Antifreeze Protein Supplementation During the Warming of Vitrified Bovine Ovarian Tissue Can Improve the Ovarian Tissue Quality After Xenotransplantation

The occurrence of ice crystallization during ovarian tissue (OT) cryopreservation causes unavoidable cryodamage, and ice recrystallization during the warming is more detrimental than ice crystallization. Here, we investigated that antifreeze protein (AFP) treatment during the warming procedure can improve the bovine OT quality after xenotransplantation (XT). Bovine OTs (n=120) were evenly assigned to four groups: fresh, vitrified-warmed, vitrified-warmed with 10 mg/mL Leucosporidium ice-binding protein (LeIBP, a type of AFP) (LeIBP-10), and vitrified-warmed with 20 mg/mL LeIBP (LeiBP-20). LeIBPs were added to the first warming solution. Twenty pieces of OTs were assigned to each category. The remaining 10 OTs from each category were assigned to the XT-Fresh control, XT-Vitrified-warmed control, XT-LeIBP-10, and XT-LeIBP-20 groups, respectively, and xenotransplanted to 9-week-old ovariectomized nude mice for one week. LeIBP treatment during the warming step increased morphological follicle normality and decreased apoptotic follicle ratios after vitrification-warming and XT. The XT-vitrified-warmed control group showed significantly reduced microvessel density and increased fibrosis when compared to that of the XT-fresh group. Microvessel density and fibrosis were recovered in both LeIBP treated groups. There was no significant difference between the LeIBP-10 and LeIBP-20 groups in all outcomes. AFP treatment during the warming procedure can prevent OT damage, and improve ovarian follicle morphology and apoptosis in both the vitrified-warmed bovine OT and its graft. After confirmation in a human study, AFPs can potentially be applied to human OT cryopreservation to reduce cryodamage and improve the OT quality.

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JPMA-2019-06-735 Bioevaluation of agro-waste for postprandial glucose and insulin concentration in blood

Journal of the Pakistan Medical Association ◽

10.47391/jpma.735 ◽

2020 ◽

pp. 1-10

Author(s):

Huma Umbreen ◽

Sadia Javed ◽

Razia Noreen ◽

Munazzah Meraj ◽

Admin

Keyword(s):

Human Study ◽

Postprandial Glucose ◽

Apple Pomace ◽

Insulin Concentration ◽

Control Group ◽

Single Subject ◽

White Flour ◽

Significant Difference ◽

Fruit Processing ◽

Mango Peels

Abstract Objective: The objective of the study was to evaluate the effect of apple pomace and mango peels powder supplemented cookies on postprandial glucose and insulin concentration to promote nutraceutical snacks between the meals. Methods: This single subject experimental study was conducted during Feb-Aug 2018 at Nutrition Counseling Center, GCWUF. In this study different cookies with apple pomace and mango peels powder were prepared and the most nutritive acceptable cookies were further used to determine their postprandial effect on glucose and insulin concentrations against the control cookies in female subjects. Thirty females were selected through advertisement having age 25±10 years with normal BMI (19-24.9) while the subjects having obesity, diabetes, hypertension or allergic reaction to any of content were excluded from this study. A written informed consent was obtained from all subjects. The data obtained was analyzed statistically to know the significant difference between the means. Results: The results showed that cookies supplemented with up to 15% of either mango peels or apple pomace powders were acceptable, which were further used for bio-evaluation. The human study showed that overall blood glucose and insulin concentrations were significantly lower in the treatment group specially in group with mango peels enriched cookies (103.53±2.44 mg/dL; 130.48±12.52 mg/dL) as compared to control group receiving white flour based cookies (145.00±1.15 mg/dL; 186.47±26.47 mg/dL). Conclusion: On the basis of the study it can be concluded that fruit processing waste can be used as nutraceutical agent in diet based modules and thus promoting healthy snacking which maintains postprandial

