scholarly journals Comparative Transcriptomic Analyses of Antibiotic-Treated and Normally Reared Bactrocera dorsalis Reveals a Possible Gut Self-Immunity Mechanism

2021 ◽  
Vol 9 ◽  
Author(s):  
Jiajin Fu ◽  
Lingyu Zeng ◽  
Linyu Zheng ◽  
Zhenzhen Bai ◽  
Zhihong Li ◽  
...  
Keyword(s):  
Fruit Fly ◽  
Fruit Flies ◽  
Gut Bacteria ◽  
Bactrocera Dorsalis ◽  
Symbiotic Bacteria ◽  
Male Mating ◽  
Gram Positive ◽  
Illumina Hiseq ◽  
Peptidoglycan Recognition Protein ◽  
Host Fruit

Bactrocera dorsalis (Hendel) is a notorious agricultural pest worldwide, and its prevention and control have been widely studied. Bacteria in the midgut of B. dorsalis help improve host insecticide resistance and environmental adaption, regulate growth and development, and affect male mating selection, among other functions. Insects have an effective gut defense system that maintains self-immunity and the balance among microorganisms in the gut, in addition to stabilizing the diversity among the gut symbiotic bacteria. However, the detailed regulatory mechanisms governing the gut bacteria and self-immunity are still unclear in oriental fruit flies. In this study, the diversity of the gut symbiotic bacteria in B. dorsalis was altered by feeding host fruit flies antibiotics, and the function of the gut bacteria was predicted. Then, a database of the intestinal transcriptome of the host fruit fly was established and analyzed using the Illumina HiSeq Platform. The gut bacteria shifted from Gram negative to Gram positive after antibiotic feeding. Antibiotics lead to a reduction in gut bacteria, particularly Gram-positive bacteria, which ultimately reduced the reproduction of the host flies. Ten immunity-related genes that were differentially expressed in the response to intestinal bacterial community changes were selected for qRT-PCR validation. Peptidoglycan-recognition protein SC2 gene (PGRP-SC2) was one of the 10 immunity-related genes analyzed. The differential expression of PGRP-SC2 was the most significant, which confirms that PGRP-SC2 may affect immunity of B. dorsalis toward gut bacteria.

The influence of antibiotics on gut bacteria diversity associated with laboratory-reared Bactrocera dorsalis

2018 ◽  
Vol 109 (4) ◽  
pp. 500-509 ◽  
Author(s):  
Z. Bai ◽  
L. Liu ◽  
M.S. Noman ◽  
L. Zeng ◽  
M. Luo ◽  
...  
Keyword(s):  
Insect Pest ◽  
Fruit Fly ◽  
Gut Bacteria ◽  
Bactrocera Dorsalis ◽  
Oriental Fruit Fly ◽  
Male Adult ◽  
Bacteria Diversity ◽  
Host Fruit ◽  
Wide Range ◽  
Uncultivable Bacteria

AbstractThe oriental fruit fly Bactrocera dorsalis (Hendel) is a destructive insect pest of a wide range of fruit crops. Commensal bacteria play a very important part in the development, reproduction, and fitness of their host fruit fly. Uncovering the function of gut bacteria has become a worldwide quest. Using antibiotics to remove gut bacteria is a common method to investigate gut bacteria function. In the present study, three types of antibiotics (tetracycline, ampicillin, and streptomycin), each with four different concentrations, were used to test their effect on the gut bacteria diversity of laboratory-reared B. dorsalis. Combined antibiotics can change bacteria diversity, including cultivable and uncultivable bacteria, for both male and female adult flies. Secondary bacteria became the dominant population in female and male adult flies with the decrease in normally predominant bacteria. However, in larvae, only the predominant bacteria decreased, the bacteria diversity did not change a lot, likely because of the short acting time of the antibiotics. The bacteria diversity did not differ among fruit fly treatments with antibiotics of different concentrations. This study showed the dynamic changes of gut bacterial diversity in antibiotics-treated flies, and provides a foundation for research on the function of gut bacteria of the oriental fruit fly.

