scholarly journals Dietary Analysis Based on 18S rDNA, and Stable Carbon and Nitrogen Isotopes in Juvenile Eriocheir sinensis Crabs Reared Under Three Feeding Modes

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhenzhen Lu ◽  
Yunfei Sun ◽  
Changlun Xiao ◽  
Yongxu Cheng
Keyword(s):  
Nitrogen Isotopes ◽  
Feeding Habits ◽  
Eriocheir Sinensis ◽  
Alternanthera Philoxeroides ◽  
Feeding Mode ◽  
Dietary Analysis ◽  
Male And Female ◽  
Feeding Modes ◽  
Mitten Crab ◽  
Significant Difference

To investigate the growth and feeding conditions of the Chinese mitten crab Eriocheir sinensis under different feeding modes: traditional (mainly consisting of wheat, bran, and soybean meal), formulated, and mixed feeds (1:1 mixture of traditional and formulated feeds) were fed in different crab breeding ponds in this study. During the experiment, the stomach contents of juvenile crabs under the different feeding modes were collected. The main potential eukaryotic food components were studied using 18S ribosomal DNA sequencing, and the contribution of different feeding modes to the feeding source of juvenile crabs were analyzed using C and N stable isotopes. The terminal weight and weight gain rate of crabs under the formulated feeding mode were significantly higher than those in the traditional and mixed feeding modes (P < 0.05). No differences were observed in the diversity and abundance of the main potential eukaryotic feed components of male and female crabs under different feeding modes (P > 0.05). Thirty-four phyla, composed mainly of benthic organisms, were identified, with Arthropoda (mainly including Malacostraca, 30.25–51.48%), Phragmoplastophyta (mainly including Embryophyta and Trebouxiophyceae, 5.08–24.74%), and Diatomea (3.13–8.43%) being the most abundant. The δ13C and δ15N values of the feeding sources and muscle of crabs ranged from −34.45 to −22.21‰, and from 0.27 to 5.66‰, respectively, varying greatly among the three feeding modes and δ15N value of muscle under formulated feeding mode was significantly higher than that in traditional feeding mode (P < 0.05). The proportion of particulate organic matter (11.92–17.50%) is similar to Alternanthera philoxeroides (11.24–16.03%) in three feeding modes. There was no significant difference in feeding habits between male and female crabs under the same and different feeding modes. Juvenile crabs feed on both plant- and animal-based feeds in an aquaculture pond, but they are not complete predators and selectively feed on animal or plant feeds as supplements of that which is deficient, in addition to their main feed.

Gill ATPase Activities and Microstructures of Juvenile Chinese Mitten Crab, Eriocheir sinensis, Exposed to Ambient Mercury

2011 ◽  
Vol 183-185 ◽  
pp. 1543-1548
Author(s):  
Yan Min Zhao ◽  
Xin Hua Wang ◽  
Yan Wen Qin ◽  
Bing Hui Zheng ◽  
Lei Zhang
Keyword(s):  
Exposure Time ◽  
Mercury Concentration ◽  
Significant Interaction ◽  
Eriocheir Sinensis ◽  
Structure Change ◽  
Microstructure Analysis ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Significant Difference

Experiments were carried out to investigate the effects of ambient mercury (Hg2+) on gill Na+-K+-ATPase, Ca2+-Mg2+-ATPase activities, and gill microstructure of juvenile Chinese mitten crab, Eriocheir sinensis. Crabs were exposed to ambient 0.00, 0.01, 0.05, 0.10, 0.20 and 0.30 mg/l Hg2+, and sampled on the days of 1, 7, 14 and 30 respectively. The results showed that both ambient Hg2+ concentrations and exposure time had significant effects on both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of E. sinensis, which showed the tendency to decrease with ambient mercury concentration increasing and exposure time prolonging. While for crabs exposed to 0.01 mg/l Hg2+, no significant difference was observed when compared with the control. Significant interaction between ambient Hg2+ and exposure time on these two ATPases activities was also observed with the lowest values found in crabs exposed to 0.30 mg/l Hg2+ for 30 days. The anterior gill microstructure analysis of E. sinensis exposed to different ambient Hg2+ for 30 days showed that mercury could alter the gill microstructures of E. sinensis. However, there was no significantly structure change appeared in crabs exposed to 0.01 mg/l Hg2+. Combined, these results indicated that though ambient Hg2+ could lead to significant decrease of both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, and could destroy the gill microstructures, the crab of E. sinensis could acclimate to low Hg2+ contamination less than 0.01mg/l.

