scholarly journals GEOGRAPHYCAL EFFECT ON THE CYTOTOXIC ACTIVITY OF Annona muricata L. LEAVES ETHANOL EXTRACT AGAINST MCF-7 CANCER CELL

2019 ◽  
Vol 8 (2) ◽  
pp. 12-19
Author(s):  
Usep Suhendar
Keyword(s):  
Cytotoxic Activity ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Chemical Constituent ◽  
Biochemical Properties ◽  
Annona Muricata ◽  
Geographical Regions

Many studies have shown the anti-cancer activities of the chemical compounds extracted from the leaves of Annona muricata or soursop plant. Cianjur and Sukabumi are quite large soursop producing area in Indonesia. This study was carried out to determine the difference of cytotoxic activity of soursop leaves ethanolic extract which were harvested from three different areas of Cianjur (I, II, III) and Sukabumi (I, II, III). The Soursop leaves were macerated with 70% ethanol using microwave assisted extraction (MAE) method. The extract was tested in vitro on breast cancer cell line MCF7 and its constituent was identified using GC-MS apparatus. The results showed that the highest cytotoxic activity with IC50 values of 9.12 µg ml-1 was determined on the extract of soursop leaves harvested from Cianjur III area. Qualitative identification of chemical constituent shows that the soursop leaves contain alkaloid, flavonoid, triterpenoid, tannin and saponin compounds. No steroid compound was detected in the extract. It can be concluded that the geographical regions affected the biochemical properties of soursop leaves.

scholarly journals GEOGRAPHYCAL EFFECT ON THE CYTOTOXIC ACTIVITY OF Annona muricata L. LEAVES ETHANOL EXTRACT AGAINST MCF-7 CANCER CELL

2019 ◽  
Vol 8 (2) ◽  
pp. 71-78
Author(s):  
Usep Suhendar
Keyword(s):  
Cytotoxic Activity ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Chemical Constituent ◽  
Biochemical Properties ◽  
Annona Muricata ◽  
Geographical Regions

Many studies have shown the anti-cancer activities of the chemical compounds extracted from the leaves of Annona muricata or soursop plant. Cianjur and Sukabumi are quite large soursop producing area in Indonesia. This study was carried out to determine the difference of cytotoxic activity of soursop leaves ethanolic extract which were harvested from three different areas of Cianjur (I, II, III) and Sukabumi (I, II, III). The Soursop leaves were macerated with 70% ethanol using microwave assisted extraction (MAE) method. The extract was tested in vitro on breast cancer cell line MCF7 and its constituent was identified using GC-MS apparatus. The results showed that the highest cytotoxic activity with IC50 values of 9.12 µg ml-1 was determined on the extract of soursop leaves harvested from Cianjur III area. Qualitative identification of chemical constituent shows that the soursop leaves contain alkaloid, flavonoid, triterpenoid, tannin and saponin compounds. No steroid compound was detected in the extract. It can be concluded that the geographical regions affected the biochemical properties of soursop leaves.

scholarly journals The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Soilia Fertilita ◽  
Willy Sandhika ◽  
Desak Gede Agung Suprabawati
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
T47d Cell ◽  
Annona Muricata ◽  
T47d Breast Cancer Cell ◽  
Viability Percentage

Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.

In Vitro Tracing of Cytotoxic Compounds in Jarak Cina Stem Bark (Jatropha Multifida Linn.)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Sista Werdyani ◽  
Annisa Fitria ◽  
Sari Rakhmawati
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Jatropha Multifida ◽  
Mcf 7

Cancer remains one of the diseases with increasing number of sufferers, but research on compounds that act as anti-cancer is also ongoing. Terpenoids have been known as a compound that can inhibit the proliferation of cancer cells. One of the medical plants that produce terpenoids is Jarak cina (Jatropha multifida Linn.). Therefore, the possibility of Jarak cina (Jatropha multifida Linn.) to have an cytotoxic activity on cancer cell proliferation is reasonably high. This study was conducted to determine the cytotoxic activity of Jarak cina (Jatropha multifida Linn.) bark extracts against cancer cell MCF-7. Jarak cina bark was extracted using the multilevel soxhlet extraction method with n-hexane, ethyl acetate, and ethanol as the solvents. All the three extracts were then tested against MCF-7 cancer cells using MTT (3-(4,5-dimethylthiazol-2-yl) - 2,5-diphenyltetrazolium bromide) method. Data analysis was performed for IC50 (ppm) parameter. The results showed that the IC50 of n-hexane extract was 313.21 ppm, while the ethyl acetate extract reached 258.38 ppm of IC50, and the IC50 of ethanol extract was 418.51 ppm. The highest potential of cytotoxicity was found in the ethyl acetate extract, so further testing would be required to optimize the proliferation inhibitory activity.