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The Effect of Protein Supplementation and Nutrition Counselling on Protein Catabolic Rates Among Persons with End-Stage Renal Disease on Maintenance Haemodialysis (P12-035-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz035.p12-035-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Patrice Prout ◽

Selby Nichols

Keyword(s):

Nutrition Education ◽

West Indies ◽

Protein Supplementation ◽

Control Group ◽

The West ◽

Maintenance Haemodialysis ◽

Biochemical Variables ◽

Significant Difference ◽

The University ◽

The Impact

Abstract Objectives To compare the impact of protein supplementation versus nutrition education/counselling on protein intakes (indexed by normalized protein catabolic rates (nPCR)) among persons on maintenance haemodialysis. Methods An intervention study design was employed in this investigation. Participating dialysis centers were randomly selected and persons attending those centers were invited to participate in the study. Participants had anthropometry and biochemical variables recorded at baseline and on two occasion 1 month apart during each of the interventions. Persons in the intervention group (IVG) received a supplement containing 36 grams of whey protein daily for 6 weeks in. Following a 2-week washout period, they received personalized weekly nutrition education/counselling for an additional 6 weeks. The control group (NIVG) did not receive any supplementation or nutrition education/counselling. Both groups received received the routine facility care. Participation was voluntary. The study was approved by the Ethics Committee, The University of The West Indies. Results Sixty-eight persons (IVG = 32; NIVG = 36) participated in the study. There was no significant difference in the mean nPCR between IVG and NIVG at baseline (0.84 ± 0.17 vs. 0.84 ± 0.17 mg/kg; P = 0.76). IVG had significantly higher mean nPCR than NIVG during protein supplementation (0.95 ± 0.21 vs. 0.78 ± 0.15 mg/kg; P < 0.001) and nutritional education/counselling (0.87 ± 0.17 vs. 0.79 ± 0.18 mg/kg; P = 0.02 phases of the study. Among IVG, protein supplementation (+Δ 12.8 ± 3.0 mg/kg (±S.E.) but not nutrition education/counselling (+Δ 2.8 ± 2.7 mg/kg; P = 0.32) resulted in a significant increase in mean nPCR compared to baseline.with no significant changes in biochemical variables. Conclusions Among participants nPCR increased significantly with protein supplementation but not nutrition education/counselling without adversely affecting biochemical variables. Funding Sources Grant and Publication Fund, The University of The West Indies.

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Hippocampal size did not differ between epileptic and non-epileptic dogs using volumetric and subjective methods

Acta Veterinaria Hungarica ◽

10.1556/004.2021.00006 ◽

2021 ◽

Author(s):

Borbála A. Lőrincz ◽

Agustina Anson ◽

Stephan Handschuh ◽

Alexander Tichy ◽

Conor Rowan ◽

...

Keyword(s):

Body Weight ◽

Human Study ◽

Positive Association ◽

Brain Structures ◽

Hippocampal Volume ◽

The Body ◽

Control Group ◽

Volumetric Assessment ◽

Significant Difference ◽

Group A

AbstractHippocampal changes in epilepsy may manifest as hippocampal atrophy/sclerosis. A recent human study suggests that the demonstration of hippocampal volume loss is more reliable using quantitative evaluation methods. The aim of the present study was to obtain volumetric data in both epileptic and healthy dogs, to compare hippocampal volumes in both groups, and to compare subjective and volumetric assessment. Volumetric measurements of the hippocampi, lateral ventricles and hemispheria were performed in 31 epileptic and 15 control dogs. There was a positive association between the body weight and the hemispheric volume, as well as between the hemispheric volume and the ipsilateral hippocampal volume. There was no significant correlation between age and the volume of any measured brain structures. There was no statistically significant difference between the hippocampal volumes of the control group and the epileptic group. A statistically significant difference between the two groups for hippocampus/hemispherium ratio or hippocampal asymmetric ratio was not identified. An extrapolated hippocampal volume based on body weight was not possible in this study population.