scholarly journals Host Suitability Index for Polyphagous Tephritid Fruit Flies

2021 ◽  
Author(s):  
Peter A Follett ◽  
Fay E M Haynes ◽  
Bernard C Dominiak
Keyword(s):  
Fruit Fly ◽  
Fruit Production ◽  
General Purpose ◽  
Fruit Flies ◽  
Host Suitability ◽  
Bactrocera Dorsalis ◽  
Infestation Rate ◽  
Suitability Index ◽  
Host Status ◽  
Tephritid Fruit Flies

Abstract Tephritid fruit flies are major economic pests for fruit production and are an impediment to international trade. Different host fruits are known to vary in their suitability for fruit flies to complete their life cycle. Currently, international regulatory standards that define the likely legal host status for tephritid fruit flies categorize fruits as a natural host, a conditional host, or a nonhost. For those fruits that are natural or conditional hosts, infestation rate can vary as a spectrum ranging from highly attractive fruits supporting large numbers of fruit flies to very poor hosts supporting low numbers. Here, we propose a Host Suitability Index (HSI), which divides the host status of natural and conditional hosts into five categories based on the log infestation rate (number of flies per kilogram of fruit) ranging from very poor (<0.1), poor (0.1–1.0), moderately good (1.0–10.0), good (10–100), and very good (>100). Infestation rates may be determined by field sampling or cage infestation studies. We illustrate the concept of this index using 21 papers that examine the host status of fruits in five species of polyphagous fruit flies in the Pacific region: Bactrocera tryoni Froggatt, Bactrocera dorsalis (Hendel), Bactrocera latifrons (Hendel), Zeugodacus cucurbitae (Coquillett), and Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). This general-purpose index may be useful in developing systems approaches that rely on poor host status, for determining surveillance and detection protocols for potential incursions, and to guide the appropriate regulatory response during fruit fly outbreaks.

Non-host status of papaya cultivars to the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), in relation to the degree of fruit ripeness

2016 ◽  
Vol 37 (01) ◽  
pp. 19-29 ◽  
Author(s):  
Domingos Cugala ◽  
João Jone Jordane ◽  
Sunday Ekesi
Keyword(s):  
Fruit Ripening ◽  
Market Access ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Laboratory Evaluation ◽  
Ripening Stage ◽  
Field Cage ◽  
Major Barrier ◽  
Ripening Stages

AbstractPhytosanitary measures are a major barrier to trade in papaya. We assessed the infestation of tephritid fruit flies on different stages of maturity of papaya, to determine its non-host stage of maturity, for market access. Papaya fruits were collected from Kilifi and Embu counties, Kenya from March 2013 to December 2014, to assess the level of infestation by fruit flies according to the degree of fruit ripening. In all locations, no fruit fly infestation was recorded on papaya when fruits were at the 0, 25 and 50% yellow fruit ripening stage.Bactrocera dorsalis(Hendel) was, however, observed attacking fruits when papaya fruits were at 75 and 100% all yellow (fully ripe fruit ripening stage) with infestations of 0.19−0.51B. dorsalis/kg fruit and 0.24−1.24B. dorsalis/kg fruit, respectively, in all locations. Field cage exposure ofB. dorsalisto fruits of five papaya cultivars—‘Papino’, ‘Neo Essence’, ‘Sunrise Solo’, ‘Tainung No. 1’ and ‘Tainung No. 2’ in Manica Province, Mozambique—showed thatB. dorsalisdid not infest fruits at 0, 25 and 50% yellow ripening stages at the densities of 50 and 100 flies per cage. However, at 75% yellow ripening stage, up to 13.1 pupae/kg of fruits was recorded at a density of 150 flies per cage in Tainung No. 1, and infestation ranged from 4.5 to 136 pupae/kg fruits at 100% yellow ripening stage across all the cultivars and infestation densities. Laboratory evaluation of volatiles emanating from freshly crushed papaya pulp of four cultivars: ‘Sunrise Solo’, ‘Red Lady’, ‘Papayi’ and ‘Apoyo’ on egg viability ofB. dorsalisshowed that at 0, 25 and 50% yellow, egg hatchability was inhibited, suggesting that semiochemical compounds present in green tissues of papaya prevent egg development, although this effect was variable across the four cultivars and ripening stages. Export papaya is harvested at less than 40% yellow ripening stage. Our results, therefore, suggest that quarantine treatment for fruits at this ripening stage is inconsequential, asB. dorsalisdoes not infest papaya fruits at this stage; thus, authorities should permit entry of these papaya cultivars of less than 40% yellow ripening stage to quarantine-sensitive markets.

