scholarly journals Genomic Informed Breeding Strategies for Strawberry Yield and Fruit Quality Traits

2021 ◽  
Vol 12 ◽  
Author(s):  
Helen M. Cockerton ◽  
Amanda Karlström ◽  
Abigail W. Johnson ◽  
Bo Li ◽  
Eleftheria Stavridou ◽  
...  

Over the last two centuries, breeders have drastically modified the fruit quality of strawberries through artificial selection. However, there remains significant variation in quality across germplasm with scope for further improvements to be made. We reported extensive phenotyping of fruit quality and yield traits in a multi-parental strawberry population to allow genomic prediction and quantitative trait nucleotide (QTN) identification, thereby enabling the description of genetic architecture to inform the efficacy of implementing advanced breeding strategies. A negative relationship (r = −0.21) between total soluble sugar content and class one yield was identified, indicating a trade-off between these two essential traits. This result highlighted an established dilemma for strawberry breeders and a need to uncouple the relationship, particularly under June-bearing, protected production systems comparable to this study. A large effect of quantitative trait nucleotide was associated with perceived acidity and pH whereas multiple loci were associated with firmness. Therefore, we recommended the implementation of both marker assisted selection (MAS) and genomic prediction to capture the observed variation respectively. Furthermore, we identified a large effect locus associated with a 10% increase in the number of class one fruit and a further 10 QTN which, when combined, are associated with a 27% increase in the number of marketable strawberries. Ultimately, our results suggested that the best method to improve strawberry yield is through selecting parental lines based upon the number of marketable fruits produced per plant. Not only were strawberry number metrics less influenced by environmental fluctuations, but they had a larger additive genetic component when compared with mass traits. As such, selecting using “number” traits should lead to faster genetic gain.

2013 ◽  
Vol 38 (3) ◽  
pp. 425-433
Author(s):  
Shamima Nasreen ◽  
R Ahmed ◽  
MA Ullah ◽  
MA Hoque

The study was carried out at the farmer's field of Juri Upazilla, Maulavibazar District during 2008-2009, 2009-2010, and 2011-2012 to find out the proper combination of fertilizer nutrients that promotes better fruit yield and quality of mandarin (cv. Khashi Kamla). The treatments were Native nutrient i.e., control (TI), N150P50K150Mg30 (T2), N200P75K175Mg40 (T3), N250P100 K200Mg50 (T4), N300P125K225Mg60 (T5), and N200P75K175Mg0 (T6) g/plant/year. In addition, 20 kg cowdung/plant was used as blanket dose. Yield components, yield, and TSS content (%) of mandarin varied significantly due to variation of nutrients in all the test years. Fruit yield/plant was improved significantly with increase in NPK and Mg dose irrespective of years. Application of Mg in combination with NPK increased 17-157% mean fruit yield over magnesium control. Significantly the highest yield and yield attributes were recorded under treatment N300 P125 K225 Mg60 g/plant (T5) along with 20 kg cowdung/plant in all the years. Total soluble sugar (%) content was also maximum in trees receiving N300P125K225Mg30 g/plant. The highest gross margin and marginal rate of return were achieved by the same treatment (T5). The lowest fruit yield/plant was obtained from untreated control plot (native nutrient). Three years’ study revealed that application of N300P125K225Mg60 along with 20 kg cowdung/plant would be economically optimum for achieving higher yield and better fruit quality in mandarin grown under piedmont plain soil. DOI: http://dx.doi.org/10.3329/bjar.v38i3.16969 Bangladesh J. Agril. Res. 38(3): 425-433, September 2013


2021 ◽  
Author(s):  
Helen M. Cockerton ◽  
Amanda Karlström ◽  
Abigail W. Johnson ◽  
Bo Li ◽  
Eleftheria Stavridou ◽  
...  

