scholarly journals Lipidomics Analysis of the Tears in the Patients Receiving LASIK, FS-LASIK, or SBK Surgery

2021 ◽  
Vol 8 ◽  
Author(s):  
Yan Gao ◽  
Yuanyuan Qi ◽  
Yue Huang ◽  
Xiaorong Li ◽  
Lei Zhou ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Statistical Significance ◽  
Direct Injection ◽  
Tear Film ◽  
Vital Role ◽  
Shotgun Lipidomics ◽  
Film Stability ◽  
Minimal Impact ◽  
Significant Difference ◽  
Over Time

Purpose: Tear film lipid layer (TFLL) plays a vital role in maintaining the tear film stability and, thus, the lipid composition of the tears could greatly affect the physiological function and biophysical integrity of the tear film. The objective of this study is to assess the tear lipid composition of the patients receiving laser-assisted in situ keratomileusis (LASIK), femtosecond LASIK (FS-LASIK), or sub-Bowman's keratomileusis (SBK) surgery preoperatively and postoperatively.Methods: Tear samples were collected from the left eye of the patient who receiving LASIK (n = 10), FS-LASIK (n = 10), or SBK (n = 10) surgery in week 0, week 1, week 4, and week 52. A rapid direct injection shotgun lipidomics workflow, MS/MSALL (<2 min/sample), was applied to examine the tear lipidome.Results: In week 52, the SBK group demonstrated a similar lipidome profile compared to week 0, while the FS-LASIK and LASIK groups shifted away from week 0. Two lipids, ganglioside (GD3) 27:4 and triacylglycerol (TAG) 59:3, were found to be associated with the lipidome changes preoperatively and postoperatively. No statistical significance was found in the overall lipid classes from the FS-LASIK group. The LASIK group showed significant alteration in the phospholipid and sphingolipid over time, while the SBK group demonstrated a significant difference in the (O-acyl)-ω-hydroxy fatty acid (OAHFA) and phospholipid.Conclusion: LASIK showed the greatest impact on the tear lipidome changes over time, while SBK demonstrated minimal impact among the three types of refractive surgeries after 1 year.

scholarly journals A Controlled Study on the Correlation between Tear Film Volume and Tear Film Stability in Diabetic Patients

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Iman M. Eissa ◽  
Noha M. Khalil ◽  
Heba A. El-Gendy
Keyword(s):  
Anterior Segment ◽  
Tear Film ◽  
Controlled Study ◽  
Control Group ◽  
Diabetic Patients ◽  
Film Stability ◽  
Duration Of Diabetes ◽  
Significant Difference ◽  
Tear Meniscus ◽  
Tear Meniscus Height

Purpose. To assess the tear film quantity and correlate it with the quality and stability of the tear film in diabetics and compare them to age matched controls.Introduction. Diabetes affects tear film parameters in multiple ways. Poor metabolic control and neuropathy are postulated factors. To further understand how diabetes affects tear film parameters this study was conducted.Subjects and Methods. Tear meniscus height was measured by anterior segment OCT, along with tear thinning time, a subtype of noninvasive tear break-up time, and blinking rate per minute which were all recorded for 22 diabetic patients. Correlations between these tear film parameters were studied and then compared to 16 age matched controls.Results. A statistically significant difference was found in blinking rate between the diabetic and the control group (P=0.002), with higher blinking rate among diabetics. All tear film parameters were negatively correlated with duration of diabetes. A positive correlation was found between tear film volume and stability.Conclusion. Diabetes affects the tear film in various ways. Diabetics should be examined for dry eye signs even in absence of symptoms which may be masked by associated neuropathy. Duration of diabetes has an impact on tear film status.

scholarly journals The impact of pseudoexfoliation and arteficial tear application on tear film stability in a pseudophakic eye