Host range and species diversity of Tephritidae of three plant formations in Western Burkina Faso

2020 ◽  
Vol 110 (6) ◽  
pp. 732-742
Author(s):  
Issaka Zida ◽  
Souleymane Nacro ◽  
Rémy Dabiré ◽  
Laura Moquet ◽  
Hélène Delatte ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Host Range ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
Indigenous Plants ◽  
New Host ◽  
Host Fruit ◽  
Fruit Species ◽  
Wild Fruit ◽  
Western Burkina Faso

AbstractIn Western Burkina Faso, the host range of fruit flies was evaluated in three plant formations between May 2017 and April 2019. Samples of 61 potential hosts were collected and incubated for fruit fly emergence. Twenty-seven hosts including cultivated and wild fruit were identified. Among cultivated fruit species, mango, and guava were the most infested while high infestation incidences were observed in the fruit of the indigenous plants Vitellaria paradoxa, Annona senegalensis, Sarcocephalus latifolius, and Saba senegalensis. Low infestation rates were observed in Anacardium occidentale, Citrus species, Opilia celtidifolia, and Cissus populnea. The highest infestation index (1648.57 flies kg−1) was observed from V. paradoxa. Eleven new host fruit infested with many fruit fly species are reported in Burkina Faso. A total of 18 fruit fly species were reared; Bactrocera dorsalis (42.94%), Ceratitis cosyra (29.93%), and Ceratitis silvestrii (22.33%) dominated those that emerged. Four fruit fly species have been detected for the first time in Burkina Faso. The main suitable fruit hosts are abundant and available from May through August during the rainy season and become rare and have low infestation from November to April during the dry season. This is the first study of its kind in the region. This study shows that the three plant formations had an impact on population dynamics of the three tephritid species of economic importance in Western Burkina Faso. This information should be integrated into the development of a fruit fly pests management strategy.

Effect of fruit and host fly species on the associative learning by Fopius arisanus

2019 ◽  
Vol 109 (05) ◽  
pp. 649-658
Author(s):  
A. Monsia ◽  
G.S.B. Mègnigbèto ◽  
D. Gnanvossou ◽  
M.F. Karlsson
Keyword(s):  
Biological Control ◽  
Associative Learning ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
Pest Species ◽  
Fopius Arisanus ◽  
Host Fruit ◽  
Control Programmes ◽  
Odour Learning ◽  
Ceratitis Cosyra