Feeding history affects the crabseed quality and subsequent culture performance of juvenile Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Brachyura, Varunidae)

Crustaceana ◽  
2021 ◽  
Vol 94 (1) ◽  
pp. 97-114
Author(s):  
Xiaodong Jiang ◽  
Yuhong Yang ◽  
Yongxu Cheng ◽  
Xugan Wu
Keyword(s):  
Pathogen Resistance ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Feeding Trial ◽  
Formulated Diets ◽  
Mitten Crab ◽  
Trash Fish ◽  
Significant Difference ◽  
Traditional Diets

Abstract A 6-month feeding trial was conducted to evaluate the hepatopancreas colour, proximate composition, antioxidant capacity, pathogen resistance and growth performance of juvenile Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 fed either formulated diets (FD), traditional diets (TD) or a mixture feeding of traditional diets and frozen trash fish (TF). The results showed that: (1) The hepatopancreas redness (a*) in the FD treatment was significantly higher than that in the other two treatments (). (2) The significantly higher activities of alkaline phosphatase (AKP), acid phosphatase (ACP) and superoxide dismutase (SOD) were observed in the hepatopancreas in the FD treatment, while a significantly higher content of malondialdehyde (MDA) was observed in the haemolymph in the TF treatment (). (3) FD and TD treatment, respectively, had the lowest and the highest mortality after challenging with Aeromonas hydrophila. (4) Females in the FD treatment had a significantly shorter intermoult period in the indoor culture experiment (), no significant difference was observed in weight gain rate, specific growth rate, or survival. In conclusion, formulated diets could enhance the crabseed quality of E. sinensis compared to traditional diets or trash fish.

Comparison of Volatile Compounds in Raw and Cooked Chinese Mitten Crab Hepatopancreas and Gonads

2014 ◽  
Vol 941-944 ◽  
pp. 1040-1044 ◽  
Author(s):  
Xian Chu Gao ◽  
Sai Qi Gu ◽  
Xi Chang Wang ◽  
Ning Ping Tao ◽  
Jing Zhuang ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Chinese Mitten Crab ◽  
Active Compounds ◽  
Male And Female ◽  
Mitten Crab ◽  
Significant Difference ◽  
Crab Hepatopancreas ◽  
New Type ◽  
Edible Parts

The volatile compounds in different edible parts (gonads, hepatopancreas) of raw and cooked Chinese mitten crab were extracted by a new type material (MonoTrap) and analyzed by gas chromatography-mass spectrometry (GC-MS). Totally 97 volatile compounds were identified by GC-MS and their odor characteristics were analyzed by ROAV values screen. There is a significant difference of odor-active compounds between male and female Chinese mitten crab. What’s more, the results indicated that the varieties and contents of volatile compounds in hepatopancreas were greater than in gonad. The results of ROAV values screen were summarized, 15 odor-active compounds whose ROAV values greater than 0.1and 9 key odor-active compounds whose ROAV values greater than 1 were further selected. The results indicated that decanal, trimethylamine, nonanal were the key odor-active compounds in hepatopancreas and gonads.

scholarly journals The Gills Area of Two Species of Marine Water Crabs: The Blue Swimming Crab, Portunus pelagicus (Linnaeus, 1758), and the Chinese Mitten Crab, Eriocheir sinensis H. Milne Edwards, 1853 from the North- West Arabian Gulf and Shatt Al-Arab River , Iraq.