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Improvement in Ovarian Tissue Quality with Supplementation of Antifreeze Protein during Warming of Vitrified Mouse Ovarian Tissue

Yonsei Medical Journal ◽

10.3349/ymj.2018.59.2.331 ◽

2018 ◽

Vol 59 (2) ◽

pp. 331 ◽

Cited By ~ 2

Author(s):

Hyun Sun Kong ◽

Eun Jung Kim ◽

Hye Won Youm ◽

Seul Ki Kim ◽

Jung Ryeol Lee ◽

...

Keyword(s):

Ovarian Tissue ◽

Antifreeze Protein ◽

Tissue Quality

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Effects of L-Carnitine on follicular reserve and Caspase-3 in transplanted mouse ovarian tissue

Journal of Shahid Sadoughi University of Medical Sciences ◽

10.18502/ssu.v26i12.662 ◽

2019 ◽

Author(s):

Fatemeh Shahi Sadrabadi ◽

Kazem Parivar ◽

Hussein Imani ◽

Abdolhussein Shahverdi

Keyword(s):

Caspase 3 ◽

Sham Group ◽

Ischemia Reperfusion ◽

Ovarian Tissue ◽

Control Group ◽

Ovarian Tissue Transplantation ◽

Study Results ◽

Significant Difference ◽

Follicular Reserve ◽

One Way Anova

Introdution: One of the major challengs in ovarian tissue transplantation is overcomeing ischemia/ reperfusion injuries. During ischemia–reperfusion processes, oxygen free radicals constitute the most important component that induces damage of the grafted tissues. The aim of this study was to investigate the effect of L-Carnitine (LC) as an antioxidant on heterotopic transplantation of mouse ovarian tissue. Methods: In this laboratory experimental study, 5- week old female NMRI mice were divided into four groups: control, transplanted without administration (autograft), sham group (autograft+ saline) and LC group (autograft+ L- carnitine). Left ovarian tissues were transplanted into the Gluteal muscle for 3 weeks. After this time, ovarian tissues from all groups were removed and fixed in formalin for histological studies. Furthermore, rate of Caspase- 3 was assessed by immunohistochemistry test. Lipid peroxidation was assessed by measuring malondialdehyde (MDA). One-way ANOVA and Tudey test was used to analyze the data using the spss 16 software. Significance was defined as P≤0.0. Results: The study results indicated that total follicular count in transplantedwithout administration and sham groups was significantly lower than the control group (p<0.05), but there was no significant difference between the control and LC groups. In addition, the rate of caspase-3 was decreased in the LC group, but no significant difference existed between all groups (p<0.05). A significant reduction in the concentration of MDA was observed in the LC group than that in the other transplanted groups (p<0.05). Conclusion: In this study, LC could improve the ovarian reserve to some extent, but its effect was not significant.

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The Assessment of Angiogenesis by Microvessel Density in Patients With Atrophic/Erosive or Reticular Oral Lichen Planus

ACTA MEDICA IRANICA ◽

10.18502/acta.v59i2.5574 ◽

2021 ◽

Author(s):

Maryam Mardani ◽

Azadeh Andisheh-Tadbir ◽

Mahya Haghparast

Keyword(s):