AbstractOver the last two centuries breeders have drastically modified the fruit quality of strawberries through artificial selection. However, there remains significant variation in quality across germplasm with scope for further improvements to be made. We report extensive phenotyping of fruit quality and yield traits in a multi-parental strawberry population to allow genomic prediction and QTL identification, thereby enabling the description of genetic architecture to inform the efficacy of implementing advanced breeding strategies.A trade-off was observed between two essential traits: sugar content and class one yield. This result highlights an established dilemma for strawberry breeders and a need to uncouple the relationship, particularly under June-bearing, protected production systems comparable to this study. A large effect QTL was associated with perceived acidity and pH whereas multiple loci were associated with firmness, we therefore recommend the implementation of both MAS and genomic prediction to capture the observed variation respectively.Ultimately, our results suggest that the best method to improve strawberry yield is through selecting parental lines based upon the number of marketable fruit produced per plant. Strawberry number metrics were less influenced by environmental fluctuations and had a larger additive genetic component when compared to mass traits. As such, selecting using “number” traits should lead to faster genetic gain. Finally, we identify a large effect locus associated with an increase in class one fruit.


Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4884
Author(s):  
Khadijeh Yasaminshirazi ◽  
Jens Hartung ◽  
Michael Fleck ◽  
Simone Graeff-Hoenninger

The growing interest of consumers in healthy organic products has increased the attention to the organic production of beetroot. In this regard, six field experiments were conducted in 2017 and 2018 in three different locations under the specific conditions of organic agriculture, and fifteen beetroot genotypes, including one F1 hybrid as a commercial control and one breeding line, were compared regarding the content of the total dry matter, total soluble sugar, nitrate, betalain, and total phenolic compounds in order to investigate the genetic potential of new and existing open-pollinated genotypes of beetroot regarding the content of their bioactive compounds. The results of this study indicated a significant impact of genotype (p < 0.05) on all measured compounds. Furthermore, results revealed a significant influence of the interactions of location × year (p < 0.05) on the beetroot composition, and, thus, the role of environmental conditions for the formation of tested compounds. The total dry matter content (TDMC) of beetroots varied between 14.12% and 17.50%. The genotype ‘Nochowski’, which possessed the highest total soluble sugar content with 14.67 °Bx (Brix), was among the genotypes with the lowest nitrate content. On the contrary, the cylindrical-shaped genotype ‘Carillon RZ’ (Rijk Zwaan), indicated the lowest sugar content and the highest nitrate concentration. The amount of total phenolic compounds ranged between 352.46 ± 28.24 mg GAE 100 g−1 DW (milligrams of gallic acid equivalents per 100 g of dry weight) and 489.06 ± 28.24 mg GAE 100 g−1 DW for the red-colored genotypes which is correlated with the high antioxidant capacity of the investigated genotypes. Due to the specifics of the required content of bioactive compounds for various products, the selection of suitable genotypes should be aligned with the intended final utilization.


1970 ◽  
Vol 16 ◽  
pp. 95-99 ◽  
Author(s):  
Md Rezaul Karim ◽  
MMH Khan ◽  
Md Salim Uddin ◽  
NK Sana ◽  
F Nikkon ◽  
...  

Significant differences were found in sugar content and carbohydrate splitting enzyme activities in tubers of ten indigenous potato varieties at harvesting and after keeping at cold storage. The activities of invertase, amylase, β-galactosidase and cellulase in all varieties were found to be increased by 2-12, 1.2-4, 1.9-4.5, and 1.1-3.7 folds, respectively from harvesting to cold stored potatoes. The amount of starch and sucrose were found to be decreased by 1.15-2.8 and 1.02-1.4 folds, respectively from harvesting to cold stored in all varieties. Total soluble sugar and reducing sugar contents in potatoes were increased by 1.02-1.4 and 4-11 folds, respectively from harvesting to cold stored in all varieties of potatoes. The amount of reducing sugar increased in cold stored potatoes due to the increased activities of carbohydrate splitting enzymes. Key words: Potatoes, Indigenous, Carbohydrate splitting enzymes, Sugars. DOI:10.3329/jbs.v16i0.3748 J. bio-sci. 16: 95-99, 2008


2016 ◽  
Vol 2 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Md Moniruzzaman Sohag Howlader ◽  
Sheikh Rashel Ahmed ◽  
Khadizatul Kubra ◽  
Md Khairul Hassan Bhuiyan

