scholarly journals Cold-Triggered Induction of ROS- and Raffinose Metabolism in Freezing-Sensitive Taproot Tissue of Sugar Beet

2021 ◽  
Vol 12 ◽  
Author(s):  
Isabel Keller ◽  
Christina Müdsam ◽  
C. Martins Rodrigues ◽  
Dominik Kischka ◽  
Wolfgang Zierer ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Freezing Tolerance ◽  
Low Temperatures ◽  
Survival Rates ◽  
Protective Role ◽  
Winter Survival ◽  
Temperate Climate ◽  
Freezing Damage ◽  
Anatomy And Physiology ◽  
Freezing Temperatures

Sugar beet (Beta vulgaris subsp. vulgaris) is the exclusive source of sugar in the form of sucrose in temperate climate zones. Sugar beet is grown there as an annual crop from spring to autumn because of the damaging effect of freezing temperatures to taproot tissue. A collection of hybrid and non-hybrid sugar beet cultivars was tested for winter survival rates and freezing tolerance. Three genotypes with either low or high winter survival rates were selected for detailed study of their response to frost. These genotypes differed in the severity of frost injury in a defined inner region in the upper part of the taproot, the so-called pith. We aimed to elucidate genotype- and tissue-dependent molecular processes during freezing and combined analyses of sugar beet anatomy and physiology with transcriptomic and metabolite profiles of leaf and taproot tissues at low temperatures. Freezing temperatures induced strong downregulation of photosynthesis in leaves, generation of reactive oxygen species (ROS), and ROS-related gene expression in taproots. Simultaneously, expression of genes involved in raffinose metabolism, as well as concentrations of raffinose and its intermediates, increased markedly in both leaf and taproot tissue at low temperatures. The accumulation of raffinose in the pith tissue correlated with freezing tolerance of the three genotypes. We discuss a protective role for raffinose and its precursors against freezing damage of sugar beet taproot tissue.

scholarly journals Cold-triggered induction of ROS- and raffinose-related metabolism in freezing-sensitive taproot tissue of sugar beet

2021 ◽  
Author(s):  
Isabel Keller ◽  
Christina Müdsam ◽  
Cristina Martins Rodrigues ◽  
Dominik Kischka ◽  
Wolfgang Zierer ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Carbohydrate Metabolism ◽  
Survival Rates ◽  
Protective Role ◽  
Temperate Climate ◽  
Freezing Damage ◽  
Interior Region ◽  
Climate Zones ◽  
Frost Injury ◽  
Freezing Temperatures

AbstractSugar beet (Beta vulgaris subsp. vulgaris) is the exclusive source of sugar in the form of sucrose in temperate climate zones. There, sugar beet is grown as an annual crop from spring to autumn because of the damaging effect of freezing temperatures to taproot tissue. Natural and breeded varieties display variance in the degree of tolerance to freezing temperatures and genotypes with elevated tolerance to freezing have been isolated. Here we compare initial responses to frost between genotypes with either low and high winter survival rates. The selected genotypes differed in the severity of frost injury. We combined transcriptomic and metabolite analyses of leaf- and taproot tissues from such genotypes to elucidate mechanisms of the early freezing response and to dissect genotype- and tissue-dependent responses. Freezing temperatures induced drastic downregulation of photosynthesis-related genes in leaves but upregulation of genes related to minor carbohydrate metabolism, particularly of genes involved in raffinose metabolism in both, leaf and taproot tissue. In agreement with this, it has been revealed that raffinose and the corresponding intermediates, inositol and galactinol, increased markedly in these tissues. We found that genotypes with improved tolerance to freezing, showed higher accumulation of raffinose in a defined interior region within the upper part of the taproot, the pith, representing the tissue most susceptible to freeze damages. This accumulation was accompanied by specific upregulation of raffinose synthesizing enzymes in taproots, suggesting a protective role for raffinose and its precursors for freezing damage in sugar beet.

scholarly journals Differential Thermal Analysis of St. Augustinegrass

HortScience ◽  
1995 ◽  
Vol 30 (7) ◽  
pp. 1388-1389 ◽  
Author(s):  
H.W. Philley ◽  
C.E. Watson ◽  
J.V. Krans ◽  
J.M. Goatley ◽  
F.B. Matta
Keyword(s):  
Thermal Analysis ◽  
Differential Thermal Analysis ◽  
Freezing Tolerance ◽  
Field Trial ◽  
Narrow Range ◽  
Winter Survival ◽  
Stenotaphrum Secundatum ◽  
Lethal Temperatures ◽  
Freezing Temperatures