Lichen Planus ◽

Microvessel Density ◽

Oral Lichen Planus ◽

Precancerous Lesions ◽

Oral Disease ◽

Staining Procedure ◽

Control Group ◽

Significant Difference ◽

The Mean ◽

Oral Lichen

The role of angiogenesis in the development of chronic inflammatory diseases, including oral lichen planus (OLP), is of great concern. The most representative method for the assessment of angiogenesis is the semi-quantification of microvessel density (MVD) using specific markers such as CD105. We aimed to assess the MVD in patients with OLP and find its clinical significance to differentiate the atrophic/erosive forms from reticular ones. This cross-sectional study was conducted on 82 patients with clinically and histopathologically proven cases of OLP, including reticular (n=52) or atrophic/erosive (n=30) lesions. The control group comprised 82 age- and sex-matched subjects without any oral disease. To assess the MVD using CD105, tissue blocks were sliced, and the immunoexpression of CD105 was measured by the standard immunohistochemical staining procedure. The mean value of MVD in OLP patients was significantly higher than that in the controls (14.61±12.48 vs. 8.67±1.76, P<0.0001). Furthermore, there was a significant difference in the mean MVD value between reticular and atrophic/erosive lesions (8.19±7.13 vs. 25.73±12.06, P=0.001). However, no significant difference was observed between the reticular lesions and normal tissues (P=0.58). An increased level of CD105 in OLP patients can improve our knowledge about the causes and mechanisms of the disease. The CD105-MVD assessment might be a useful method for semiquantitative measurement of angiogenesis in OLP patients as well as differentiating its clinical forms; therefore, it can open new vistas for formulating strategies based on antiangiogenic treatments for the management of OLP and other precancerous lesions.

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Long Non-Coding RNA- PVT1 in Ovarian Cancer as Predictors for Platinum Analogues Resistance

QJM ◽

10.1093/qjmed/hcab103.002 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Walid Abd Almonem Biomy ◽

Mohamed Yassin Mostafa ◽

Nashwa Nagy EL-Khazragy ◽

Khaled Kamal Eldein Ghonem ◽

Amr Attia Hewety

Keyword(s):

Ovarian Cancer ◽

Retrospective Study ◽

Cancer Patients ◽

Complete Blood Count ◽

Ovarian Tissue ◽

Treatment Modalities ◽

Control Group ◽

Presenting Symptoms ◽

Significant Difference ◽

Normally Distributed

Abstract Background Ovarian cancer is the fifth most common type of cancer and the most common cause of death among women with gynecological malignancies. Objectives The aim of this retrospective study was to assess expression of the Lnc_PVT1 in the studied subjects. The expression of Lnc_PVT1 biomarker; was compared in ovarian cancer patients versus the non-cancerous tissue. The data normalization was tested by normality test which shows that the biomarker expression is not normally distributed; thus, a Mann Whitney statistics test for non -parametric values was applied. Our results showed a high significant difference in the expression of Lnc-PVT1 (p < 0.01). Lnc_PVT1 expression is upregulated by 2.4 folds in ovarian cancer patients (median: 32.0; range: 11 – 92) compared to control group (median: 13.0; range: 11 – 16). Methods and Materials This is a retrospective study. Include patients with advanced epithelial ovarian cancer (stage II and IV) who received chemotherapy platinum based and taxines from 2014-2017. Setting: Clinical oncology department Ain Shams University, Egypt, Demerdash hospital. Data collection: The following information will be ANONYMOUSLY extracted from the patient medical files: age at diagnosis, presenting symptoms, laboratory investigations: (Liver function, kidney function, complete blood count &tumor markers), tumor histological type, tumor FIGO stage& degree of differentiation, treatment modalities, type of surgery, chemotherapy regimens (Adjuvant, Neoadjuvant or palliative), chemotherapy responsiveness or resistance, disease recurrence and disease-free period, and patients' survival. Results The current study was enrolled on 55 subjects, they classified into two subgroups. The ovarian cancer cases constitute 45 patients; samples collected from patients after diagnosis has been confirmed by histopathology based on immune-histochemical analysis as well as Computerized axial tomography (CT scan) and Magnetic resonance imaging (MRI). The cancer ovary patients were compared to ten non- malignant ovarian tissues. Table 1 illustrates the demographic and clinical data of the studied subjects. We test the normality for age; the data was normally distributed. For this reason, we represent the data in mean value and standard deviation form. Matched age and gender was observed between ovarian cancer patients and healthy control. The mean age for the studied subjects was 47.7±9.1. In our study; the Cisplatin resistant patients (n = 25) had a mean age of 46±5 compared to Cisplatin sensitive which were older (mean: 50 ±11.5). Regarding the age subgroups; the majority of the studied subjects were younger than 48 years old. The ovarian cancer patients were subcategorized into subgroups (Cisplatin sensitive/resistant). Conclusion Comparative analysis between Cisplatin sensitive and resistant ovarian cancer tissues for the Lnc_PVT1, TGF-b and Caspase-3. A high significant difference was observed by Mann- Whitney test (p = 0.001) between Cisplatin sensitive and resistant ovarian cancer patients; Cisplatin resistant tissues showed higher expression levels of the Lnc_PVT1 expression (median 20.0, range: 10 - 92) in Cisplatin sensitive ovarian tissue compared to Cisplatin resistant tissue (median: 83; range: 46-86).

