scholarly journals The Attenuating Effect of Low-Intensity Pulsed Ultrasound on Hypoxia-Induced Rat Chondrocyte Damage in TMJ Osteoarthritis Based on TMT Labeling Quantitative Proteomic Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Sa Du ◽  
Chao Liang ◽  
Yujie Sun ◽  
Bowen Ma ◽  
Wenmo Gao ◽  
...  
Keyword(s):  
Treatment Group ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Network Analyses ◽  
Low Intensity ◽  
Hypoxic Conditions ◽  
Oxygen Treatment ◽  
Temporomandibular Joint Osteoarthritis

Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease with a complex and multifactorial etiology. An increased intrajoint pressure or weakened penetration can exacerbate the hypoxic state of the condylar cartilage microenvironment. Our group previously simulated the hypoxic environment of TMJOA in vitro. Low-intensity pulsed ultrasound (LIPUS) stimulation attenuates chondrocyte matrix degradation via a hypoxia-inducible factor (HIF) pathway-associated mechanism, but the mode of action of LIPUS is currently poorly understood. Moreover, most recent studies investigated the pathological mechanisms of osteoarthritis, but no biomarkers have been established for assessing the therapeutic effect of LIPUS on TMJOA with high specificity, which results in a lack of guidance regarding clinical application. Here, tandem mass tag (TMT)-based quantitative proteomic technology was used to comprehensively screen the molecular targets and pathways affected by the action of LIPUS on chondrocytes under hypoxic conditions. A bioinformatic analysis identified 902 and 131 differentially expressed proteins (DEPs) in the <1% oxygen treatment group compared with the control group and in the <1% oxygen + LIPUS stimulation group compared with the <1% oxygen treatment group, respectively. The DEPs were analyzed by gene ontology (GO), KEGG pathway and protein-protein interaction (PPI) network analyses. By acting on extracellular matrix (ECM)-associated proteins, LIPUS increases energy production and activates the FAK signaling pathway to regulate cell biological behaviors. DEPs of interest were selected to verify the reliability of the proteomic results. In addition, this experiment demonstrated that LIPUS could upregulate chondrogenic factors (such as Sox9, Collagen Ⅱ and Aggrecan) and increase the mucin sulfate content. Moreover, LIPUS reduced the hydrolytic degradation of the ECM by decreasing the MMP3/TIMP1 ratio and vascularization by downregulating VEGF. Interestingly, LIPUS improved the migration ability of chondrocytes. In summary, LIPUS can regulate complex biological processes in chondrocytes under hypoxic conditions and alter the expression of many functional proteins, which results in reductions in hypoxia-induced chondrocyte damage. ECM proteins such as thrombospondin4, thrombospondin1, IL1RL1, and tissue inhibitors of metalloproteinase 1 play a central role and can be used as specific biomarkers determining the efficacy of LIPUS and viable clinical therapeutic targets of TMJOA.

scholarly journals Histological and Radiological Evaluation of Low-Intensity Pulsed Ultrasound Versus Whole Body Vibration on Healing of Mandibular Bone Defects in Rats

Medicina ◽  
2020 ◽  
Vol 56 (9) ◽  
pp. 457
Author(s):  
Milad Etemadi Sh ◽  
Nan-Chen Hsieh ◽  
Seyed Shahin Movahed Mohammadi ◽  
Shahrooz Momeni ◽  
Seyed Mohammad Razavi ◽  
...  
Keyword(s):  
Bone Density ◽  
Whole Body Vibration ◽  
Bone Defects ◽  
Whole Body ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Mandibular Bone ◽  
Body Vibration ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Background and Objectives: Mechanical stimulation can improve the structural properties of the fracture site and induce the differentiation of different cell types for bone regeneration. This study aimed to compare the effect of low-intensity pulsed ultrasound stimulation (LIPUS) versus whole body vibration (WBV) on healing of mandibular bone defects. Materials and Methods: A mandibular defect was created in 66 rats. The rats were randomly divided into two groups of rats. Each group was subdivided randomly by three groups (n = 11) as follows: (I) control group, (II) treatment with LIPUS, and (III) treatment with WBV. The radiographic changes in bone density, the ratio of lamellar bone to the entire bone volume, the ratio of the newly formed bone to the connective tissue and inflammation grade were evaluated after 1 and 2 months. Results: LIPUS significantly increased the radiographic bone density change compared to the control group at the first and second month postoperatively (p < 0.01). WBV only significantly increased the bone density compared to the control group at the second month after the surgery (p < 0.01). Conclusions: Application of LIPUS and WBV may enhance the regeneration of mandibular bone defects in rats. Although LIPUS and WBV are effective in mandibular bone healing, the effects of LIPUS are faster and greater than WBV.