AbstractParasitoids, released in augmentative biological control programmes, which display a rapid host-location capacity, have a higher likelihood of successfully controlling target pest species. By learning to associate sensory cues to a suitable oviposition site, might parasitoids used as biological control agents, locate hosts more rapidly, and perhaps increase the efficacity of e.g. Tephritidae fruit fly management. We studied associative learning of Fopius arisanus (Hymenoptera: Braconidae) and tested its range of learning in natural and conditional hosts and host fruits, i.e. Bactrocera dorsalis, Zeugodacus cucurbitae, Ceratitis capitata and Ceratitis cosyra (Diptera: Tephritidae) and on fruits (papaya, tomato, banana). Naïve female F. arisanus were compared with experienced wasps, which had been offered infested and non-infested fruit, and been allowed to oviposit. Preferences for olfactory cues from infested fruits were thereafter assessed in a two-choice olfactometer. Naïve and trained parasitoids preference differed in general and non-responders to infested fruits were higher among naïve parasitoids. The trained wasps preferred the fruit infested in the training more than the control fruit, for all combination, except when C. cosyra infested the fruits, hence avoidance behavioural response was observed towards the odour of the infested fruit. Fopius arisanus was capable of behaviourally respond to the learned information, e.g. associative odour learning was achieved, yet limited depending on interaction level, fruit fly and fruit combination. To create F. arisanus preference of an associated odour, it might hence be needed to ensure oviposition in perceived suitable host and host fruit, for the parasitoid learning to become favourable in a biological control setup.

scholarly journals Oviposition Preference of Bactrocera dorsalis Hendel (Diptera: Tephritidae) on Different Fruit in Snake Fruit Orchard

2021 ◽  
Vol 24 (2) ◽  
pp. 224
Author(s):  
Rahmi Fitrah ◽  
Deni Pranowo ◽  
Suputa Suputa
Keyword(s):  
Plant Protection ◽  
Oviposition Preference ◽  
Fruit Fly ◽  
Psidium Guajava ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Trap Crops ◽  
Guava Fruit ◽  
Star Fruit ◽  
Fruit Orchard

The oriental fruit fly Bactocera dorsalis (Hendel) is an important pest of snake fruit (Salacca zalacca) in Sleman District. Due to the high level of damage by the fruit flies, it is necessary to do. The aim of this research was to find out suitable fruit traps, by testing the oviposition preferences of the fruit flies in the orchard to lay eggs on several types of fruit. The research was done in snake fruit orchard located at Sleman Yogyakarta and owned by farmers, while the Lab works were done at the Laboratory of Entomology, Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. All researches were done between April-June 2019. Guava (Psidium guajava), watery rose apples (Syzygium aqueum), starfruit (Averrhoa carambolae), and snake fruit (Salacca zalacca) were used as trap crops. Each fruit, with the same maturity level, was hung 1.5 m above the ground for 4 days with 12 days total trapping at intervals of 3 times. After the test, each fruit was taken and the insects in it were reared in the laboratory. The number of pupae and flies that emerged from each fruit was counted and compared. The results showed that in the orchard 1 as well as  2, of guava fruit produced the highest number of (151 pupae) followed by salak (94 pupae), star fruit (83 pupae), and water guava (2 pupae). The finding of seeds shows that guava fruit is the most suitable host for the fruit flies to be used in the trapping, followed by star fruit and watery guava.

scholarly journals Effectiveness of Selected Parapheromones for Mass Trapping of Mango Fruit Fly, Ceratitis cosyra Walker and Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae) on Mango

2020 ◽  
Vol 36 (1) ◽  
pp. 118-129
Author(s):  
C. Ebi
Keyword(s):  
Mangifera Indica ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Methyl Eugenol ◽  
Bactrocera Dorsalis ◽  
Economic Damage ◽  
Special Focus ◽  
Mass Trapping ◽  
Male Annihilation ◽  
Ceratitis Cosyra

