scholarly journals Antioxidant activities and protective effects of duck embryo peptides against H2 O2-induced oxidative damage in HepG2 cells

2019 ◽  
Vol 98 (12) ◽  
pp. 7118-7128 ◽  
Author(s):  
Ying He ◽  
Lijun Bu ◽  
Huadong Xie ◽  
Guizhao Liang
Keyword(s):  
Oxidative Damage ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Protective Effects ◽  
Duck Embryo

scholarly journals Strawberry and Achenes Hydroalcoholic Extracts and Their Digested Fractions Efficiently Counteract the AAPH-Induced Oxidative Damage in HepG2 Cells

2018 ◽  
Vol 19 (8) ◽  
pp. 2180 ◽  
Author(s):  
María Ariza ◽  
Tamara Forbes-Hernández ◽  
Patricia Reboredo-Rodríguez ◽  
Sadia Afrin ◽  
Massimiliano Gasparrini ◽  
...  
Keyword(s):  
Biological Activity ◽  
Oxidative Damage ◽  
Hepg2 Cells ◽  
In Vitro Digestion ◽  
Protective Effects ◽  
Beneficial Effects ◽  
Positive Effects ◽  
Digestion Process ◽  
Phytochemical Compounds

Strawberry fruits are highly appreciated by consumers worldwide due to their bright red color, typical aroma, and juicy texture. While the biological activity of the complete fruit has been widely studied, the potential beneficial effects of the achenes (commonly named seeds) remain unknown. In addition, when raw fruit and achenes are consumed, the digestion process could alter the release and absorption of their phytochemical compounds, compromising their bioactivity. In the present work, we evaluated the protective effects against oxidative damage of nondigested and digested extracts from strawberry fruit and achenes in human hepatocellular carcinoma (HepG2) cells. For that purpose, cells were treated with different concentration of the extracts prior to incubation with the stressor agent, AAPH (2,2′-azobis(2-amidinopropane) dihydrochloride). Subsequently, intracellular accumulation of reactive oxygen species (ROS) and the percentage of live, dead, and apoptotic cells were determined. Our results demonstrated that all the evaluated fractions were able to counteract the AAPH-induced damage, suggesting that the achenes also present biological activity. The positive effects of both the raw fruit and achenes were maintained after the in vitro digestion process.

Protective effects of oligomeric and polymeric procyanidin fractions from defatted grape seeds on tert-butyl hydroperoxide-induced oxidative damage in HepG2 cells

2013 ◽  
Vol 137 (1-4) ◽  
pp. 136-141 ◽  
Author(s):  
Younghwa Kim ◽  
Youngmin Choi ◽  
Hyeonmi Ham ◽  
Heon-Sang Jeong ◽  
Junsoo Lee
Keyword(s):  
Oxidative Damage ◽  
Hepg2 Cells ◽  
Protective Effects ◽  
Grape Seeds ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide

Protective effects of green tea polyphenols on human HepG2 cells against oxidative damage of fenofibrate

2003 ◽  
Vol 35 (9) ◽  
pp. 1121-1128 ◽  
Author(s):  
Hong-li Jiao ◽  
Ping Ye ◽  
Bao-lu Zhao
Keyword(s):  
Oxidative Damage ◽  
Green Tea ◽  
Hepg2 Cells ◽  
Tea Polyphenols ◽  
Green Tea Polyphenols ◽  
Protective Effects

scholarly journals Gentiopicroside Ameliorates Oxidative Stress and Lipid Accumulation through Nuclear Factor Erythroid 2-Related Factor 2 Activation

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Meiyu Jin ◽  
Haihua Feng ◽  
Yue Wang ◽  
Siru Yan ◽  
Bingyu Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Regulatory Element ◽  
Cell Counting ◽  
Related Factor ◽  
Nuclear Factor ◽  
Protective Effects ◽  
Akt Inhibitor

The activation of nuclear factor erythroid 2-related factor 2 (Nrf2) is closely related to the alleviation of nonalcoholic fatty liver disease (NAFLD) by regulating oxidative stress and lipid homeostasis. Gentiopicroside (GPS), an iridoid glycoside found in the Gentianaceae, possesses anti-inflammatory and antioxidant effects. However, the protective effects of GPS on lipid accumulation and oxidative damage have not been investigated thoroughly in free fatty acid- (FFA-) induced HepG2 cells and tyloxapol- (Ty-) induced hyperlipidemia mice. Cell counting kit-8 assays, Oil Red O staining, Western blotting analysis, extraction of nuclear and cytosolic proteins, and biochemical index assay were employed to explore the mechanisms by which GPS exerts a protective effect on FFA-induced HepG2 cells and Ty-induced hyperlipidemia mouse model. This paper demonstrates that GPS could effectively alleviate NAFLD by elevating cell viability, reducing fatty deposition, downregulating TG, and activating nucleus Nrf2 in FFA-induced HepG2 cells. Meanwhile, GPS significantly regulated the activation of phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway, Nrf2 antioxidant pathway, peroxisome proliferator-activated receptor α (PPARα), and GPS-inhibited sterol regulatory element-binding protein-1c (SREBP-1c) expression in FFA-stimulated lipid accumulation of HepG2 cells and Ty-treated mice. Interestingly, we highlight that PI3K/AKT inhibitor (LY294002) markedly increased the expression of Nrf2 antioxidant pathway, PPARα, and downregulated SREBP-1c in FFA-stimulated HepG2 cells. For these reasons, we found that the deletion of Nrf2 could lose the protective effects of GPS on the Nrf2 antioxidant pathway and PPARα activation and SREBP-1c inactivation in FFA-stimulated HepG2 cells and Ty-treated mice. GPS treatment had no effect on abnormal lipogenesis and antioxidant enzymes in Ty-induced Nrf2-/- mice. This work gives a new explanation that GPS may be a useful therapeutic strategy for NAFLD through upregulation of the Nrf2 antioxidant pathway, which can alleviate oxidative damage and lipid accumulation.

