scholarly journals Optimization of Growth Conditions to Produce Sustainable Polyhydroxyalkanoate Bioplastic by Pseudomonas aeruginosa EO1

2021 ◽  
Vol 12 ◽  
Author(s):  
Richa Prasad Mahato ◽  
Saurabh Kumar ◽  
Padma Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Edible Oils ◽  
Saturated Fatty Acids ◽  
Growth Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Mustard Oil ◽  
Oilseed Crop ◽  
Groundnut Oil ◽  
Dry Cell Weight ◽  
Pha Production

Polyhydroxyalkanoates (PHAs) are intracellularly synthesized by bacteria as carbonosomes that exhibit biodegradable thermoplastics and elastomeric properties. The use of cheaper edible oils as a source of carbon assists in the reduction of the production cost of such biopolyesters. In this work, different edible oils, such as groundnut oil (GNO), mustard oil, sesame oil, and soybean oil (SBO) were used to check their effect on PHA production from Pseudomonas aeruginosa EO1 (MK049902). Pseudomonas aeruginosa EO1 was used in a two-stage production system. In the first stage, bacterial growth was favored and, in the second, PHA was synthesized. GNO was found as the best carbon source for PHA production. The use of 2% (v/v) GNO, rich in saturated fatty acids, allowed PHA content of 58.41% and dry cell weight (DCW) of 10.5g/L at pH7 and temperature 35°C for 72h. Groundnut has a high potential for oil production and for the diversification of co-products with some potential of value aggregation. Such a perennial and sustainable species will almost certainly meet the criteria for becoming a significant commercial oilseed crop. Fourier transform infrared spectroscopy (FTIR) spectra showed strong characteristic bands at 1,282, 1,725, 2,935, 2,999, and 3,137cm−1 for the PHA polymer. Gas chromatography-mass spectrometry (GC-MS) detects the presence of PHA copolymers.

scholarly journals Autotrophic and Heterotrophic Growth Conditions Modify Biomolecole Production in the Microalga Galdieria sulphuraria (Cyanidiophyceae, Rhodophyta)

Marine Drugs ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 169
Author(s):  
Roberto Barone ◽  
Lorenzo De Napoli ◽  
Luciano Mayol ◽  
Marina Paolucci ◽  
Maria Grazia Volpe ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Principal Component ◽  
Monounsaturated Fatty Acids ◽  
Growth Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Galdieria Sulphuraria ◽  
Trophic Conditions ◽  
Carbon Mass

Algae have multiple similarities with fungi, with both belonging to the Thallophyte, a polyphyletic group of non-mobile organisms grouped together on the basis of similar characteristics, but not sharing a common ancestor. The main difference between algae and fungi is noted in their metabolism. In fact, although algae have chlorophyll-bearing thalloids and are autotrophic organisms, fungi lack chlorophyll and are heterotrophic, not able to synthesize their own nutrients. However, our studies have shown that the extremophilic microalga Galderia sulphuraria (GS) can also grow very well in heterotrophic conditions like fungi. This study was carried out using several approaches such as scanning electron microscope (SEM), gas chromatography/mass spectrometry (GC/MS), and infrared spectrophotometry (ATR-FTIR). Results showed that the GS, strain ACUF 064, cultured in autotrophic (AGS) and heterotrophic (HGS) conditions, produced different biomolecules. In particular, when grown in HGS, the algae (i) was 30% larger, with an increase in carbon mass that was 20% greater than AGS; (ii) produced higher quantities of stearic acid, oleic acid, monounsaturated fatty acids (MUFAs), and ergosterol; (iii) produced lower quantities of fatty acid methyl esters (FAMEs) such as methyl palmytate, and methyl linoleate, saturated fatty acids (SFAs), and poyliunsaturated fatty acids (PUFAs). ATR-FTIR and principal component analysis (PCA) statistical analysis confirmed that the macromolecular content of HGS was significantly different from AGS. The ability to produce different macromolecules by changing the trophic conditions may represent an interesting strategy to induce microalgae to produce different biomolecules that can find applications in several fields such as food, feed, nutraceutical, or energy production.

scholarly journals Quality and vitamin A status assessment of different commercial edible oil