2018 ◽  
Vol 146 (7-8) ◽  
pp. 422-427
Author(s):  
Dusan Todorovic ◽  
Tatjana Sarenac-Vulovic ◽  
Svetlana Jovanovic ◽  
Mirjana Janicijevic-Petrovic ◽  
Nenad Petrovic ◽  
...  
Keyword(s):  
Cataract Surgery ◽  
Dry Eye ◽  
Statistical Significance ◽  
Tear Film ◽  
Ocular Surface Disease Index ◽  
Film Stability ◽  
Group Iii ◽  
Group Ii ◽  
One Year ◽  
The Impact

Introduction/Objective. Dry eye development after phacoemulsification is a very common complication of cataract surgery. Combined with other risk factors, such as pseudoexfoliation (PEX), this complication becomes more frequent. The aim of this study was to expose the influence of phacoemulsification associated with PEX on the development of dry eye in patients who underwent cataract surgery. Methods. The study included 160 eyes (160 patients) who underwent phacoemulsification. The patients were divided into four groups (n = 40 patients). Groups I and II involved patients without PEX. The patients from Group II were prescribed artificial tears (AT) postoperatively. The patients from Groups III and IV had a presentation of PEX. The patients from Group IV got AT postoperatively. Ocular Surface Disease Index, the tear breakup time (TBUT) test, fluorescein staining, and Schirmer?s test served as ?dry eye? tests in this study. The ?2 test and paired t-test were used for data analysis. Results. Measurements were taken for every patient preoperatively, on the first and the seventh day after the surgery, one month and three months, and one year after the surgery. The tests showed postoperatively developed dry eye in all the groups, with the recovery of the tear film within three months, except for Group III. The highest statistical significance (p < 0.001) was recorded between Groups II and III, three and 12 months after the surgery. Values of TBUT (6.09 ? 1.29 seconds) and Schirmer?s test (8.91 ? 2.14 mm) in Group III strongly indicated that dry eye was still present one year after phacoemulsification. The greatest recovery of the tear film was recorded in Group II in all the measurements. Conclusion. The study showed how strongly PEX and the use of AT affect the tear film during the first postoperative year. Based on our experience, we highly recommend the use of AT after performing phacoemulsification.

scholarly journals Lipid Conformational Order and the Etiology of Cataract and Dry Eye

2020 ◽  
pp. jlr.TR120000874 ◽  
Author(s):  
Douglas Borchman
Keyword(s):  
Dry Eye ◽  
Lipid Composition ◽  
Tear Film ◽  
The Body ◽  
Molar Ratio ◽  
Human Lens ◽  
Film Stability ◽  
Composition And Structure ◽  
Conformational Order ◽  
And Function

     Lens and tear film lipids are as unique as the systems they reside in. The major lipid of the human lens is dihydrosphingomylein, found in quantity only in the lens. The lens contains a cholesterol to phospholipid molar ratio as high as 10:1, more than anywhere in the body. Lens lipids contribute to maintaining lens clarity, and alterations in lens lipid composition due to age are likely to contribute to cataract. Lens lipid composition reflects adaptations to the unique characteristics of the lens: no turnover of lens lipids or proteins; the lowest amount of oxygen than any other tissue and contains almost no intracellular organelles. The tear film lipid layer (TFLL) is also unique. The TFLL is a thin, 100 nm layer of lipid on the surface of tears covering the cornea that contributes to tear film stability. The major lipids of the TFLL are wax esters and cholesterol esters that are not found in the lens. The hydrocarbon chains associated with the esters are longer than those found anywhere in the body, as long as 32 carbons, and many are branched. Changes in the composition and structure of the 30,000 different moieties of TFLL contribute to the instability of tears. The focus of the current review is how spectroscopy has been used to elucidate the relationships between lipid composition, conformational order and function and the etiology of cataract and dry eye.

scholarly journals Perioperative antibiotic prophylaxis: tools, results and reflections from a 6-year monitoring