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The effect of different doeses of aspirin application on oxidative stress in ovarian tissue

Medical Science and Discovery ◽

10.36472/msd.v8i8.585 ◽

2021 ◽

Vol 8 (8) ◽

pp. 475-479

Author(s):

Deniz Dirik ◽

Ahmet Ufuk Komuroglu

Keyword(s):

Oxidative Stress ◽

Ovarian Tissue ◽

Thiol Group ◽

Special Treatment ◽

Control Group ◽

Albino Rats ◽

Positive Effects ◽

Significant Difference ◽

Group 5 ◽

Different Doses

Objective: Aspirin is a non-steroidal anti-inflammatory drug with antioxidative properties. It is recommended to use different doses and durations according to the characteristics of the patient and the type of disease. Therefore, in this study, we aimed to investigate the effect of using aspirin at different doses and for different durations on oxidative stress in ovarian tissue. Material and Methods: Female Wistar albino rats were divided into five groups. Group 1: control group, no special treatment was applied to the rats in this group. Group 2: 1 mg/kg aspirin was administered orally to the rats in this group every day for 28 days. Group 3: 3 mg/kg aspirin was administered orally to rats in this group every three days. Ggroup 4: 5 mg/kg aspirin was administered orally to rats in this group every five days. Group 5: 7 mg/kg aspirin was administered orally to the rats in this group once a week. After fasting overnight following the last application, the rats were sacrificed, and their ovarian tissues were collected. Malondialdehyde, catalase, total thiol group, and AOPP levels were studied from ovarian tissue. Results: Group4 and group5 ovarian tissue MDA levels were found to be significantly higher than the other groups (p<0.05). There was no significant difference between group1, group2 and group3 ovarian tissue MDA levels (p>0.05). Group1 (control group) ovarian tissue AOPP level was found to be significantly lower than all aspirin-administered groups (p<0.05). Group2 ovarian tissue AOPP level was found to be significantly lower than group3, group4 and group5 (p<0.05). TSG level was found to be significantly higher in group 5 when compared to other groups (p0<0.05). Group4 ovarian tissue TSG level was found to be significantly higher when compared to group1, group2 and group3 (p<0.05). Group3 and group4 ovarian tissue CAT activity was found to be significantly higher than group1, group2 and group5 (p<0.05). When group1, group2 and group5 ovarian tissue CAT activities were compared, no significant difference was found (p>0.05). Conclusion: The application of aspirin at certain intervals rather than daily application may have more positive effects on the antioxidant system. especially taking aspirin at intervals of 3 or 5 days may be more effective

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PENGARUH EKSTRAK KULIT BUAH NAGA MERAH (HYLOCEREUS POLYRHIZUS) TERHADAP OVARIUM MENCIT MODEL ENDOMETRIOSIS

EMBRIO ◽

10.36456/embrio.vol9.no1.a720 ◽

2017 ◽

Vol 9 ◽

pp. 10-15

Author(s):

Sagita candra Puspitasari

Keyword(s):