2019 ◽  
Vol 54 (1) ◽  
pp. 11-20
Author(s):  
SA Lisa ◽  
S Khan ◽  
MA Kabir ◽  
F Islam ◽  
S Mohajan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Vitamin A ◽  
Rice Bran ◽  
Palm Olein ◽  
Edible Oils ◽  
Rice Bran Oil ◽  
Saturated Fatty Acids ◽  
Mustard Oil ◽  
Mustard Oils

The present study was done to evaluate the quality and the vitamin A fortification status of 5 types of commercial edible oils sold in Dhaka city markets. The percentages of free fatty acid (FFA), peroxide value (POV), iodine value (IV) and fatty acid composition were estimated to evaluate the quality and vitamin A content were determined to evaluate the vitamin A fortification status of 25 commercial edible oils- soybean oil, sunflower oil, rice bran oil, mustard oil and palm olein (5 brands of each) . The results revealed that FFA, POV and IV of the commercial oils analyzed were within the BSTI standard limits. The highest saturated fatty acids (SFA) values ranging from 39.85 ± 0.005 to 46.97 ± 0.002 % was found in palm olein samples and the lowest SFA values ranging from 4.56 ± 0.031 to 6.97 ± 0.096 % was found in mustard oils. On the other hand, mustard oils contained highest monounsaturated fatty acids (MUFA) ranging from 69.30 ± 0.189 to 75.87 ± 0.069 % compared to other oils analyzed. Highest polyunsaturated fatty acids (PUFA) were recorded in sunflower oils 64.78 ± 0.035% followed by soybean oils 63.25 ± 0.245%. In addition, except sunflower and mustard oils, vitamin A was found in all the soybean and rice bran oils analyzed. On the contrary, vitamin A was found only in 60% of the palm olein samples analyzed and the content was much lower than the standard value (15-30 ppm). Bangladesh J. Sci. Ind. Res.54(1), 11-20, 2019

Organochlorine Pesticide Residues in Different Indian Cereals, Pulses, Spices, Vegetables, Fruits, Milk, Butter, Deshi Ghee, and Edible Oils

1990 ◽  
Vol 73 (4) ◽  
pp. 509-512 ◽  
Author(s):  
Bhupendra S Kaphalia ◽  
Renu Takroo ◽  
Suman Mehrotra ◽  
Usha Nigam ◽  
Tejeshwar D Seth
Keyword(s):  
Organochlorine Pesticide ◽  
Wheat Flour ◽  
Pesticide Residues ◽  
Edible Oils ◽  
Black Pepper ◽  
Mustard Oil ◽  
Gas Liquid Chromatography ◽  
Groundnut Oil ◽  
Organochlorine Pesticide Residues ◽  
Vegetables And Fruits

Abstract A total of 244 samples of cereals (wheat flour, rice, and maize), pulses (arhar, moong, gram, lentil, and black gram), spices (turmeric, chili, coriander, and black pepper), vegetables (potato, onion, spinach, cabbage, brinjal, and tomato), fruits (mango, guava, apple, and grape), milk, butter, DeshI ghee, and edible oils (vegetable, mustard, groundnut, and sesame) collected from different cities of Northern Province (Utter Pradesh) were analyzed by gas liquid chromatography for the presence of organochlorine pesticide residues. Residues of hexachlorocyclohexane (HCH) and 2,2-bls(p-chlorophenyl)- 1,1,1-trlchloroethane (DDT) were detected In about 85% of the total samples of cereals, spices, milk, butter, DeshI ghee, and edible oils analyzed in the present study. However, the residue levels were either very small (<0.06 ppm) or not detected at all In pulses, vegetables, and fruits as compared with very high concentrations In wheat flour (4.42 and 0.12 ppm), butter (1.19 and 4.85 ppm), mustard oil (1.26 and 2.42 ppm), DeshI ghee (1.10 and 3.84 ppm), vegetable oil (1.02 and 0.59 ppm), groundnut oil (0.51 and 1.49 ppm), and chili (0.48 and 1.92 ppm). The levels of HCH and DDT residues detected in rice, maize, turmeric, coriander, black pepper, and all the vegetables and fruits were also lower than those found In wheat flour, oil, and fat samples analyzed In the present study. These findings suggest that a restricted and controlled use of such persistent pesticides may be useful for decreasing their contamination levels In different food Items.

