scholarly journals Carbon-Coated Magnetic Nanoparticle Dedicated to MRI/Photoacoustic Imaging of Tumor in Living Mice

2021 ◽  
Vol 9 ◽  
Author(s):  
Yujing Li ◽  
Fei Ye ◽  
Shanxiang Zhang ◽  
Wenjun Ni ◽  
Liewei Wen ◽  
...  
Keyword(s):  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Multimodality Imaging ◽  
Morphological Structure ◽  
Quantitative Information ◽  
Dual Modality ◽  
Carbon Coated ◽  
Ferromagnetic Cobalt

Multimodality imaging can reveal complementary anatomic and functional information as they exploit different contrast mechanisms, which has broad clinical applications and promises to improve the accuracy of tumor diagnosis. Accordingly, to attain the particular goal, it is critical to exploit multimodal contrast agents. In the present work, we develop novel cobalt core/carbon shell–based nanoparticles (Cobalt at carbon NPs) with both magnetization and light absorption properties for dual-modality magnetic resonance imaging (MRI) and photoacoustic imaging (PAI). The nanoparticle consists of ferromagnetic cobalt particles coated with carbon for biocompatibility and optical absorption. In addition, the prepared Cobalt at carbon NPs are characterized by transmission electron microscope (TEM), visible–near-infrared spectra, Raman spectrum, and X-ray powder diffraction for structural analysis. Experiments verify that Cobalt at carbon NPs have been successfully constructed and the designed Cobalt at carbon NPs can be detected by both MRI and PAI in vitro and in vivo. Importantly, intravenous injection of Cobalt at carbon NPs into glioblastoma-bearing mice led to accumulation and retention of Cobalt at carbon NPs in the tumors. Using such a multifunctional probe, MRI can screen rapidly to identify potential lesion locations, whereas PAI can provide high-resolution morphological structure and quantitative information of the tumor. The Cobalt at carbon NPs are likely to become a promising candidate for dual-modality MRI/PAI of the tumor.

scholarly journals Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Chuangjia Huang ◽  
Xiaoling Guan ◽  
Hui Lin ◽  
Lu Liang ◽  
Yingling Miao ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Near Infrared ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Good Biocompatibility ◽  
Targeting Ability ◽  
Body Clearance

Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

scholarly journals Assessment of the Theranostic Potential of Gold Nanostars—A Multimodal Imaging and Photothermal Treatment Study

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2112 ◽  
Author(s):  
Antoine D’Hollander ◽  
Greetje Vande Velde ◽  
Hilde Jans ◽  
Bram Vanspauwen ◽  
Elien Vermeersch ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Photoacoustic Imaging ◽  
Tumor Therapy ◽  
Infrared Region ◽  
Tumor Cell Death ◽  
Gold Nanostars ◽  
Theranostic Agents

Gold nanoparticles offer the possibility to combine both imaging and therapy of otherwise difficult to treat tumors. To validate and further improve their potential, we describe the use of gold nanostars that were functionalized with a polyethyleneglycol-maleimide coating for in vitro and in vivo photoacoustic imaging (PAI), computed tomography (CT), as well as photothermal therapy (PTT) of cancer cells and tumor masses, respectively. Nanostar shaped particles show a high absorption coefficient in the near infrared region and have a hydrodynamic size in biological medium around 100 nm, which allows optimal intra-tumoral retention. Using these nanostars for in vitro labeling of tumor cells, high intracellular nanostar concentrations could be achieved, resulting in high PAI and CT contrast and effective PTT. By injecting the nanostars intratumorally, high contrast could be generated in vivo using PAI and CT, which allowed successful multi-modal tumor imaging. PTT was successfully induced, resulting in tumor cell death and subsequent inhibition of tumor growth. Therefore, gold nanostars are versatile theranostic agents for tumor therapy.

scholarly journals Organic nanoparticle-doped microdroplets as dual-modality contrast agents for ultrasound microvascular flow and photoacoustic imaging

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yu Xu ◽  
Guoyun Sun ◽  
Eshu Middha ◽  
Yu-Hang Liu ◽  
Kim Chuan Chan ◽  
...  
Keyword(s):  
Blood Flow ◽  
Nude Mice ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Contrast Effects ◽  
In Vivo Evaluation ◽  
Dual Modality ◽  
In Vivo Experiments