The Effect of Low Intensity Pulsed Ultrasound on Proliferation of Rat Condylar Cartilage Cells in Hypoxia Culture

2019 ◽  
Vol 9 (5) ◽  
pp. 630-636
Author(s):  
Qiong Li ◽  
Wei Geng ◽  
Jun Li ◽  
Chao Liang ◽  
Chaolumen Bao
Keyword(s):  
Treatment Effect ◽  
Temporomandibular Disorder ◽  
Mrna Level ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Improvement Effect ◽  
Cartilage Cells ◽  
Subchondral Bone Tissue

Temporomandibular disorder (TMD) is a common and frequently-occurring oral and maxillofacial disease. Restoration of the joint's cartilage and subchondral bone tissue is the key to treat TMD. Low intensity pulsed ultrasound (LIPUS) has been proved to be effective in facilitating chondrocyte's proliferation, which inspired us to probe its treatment effect on TMD. With an isolated and hypoxic cultured mandibular condylar chondrocytes (MCC), we established a model for TMD. HIF-1α and VEGF's mRNA level was increased in hypoxic cultured MCC, which indicates our model simulated the cell's status caused by TMD pathology. When stimulated with LIIPUS at 45 mW/cm2, the MCC's apoptosis rate was inhibited and its proliferation was improved with best efficiency. Further experiment revealed that LIPUS restored MCC's Metalloproteinase's (MMP) up-regulation and collagen II's downregulation in hypoxic cultured MCC, respectively, which might be related to LIPUS's improvement effect on MCC.

scholarly journals Effects of low intensity pulsed ultrasound to reduce the effusion volumes and pain with knee osteoarthritis: a randomized controlled trial

2018 ◽  
Vol 5 (1) ◽  
pp. 77
Author(s):  
P. Shanmuga Raju ◽  
Chokkarapu Ramu ◽  
N. S. Harshavardhan ◽  
K. Rajender ◽  
G. Sachin
Keyword(s):  
Randomized Controlled Trial ◽  
Knee Osteoarthritis ◽  
Controlled Trial ◽  
Control Group ◽  
Ultrasound Therapy ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Randomized Controlled ◽  
And Control

<p class="abstract"><strong>Background:</strong> The study aim was to determine the effect of low intensity pulsed ultrasound therapy to reduce the effusion volumes and pain in patients with Knee OA.</p><p class="abstract"><strong>Methods:</strong> This study design was randomized controlled trial. Total 50 patients diagnosed with Knee osteoarthritis were randomly assigned to two groups. Group I was using treatment of low intensity pulsed ultrasound therapy and group II was administered TENS with home exercise respectively. Treatments were 6 days per week and duration of 2 week. The amount of effusion volume will be measured via ultrasonograpy in knee.<strong></strong></p><p class="abstract"><strong>Results:</strong> The<strong> </strong>maximum number of cases are lying in age group &gt;60 years which is 28% and 40% in cases and control group respectively and age distribution in both the group is statistically not significant. The mean age of patients in cases and control group is 57.08±7.40 years and 58.04±9.93 years respectively.</p><p class="abstract"><strong>Conclusions:</strong> Low intensity pulsed ultrasound therapy significantly reduced the effusion volumes and pain in patients with knee osteoarthritis.</p>

scholarly journals Evaluation of histological changes in peri-implant bone tissue after ultrasound application at early healing stages