Association between mustard oil consumption and BMI in India

2020 ◽  
pp. 1-9
Author(s):  
Rutuja Chhajed ◽  
Tinku Thomas ◽  
Sumathi Swaminathan ◽  
Anura V Kurpad ◽  
Indu Mani
Keyword(s):  
Weight Status ◽  
Edible Oils ◽  
National Level ◽  
Sample Survey ◽  
Mustard Oil ◽  
Adult Women ◽  
Inverse Association ◽  
Oil Consumption ◽  
Groundnut Oil ◽  
Nationally Representative

Abstract Objective: This study examined the association between predominant vegetable oil consumed and BMI of adult women and men in India, with emphasis on predominant consumption of mustard oil. Design: Two nationally representative data, the consumer expenditure survey of National Sample Survey Office (NSSO)-68th round (2011–2012) for food consumption and National Family Health Survey-4 (2015–2016) for weight status, were analysed. Data from both surveys were combined by matching households through common matching variables (‘family size’, ‘religion’, etc.) using Nearest-Neighbour Hot-Deck matching. The association of overweight/obesity with predominant mustard oil consumption in the household was examined using logistic regression adjusted for confounders. The NSSO reports household consumption of mustard, groundnut, coconut, refined oils (sunflower, safflower, soyabean) and all other edible oils. Setting: Two nationally representative surveys from India. Participants: Total of 638 445 women and 92 312 men, respectively. Results: Mustard oil was the most predominantly consumed oil (51 %) followed by refined oils (32·4 %). Prevalence of overweight/obesity in women and men was lower in households with predominant mustard oil consumption (17 and 15 %) v. all other refined and other oils combined (27 and 26 %). The adjusted OR for predominant mustard oil use was 0·88 (95 % CI 0·86, 0·87) for women and 0·80 (95 % CI 0·76, 0·83) for men. A similar analysis with predominant groundnut oil consumption showed no association with overweight/obesity. Conclusions: The data from a large national level survey suggest an inverse association between mustard oil consumption and overweight/obesity which needs to be explored with further research studies.

scholarly journals EFFECT OF FRYING TEMPERATURE/FRYING CYCLES ON trans-FATS AND OXIDATIVE STABILITY OF GROUNDNUT OIL - CARDIAC RISK FACTORS

2019 ◽  
Vol 12 (1) ◽  
pp. 452
Author(s):  
Akanksha Jain ◽  
Santosh Jain Passi ◽  
William Selvamurthy ◽  
Archna Singh
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Edible Oils ◽  
Saturated Fatty Acids ◽  
Acid Value ◽  
Cardiac Risk ◽  
Groundnut Oil ◽  
Total Oxidation ◽  
Oxidative Deterioration ◽  
Frying Process

Objective: Frying process leads to oxidative deterioration and generation of trans fatty acids (TFAs) in fats/oils resulting in elevated cardiac risk. The objective of this study was to assess the effect of frying temperatures and the number of frying cycles on TFA and primary/secondary oxidative indices of groundnut oil used for preparing French fries.Methods: TFA (by gas chromatography) and other chemical parameters (by American Oil Chemists’ Society official methods) were estimated at varying temperatures (160°C, 180°C, 200°C, 220°C and 230°C) and frying cycles (1st, 4th, 8th 16th and 32nd).Results: With soaring frying temperatures and progressive frying cycles, mean cis-unsaturated fatty acids decreased while mean saturated fatty acids and TFA increased. Temperature variation indicated increase in mean TFA from 0.13±0.02 g/100 g (160°C; 32nd cycle) to 1.88±0.04 g/100g (230°C;32nd cycle) (p<0.05). With successive frying cycles, TFA increased from 0.10±0.01 g/100 g (1st cycle) to 0.13±0.02 g/100 g (32nd cycle) when oil was heated to 160°C; and 1.16±0.02 g/100 g (1st cycle) to 1.88±0.04 g/100 g (32nd cycle) when oil reached 230°C. Acid value, p-anisidine value and total oxidation value demonstrated a significant increase at varying frying temperatures across the frying cycles (p<0.05); however, peroxide value (PV) indicated an inconsistent trend.Conclusion: TFA and oxidative parameters increased with elevating frying temperatures and progressive frying cycles; however, PV demonstrated inconsistency. Food safety agencies need to formulate policies, stringent food laws and impose necessary regulations to curb oil abuse during frying. There is a dire need to raise consumer awareness regarding deleterious health effects of TFA and oxidative deterioration of edible oils.

