Comparison of Volatile Compounds in Raw and Cooked Chinese Mitten Crab Hepatopancreas and Gonads

2014 ◽  
Vol 941-944 ◽  
pp. 1040-1044 ◽  
Author(s):  
Xian Chu Gao ◽  
Sai Qi Gu ◽  
Xi Chang Wang ◽  
Ning Ping Tao ◽  
Jing Zhuang ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Chinese Mitten Crab ◽  
Active Compounds ◽  
Male And Female ◽  
Mitten Crab ◽  
Significant Difference ◽  
Crab Hepatopancreas ◽  
New Type ◽  
Edible Parts

The volatile compounds in different edible parts (gonads, hepatopancreas) of raw and cooked Chinese mitten crab were extracted by a new type material (MonoTrap) and analyzed by gas chromatography-mass spectrometry (GC-MS). Totally 97 volatile compounds were identified by GC-MS and their odor characteristics were analyzed by ROAV values screen. There is a significant difference of odor-active compounds between male and female Chinese mitten crab. What’s more, the results indicated that the varieties and contents of volatile compounds in hepatopancreas were greater than in gonad. The results of ROAV values screen were summarized, 15 odor-active compounds whose ROAV values greater than 0.1and 9 key odor-active compounds whose ROAV values greater than 1 were further selected. The results indicated that decanal, trimethylamine, nonanal were the key odor-active compounds in hepatopancreas and gonads.

Analysis of Key Odor Compounds in Steamed Chinese Mitten Crab (Eriocheir sinensis)

2014 ◽  
Vol 941-944 ◽  
pp. 1026-1035
Author(s):  
Sai Qi Gu ◽  
Na Wu ◽  
Xi Chang Wang ◽  
Jing Jing Zhang ◽  
Si Ru Ji
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Eriocheir Sinensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Gas Chromatography Olfactometry ◽  
Odor Compounds ◽  
New Type ◽  
Edible Parts

Key odor compounds in different edible parts (abdomen, claw, leg meat and gonad) of steamed female Chinese mitten crab (Eriocheir sinensis) were extracted by a new type of adsorbent (MonoTrap), identified by gas chromatography-mass spectrometry (GC-MS) and determined by gas chromatography-olfactometry (GC-O). Ninteen key odor compounds (odor intensity value≥3) were obtained in Chinese mitten crab, among which trimethylamine (fishy), (Z)-4-heptenal (fishy) and 2-ethylpyridine (roasted potato) were found in all four edible parts of Eriocheir sinensis. In addition, 8 KOCs (2-ethylfuran, benzaldehyde, 1-octen-3-ol, 6-methyl-2-heptanone, 2-pentylfuran, (E,E)-3,5-octadien-2-one, nonanal and decanal) were also identified in the abdomen meat, 5 KOCs (toluene, 2,5-dimethylpyrazine, 1-octen-3-ol, (E,E)-3,5-octadien-2-one and 2-methylnaphthalene) in the claw meat, 3 KOCs (toluene, 2-nonanone and nonanal) in the leg meat, and 12 KOCs (1-penten-3-ol, toluene, hexanal, 1,3,5-octatriene, benzaldehyde, 1-octen-3-ol, 6-methyl-2-heptanone, 2-pentylfuran, (E,E)-3,5-octadien-2-one, nonanal, (E,Z)-2,6-nonadienal and decanal) in the gonad part.

scholarly journals Lipometabolic Alteration in Mice Feeding Eatable Tissues of Chinese Mitten Crab

2016 ◽  
Vol 9 (1) ◽  
pp. 195
Author(s):  
Jun Jing ◽  
Wenhui Wu ◽  
Xinfeng Xiao ◽  
Yu Zhou ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Lipid Levels ◽  
Chinese Mitten Crab ◽  
Male Crab ◽  
Mitten Crab ◽  
Crab Hepatopancreas ◽  
Edible Parts ◽  
Blood Lipid Levels