2018 ◽  
pp. 38-42 ◽  
Author(s):  
S.P. Rubnikovich ◽  
I.S. Khomich
Keyword(s):  
Bone Tissue ◽  
Dental Implants ◽  
Morphological Changes ◽  
Experimental Studies ◽  
Low Frequency ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Dental Implantation

The aim was to study the nature of the morphological changes over time in the bone tissue around the implants under the influence of low-intensity pulsed ultrasound in the experiment. Materials and methods. Experimental studies were carried out on rabbits of the chinchilla breed, with dental implants installed in the tibia – two experimental groups with ultrasound and one control group. The animals were withdrawn from the experiment at 1, 2, 4 and 8 weeks and histological examination of the sections of peri-implant tissues was carried out. Results. The processes of osseointegration of dental implants in all groups of animals occurred without disrupting the normal process sequence. In the early stages granulation tissue is formed, later it is replaced by fibrous-reticular and coarse-fibrous bone tissue, and at the end – by a more mature lamellar bone tissue. However, the timing and degree of maturation of bone tissue, as well as osseointegration of implants in groups using low-frequency low-intensity pulsed ultrasound and without it differed significantly. The study showed that ultrasound exposure to implants and surrounding tissues induces osteoreparation processes, stimulating neoangiogenesis in granulation and newly formed bone tissue. Conclusion. Application of ultrasound to implants and subsequent application to the peri-implant tissues during and after dental implantation promotes the formation of bone tissue, identical by the histostructure to the maternal bone.

A influência da irradiação ultrassônica de baixa intensidade em cultura de células fibroblásticas

2015 ◽  
Vol 13 ◽  
pp. 306
Author(s):  
Larissa Dragonetti Bertin ◽  
Deise Aparecida Almeida Pires-Oliveira ◽  
Priscila Daniela Oliveira ◽  
Juliana Almeida Serpeloni ◽  
Stheace Kelly Fernandes Szezerbaty ◽  
...  
Keyword(s):  
Duty Cycle ◽  
Healing Process ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Fibroblast Cells ◽  
Pulse Regime ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Introduction: In vitro and in vivo put in evidence that the Low Intensity Pulsed Ultrasound therapy exerts a significant influence on cell function (cytoskeleton organization, stimulation of mitochondrial activity, ATP levels and plasma membrane). Objective: This study will analyze the radiation of low intensity pulsed ultrasound in fibroblast cells L 929. Method: In this study are presented the data from each exposure group average and standard deviation in each moment of evaluation (24 hours, 48 hours and 72 hours). The control group (received no radiation), 0.2 W/cm2 with 10% pulse regime (1: 9 duty cycle), 0.2 W / cm2 with 20% pulse regime (2: 8 cycle work), 0.4 W/cm2 with pulse scheme 10% (1: 9 duty cycle), 0.4 W/cm2 with pulse scheme 20% (2: 8 duty cycle). The analyzes will be performed through optical microscopy, MTT method 3 - (4,5-dimethylthiazol-2-yl) -2,5 diphenyl tetrazolium bromide, within the incubation times of 24, 48 and 72 hours. Results: Given the above study, the results presented in this project will be directed to increase the stimulation process and proliferation of fibroblast cells from the pulsed ultrasonic irradiation of low intensity, correlating with the healing process, neovascularization and repair. Conclusion: Therefore, the study of the effect of ultrasound from cell culture provides us with a simple and informative model on the significant aspects of the use of physical therapy in vivo system.

scholarly journals Effect of Low Intensity Pulsed Ultrasound (LIPUS) on Tooth Movement and Root Resorption: A Prospective Multi-Center Randomized Controlled Trial