scholarly journals Antiproliferative activity and caspase enhancement properties of Annona muricata leaves extract against colorectal cancer cells

2016 ◽  
Vol 25 (3) ◽  
pp. 136-42
Author(s):  
Lili Indrawati ◽  
Purwantyastuti Ascobat ◽  
Budiman Bela ◽  
Murdani Abdullah ◽  
Ingrid S. Surono ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Water Extract ◽  
Cancer Cell Line ◽  
Colorectal Cancer Cell ◽  
Colorectal Cancer Cell Line ◽  
Annona Muricata ◽  
Caspase 9

Background: The prevalence of colorectal cancer is rising in Asia including Indonesia. Annona muricata tea leaves, that is traditionally used for maintaining health, and lately being used by cancer patients. The objectives of this study is to investigate its effects in human colorectal cancer cell in vitro and ex vivo.Methods: Thirty patients with colorectal cancer (CRC) were enrolled in a randomized double-blind placebo-controlled trial. They were equally divided into two groups: those treated with 300 mg A. muricata leaf extract and placebo daily for 8 weeks. Serum from supplemented CRC patients of both groups was compared for caspase 9 and caspase 8 enhancement activity. Antiproliferative effect of water extract of A. muricata leaves and its fractions were evaluated against colorectal cancer cell line (DLD-1 and COLO 205) compared with 5-fluorouracil and placebo, the dose range was 62.5-2,000 µg/mL. Method used was 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Data were analyzed by Mann-Whitney U test. The p value was set at 0.05.Results: Ethanol-soluble fraction of A. muricata leaves extract water extract (ESFAM) leaves extract had cytotoxicity effects on DLD-1 as well as COLO 205 cell line, as shown by the lower IC50 compared to 5-fluorouracil and placebo, 20.59 μg/mL and 654.9μg/mL, respectively. Serum of subjects supplemented with extract significantly induced caspase 9 (p=0.001) activity of DLD-1 colorectal cancer cell line, but not for caspase 8 activity (p=0.372).Conclusion: The study's results suggest the cytotoxicity potential of  A. muricata  leaves extract  in in vitro and ex vivo studies.

scholarly journals IN VITRO CYTOTOXIC ACTIVITY OF SECOISOLARICIRESINOL DIGLUCOSIDE ON HT-29, PA-1 CELL LINES, AND α-AMYLASE INHIBITORY ACTIVITY

2019 ◽  
pp. 168-173
Author(s):  
GUNABHUSHANA DADDALA ◽  
SWAROOPARANI A
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Inhibitory Activity ◽  
Ovarian Cancer Cell Line ◽  
Cancer Cell Line ◽  
Secoisolariciresinol Diglucoside ◽  
Human Colon Cancer ◽  
Ht 29

Objective: The present study was conducted to evaluate the in vitro cytotoxic activity and α- amylase inhibitory activity of secoisolariciresinol diglucoside (SDG). Methods: The cytotoxic activity was conducted on HT-29 (human colon cancer cell line) and PA-1 (human ovarian cancer cell line) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the α- amylase inhibitory activity using acarbose as a standard. Both the tests were evaluated at different concentrations, 3.125–100 μg/ml and 50–2000 μg correspondingly and the concentration required for a 50% inhibition of viability (IC50) was determined graphically. The effect of the samples on the proliferation of HT-29 and PA-1 was expressed as the percentage cell viability. Results: SDG exhibited a considerable dose- and time-dependent inhibition on both HT-29 and PA-1 and also observed a concentration-dependent α-amylase inhibitory activity that leads in reduction of starch hydrolysis and hence eventually to lowered glucose levels. Conclusion: The present in vitro study concluded that SDG can be a potent anticancer and moderate hyperglycemic component.

scholarly journals Synthesis, Characterization, In Vitro Anticancer Potentiality, and Antimicrobial Activities of Novel Peptide–Glycyrrhetinic-Acid-Based Derivatives

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4573
Author(s):  
Gaber O. Moustafa ◽  
Ahmed Shalaby ◽  
Ahmed M. Naglah ◽  
Marwa M. Mounier ◽  
Heba El-Sayed ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Micrococcus Luteus ◽  
Normal Skin ◽  
Cancer Cell Line ◽  
Glycyrrhetinic Acid ◽  
Pentacyclic Triterpenoids ◽  
Vitro Effect

