scholarly journals Growth and Lipidomic Analyses of Penaeus monodon Larvae Supplemented With Aurantiochytrium limacinum BCC52274

2021 ◽  
Vol 8 ◽  
Author(s):  
Virak Visudtiphole ◽  
Jutatip Khudet ◽  
Panomkorn Chaitongsakul ◽  
Siwat Plaisen ◽  
Jittima Siriwattano ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Penaeus Monodon ◽  
Docosapentaenoic Acid ◽  
Dietary Fatty Acids ◽  
Lipid Classes ◽  
Marine Animals ◽  
Supplementation Period ◽  
Marked Accumulation ◽  
Insight Into ◽  
Average Body

Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential for growth and health of larval marine animals. Marine animals have a limited capability for LC-PUFA synthesis, and the larvae must obtain LC-PUFAs from diet. The protist Aurantiochytrium limacinum (AL) is abundant in 22:6 n-3 (docosahexaenoic acid, DHA), 22:5 n-3 (docosapentaenoic acid, DPA) and 16:0 fatty acids, which qualifies it as an LC-PUFA source for feed application. Therefore, in this study, a common feed containing lower amounts of total LC-PUFAs, Thalasiosira weissflogii, was replaced with AL at graded proportions and supplied to Penaeus monodon larvae from mysis (M) 1 to post-larval (PL) 2 stages to supplement LC-PUFAs in the diet. After that, all shrimp from PL2 to PL12 were continuously reared and subjected to the same diet regime, which was a combination of Artemia and commercial dried feed. The AL-supplemented PL2 shrimp demonstrated marked accumulation of the key fatty acids present in AL—16:0, DPA and DHA. The supplemented larvae showed no difference in growth during the supplementation period from M1 to PL2; however, average body weight and biomass were increased in PL12 shrimp that were fed earlier with AL. Lipidomic analysis revealed that profiles of fatty acids but not lipid classes/subclasses in PL shrimp reflected the supplied diet. The main saturated fatty acid (SFA, 16:0) predominantly accumulated in acylglycerols, which are energy-reserve lipids, in PL2 shrimp. Both LC-PUFAs (DHA and DPA) were preferentially deposited in phospholipids or structural lipids. Furthermore, while the amounts of both LC-PUFAs increased along with the amount of supplied AL, that of the SFA did not. This suggests that LC-PUFAs were prioritized to be stored over SFA when both types of fatty acids were present in high amounts. This analysis substantiates the importance of LC-PUFAs and provides an insight into how different types of the dietary fatty acids were differentially accumulated in lipid classes and subclasses for their biological functions.

scholarly journals Deposition of dietary fatty acids, de novo synthesis and anatomical partitioning of fatty acids in finishing pigs

2007 ◽  
Vol 97 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Maela Kloareg ◽  
Jean Noblet ◽  
Jaap van Milgen
Keyword(s):  
Fatty Acids ◽  
De Novo ◽  
Docosapentaenoic Acid ◽  
Quantitative Information ◽  
Point Of View ◽  
Dietary Fatty Acids ◽  
Whole Body ◽  
Finishing Pigs ◽  
Average Composition ◽  
Fa Composition

Predicting aspects of pork quality becomes increasingly important from both a nutritional and technological point of view. The aim of the present study was to provide quantitative information on the relation between nutrient intake and whole-body fatty acid (FA) deposition. This information is essential to develop mechanistic models predicting the FA content of tissues. A serial slaughter study was carried out in which thirty pigs were slaughtered between 90 and 150 kg. The diet included 15 g/kg soyabean oil and contained 44 g/kg fat. Only 0·31 and 0·40 of the digested n-6 and n-3 FA were deposited, respectively. Approximately one-third of the n-3 supply that was deposited resulted from the conversion of 18 : 3 to other metabolites (i.e. EPA, docosapentaenoic acid and DHA). This proportion was affected by the pig genotype. De novo-synthesised FA represented 0·86 of the total non-essential FA deposition, and its average composition corresponded to 0·017, 0·286, 0·025, 0·217 and 0·454 for 14 : 0, 16 : 0, 16 : 1, 18 : 0 and 18 : 1, respectively. Although the average whole-body FA composition was relatively constant during the finishing period, this was not so for the tissues. In the carcass (without backfat), the content of 18 : 1 increased during the finishing period, whereas that of 16 : 0 and 18 : 0 decreased. Backfat captured a proportionally greater fraction of 18 : 2 than did the carcass or the residual tissues. In contrast, a proportionally greater fraction of the dietary 18 : 3 supply was deposited in the carcass compared to other tissues.