The objective of this study was to relate the lethal freezing temperatures of St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] genotypes, as measured by differential thermal analysis (DTA), to winter survival observed in the field. DTA-predicted lethal temperatures of 14 St. Augustinegrass genotypes ranged from –7.7 to –4.7C. Regression of field winter survival vs. DTA-predicted lethal temperatures resulted in an r2 = 0.57 for one field trial that evaluated cultivars with a relatively narrow range of expected freezing tolerance. In a second study evaluating cultivars with a greater range of freezing tolerance, r2 was 0.92 when winter survival was regressed on DTA-predicted lethal temperatures. DTA was successful in measuring freezing avoidance of St. Augustinegrass cultivars.

scholarly journals Metabolomic analyses reveal substances that contribute to the increased freezing tolerance of alfalfa (Medicago sativa L.) after continuous water deficit

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Hongyu Xu ◽  
Zhenyi Li ◽  
Zongyong Tong ◽  
Feng He ◽  
Xianglin Li
Keyword(s):  
Water Management ◽  
Amino Acids ◽  
Fatty Acids ◽  
Water Deficit ◽  
Freezing Tolerance ◽  
Low Temperatures ◽  
Unsaturated Fatty Acids ◽  
Soluble Sugar ◽  
Winter Survival ◽  
Economic Losses

Abstract Background Alfalfa is a high-quality forage cultivated widely in northern China. Recently, the failure of alfalfa plants to survive the winter has caused substantial economic losses. Water management has attracted considerable attention as a method for the potential improvement of winter survival. The aim of this study was to determine whether and how changes in the water regime affect the freezing tolerance of alfalfa. Results The alfalfa variety WL353LH was cultivated under water regimes of 80 and 25% of water-holding capacity, and all the plants were subjected to low temperatures at 4/0 °C (light/dark) and then − 2/− 6 °C (light/dark). The semi-lethal temperatures were lower for water-stressed than well-watered alfalfa. The pool sizes of total soluble sugars, total amino acids, and proline changed substantially under water-deficit and low-temperature conditions. Metabolomics analyses revealed 72 subclasses of differential metabolites, among which lipid and lipid-like molecules (e.g., fatty acids, unsaturated fatty acids, and glycerophospholipids) and amino acids, peptides, and analogues (e.g., proline betaine) were upregulated under water-deficit conditions. Some carbohydrates (e.g., D-maltose and raffinose) and flavonoids were also upregulated at low temperatures. Finally, Kyoto Encyclopedia of Genes and Genomes analyses revealed 18 significantly enriched pathways involved in the biosynthesis and metabolism of carbohydrates, unsaturated fatty acids, amino acids, and glycerophospholipids. Conclusions Water deficit significantly enhanced the alfalfa’ freezing tolerance, and this was correlated with increased soluble sugar, amino acid, and lipid and lipid-like molecule contents. These substances are involved in osmotic regulation, cryoprotection, and the synthesis, fluidity, and stability of the cellular membrane. Our study provides a reference for improving alfalfa’ winter survival through water management.

Influence of Planting Stock Clove Size, Nitrogen Rate, and Planting Method on Elephant Garlic Production

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 517f-518
Author(s):  
Jeanine M. Davis
Keyword(s):  
Survival Rates ◽  
Ground Level ◽  
Winter Survival ◽  
Nitrogen Rate ◽  
Planting Stock ◽  
Mild Winter ◽  
Allium Ampeloprasum ◽  
Planting Methods ◽  
Planting Method ◽  
Nitrogen Rates

