabetic agent and also a dietary adjunct in the type 2 diabetes management and its complication. Further studies are necessary required to confirm the antidiabetic activity of individual phytochemical compounds of Coriandrum sativum.

scholarly journals STREPTOZOTOCIN-INDUCED OXIDATIVE STRESS IN DIABETIC RATS - A DEFENSIVE EFFECT OF PSYDRAX DICOCCOS

2018 ◽  
Vol 11 (11) ◽  
pp. 378
Author(s):  
Ravi Kumar V ◽  
Sailaja Rao P
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Blood Glucose ◽  
Methanolic Extract ◽  
Antioxidant Effect ◽  
Control Group ◽  
Standard Drug ◽  
Antihyperglycemic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective: The present study was aimed to evaluate the antihyperglycemic activity and in vivo antioxidant effect of methanolic extract of whole plant of Psydrax dicoccos (MEPD) belonging to the family Rubiaceae.Methods: MEPD was prepared by Soxhlet extraction. Wistar rats weighing (180–200 g) were divided into six groups (n=6), with three doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg of extract. Metformin was used as a standard drug. Diabetes was induced by streptozotocin (STZ) (40–50 mg/kg, i.p) in control group. The animals were treated with different doses of extracts for 21 days, and on the 22nd day, the blood glucose levels along with antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and lipid peroxidase (LPO) were determined.Results: The phytochemical screening of the extract showed the presence of carbohydrates, phenolics, flavonoids, glycosides, and tannins. The methanolic extract of MEPD at the dose of 200 mg/kg body weight showed a significant reduction in blood glucose levels (**p<0.001) with the value of 151.2 mg/dl on the 22nd day at 8 h. A promising antioxidant effect was also evident from the determination of antioxidant enzymes such as SOD, CAT, and LPO.Conclusion: The P. dicoccos extract revealed a potential effect of antihyperglycemic activity and combating nature on oxidative stress induced by STZ.

Sign in / Sign up

Export Citation Format

Share Document