2019 ◽  
Vol 29 (Supplement_4) ◽  
Author(s):  
M d’Angelo ◽  
F Malacarne ◽  
G Perri ◽  
C Smaniotto ◽  
C Battistella ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Antimicrobial Prophylaxis ◽  
Linear Trend ◽  
Statistical Significance ◽  
Linear Regression Analysis ◽  
Intervention Strategies ◽  
Significant Difference ◽  
Clinical Records ◽  
Over Time

Abstract Background Perioperative antimicrobial prophylaxis is a key practice to prevent surgical site infection. The Udine Healthcare and University Integrated Trust has defined its features in a specific procedure, available on the hospital Intranet. Compliance with the procedure has been monitored on a semi-annual basis. Methods Between July 2013 and December 2018 15 clinical records per semester for each one of the 16 surgical units were randomly extracted, including ordinary admissions with clean or clean/contaminated surgery. Compliance with the procedure was monitored for all the items considered: drug, dosage, timing of administration, redosing and duration of prophylaxis. A first comparison analysis both between semesters and for longer time frames was performed with the chi-square test; to evaluate the presence of a linear trend all over time (in semestres), the Pearson linear correlation coefficient was calculated; in case of correlation, a simple two-variable linear regression analysis was performed. Statistical significance was assessed for p &lt; 0.05. Results A sample of 2775 records was evaluated. Focusing on the first and last semester of monitoring, overall compliance increased in 9 of the 16 units, although there is a drop, when extending the analysis to the whole sample (72% vs 70.8%) with no statistical significance. Otherwise a statistically significant difference is recorded between the first and the last two years of analysis (p &lt;0.007), with an increase of overall compliance from 64.2% (2013-2014) to 69.7% (2017-2018). Finally a strong and significant negative correlation between time and overall compliance to redosing emerges (r = -0.79; p = 0.004). Conclusions Compliance with hospital procedure has substantially improved over time reaching a stable performance value. However the linear regression analysis highlights the need to implement new intervention strategies in order to increase overall compliance to redosing. Key messages Overall compliance with hospital procedure has improved over time. Results highlight the need to implement new intervention strategies.

scholarly journals Short Term Effect of Virtual Reality on Tear Film Stability and Ocular Discomfort

2020 ◽  
Vol 4 (1) ◽  
pp. 40-46
Author(s):  
Mohd Zulfaezal Che Azemin ◽  
Keyword(s):  
Virtual Reality ◽  
Tear Film ◽  
Film Stability ◽  
The Public ◽  
Non Invasive ◽  
Significant Difference ◽  
Ocular Discomfort ◽  
Tear Film Stability ◽  
Before And After ◽  
Tear Meniscus Height

Virtual reality (VR) has been integrated and used with smartphones as one of the digital entertainments such as in gaming and movie streaming. With emergent of various VR brands in the market, it concerns the public on the possible side effects of VR on the ocular performance specifically on tear film stability and ocular discomfort. The purpose of this study was to compare the change on non-invasive keratograph tear break-up time (NIKBUT), tear meniscus height (TMH) before and after the use of VR for 30 minutes, and to measure the ocular discomfort after the use of VR quantitatively. Thirty-two subjects were recruited in this study and all the subjects were divided randomly into two groups; VR and laptop (used as a control) groups. Each subject needed to watch a movie for 30 minutes using the respective devices. The changes of tear film stability and ocular discomfort before and after the use of the devices were then compared and the results showed that there was no significant difference (p>0.05) after 30 minutes of the use. Comparison of the ocular parameters between the devices on post-watching activities was also not significant (p>0.05). Use of VR did not give any signs of dryness to the eyes and it can be used without any discomfort even after 30 minutes usage of the devices.

Emerging value of multigene panels for germline testing in patients with neuroendocrine tumors.