Mango (Mangifera indica L.) is an important fruit crop in Nigeria. It is a source of essential vitamins and is also cultivated for its nutritional, medicinal and industrial uses. Fruit flies infest various commercial fruit crops and cause economic damage. Mass trapping and male annihilation technique (MAT) has been the most useful and common means of controlling fruit flies with special focus on Bactrocera dorsalis Hendel (Diptera: Tephritidae) on mangoes. The study evaluated the effectiveness of four types of parapheromones namely, methyl eugenol (liquid and solid forms), cuelure, terpinyl acetate and trimedlure for mass trapping of fruit flies on Mango. Modified Lynfield traps containing the parapheromones were randomly set on the mango orchards in three replicates in Nigeria Horticultural Research Institute (NIHORT) orchard, Okigwe, Imo state, Southeast Nigeria. This study was conducted during the mango fruiting period of 2019 season, when the density of fruit flies peaked. Effect of parapheromones on mean number of damaged dropped fruits was also evaluated. The mean population of B. dorsalis and Ceratitis cosyra was significantly higher (P>0.05) in liquid methyl eugenol traps when compared to other parapheromones. Bactrocera dorsalis recorded more than 90% of the fruit flies that were trapped especially in the first 3 weeks of trapping. In orchard I, Liquid methyl eugenol significantly (P<0.05) trapped highest number of B. dorsalis (270.20) in week I and similar trends were observed in orchard II. There was steady decline in damaged dropped mango fruits as the study progressed (Fig. 1 and 2). Use of liquid methyl eugenol was most effective in trapping B. dorsalis and C. cosyra, and it can be incorporated in Integrated Pest Management (IPM) programmes for the control of fruit flies.

scholarly journals Pengaruh Penambahan Beberapa Esens Buah pada Perangkap Metil Eugenol terhadap Ketertarikan Lalat Buah Bactrocera dorsalis Kompleks pada Pertanaman Mangga di Desa Pasirmuncang, Majalengka

2018 ◽  
Vol 22 (2) ◽  
pp. 150
Author(s):  
Agus Susanto ◽  
Wahyu Daradjat Natawigena ◽  
Lindung Tri Puspasari ◽  
Neng Inne Nur Atami
Keyword(s):  
Control Method ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Methyl Eugenol ◽  
The Other ◽  
Bactrocera Dorsalis ◽  
West Java ◽  
Male And Female

Fruit fly (Bactrocera dorsalis Complex) is one of the major pests in horticultural commodities in Indonesia. In this present study, a control method of formulating methyl eugenol and the addition of fruit essences was tested to attract B. dorsalis Complex. The objective was to find out the effect of fruit essences addition in the performance of methyl eugenol in attracting fruit flies and to obtain the most effective fruit essences for attracting male and female fruit flies. The experiment was conducted on mango plantation in Pasirmuncang village, Majalengka, West Java from March 2016 until December 2016. Several synthetic fruit essences which were separately added to methyl eugenol in this experiment were mango, citrus, guava, and starfruit essences. The results showed that the addition of fruit essences on methyl eugenol traps had the same effectiveness  or as good as  any single methyl eugenol trap. Moreover, the additions of mango and orange essences  were not effective enough in attracting female fruit flies, although the number of female fruit flies that were caught were more than the other treatments. IntisariLalat buah Bactrocera dorsalis Kompleks merupakan salah satu hama utama pada komoditas hortikultura. Pada penelitian ini, metode pengendalian dengan memformulasikan metil eugenol dan tambahan esens buah diuji untuk menarik B. dorsalis Kompleks. Tujuannya adalah untuk mengetahui efek penambahan esens buah pada kinerja perangkap metil eugenol dalam menarik lalat buah serta untuk mendapatkan esens buah yang efektif untuk menarik lalat buah betina. Penelitian ini dilakukan di perkebunan mangga di desa Pasirmuncang, Majalengka, Jawa Barat dari bulan Maret 2016 hingga bulan Desember 2016. Beberapa jenis esens buah sintetik yang secara terpisah ditambahkan pada metil eugenol pada percobaan ini adalah mangga, jeruk, jambu biji, dan belimbing. Hasil penelitian menunjukkan bahwa penambahan esens buah pada perangkap metil eugenol memiliki keefektifan yang sama baiknya dengan perangkap metil eugenol secara tunggal. Selain itu, penambahan esens mangga dan esens jeruk belum cukup efektif dalam menarik lalat buah betina meskipun jumlah lalat buah betina yang tertangkap lebih banyak dibandingkan dengan perlakuan lainnya.