2019 ◽  
Vol 32 ◽  
pp. 292-301
Author(s):  
Intisar M.A. Jabbar ◽  
Salah M. Najim ◽  
Entesar N. Sultan
Keyword(s):  
Surface Area ◽  
Arabian Gulf ◽  
Dry Weight ◽  
Eriocheir Sinensis ◽  
Portunus Pelagicus ◽  
North West ◽  
The North ◽  
Mitten Crab ◽  
Significant Difference ◽  
Gill Surface Area

The present study was conducted on the gills structure and the gill surface area of 40 individuals of the Blue swimming crabs, Portunus pelagicus, and 40 Chinese mitten crabs, Eriocheir sinensis, specimens of the species were collected from the  different regions of Shatt Al- Arab and North-west Arabian gulf. Both species were having eight phyllobranchiatesas of the same structure but different in length and surface area. The second gill in P. pelagicus shows major difference in shape from the other gills with missing platelets from one side of the filament. The results showed there were positive relationships between dry body weight with gill surface area, total number of platelets and number of platelets surface area in each gill. The gill surface area had no significant difference (p>0.05) with dry weight in the two species.

Effects of 3 Feeding Modes on the Volatile and Nonvolatile Compounds in the Edible Tissues of Female Chinese Mitten Crab (Eriocheir sinensis)

2016 ◽  
Vol 81 (4) ◽  
pp. S968-S981 ◽  
Author(s):  
Kejin Zhuang ◽  
Na Wu ◽  
Xichang Wang ◽  
Xugan Wu ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Feeding Modes ◽  
Mitten Crab ◽  
Edible Tissues

Utilizing next-generation sequencing to identify prey DNA in western North Atlantic grey seal Halichoerus grypus diet

2020 ◽  
Vol 655 ◽  
pp. 227-240
Author(s):  
KR Flanders ◽  
ZH Olson ◽  
KA Ono
Keyword(s):  
Next Generation Sequencing ◽  
Feeding Habits ◽  
Atlantic Menhaden ◽  
Halichoerus Grypus ◽  
Frequency Of Occurrence ◽  
Grey Seal ◽  
Next Generation ◽  
Significant Difference ◽  
Generation Sequencing ◽  
Seal Diet

Increasing grey seal Halichoerus grypus abundance in coastal New England is leading to social, political, economic, and ecological controversies. Central to these issues is the foraging ecology and diet composition of the seals. We studied grey seal feeding habits through next-generation sequencing of prey DNA using 16S amplicons from seal scat (n = 74) collected from a breeding colony on Monomoy Island in Massachusetts, USA, and report frequency of occurrence and relative read abundance. We also assigned seal sex to scat samples using a revised PCR assay. In contrast to current understanding of grey seal diet from hard parts and fatty acid analysis, we found no significant difference between male and female diet measured by alpha and beta diversity. Overall, we detected 24 prey groups, 18 of which resolved to species. Sand lance Ammodytes spp. were the most frequently consumed prey group, with a frequency of occurrence (FO) of 97.3%, consistent with previous studies, but Atlantic menhaden Brevoortia tyrannus, the second most frequently consumed species (FO = 60.8%), has not previously been documented in US grey seal diet. Our results suggest that a metabarcoding approach to seal food habits can yield important new ecological insights, but that traditional hard parts analysis does not underestimate consumption of Atlantic cod Gadus morhua (FO = 6.7%, Gadidae spp.) and salmon Salmo salar (FO = 0%), 2 particularly valuable species of concern.

SENSITIVITAS ETIS MAHASISWA ATAS PENGGELAPAN PAJAK

2017 ◽  
Vol 8 (2) ◽  
pp. 178-195
Author(s):  
Nurma Risa
Keyword(s):  
Tax Evasion ◽  
Female Students ◽  
T Test ◽  
Study Program ◽  
Male And Female ◽  
Political Sciences ◽  
Significant Difference ◽  
The Subject ◽  
And Gender ◽  
Management Students

This study aims to prove that there is a difference of perception about ethics on tax evasion in UNISMA Bekasi students, based on selected study program and gender. The sample of this research is the students who have fulfilled the subject of taxation, at the Faculty of Economics (FE) and Faculty of Social and Political Sciences (FISIP). Using independent t-test, the results showed that there was no significant difference of perception about tax evasion ethics between FE and FISIP students. But significant differences the perception of tax evasion ethics occur between accounting and management students at FE. Significant differences also did not occur between male and female students

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