2018 ◽  
Vol 36 (4_suppl) ◽  
pp. 226-226
Author(s):  
Julia Whitman ◽  
Brandon Shih ◽  
Amie Blanco ◽  
Salina Chan ◽  
Alan Paciorek ◽  
...  
Keyword(s):  
Neuroendocrine Tumors ◽  
Cancer Genetics ◽  
Statistical Significance ◽  
Single Gene ◽  
Logistic Models ◽  
Germline Mutations ◽  
Significant Difference ◽  
Multigene Panels ◽  
Over Time ◽  
Germline Testing

226 Background: Neuroendocrine tumors (NETs) are known to be associated with hereditary syndromes stemming from MEN1, VHL, SDH or TSC mutations. Recent data suggest that additional germline mutations may be relevant, implying a role of germline testing with multigene panels. We examined genetic counseling (GC) referral and testing patterns, test results, and their changes over time in NET patients (pts). Methods: Retrospective chart review was conducted in 236 NET pts referred to UCSF Cancer Genetics and Prevention Program 2004-2017. Univariate logistic models were used to assess relationship between binary outcome and covariate. STATA was used for analysis and statistical significance was based on p < 0.05. Results: 139 referred pts (59%) followed up with GC. Pts with >1 family members diagnosed with cancer were more likely to attend GC [OR=2.75, p=0.010]. Among 107 pts tested, small bowel NETs were less associated with testing than pancreatic NETs [OR=0.15, p=0.001]. Single-gene tests were routine until 2015, when panels up to 130 genes became standard. Overall, 31 pts (29% of 107 tested) had a pathogenic/likely pathogenic (P/LP) result. There was no significant difference between single and multi-gene tests in identifying P/LP mutations (likely due to changes in threshold for testing over time), but greater diversity in P/LP mutations was noted with larger panels. Functional tumors showed lower rate of P/LP mutations than non-functional [OR=0.17, p=0.037]. Conclusions: Only 59% of referred pts followed up with GC, suggesting significant barriers to testing exist. Of those tested, 29% harbored a P/LP mutation. Germline mutations not traditionally associated with NETs were identified, highlighting the potential importance of larger panels to detect rare mutations. [Table: see text]

scholarly journals An easy and effective test to evaluate tear film stability as a self diagnosis of dry eye syndrome: Blinking tolerance time (BTT)

2020 ◽  
Author(s):  
Hyung Bin Hwang ◽  
Yong Ho Ku ◽  
Eun Chul Kim ◽  
Hyun Seung Kim ◽  
Man Soo Kim ◽  
...  
Keyword(s):  
Dry Eye ◽  
Intraclass Correlation ◽  
Tear Film ◽  
Controlled Study ◽  
Cutoff Score ◽  
Time Interval ◽  
Film Stability ◽  
Tolerance Time ◽  
Significant Difference ◽  
Tear Film Stability

Abstract Background: To introduce a new diagnostic test, blinking tolerance time (BTT) to evaluate tear film stability. We will compare it with tear break-up time (tBUT) and validate the value of the BTT as a new self-diagnostic tool for assessing tear film instability.Methods: This was a prospective controlled study involving 212 eyes of 106 participants aged 20–79 years. One hundred fourteen eyes of 57 dry eye patients and 98 eyes of 49 healthy subjects were included in the study. All patients and subjects were administered the following diagnostic tests of dry eye disease: Ocular Surface Disease Index, BTT, tBUT, slit lamp examination, corneal stain score, and Schirmer I test (without anesthesia). Patients and subjects were instructed to not to blinking as possible as he or she can after reset blinking. Examiner measure the time interval between reset blinking and next blinking. tBUTou was defined as the mean of the average of three tBUT values in the right and left eyes. Correlations between BTT and tBUTou were also evaluated. To evaluate the diagnostic efficacy of the BTT and tBUT, ROC curves were generated to obtain a cutoff score, and the sensitivities of the tests against the specificity at all possible thresholds were plotted.Results: Spearman’s correlation analysis showed a statistically significant, weakly positive correlation between BTT and tBUTOU (r = 0.447; p=0.000). The intraclass correlation coefficient (ICC) of the tBUT was 0.679 (95% confidence interval [CI]: 0.575–0.765) and the ICC of the BTT was 0.904 (95% CI: 0.867–0.932). The AUC of the tBUTOU was 0.678 and that of the BTT was 0.628. There was no statistically significant difference between these two AUC values (p=0.641). When the cutoff value of BTT was set to 8.1 sec, the sensitivity of the BTT was 63.3% and the specificity was 56.1%.Conclusion: As a simple and new self-diagnostic diagnosis of dry eye, BTT has diagnostic value in clinical practice. The BTT test has a unique advantage in that it can be used for self-diagnostic diagnosis of dry eye. The BTT test can also be widely used in the clinical setting because it is an inexpensive and simple procedure.