scholarly journals EFFECT OF FRYING TEMPERATURE/FRYING CYCLES ON trans-FATS AND OXIDATIVE STABILITY OF GROUNDNUT OIL - CARDIAC RISK FACTORS

2019 ◽  
Vol 12 (1) ◽  
pp. 452
Author(s):  
Akanksha Jain ◽  
Santosh Jain Passi ◽  
William Selvamurthy ◽  
Archna Singh
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Edible Oils ◽  
Saturated Fatty Acids ◽  
Acid Value ◽  
Cardiac Risk ◽  
Groundnut Oil ◽  
Total Oxidation ◽  
Oxidative Deterioration ◽  
Frying Process

Objective: Frying process leads to oxidative deterioration and generation of trans fatty acids (TFAs) in fats/oils resulting in elevated cardiac risk. The objective of this study was to assess the effect of frying temperatures and the number of frying cycles on TFA and primary/secondary oxidative indices of groundnut oil used for preparing French fries.Methods: TFA (by gas chromatography) and other chemical parameters (by American Oil Chemists’ Society official methods) were estimated at varying temperatures (160°C, 180°C, 200°C, 220°C and 230°C) and frying cycles (1st, 4th, 8th 16th and 32nd).Results: With soaring frying temperatures and progressive frying cycles, mean cis-unsaturated fatty acids decreased while mean saturated fatty acids and TFA increased. Temperature variation indicated increase in mean TFA from 0.13±0.02 g/100 g (160°C; 32nd cycle) to 1.88±0.04 g/100g (230°C;32nd cycle) (p<0.05). With successive frying cycles, TFA increased from 0.10±0.01 g/100 g (1st cycle) to 0.13±0.02 g/100 g (32nd cycle) when oil was heated to 160°C; and 1.16±0.02 g/100 g (1st cycle) to 1.88±0.04 g/100 g (32nd cycle) when oil reached 230°C. Acid value, p-anisidine value and total oxidation value demonstrated a significant increase at varying frying temperatures across the frying cycles (p<0.05); however, peroxide value (PV) indicated an inconsistent trend.Conclusion: TFA and oxidative parameters increased with elevating frying temperatures and progressive frying cycles; however, PV demonstrated inconsistency. Food safety agencies need to formulate policies, stringent food laws and impose necessary regulations to curb oil abuse during frying. There is a dire need to raise consumer awareness regarding deleterious health effects of TFA and oxidative deterioration of edible oils.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

scholarly journals Phosphorus stress induces the synthesis of novel glycolipids in Pseudomonas aeruginosa that confer protection against a last-resort antibiotic

2021 ◽  
Author(s):  
Rebekah A. Jones ◽  
Holly Shropshire ◽  
Caimeng Zhao ◽  
Andrew Murphy ◽  
Ian Lidbury ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Sensitivity ◽  
Polymyxin B ◽  
Growth Conditions ◽  
Membrane Lipid ◽  
Comparative Genomic ◽  
P Limitation ◽  
Lung Microbiome ◽  
Glycolipid Synthesis