To produce large elephant garlic (Allium ampeloprasum L.) bulbs in the southeastern United States, stock cloves must be planted in the fall. During extremely cold winters, however, winter survival rates can be very low. A 2-year study was undertaken to examine practices to increase winter survival rates. Two clove sizes (≤20 g or >20 g), three nitrogen rates (112, 224, and 336 kg/ha), and three planting methods (flat, mulched, and hilled) were tested in a RCB design with four replications. For all planting methods, cloves were set in a shallow trench and covered with soil to ground level. This was also the flat treatment. For the mulched treatment, 7 cm of straw was spread on top. For the hilled treatment, soil was mounded 10 to 15 cm high over the ground level. Cloves were planted in early October and harvested in mid-June. Use of large planting stock cloves increased winter survival rates during the harsh winter, but had no effect during the mild winter. Both years, winter survival was reduced with the flat treatment. Yields of marketable bulbs were 4 to 5 times higher when >20 g cloves were planted than when ≤20 g cloves were used. Nitrogen rate and planting method had no effect on yields. The >20 g cloves also produced larger bulbs than the smaller cloves. Of the three planting methods, the flat treatment produced the smallest bulbs. Bulbs were much larger following the mild winter than the harsh winter.

Factors Affecting Winter Survival of Johnsongrass (Sorghum halepense) Rhizomes

1991 ◽  
Vol 5 (1) ◽  
pp. 108-110 ◽  
Author(s):  
Robert G. Hartzler ◽  
Art Gover ◽  
Joanne Stellingwerf
Keyword(s):  
Survival Rates ◽  
Winter Survival ◽  
Sorghum Halepense ◽  
Factors Affecting ◽  
Rhizome Biomass ◽  
Depth Of Burial

There were no differences in johnsongrass rhizome winter survival at central and southeastern locations in Pennsylvania in 1988 or 1989. At a 6-cm depth of burial, survival rates of 5 and 25% were found for rhizome segments 7.5 and 15.0 cm in length, respectively. Survival rates for rhizomes buried 25 cm averaged 86%, with no difference in survival between rhizome lengths. The majority of rhizome biomass produced by the biotype used in these studies was found at soil depths allowing a high percentage of survival.

scholarly journals Effects of cryoprotectants and low temperatures on hatching and abnormal embryo development of Prochilodus lineatus (Characiformes: Prochilodontidae)

2017 ◽  
Vol 15 (3) ◽  
Author(s):  
Raphael S. Costa ◽  
Fabrício M. S. de Souza ◽  
José A. Senhorini ◽  
Rosicleire Veríssimo-Silveira ◽  
Alexandre Ninhaus-Silveira
Keyword(s):  
Embryonic Development ◽  
Low Temperatures ◽  
Survival Rates ◽  
Ice Nucleation ◽  
Small Bodies ◽  
Prochilodus Lineatus ◽  
Morphological Alterations ◽  
Membrane Rupture ◽  
Significant Difference ◽  
Chilling Sensitivity

ABSTRACT This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfoxide (Me2SO4) and glycerol (GLY), terminating in a solution of high osmolarity. There was no significant difference in the embryos survival of toxicity test between series of PROP and Me2SO4 in the 6S and 20S. In the cooling protocols, were evaluated the effects of low temperature associated with cryoprotectants. At 5ºC, PROP showed survival rates above 75% in the gastrula stage (G) and above 90% in the 6S and 20S stages. High rates of abnormalities were observed, and the most recurrent were: small bodies, fins presenting uncontrolled cell growth, membrane rupture, and retraction. These results demonstrate the need to use cryoprotectant solutions, even when there is no ice nucleation, and, on the other hand, shows that high cryoprotectant concentrations promote numerous morphological lesions, compromising normal embryonic development.

scholarly journals Ambient Temperature and Associations with Daily Visits to a Psychiatric Emergency Unit in Sweden

2019 ◽  
Vol 16 (2) ◽  
pp. 286 ◽  
Author(s):  
Hanne Carlsen ◽  
Anna Oudin ◽  
Steinn Steingrimsson ◽  
Daniel Oudin Åström
Keyword(s):  
Ambient Temperature ◽  
Psychiatric Disorders ◽  
Low Temperatures ◽  
Linear Models ◽  
Warm Season ◽  
Cold Season ◽  
Psychiatric Emergency ◽  
University Hospital ◽  
Temperate Climate ◽  
Urban Region