Service Quality Perception towards E-CRM Practices among Customers of Private and Public Sector Banks of Rajasthan Using SERVQUAL Method

2018 ◽  
Vol 7 (3) ◽  
pp. 22
Author(s):  
Neeti Kasliwal ◽  
Jagriti Singh
Keyword(s):  
Public Sector ◽  
Service Quality ◽  
Banking Sector ◽  
Sampling Technique ◽  
Vital Role ◽  
Public Sector Banks ◽  
Significant Difference ◽  
Private And Public ◽  
Score Method ◽  
Public Banks

Banking sector is growing rapidly and playing a vital role in the economic development of the nation. Both private and public sector banks are giving more priority to service quality to satisfy their customers. For this, banks are now emphasizing on E-CRM practices to carry out transactions and communicate with their customers. The purpose of this research is to assess the service quality among private and public banks in Rajasthan. Purposive sampling technique has been employed to collect the data from three private banks and three banks from public. To analyze the data, descriptive statistics, Mean score method and t test have been used. Results indicates that there is a significant difference in consumer’s perception of service quality dimensions related to E-CRM practices provided by selected private and public sector banks of Rajasthan..The findings of this research will help policy makers of banking sector to set customer oriented policies.

Comparative study of Clinical Characteristics in Patients with Mild and Severe Reflux Esophagitis

2020 ◽  
Vol 66 (1) ◽  
pp. 19-22
Author(s):  
Melania Macarie ◽  
Simona Maria Bataga ◽  
Monica Pantea ◽  
Razvan Opaschi ◽  
Simona Mocan ◽  
...  
Keyword(s):  
Risk Factors ◽  
Sex Ratio ◽  
Hiatal Hernia ◽  
Statistical Significance ◽  
Erosive Esophagitis ◽  
Male Gender ◽  
Consecutive Series ◽  
Study Group ◽  
Entire Study ◽  
Significant Difference

AbstractObjective: This study aims to determine the correlation between risk factors and erosive esophagitis development.Methods: We conducted a retrospective observational study on a consecutive series of 19.672 patients who underwent upper gastrointestinal endoscopy between 01.01.2011-31.12.2017. A total of 3005 patients, diagnosed with erosive esophagitis, were included in the present study and stratified according to Los Angeles classification.Results: During the studied period we found 3005 patients with erosive esophagitis, sex ratio male to female was 1.3/1, the most common forms of esophagitis being grade A and B: 74.54% patients with esophagitis grade A, 14.80% patients with grade B; 5.29% patients were with grade C and 5.35% patients with esophagitis grade D. In severe esophagitis the male predominance was more prevalent (249 males, 71 female), with a sex ratio 3.50/1. The correlation of male gender with severe esophagitis was highly statistically significant (p < 0.0001, OR 2.97; 95% CI 2.25-3.91). Hiatal hernia was diagnosed in 1171 patients, the presence of large hiatal hernias, being an important predictor, with statistical significance (p < 0.0001, OR 3.41; 95% CI 2.22-5.21), for severe esophagitis development. Incidence of Helicobacter pylori infection was 11.51%, in the entire study group, with no statistical significant difference between patients with mild or severe esophagitis (12.02% vs 7.18%).Conclusion: Erosive esophagitis is a frequent disease, the most common forms being grade A and B. Male gender and the presence of hiatal hernia are the most important risk factors for erosive esophagitis development, in our study group.

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