AbstractPseudomonas aeruginosa is a nosocomial pathogen with a prevalence in immunocompromised individuals and is particularly abundant in the lung microbiome of cystic fibrosis patients. A clinically important adaptation for bacterial pathogens during infection is their ability to survive and proliferate under phosphorus-limited growth conditions. Here, we demonstrate that P. aeruginosa adapts to P-limitation by substituting membrane glycerophospholipids with sugar-containing glycolipids through a lipid renovation pathway involving a phospholipase and two glycosyltransferases. Combining bacterial genetics and multi-omics (proteomics, lipidomics and metatranscriptomic analyses), we show that the surrogate glycolipids monoglucosyldiacylglycerol and glucuronic acid-diacylglycerol are synthesised through the action of a new phospholipase (PA3219) and two glycosyltransferases (PA3218 and PA0842). Comparative genomic analyses revealed that this pathway is strictly conserved in all P. aeruginosa strains isolated from a range of clinical and environmental settings and actively expressed in the metatranscriptome of cystic fibrosis patients. Importantly, this phospholipid-to-glycolipid transition comes with significant ecophysiological consequence in terms of antibiotic sensitivity. Mutants defective in glycolipid synthesis survive poorly when challenged with polymyxin B, a last-resort antibiotic for treating multi-drug resistant P. aeruginosa. Thus, we demonstrate an intriguing link between adaptation to environmental stress (nutrient availability) and antibiotic resistance, mediated through membrane lipid renovation that is an important new facet in our understanding of the ecophysiology of this bacterium in the lung microbiome of cystic fibrosis patients.

Kinetics of medium-chain-length polyhydroxyalkanoate production by a novel isolate of Pseudomonas putida LS46

2012 ◽  
Vol 58 (8) ◽  
pp. 982-989 ◽  
Author(s):  
Parveen K. Sharma ◽  
Jilagamazhi Fu ◽  
Nazim Cicek ◽  
Richard Sparling ◽  
David B. Levin
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Saturated Fatty Acids ◽  
Dry Mass ◽  
Nucleotide Sequence Analysis ◽  
Glucose Medium ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Pha Production ◽  
Nitrogen Excess

Six bacteria that synthesize medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were isolated from sewage sludge and hog barn wash and identified as strains of Pseudomonas and Comamonas by 16S rDNA gene sequencing. One isolate, Pseudomonas putida LS46, showed good PHA production (22% of cell dry mass) in glucose medium, and it was selected for further studies. While it is closely related to other P. putida strains (F1, KT2440, BIRD-1, GB-1, S16, and W619), P. putida LS46 was genetically distinct from these other strains on the basis of nucleotide sequence analysis of the cpn60 gene hypervariable region. PHA production was detected as early as 12 h in both nitrogen-limited and nitrogen-excess conditions. The increase in PHA production after 48 h was higher in nitrogen-limited cultures than in nitrogen-excess cultures. Pseudomonas putida LS46 produced mcl-PHAs when cultured with glucose, glycerol, or C6–C14 saturated fatty acids as carbon sources, and mcl-PHAs accounted for 56% of the cell dry mass when cells were batch cultured in medium containing 20 mmol/L octanoate. Although 3-hydroxydecanoate was the major mcl-PHA monomer (58.1–68.8 mol%) in P. putida LS46 cultured in glucose medium, 3-hydroxyoctanoate was the major monomer produced in octanoate medium (88 mol%).

scholarly journals Genetic determinants of Pseudomonas aeruginosa biofilm establishment

Microbiology ◽  
2010 ◽  
Vol 156 (2) ◽  
pp. 431-441 ◽  
Author(s):  
Mathias Müsken ◽  
Stefano Di Fiore ◽  
Andreas Dötsch ◽  
Rainer Fischer ◽  
Susanne Häussler
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Laser Scanning ◽  
Growth Conditions ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Repair System ◽  
Ph Homeostasis ◽  
Genetic Determinants ◽  
Complex Process ◽  
Genes Encoding

The establishment of bacterial biofilms on surfaces is a complex process that requires various factors for each consecutive developmental step. Here we report the screening of the comprehensive Harvard Pseudomonas aeruginosa PA14 mutant library for mutants exhibiting an altered biofilm phenotype. We analysed the capability of all mutants to form biofilms at the bottom of a 96-well plate by the use of an automated confocal laser-scanning microscope and found 394 and 285 genetic determinants of reduced and enhanced biofilm production, respectively. Overall, 67 % of the identified mutants were affected within genes encoding hypothetical proteins, indicating that novel developmental pathways are likely to be dissected in the future. Nevertheless, a common theme that emerged from the analysis of the genes with a predicted function is that the establishment of a biofilm requires regulatory components that are involved in survival under microaerophilic growth conditions, arginine metabolism, alkyl-quinolone signalling, pH homeostasis and the DNA repair system.

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