The present study was aimed to evaluate biochemical and phytotochemical of dry leaves of Stevia rebaudiana. Biochemical analysis indicated that Stevia leaves are a good source of carbohydrate and reducing sugar. Different extraction methods were used to prepare four different dry extracts (Extract A, B, C, D). Total soluble sugars and reducing sugars were analyzed for these four dry extracts and commercial Stevia powder. The highest amount of total soluble sugar (477 mg sugar g-1 dry extract) was obtained from extract C and higher amount of reducing sugar (82 mg g-1 dry extract) was obtained from extract D among the extracts. But commercial Stevia powder showed higher total soluble sugar content (754 mg g-1 dry powder) and highest amount of reducing sugar (98 mg g-1 dry extract) than all the extracted dry samples. The extraction process of dry extract C was feasible for the extraction of total soluble sugar. For the phytochemical screening, crude extract was tested for the presence of different chemical groups and presence of alkaloids, phenolic compounds, steroids, tannins, flavonoids, cardiac glycosides and saponins that were identified. The highest amount of total phenolic compounds (92 mg) was recorded from methanolic extract of extraction B. The lowest amount of total phenolic compounds (36 mg) was recorded in ethanolic extract of extraction A. So, Methanol proved as best solvent to extract increased quantity of total phenolic compounds than other solvents.Asian J. Med. Biol. Res. March 2016, 2(1): 121-130


2006 ◽  
Vol 33 (3) ◽  
pp. 279 ◽  
Author(s):  
María E. Balibrea ◽  
Cristina Martínez-Andújar ◽  
Jesús Cuartero ◽  
María C. Bolarín ◽  
Francisco Pérez-Alfocea

Soluble sugar content has been studied in relation to sucrose metabolism in the hexose-accumulating cultivated tomato Lycopersicon esculentum Mill, the wild relative species Lycopersicon cheesmanii Riley, in the sucrose-accumulating wild relative species Lycopersicon chmielewskii Rick, Kesicky, Fobes & Holle. and in two hexose-accumulating interspecific F1 hybrids (L. esculentum × L. cheesmanii; L. esculentum × L. chmielewskii), cultivated under two irrigation regimes (control: EC = 2.1 and saline: EC = 8.4 dS m–1). Under control conditions the total soluble sugar content (as hexose equivalents) in the ripe fruits of L. cheesmanii was 3-fold higher than in L. esculentum, while L. chmielewskii and both F1 hybrids contained twice as much as the cultivar. With the exception of L. esculentum × L. cheesmanii, salinity increased the sugar content by 1.3 (wild species) and 1.7 times (cultivar and L. esculentum × L. chmielewskii) with respect to control fruits. Wild germplasm or salinity provided two different mechanisms for the increases in fruit sugar content. The hexoses accumulated in ripe fruits were strongly influenced by those accumulated at the start of ripening, but the hydrolysed starch before start of ripening only partially explained the final hexose levels and especially the increase under salinity. The early cell wall acid invertase and the late neutral invertase activities appeared to be related to the amount of hexoses accumulated in ripe fruits. However, no metabolic parameter was positively related to the amount of sugar accumulated (including sucrose). The major differences between genotypes appeared in ripe fruits, in which up to 50% of the total amount of sugars accumulated in the wild species (mainly in L. cheesmanii) and hybrids cannot be explained by the sugars accumulated and the starch hydrolysed before the start of ripening stage. As a consequence, the higher fruit quality of the wild species compared with L. esculentum may depend more on the continuation of sucrose import during ripening than on osmotic or metabolic particularities such as the hexose / sucrose-accumulator character or specific enzyme activities.


2021 ◽  
Vol 49 (3) ◽  
pp. 12445
Author(s):  
Daonapa CHUNGLOO ◽  
Rujira TISARUM ◽  
Thapanee SAMPHUMPHUANG ◽  
Thanyaporn SOTESARITKUL ◽  
Suriyan CHA-UM