Abstract Tumor blood vessels are chaotic and abundantly distributed, owing to their heterogeneity. Therefore, imaging techniques which reveal abnormalities of tumor vasculature play significant roles in both mechanistic and clinical diagnostic tumor studies. Photoacoustic (PA) imaging uses the intrinsic characteristics of hemoglobin, to acquire tumor hemodynamic information, while ultrasound (US) imaging provides information about tumoral vessel structures and blood flow. To improve the imaging contrast performance, hydrogel-based microdroplets were designed for both US blood flow and PA imaging in this study. The microdroplets served as carriers for PA contrast agent solution in the innermost part while oil and hydrogel formed the inner and outer layers of the droplets. In vitro experiments firstly demonstrated the dual modality contrast effects of the microdroplets on US flow determination and PA imaging. In vivo experiments were then carried out in both healthy nude mice and nude mice with subcutaneous tumor to validate the contrast effects and to monitor the duration of contrast effects in animals. Using the dual-modality microdroplets, we were able to obtain distinct edges of tumor and blood flow mapping of the tumor microvascular with improved sensitivity up to 11.09 dB for PA and 6.69 dB for US flow. Besides, the in vivo evaluation with microdroplets showed US flow enhancement for more than 60 min. Therefore, the microdroplets are able to provide the contrast effects for both US flow and PA in a relative long duration and have potential to be applied in the tumor related diagnoses and studies.

Push–pull type meso-ester substituted BODIPY near-infrared dyes as contrast agents for photoacoustic imaging

2017 ◽  
Vol 15 (21) ◽  
pp. 4531-4535 ◽  
Author(s):  
Yong Ni ◽  
Ravi Kumar Kannadorai ◽  
Sidney W.-K. Yu ◽  
Young-Tae Chang ◽  
Jishan Wu
Keyword(s):  
Contrast Agents ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Nir Absorption ◽  
Photo Stability

Push–pull meso-ester BODIPYs with intense NIR absorption and good photo-stability were used for in vitro and in vivo photoacoustic imaging.

scholarly journals A Semimetal-Like Molybdenum Carbide Quantum Dots Photoacoustic Imaging and Photothermal Agent with High Photothermal Conversion Efficiency

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1776 ◽  
Author(s):  
Wenhao Dai ◽  
Haifeng Dong ◽  
Xueji Zhang
Keyword(s):  
Quantum Dots ◽  
Conversion Efficiency ◽  
Molybdenum Carbide ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Therapeutic Interventions ◽  
High Concentration ◽  
Photothermal Conversion

Theranostic platforms integrating imaging diagnostic and therapeutic interventions into a single nanoplatform have attracted considerable attention for cancer-individualized therapies. However, their uncertain stability, complex pharmacokinetics, and intrinsic toxicology of multiple components hinder their practical application in clinical research. In this paper, stable and high-concentration molybdenum carbide quantum dots (Mo2C QDs) with a diameter of approximately 6 nm and a topographic height of about 1.5 nm were synthesized using a facile sonication-assisted liquid-phase exfoliation approach. The prepared Mo2C QDs exhibited a strong near-infrared (NIR) absorbance with a high molar extinction coefficient of 4.424 Lg−1cm−1 at 808 nm, a high photothermal conversion efficiency of 42.9%, and showed excellent performance on photoacoustic imaging. The Mo2C QDs had high stability and highly biocompatibility, with low cytotoxicity. Under NIR irradiation, a remarkable in vitro and in vivo therapeutic effect was obtained. Such a stable and biocompatible all-in-one theranostic nanoagent generated by facile synthesis that combines promising imaging guidance and effective tumor ablation properties may hold great potential for theranostic nanomedicine.