<p>Objective:<strong> </strong>Chinese mitten crab<em> </em>is a famous aquatic species in eastern Asian region, but their edible parts, particularly hepatopancreas and gonads, generally contain very high levels of lipids that may have negative effects on human health. This study investigated the effects of different edible parts of Chinese mitten crab on the body weight and lip metabolism for Kunming mice.</p><p>Method: The mice were fed with diets containing one part of an Chinese mitten crab or the mixture of parts of an Chinese mitten crab for 4 weeks. There were 9 treatments. The triacylglycerol (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were enzymatically determined using commercial kits (purchased from Nanjing Jiancheng Bioengineering Institute, China). The arteriosclerosis index (AI) was calculated by the equation: AI = (TC – HDL-C)/HDL-C. The levels of fatty acid syntheses (FAS), the 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMG-CoA) and lipoprotein lipase (LPL) were measured using commercially available kits according to the manufacturer’s instructions. The significant differences between the groups were further analyzed by Bonferronis’s t-test.</p><p>Results: Our results showed that the crab hepatopancreas, gonads and the mixed male crab-edible parts increased blood lipids in some experiment group of mice corresponding to a change in the nutrition-related liver enzymes. It shows that addition of the Chinese mitten crab has an adverse effect on the blood lipid levels in mice. The FFH, FFMI and FMMI groups had significantly higher weight than the FN group (P &lt; 0.05). The crab hepatopancreas, crab gonads and the mixed male crab-edible parts cause an increase in the blood lipid levels. The crab mixture significantly affected the AI value of male and female mice (P &lt; 0.01). The level of FMMI group was significantly higher than the FN group (P &lt; 0.05). Other groups showed no significant difference. The level of the FFMI group was significantly lower than the FN group (P &lt; 0.05), and levels in the MMM and MFMI groups were significantly lower than the MN group (P &lt; 0.05).<strong></strong></p><p>Conclusion: It clearly showed that long-term feeding with the Chinese mitten crab has an adverse effect on the blood lipid levels in mice. One the one hand, the weight, liver index and fat index of experimental mice were changed than normal mice. On the other hand, the crab diet affects the level of TC, TG, AI and FASN on increasing. It is suggested that the special diet has affected lip metabolic alteration associated with contents of serum lipids and metabolic enzymes. But according to a certain regular feeding, there would be no adverse effect on mice. On the contrary, it may adjust the blood lipid in mice<strong> </strong></p>

scholarly journals A Study on the Volatile Compounds in Elaeagnus angustifolia L. Flowers during Flowering Season by Gas Chromatography-Mass Spectrometry Coupled with Advanced Chemometrics

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Zhijing Liu ◽  
Degang Ding ◽  
Huapeng Cui ◽  
Chuanchuan Wang ◽  
Juntao Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Analysis Tool ◽  
Elaeagnus Angustifolia ◽  
Full Bloom ◽  
Chromatography Mass Spectrometry ◽  
Flowering Season ◽  
Significant Difference

The flowers of Elaeagnus angustifolia L. have been used as a homologous variety in China, whose quality seriously relies on the compositions during the flowering period. Unfortunately, studies on the variations of volatile compounds during the flowering season are rarely reported. Herein, a gas chromatography-mass spectrometry-based untargeted metabolomic methodology was proposed for the comprehensive analysis of volatile compounds in E. angustifolia flowers to classify various flowering stages. Samples from four flowering stages were collected, including the initial bloom stage, pre-full bloom stage (70–80% of flowers), full bloom stage, and ending of the bloom stage. Simultaneous distillation extraction was used for the extraction of volatile compounds in the flowers, which was then analyzed by a newly developed chemometric data analysis tool, autoGCMSDataAnal. An advantage of the developed methodology is that compounds can be accurately screened and identified. Finally, 59 compounds that showed significant difference among four flowering stages were screened and 31 compounds were identified. Sample clustering results from principal component analysis and hierarchical clustering analysis suggested that flowers from the pre-full bloom stage and full bloom stage may be more suitable when used as raw materials for industrial products.

Gill ATPase Activities and Microstructures of Juvenile Chinese Mitten Crab, Eriocheir sinensis, Exposed to Ambient Mercury

2011 ◽  
Vol 183-185 ◽  
pp. 1543-1548
Author(s):  
Yan Min Zhao ◽  
Xin Hua Wang ◽  
Yan Wen Qin ◽  
Bing Hui Zheng ◽  
Lei Zhang
Keyword(s):  
Exposure Time ◽  
Mercury Concentration ◽  
Significant Interaction ◽  
Eriocheir Sinensis ◽  
Structure Change ◽  
Microstructure Analysis ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Significant Difference

Experiments were carried out to investigate the effects of ambient mercury (Hg2+) on gill Na+-K+-ATPase, Ca2+-Mg2+-ATPase activities, and gill microstructure of juvenile Chinese mitten crab, Eriocheir sinensis. Crabs were exposed to ambient 0.00, 0.01, 0.05, 0.10, 0.20 and 0.30 mg/l Hg2+, and sampled on the days of 1, 7, 14 and 30 respectively. The results showed that both ambient Hg2+ concentrations and exposure time had significant effects on both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of E. sinensis, which showed the tendency to decrease with ambient mercury concentration increasing and exposure time prolonging. While for crabs exposed to 0.01 mg/l Hg2+, no significant difference was observed when compared with the control. Significant interaction between ambient Hg2+ and exposure time on these two ATPases activities was also observed with the lowest values found in crabs exposed to 0.30 mg/l Hg2+ for 30 days. The anterior gill microstructure analysis of E. sinensis exposed to different ambient Hg2+ for 30 days showed that mercury could alter the gill microstructures of E. sinensis. However, there was no significantly structure change appeared in crabs exposed to 0.01 mg/l Hg2+. Combined, these results indicated that though ambient Hg2+ could lead to significant decrease of both gill Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, and could destroy the gill microstructures, the crab of E. sinensis could acclimate to low Hg2+ contamination less than 0.01mg/l.