Differential Incorporation of Dietary Fatty Acids from Flax and Fish Oils into Lipid Classes of White Bass Ova

2011 ◽  
Vol 73 (2) ◽  
pp. 212-220 ◽  
Author(s):  
Heidi A. Lewis ◽  
Jesse T. Trushenski ◽  
Ryan L. Lane ◽  
Christopher C. Kohler
Keyword(s):  
Fatty Acids ◽  
Dietary Fatty Acids ◽  
Lipid Classes ◽  
Fish Oils ◽  
White Bass

scholarly journals Lipid-dependent Bidirectional Traffic of Apolipoprotein B in Polarized Enterocytes

2004 ◽  
Vol 15 (1) ◽  
pp. 132-141 ◽  
Author(s):  
Etienne Morel ◽  
Sylvie Demignot ◽  
Danielle Chateau ◽  
Jean Chambaz ◽  
Monique Rousset ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Apolipoprotein B ◽  
Secretory Protein ◽  
Dietary Fatty Acids ◽  
Transepithelial Transport ◽  
Intracellular Traffic ◽  
Polarized Cells ◽  
Complex Lipid ◽  
Insight Into ◽  
Lipid Micelles

Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by lipids is therefore of particular interest. To get a dynamic insight into this process, we used the enterocytic Caco-2 cells cultured on microporous filters, a system in which the apical and basal compartments can be delimited. Combining biochemical and morphological approaches, our results showed that, besides their role in protection from degradation, lipids control the intracellular traffic of apoB in enterocytes. A supply of fatty acids and cholesterol is sufficient for the export of apoB from the endoplasmic reticulum and its post-Golgi traffic up to the apical brush-border domain, where it remains until an apical supply of complex lipid micelles signals its chase down to the basolateral secretory domain. This downward traffic of apoB involves a microtubule-dependent process. Our results demonstrate an enterocyte-specific bidirectional process for the lipid-dependent traffic of a secretory protein.

Postprandial Activation of Coagulant Factor VII by Long-chain Dietary Fatty Acids

1996 ◽  
Vol 76 (03) ◽  
pp. 369-371 ◽  
Author(s):  
T A B Sanders ◽  
G J Miller ◽  
Tamara de Grassi ◽  
Najat Yahia
Keyword(s):  
Fatty Acids ◽  
Dietary Fatty Acids ◽  
Factor Vii ◽  
Fat Intake ◽  
Long Chain Fatty Acids ◽  
Postprandial Lipaemia ◽  
Increased Risk ◽  
Coagulant Activity ◽  
Long Chain Triglycerides ◽  
Long Chain

SummaryFactor VII coagulant activity (FVIIc) is associated with an increased risk of fatal ischaemic heart disease (IHD). Several reports have suggested that dietary fat intake or hypertriglyceridaemia are associated with elevated levels of FVII. This study demonstrates that an intake of long-chain fatty acids sufficient to induce postprandial lipaemia in healthy subjects leads to a substantial elevation in both FVIIc and the concentration of FVII circulating in the activated form. Such an increase in FVIIc could not be induced by medium-chain triglycerides. These results suggest that the consumption of a sufficient amount of long-chain triglycerides to induce postprandial lipaemia induces the activation of FVII.

Differential Effects of Dietary Fatty Acids on Body Composition and Adiposity

2020 ◽  
Vol 16 (2) ◽  
pp. 142-154 ◽  
Author(s):  
Hadi Emamat ◽  
Zahra Yari ◽  
Hossein Farhadnejad ◽  
Parvin Mirmiran
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Total Body Weight ◽  
Fat Distribution ◽  
Monounsaturated Fatty Acids ◽  
Dietary Fatty Acids ◽  
Dietary Fats ◽  
Total Body

Recent evidence has highlighted that fat accumulation, particularly abdominal fat distribution, is strongly associated with metabolic disturbance. It is also well-recognized that the metabolic responses to variations in macronutrients intake can affect body composition. Previous studies suggest that the quality of dietary fats can be considered as the main determinant of body-fat deposition, fat distribution, and body composition without altering the total body weight; however, the effects of dietary fats on body composition have controversial results. There is substantial evidence to suggest that saturated fatty acids are more obesogen than unsaturated fatty acids, and with the exception of some isomers like conjugate linoleic acid, most dietary trans fatty acids are adiposity enhancers, but there is no consensus on it yet. On the other hand, there is little evidence to indicate that higher intake of the n-3 and the n-6 polyunsaturated fatty acids can be beneficial in attenuating adiposity, and the effect of monounsaturated fatty acids on body composition is contradictory. Accordingly, the content of this review summarizes the current body of knowledge on the potential effects of the different types of dietary fatty acids on body composition and adiposity. It also refers to the putative mechanisms underlying this association and reflects on the controversy of this topic.

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