scholarly journals EFFECT OF COMMUNITY BASED AREA-WIDE FRUIT FLY SUPPRESSION ON BACTROCERA DORSALIS POPULATION IN ORANGE ORCHARDS IN MUHEZA DISTRICT TANZANIA

2016 ◽  
Vol 4 (9) ◽  
pp. 39-44
Author(s):  
ZuberiSingano Seguni
Keyword(s):  
Fruit Fly ◽  
Fruit Flies ◽  
Methyl Eugenol ◽  
Bactrocera Dorsalis ◽  
Large Area ◽  
Community Based ◽  
Simultaneous Application ◽  
Mass Capture ◽  
Crop Area ◽  
Large Crop

A community-based area wide fruit fly suppression exercise involving simultaneous application by farmers of methyl eugenol-mediated mass capture of males of Bactrocera dorsalis fruit fly, crop sanitation by burial of fallen fruits and SUCCESS bait (GF 121)spot application of citrus tree crowns, was undertaken in three villages in Muheza district, Tanga region north eastern Tanzania. Male fruit fly numbers in methyl eugenol traps dropped significantly following application of the treatments from 970 flies per trap per week before application of treatments in July 2011 to 100 and 46 flies six and nine months after treatments at Kwabada village; 200 flies per trap per week before to 34 and one flies after treatment at Kwemsaa village and from 800 flies before to 64 and 50 flies per trap per week during the same period at Mlingano village. However, a fall occurred also in untreated orchards indicating a possible spill-over effect of the treatments due to the large area covered. Moreover, the treated areas might have acted as a sink that drew fruit flies from neighbouring untreated orchards into it. The drastic fall in trapped males may be associated with the effect of the treatments in reducing the number of flies so that fewer were available to fly into the traps. The collective and simultaneous application by farmers of fruit fly suppression over a large crop area is a robust strategy to manage fruit flies in citrus and other fruits orchards such as mango. The results suggest that farmers can successfully implement area-wide fruit fly suppression in smallholder citrus systems.

scholarly journals Diversity and Host Plants of Tephritidae From Two Islands of the Comoro Archipelago (Grande-Comore and Moheli).

2021 ◽  
Author(s):  
KASSIM Bakar ◽  
Turgay ÜSTÜNER
Keyword(s):  
Host Plants ◽  
Prunus Persica ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Bactrocera Dorsalis ◽  
Wild Plants ◽  
Wild Plant ◽  
New Host ◽  
Grande Comore ◽  
Fruit Plants

Abstract This paper summarizes the different host plants and fruit flies present in two islands (Grande-Comore and Mohéli) of the Comoros Archipelago. Different exotic and wild fruit plants were sampled. Eighty plant species, potential hosts, belonging to thirty-four families were collected and incubated for the emergence of fruit flies from December 2019 to September 2020. Twenty-five plant hosts from ten families comprising cultivated and wild fruits have been identified. Fruit fly infestation rates per kilogram of fruit (T.Kg-1 ) varied from plant to plant. Exotic fruit plants, which accounted for more than half of infested plants, including Cucumis melo, Cucurbita pepo, Prunus persica, Coffea arabica and Capsicum frutescens had high infestation rates. For wild plants, the highest infestation rates have been observed in some families including Combretaceae, Cucurbitaceae, Solanaceae and Vitaceae. The highest infestation rate per kilogram of fruit was observed in a wild plant: Cyphostemma lageniflorum. Thirteen new host plants infested by Tephritidae are reported and/or listed for the first time in Comoros. In total, eight species of fruit flies identified. However, the species Bactrocera dorsalis Hendel, 1912 (47.5%) and Dacus bivittatus (Bigot, 1858) (37.6%) were the most representative of the Tephritidae that emerged.

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