Protective effects of Syzygium aromaticum oil (Clove) against acrylamide induced hepatic, renal, and testicular toxicity in rats

2018 ◽  
Vol 6 (1) ◽  
pp. 12 ◽  
Author(s):  
Ashraf Elkomy ◽  
Mohamed Aboubakr ◽  
Samar Ibrahim ◽  
Yasmine Abdelhamid
Keyword(s):  
Oxidative Damage ◽  
Antioxidant Activities ◽  
Protective Role ◽  
Albino Rats ◽  
Syzygium Aromaticum ◽  
Testicular Toxicity ◽  
Protective Effects ◽  
Clove Oil ◽  
Organ Protection ◽  
Testicular Dysfunction

This study aimed to investigate the possible protective role of clove oil against acrylamide induced oxidative damage and impairment of liver, kidney, and testicular functions in albino rats. The apparent oxidative damage was associated with evident hepatic, renal, and testicular dysfunction, which was confirmed in histopathological lesions, and increased serum aspartate aminotransferase and alanine aminotransferase activities. Acrylamide decreased serum total protein and albumin contents; increased urea and creatinine contents. Acrylamide also reduced testosterone concentration. Treatment of acrylamide intoxicated rats with clove oil minimized liver, kidney, and testicular histopathological changes and normalized their functions. Our findings demonstrate that acrylamide is not only associated with hepatotoxicity but also nephrotoxicity and testicular toxicity. Clove oil administration provided substantial organ protection against hepatic, renal, and testicular dysfunction induced by acrylamide, which was possibly mediated through their antioxidant activities.   

scholarly journals Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells

Nutrients ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 151
Author(s):  
Huong Thi Lan Nguyen ◽  
Stefan Kasapis ◽  
Nitin Mantri
Keyword(s):  
Oxidative Stress ◽  
Hepg2 Cells ◽  
Antioxidant Activities ◽  
Biochemical Properties ◽  
Cholesterol Homeostasis ◽  
Protective Effects ◽  
Cholesterol Reduction ◽  
Phenolic Contents ◽  
Biomarkers Of Oxidative Stress ◽  
Newtonian Behavior

Manuka honey and newly developed honeys (arjuna, guggul, jiaogulan and olive) were examined for their physicochemical, biochemical properties and effects on oxidative stress and cholesterol homeostasis in fatty acid-induced HepG2 cells. The honeys exhibited standard moisture content (<20%), electrical conductivity (<0.8 mS/cm), acidic pH, and monosaccharides (>60%), except olive honey (<60% total monosaccharides). They all expressed non-Newtonian behavior and 05 typical regions of the FTIR spectra as those of natural ones. Guggul and arjuna, manuka honeys showed the highest phenolic contents, correlating with their significant antioxidant activities. Arjuna, guggul and manuka honeys demonstrated the agreement of total cholesterol reduction and the transcriptional levels of AMPK, SREBP2, HCMGR, LDLR, LXRα. Jiaogulan honey showed the least antioxidant content and activity, but it was the most cytotoxic. Both jiaogulan and olive honeys modulated the tested gene in the pattern that should lead to a lower TC content, but this reduction did not occur after 24 h. All 2% concentrations of tested honeys elicited a clearer effect on NQO1 gene expression. In conclusion, the new honeys complied with international norms for natural honeys and we provide partial evidence for the protective effects of manuka, arjuna and guggul honeys amongst the tested ones on key biomarkers of oxidative stress and cholesterol homeostasis, pending further studies to better understand their modes of action.

scholarly journals Protective effects of the extracts ofBarringtonia racemosashoots against oxidative damage in HepG2 cells

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1628 ◽  
Author(s):  
Kin Weng Kong ◽  
Sarni Mat-Junit ◽  
Norhaniza Aminudin ◽  
Fouad Abdulrahman Hassan ◽  
Amin Ismail ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Hepg2 Cells ◽  
Protocatechuic Acid ◽  
Polyphenolic Compounds ◽  
Oxygen Species ◽  
Protective Effects ◽  
Frap Assay ◽  
Cell Viability Assay ◽  
Tropical Plant ◽  
Viability Assay

Barringtonia racemosais a tropical plant with medicinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots to protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 µg/ml and 500 µg/ml, respectively. BLE and BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots ofB. racemosacan be an alternative bioactive ingredient in the prevention of oxidative damage.

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