scholarly journals Dietary Analysis Based on 18S rDNA, and Stable Carbon and Nitrogen Isotopes in Juvenile Eriocheir sinensis Crabs Reared Under Three Feeding Modes

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhenzhen Lu ◽  
Yunfei Sun ◽  
Changlun Xiao ◽  
Yongxu Cheng
Keyword(s):  
Nitrogen Isotopes ◽  
Feeding Habits ◽  
Eriocheir Sinensis ◽  
Alternanthera Philoxeroides ◽  
Feeding Mode ◽  
Dietary Analysis ◽  
Male And Female ◽  
Feeding Modes ◽  
Mitten Crab ◽  
Significant Difference

To investigate the growth and feeding conditions of the Chinese mitten crab Eriocheir sinensis under different feeding modes: traditional (mainly consisting of wheat, bran, and soybean meal), formulated, and mixed feeds (1:1 mixture of traditional and formulated feeds) were fed in different crab breeding ponds in this study. During the experiment, the stomach contents of juvenile crabs under the different feeding modes were collected. The main potential eukaryotic food components were studied using 18S ribosomal DNA sequencing, and the contribution of different feeding modes to the feeding source of juvenile crabs were analyzed using C and N stable isotopes. The terminal weight and weight gain rate of crabs under the formulated feeding mode were significantly higher than those in the traditional and mixed feeding modes (P &lt; 0.05). No differences were observed in the diversity and abundance of the main potential eukaryotic feed components of male and female crabs under different feeding modes (P &gt; 0.05). Thirty-four phyla, composed mainly of benthic organisms, were identified, with Arthropoda (mainly including Malacostraca, 30.25–51.48%), Phragmoplastophyta (mainly including Embryophyta and Trebouxiophyceae, 5.08–24.74%), and Diatomea (3.13–8.43%) being the most abundant. The δ13C and δ15N values of the feeding sources and muscle of crabs ranged from −34.45 to −22.21‰, and from 0.27 to 5.66‰, respectively, varying greatly among the three feeding modes and δ15N value of muscle under formulated feeding mode was significantly higher than that in traditional feeding mode (P &lt; 0.05). The proportion of particulate organic matter (11.92–17.50%) is similar to Alternanthera philoxeroides (11.24–16.03%) in three feeding modes. There was no significant difference in feeding habits between male and female crabs under the same and different feeding modes. Juvenile crabs feed on both plant- and animal-based feeds in an aquaculture pond, but they are not complete predators and selectively feed on animal or plant feeds as supplements of that which is deficient, in addition to their main feed.

scholarly journals Volatile compounds of Macedonian fermented sausage as affected by ripening process using SPME/GC-MS

2020 ◽  
Vol 26 (1) ◽  
pp. 71-78
Author(s):  
Erhan Sulejmani ◽  
Muhamet Demiri
Keyword(s):  
Volatile Compounds ◽  
Solid Phase ◽  
Total Concentration ◽  
Meat Product ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Fermented Sausage ◽  
Significant Difference ◽  
Aromatic Components ◽  
Production Stages

The profiles of volatile compounds of Macedonian dry fermented sausage were determined by gas chromatography-mass spectrometry (GC?MS) using a solid- -phase microextraction (SPME). A total of 103 volatile compounds were identified and consisted of 12 acids, 16 ketones, 21 terpenes, 20 alcohols, 9 esters, 13 hydrocarbons and 12 miscellaneous. The analysis of the main volatile aromatic components proved that 62% of the compounds that were detected on the first days are terpenes and their content increased to 69 and 80% on days eight and fifteen, respectively. The 50% of the total concentration of volatile components results to be concentrated on the last day (15th) of ripening. They have the highest amounts of concentration of acids (57%), ketones (68%) and terpenes (56%) from the total concentration. There was a significant difference between the various periods of fermentation and there can be differences in the manufacture production stages. Macedonian dry sausages contain high levels of spicy terpenes, which could play an important role in the general flavour notes of this meat product.

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