Second Near-Infrared Photoactivatable Biocompatible Polymer Nanoparticles for Effective in Vitro and in Vivo Cancer Theranostics

Nanoscale ◽  
2021 ◽  
Author(s):  
Fei Wang ◽  
Xiaoju Men ◽  
Haobin Chen ◽  
Feixue Mi ◽  
Mengze Xu ◽  
...  
Keyword(s):  
Photothermal Therapy ◽  
Near Infrared ◽  
Photoacoustic Imaging ◽  
Polymer Nanoparticles ◽  
Tissue Penetration ◽  
Biocompatible Polymer ◽  
Cancer Theranostics ◽  
Maximum Permissible Exposure

Photoacoustic imaging (PAI)-guided photothermal therapy (PTT) has drawn considerable attention due to the deeper tissue penetration and higher maximum permissible exposure. However, current phototheranostic agents are greatly restricted to the...

scholarly journals Fluorescence/photoacoustic imaging-guided nanomaterials for highly efficient cancer theragnostic agent

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vu Hoang Minh Doan ◽  
Van Tu Nguyen ◽  
Sudip Mondal ◽  
Thi Mai Thien Vo ◽  
Cao Duong Ly ◽  
...  
Keyword(s):  
Near Infrared ◽  
High Efficiency ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Non Invasive ◽  
Cell Ablation ◽  
Tumor Tissues

AbstractImaging modalities combined with a multimodal nanocomposite contrast agent hold great potential for significant contributions in the biomedical field. Among modern imaging techniques, photoacoustic (PA) and fluorescence (FL) imaging gained much attention due to their non-invasive feature and the mutually supportive characteristic in terms of spatial resolution, penetration depth, imaging sensitivity, and speed. In this present study, we synthesized IR783 conjugated chitosan–polypyrrole nanocomposites (IR-CS–PPy NCs) as a theragnostic agent used for FL/PA dual-modal imaging. A customized FL and photoacoustic imaging system was constructed to perform required imaging experiments and create high-contrast images. The proposed nanocomposites were confirmed to have great biosafety, essentially a near-infrared (NIR) absorbance property with enhanced photostability. The in vitro photothermal results indicate the high-efficiency MDA-MB-231 breast cancer cell ablation ability of IR-CS–PPy NCs under 808 nm NIR laser irradiation. The in vivo PTT study revealed the complete destruction of the tumor tissues with IR-CS–PPy NCs without further recurrence. The in vitro and in vivo results suggest that the demonstrated nanocomposites, together with the proposed imaging systems could be an effective theragnostic agent for imaging-guided cancer treatment.

scholarly journals Immunocompatibility of a new dual modality contrast agent based on radiolabeled iron-oxide nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria-Argyro Karageorgou ◽  
Dimosthenis Stamopoulos
Keyword(s):  
Complete Blood Count ◽  
Morphological Characteristics ◽  
Mononuclear Phagocyte ◽  
Dual Modality ◽  
Immunodeficient Mice ◽  
Force Microscopy ◽  
Magnetic Resonance Imaging Mri ◽  
Diagnostic Applications

AbstractRadiolabeled magnetic nanoparticles are promising candidates as dual-modality-contrast-agents (DMCA) for diagnostic applications. The immunocompatibility of a new DMCA is a prerequisite for subsequent in vivo applications. Here, a new DMCA, namely Fe3O4 nanoparticles radiolabeled with 68Ga, is subjected to immunocompatibility tests both in vitro and in vivo. The in vitro immunocompatibility of the DMCA relied on incubation with donated human WBCs and PLTs (five healthy individuals). Optical microscopy (OM) and atomic force microscopy (AFM) were employed for the investigation of the morphological characteristics of WBCs and PLTs. A standard hematology analyzer (HA) provided information on complete blood count. The in vivo immunocompatibility of the DMCA was assessed through its biodistribution among the basic organs of the mononuclear phagocyte system in normal and immunodeficient mice (nine in each group). In addition, Magnetic Resonance Imaging (MRI) data were acquired in normal mice (three). The combined OM, AFM and HA in vitro data showed that although the DMCA promoted noticeable activation of WBCs and PLTs, neither degradation nor clustering were observed. The in vivo data showed no difference of the DMCA biodistribution between the normal and immunodeficient mice, while the MRI data prove the efficacy of the particular DMCA when compared to the non-radiolabeled, parent CA. The combined in vitro and in vivo data prove that the particular DMCA is a promising candidate for future in vivo applications.

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