2020 ◽  
Vol 9 (3) ◽  
pp. 804 ◽  
Author(s):  
Tarek El-Bialy ◽  
Khaled Farouk ◽  
Terry D. Carlyle ◽  
William Wiltshire ◽  
Robert Drummond ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Controlled Trial ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Control Side ◽  
Before And After ◽  
Group 1

The aim of this study was to evaluate the possible effect of low intensity pulsed ultrasound (LIPUS) on tooth movement and root resorption in orthodontic patients. Twenty-one patients were included in a split-mouth study design (group 1). Ten additional patients were included with no LIPUS device being used and this group was used as the negative control group (group 2). Group 1 patients were given LIPUS devices that were randomly assigned to right or left side on upper or lower arches. LIPUS was applied to the assigned side that was obtained by randomization, using transducers that produce ultrasound with a pulse frequency of 1.5 MHz, a pulse repetition rate of 1 kHz, and average output intensity of 30 mW/cm2. Cone-beam computed tomography (CBCT) images were taken before and after treatment. The extraction space dimensions were measured every four weeks and root lengths of canines were measured before and after treatment. The data were analyzed using paired t-test. The study outcome showed that the mean rate of tooth movement in LIPUS side was 0.266 ± 0.092 mm/week and on the control side was 0.232 ± 0.085 mm/week and the difference was statistically significant. LIPUS increased the rate of tooth movement by an average of 29%. For orthodontic root resorption, the LIPUS side (0.0092 ± 0.022 mm/week) showed a statistically significant decrease as compared to control side (0.0223 ± 0.022 mm/week). The LIPUS application accelerated tooth movement and minimized orthodontically induced tooth root resorption at the same time.

scholarly journals Effects of Low-Intensity Pulsed Ultrasound on the Proliferation of Human Umbilical Cord Mesenchymal Stem Cells and Their Chondrogenic Differentiation via Bmp2 /Smad Signaling Pathway

2020 ◽  
Author(s):  
Xuhong JIN ◽  
Hejie Wang ◽  
Jiaju Che ◽  
Xiafu Chen ◽  
Dengao Huang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Chondrogenic Differentiation ◽  
Blue Staining ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Proliferation Activity ◽  
Smad Signaling Pathway

Abstract BackgroundAlthough Low-intensity pulsed ultrasound (LIPUS) regiment has been applied to the treatment of cartilage injury clinically, the effect of LIPUS stress loading on the proliferation and differentiation of hUCMSCs is still controversial. Yet the specific mechanical mechanism of LIPUS stimulates cartilage differentiation of hUCMSCs has not been clarified.ObjectiveTo investigate the effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) via BMP2 /smad signaling pathway in vitro.MethodThe hUCMSCs at P4 were randomly divided into control group(no LIPUS), L30 group (30mW/cm2), L50 group (50mW/cm2), L80 group (80mW/cm2), each LIPUS group respectively loaded 5, 10, 20 min/d, the cell proliferation activity was detected by CCK-8 assay after continuous irradiation for 5 days. For chondrogenic differentiation, hUCMSCs were divided 4 groups as follows: control group, Noggin group, LIPUS group, Noggin+LIPUS group, LIPUS irradiation was given 50w/cm2, 5min/d. Cartilage formation after 3 weeks was evaluated by Alician blue staining for GAG and COL II immunohistochemical staining. Furthermore, RT-PCR analysis of Sox-9, COL II Aggrecan, BMP2, Smad1, Smad5 and Smad9. Result： (1) The proliferation activity of L50 group(50w/cm2 , 5min/d) was noticeably higher compared with other groups, Alcian blue staining of GAG , COL II fluorescent staining in the LIPUS group were much stronger, quantitative RT-PCR assays showed that the expression of COL II, GAG, Sox-9, smad1, smad5 and smad9 in LIPUS group was significantly increased. Conclusion：The suitable LIPUS irradiation can promote cell proliferation, 50mW/cm2 intensity at 5min/d is most significant. And it can promote chondrogenesis of hUCMSCs via upregulating MP2 /smads signaling pathway.

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