High or low ambient temperatures pose a risk factor for the worsening or onset of psychiatric disorders. The aim of this study was to investigate the association between ambient temperature and psychiatric emergency visits in an urban region in a temperate climate. The daily number of visits to a psychiatric emergency room (PEVs) at Sahlgrenska University Hospital, Gothenburg, Sweden and the daily mean temperature were extracted for the study period 1 July 2012 to 31 December 2017. Case-crossover analysis with distributed lag non-linear models was used to analyse the data by season. The warm season was defined as May to August and the cold season as November to February. Shorter lags periods were used for the warm season than the cold season. In the analysis, temperatures at the 95th percentile was associated with 14% (95% confidence interval (CI): 2%, 28%) increase in PEVs at lag 0–3 and 22% (95%CI: 6%, 40%) for lags 0–14 during the warm season, relative to the seasonal minimum effect temperature (MET). During the cold season temperatures at the 5th percentile were associated with 25% (95% CI: −8%, 13%) and 18% (95% CI: −30%, 98%) increase in PEVs at lags 0–14 and 0–21 respectively. We observed an increased number of PEVs at high and low temperatures; however, not to a statistically significant extent for low temperatures. Our findings are similar to what has been found for somatic diseases and in studies of other mental health outcomes in regions with more extreme climates. This merits the inclusion of individuals with psychiatric disorders in awareness planning for climate warning systems.

scholarly journals Generation and Characterization of Universal Live-Attenuated Influenza Vaccine Candidates Containing Multiple M2e Epitopes

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 648
Author(s):  
Tatiana Kotomina ◽  
Irina Isakova-Sivak ◽  
Ki-Hye Kim ◽  
Bo Ryoung Park ◽  
Yu-Jin Jung ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Influenza A ◽  
Tandem Repeats ◽  
Survival Rates ◽  
Protective Role ◽  
Influenza Viruses ◽  
Influenza Vaccines ◽  
Cell Mediated Immunity ◽  
Universal Vaccine ◽  
Specific Antibodies

Influenza viruses constantly evolve, reducing the overall protective effect of routine vaccination campaigns. Many different strategies are being explored to design universal influenza vaccines capable of protecting against evolutionary diverged viruses. The ectodomain of influenza A M2e protein (M2e) is among the most promising targets for universal vaccine design. Here, we generated two recombinant live attenuated influenza vaccines (LAIVs) expressing additional four M2e tandem repeats (4M2e) from the N-terminus of the viral hemagglutinin (HA) protein, in an attempt to enhance the M2e-mediated cross-protection. The recombinant H1N1+4M2e and H3N2+4M2e viruses retained growth characteristics attributable to traditional LAIV viruses and induced robust influenza-specific antibody responses in BALB/c mice, although M2e-specific antibodies were raised only after two-dose vaccination with LAIV+4M2e viruses. Mice immunized with either LAIV or LAIV+4M2e viruses were fully protected against a panel of heterologous influenza challenge viruses suggesting that antibody and cell-mediated immunity contributed to the protection. The protective role of the M2e-specific antibody was seen in passive serum transfer experiments, where enhancement in the survival rates between classical LAIV and chimeric H3N2+4M2e LAIV was demonstrated for H3N2 and H5N1 heterologous challenge viruses. Overall, the results of our study suggest that M2e-specific antibodies induced by recombinant LAIV+4M2e in addition to cellular immunity by LAIV play an important role in conferring protection against heterologous viruses.

Cold-Hardiness, Habitat and Winter Survival of Some Orchard Arthropods in Nova Scotia

1964 ◽  
Vol 96 (4) ◽  
pp. 617-625 ◽  
Author(s):  
A. W. MacPhee
Keyword(s):  
Nova Scotia ◽  
Air Temperature ◽  
Low Temperatures ◽  
Cold Hardiness ◽  
Winter Survival ◽  
Kings County ◽  
Winter Conditions ◽  
Cold Hardy

AbstractIn Kings County, Nova Scotia, low temperatures in the coldest nights of winter can differ by as much as 10°F. from one area to another. This has an important bearing on winter survival of some arthropods. Overwintering sites of orchard arthropods range from exposed situations which remain at air temperature to well protected ones on the ground where temperatures rarely go below 20°F. The cold-hardiness of each of 24 species of arthropods was measured: seven were sufficiently cold-hardy to survive any winter conditions in Nova Scotia, five were less cold-hardy but overwinter in well protected sites and twelve had marginal cold-hardiness, their mortality varying with the winter and the locality.

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