Glycyrrhetinic acid (GA) is one of many interesting pentacyclic triterpenoids showing significant anticancer activity by triggering apoptosis in tumor cell lines. This study deals with the design and synthesis of new glycyrrhetinic acid (GA)–amino acid peptides and peptide ester derivatives. The structures of the new derivatives were established through various spectral and microanalytical data. The novel compounds were screened for their in vitro cytotoxic activity. The evaluation results showed that the new peptides produced promising cytotoxic activity against the human breast MCF-7 cancer cell line while comparing to doxorubicin. On the other hand, only compounds 3, 5, and 7 produced potent activity against human colon HCT-116 cancer cell line. The human liver cancer (HepG-2) cell line represented a higher sensitivity to peptide 7 (IC50; 3.30 μg/mL), while it appeared insensitive to the rest of the tested peptides. Furthermore, compounds 1, 3, and 5 exhibited a promising safety profile against human normal skin fibroblasts cell line BJ-1. In order to investigate the mode of action, compound 5 was selected as a representative example to study its in vitro effect against the apoptotic parameters and Bax/BCL-2/p53/caspase-7/caspase-3/tubulin, and DNA fragmentation to investigate beta (TUBb). Additionally, all the new analogues were subjected to antimicrobial assay against a panel of Gram-positive and Gram-negative bacteria and the yeast candida Albicans. All the tested GA analogues 1–8 exhibited more antibacterial effect against Micrococcus Luteus than gentamicin, but they exhibited moderate antimicrobial activity against the tested bacterial and yeast strains. Molecular docking studies were also simulated for compound 5 to give better rationalization and put insight to the features of its structure.

scholarly journals Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study

2015 ◽  
Vol 16 (9) ◽  
pp. 709-714 ◽  
Author(s):  
Prachi Shivpuje ◽  
Renuka Ammanangi ◽  
Kishore Bhat ◽  
Sandeep Katti
Keyword(s):  
Oral Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Aqueous Extract ◽  
Cancer Cell Line ◽  
Ocimum Sanctum ◽  
Oral Cancer Cell ◽  
Oral Cancer Cell Line

ABSTRACT Background Cancer till today remains the leading cause of death in both developed and developing countries. Plants have been beacon of therapeutic sources for curing diseases from times immemorial. Hence, the present study aimed at evaluating the antiproliferative activity of extract of Ocimum sanctum leaves on oral cancer cell line. Objectives: • To evaluate the antiproliferative effect and to analyze dose dependent cytotoxic activity of aqueous extract of O. sanctum leaves on KB mouth cell line. • To compare the effectiveness among different variety of O. sanctum. Materials and methods KB cells (Mouth Epidermal Carcinoma Cells) were used for the present study. Aqueous and dry extract of O. sanctum with both dark (Krishna Tulsi) and light (Rama Tulsi) leaves were prepared in the institution. The antiproliferative and cytotoxic activity on KB cell line was evaluated by MTT assay. Statistical analysis with Mann-Whitney U-test and Wilcoxon matched pairs test was carried out. Results The aqueous extract of O. sanctum of both the leaves exhibited significant cytotoxic effect against oral cancer cell line. Conclusion Aqueous extract of O. sanctum leaves was effective as an antiproliferative agent which caused apoptosis in oral cancer cell line. Clinical significance Ocimum sanctum herb which is abundantly grown in India can be used for its anticancer properties for treating oral cancer. This will not only be cost-effective but will also have less or no side effects. How to cite this article Shivpuje P, Ammanangi R, Bhat K, Katti S. Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study. J Contemp Dent Pract 2015;16(9):709-714.

scholarly journals In vitro Cytotoxic Activity of Sesamin Isolated from Vismia baccifera var. dealbata Triana & Planch (Guttiferae) Collected from Venezuela

2008 ◽  
Vol 3 (10) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Fabiola Salas ◽  
Janne Rojas ◽  
Antonio Morales ◽  
Maria E. Ramos-Nino ◽  
Nelida G. Colmenares
Keyword(s):  
Lung Cancer ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Cytostatic Activity ◽  
Lung Cancer Cell

Sesamin extracted from Vismia baccifera var. dealbata was demonstrated to have cytostatic activity on the cancer cell lines tested, particularly the lung cancer cell line, with an IC50 of 1 g/L.

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