Treatment Group ◽

Granulosa Cell ◽

Cell Apoptosis ◽

Ovarian Follicle ◽

The Body ◽

Control Group ◽

Mice Model ◽

Fruit Peel ◽

Dragon Fruit ◽

Significant Difference

Endometriosis occurs when one of the normal tissues grows ectopically on the organs of the body other than the uterus. One of the mechanisms of infertility associated with endometriosis is a change in immune system function that affects the ovarian follicle which causes an increase in granulosa cells in the ovarian follicle endometriosis patients. The red dragon fruit is one of the plants capable of inhibiting NF-ÎºB secretion of cytotine from endometriosis. This study aims to study the effect of red dragon fruit extract on the ovaries of mice of endometriosis model. 12 samples of female mice divided into 2 groups consisting of positive control group and treatment group used as endometriosis for 14 days, then the next 14 days positive group is given solution Na-CMC 0,5%, while treatment group given dose 0,25 mg / gram BB. This research type is true experimental by using randomized post test only control experimental group design. The results of this study indicate that there is a significant difference in the number of granulosa cell apoptosis between groups. Provision of extract dragon fruit peel in mice model of endometriosis can decrease the number of granulosa cell apoptosis with significant result P <0,05. Keywords : extract of hylocereus polyrhizuspeel, endometriosis, granulose cell apoptotic

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Levels of Prothrombin Fragment F1+2 in Patients with Hyperhomocysteinemia and a History of Venous Thromboembolism

Thrombosis and Haemostasis ◽

10.1055/s-0038-1665405 ◽

1997 ◽

Vol 78 (05) ◽

pp. 1327-1331 ◽

Cited By ~ 21

Author(s):

Paul A Kyrle ◽

Andreas Stümpflen ◽

Mirko Hirschl ◽

Christine Bialonczyk ◽

Kurt Herkner ◽

...

Keyword(s):

Venous Thromboembolism ◽

Thrombin Generation ◽

Oral Anticoagulant Therapy ◽

Oral Anticoagulants ◽

Control Group ◽

Prothrombin Fragment ◽

Hemostatic System ◽

Significant Difference ◽

System Activation ◽

One Year

SummaryIncreased thrombin generation occurs in many individuals with inherited defects in the antithrombin or protein C anticoagulant pathways and is also seen in patients with thrombosis without a defined clotting abnormality. Hyperhomocysteinemia (H-HC) is an important risk factor of venous thromboembolism (VTE). We prospectively followed 48 patients with H-HC (median age 62 years, range 26-83; 18 males) and 183 patients (median age 50 years, range 18-85; 83 males) without H-HC for a period of up to one year. Prothrombin fragment Fl+2 (Fl+2) was determined in the patient’s plasma as a measure of thrombin generation during and at several time points after discontinuation of secondary thromboprophylaxis with oral anticoagulants. While on anticoagulants, patients with H-HC had significantly higher Fl+2 levels than patients without H-HC (mean 0.52 ± 0.49 nmol/1, median 0.4, range 0.2-2.8, versus 0.36 ± 0.2 nmol/1, median 0.3, range 0.1-2.1; p = 0.02). Three weeks and 3,6,9 and 12 months after discontinuation of oral anticoagulants, up to 20% of the patients with H-HC and 5 to 6% without H-HC had higher Fl+2 levels than a corresponding age- and sex-matched control group. 16% of the patients with H-HC and 4% of the patients without H-HC had either Fl+2 levels above the upper limit of normal controls at least at 2 occasions or (an) elevated Fl+2 level(s) followed by recurrent VTE. No statistical significant difference in the Fl+2 levels was seen between patients with and without H-HC. We conclude that a permanent hemostatic system activation is detectable in a proportion of patients with H-HC after discontinuation of oral anticoagulant therapy following VTE. Furthermore, secondary thromboprophylaxis with conventional doses of oral anticoagulants may not be sufficient to suppress hemostatic system activation in patients with H-HC.

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