Paclobutrazol (PBZ) is a member of plant growth retardants, commonly applied for growth regulation, yield improvement, and biotic and abiotic stress alleviation. However, the effects of PBZ on turmeric (Curcuma longa L.; Zingiberaceae), a rhizomatous herb, have not been well established. The objective of this investigation was to gain a better understanding of the effect of PBZ on two different varieties of turmeric plants, ‘Surat Thani’ (‘URT’; high curcuminoids >5% w/w) and ‘Pichit’ (‘PJT’; low curcuminoids <3% w/w). Pseudostem height of cv. ‘PJT’ treated by 340 µM PBZ was significantly decreased by 14.82% over control, whereas it was unchanged in cv. ‘URT’. Interestingly, leaf greenness (SPAD value), maximum quantum yield of PSII (Fv/Fm) and photon yield of PSII (ΦPSII) in cv. ‘PJT’ treated by 340 µM PBZ were significantly elevated by 1.47, 1.28 and 1.23 folds, over control respectively. Net photosynthetic rate (Pn) in cv. ‘PJT’ declined by 38.58% (340 µM PBZ) over control, as a result of low levels of total soluble sugars (TSS; 127.8 mg g-1 DW) in turmeric rhizome. A positive relation between photosynthetic abilities and aerial fresh weight was demonstrated. In addition, a negative relationship between TSS and total curcuminoids was evidently found (R2 = 0.4524). Curcuminoids yield in turmeric rhizomes significantly dropped, depending on the degree of exogenous foliar PBZ applications. In summary, cv. PJT was found to be very sensitive to PBZ application, whereas rhizome yield and growth traits and high amount of curcuminoids were retained in cv. ‘URT’. Plant growth retention in turmeric cv. ‘URT’ using 170 mM PBZ foliar spray without negative effects on rhizome biomass and total curcuminoids content was demonstrated.


2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Ouyang ◽  
Xuewu Sun ◽  
Yanning Tan ◽  
Zhizhong Sun ◽  
Dong Yu ◽  
...  

Remobilization of pre-anthesis NSCs (non-structural carbohydrates) is significant for effective grain filling in rice (Oryza sativa L.). However, abundant starch particles as an important component of NSCs are still present in the leaf sheath and stem at the late stage of grain filling. There are no studies on how bioengineering techniques can be used to improve the efficiency of NSC remobilization. In this study, RAmy1A was expressed under the senescence-specific promoter of SAG12, which was designed to degrade starch in the leaf sheath and stem during grain filling. RAmy1A mRNA successfully accumulated in the leaf, stem, and sheath of transgenic plants after anthesis. At the same time, the starch and total soluble sugar content in the leaf, stem, and leaf sheath were obviously decreased during the grain-filling period. The photosynthetic rate of transgenic lines was higher than that of the wild types by an average of 4.0 and 9.9%, at 5 and 10 days after flowering, respectively. In addition, the grain-filling rate of transgenic lines was faster than that of the wild types by an average of 26.09%. These results indicate an enhanced transport efficiency of NSCs from source tissues in transgenic rice. Transgenic rice also displayed accelerated leaf senescence, which was hypothesized to contribute to decreased grain weight.


2017 ◽  
Vol 8 (1) ◽  
pp. 99 ◽  
Author(s):  
Cíntia Martins De Oliveira ◽  
Welson Lima Simões ◽  
Paulo Roberto Coelho Lopes ◽  
José Bezerra Da Silva ◽  
Emanoel Jurema Araújo ◽  
...  

The objective of this study was to evaluate the effect of different irrigation levels on flowering, fruit set, carbohydrate and protein content and gas exchange, of apple varietiesin the Brazilian semiarid region. The experimental design was a randomized block with split plots with five replications and four plot irrigation levels (60, 80, 100, and 120% of the reference evapotranspiration (ETo)) and the subplots with two cultivars (Juliet and Princess). As a result, it was found that the number of flowers (NFL), fruits (NFR), shoot percentage (SP) and fruit set (FS) were higher in cv. Juliet. The number of buds (NB) and the fertility index (FI) were higher in cv. Princess. With regard to metabolites, reducing sugar content (RS) had no significant effect among cultivars. The non-reducing sugar content (NRS), total soluble sugar (TSS) and total soluble proteins (TSP) were higher in cv. Princess. As for irrigation depths, TSP, sprouting, flowering and fruit set increased along with the amount of water applied, while the RS, NRS and TSS contents had the opposite behavior.


2000 ◽  
Vol 10 (1) ◽  
pp. 162-168 ◽  
Author(s):  
Richard Allen Hamman ◽  
Imed Eddine Dami

Field studies were conducted to determine the effect of three drip irrigation regimes on grapevine growth, juice and wine quality, soil moisture, cold hardiness of bud and cane tissues and soluble sugar content of cortical cane tissues of Vitis vinifera, Linnaeus `Cabernet Sauvignon'. This study was developed to help provide some irrigation management strategies that would improve fruit quality and reduce excessive vigor. Irrigation treatments of 192, 96, and 48 L (51, 25, and 13 gal) per vine per week were initiated at bud break until veraison (initiation of berry color) and then reduced by 25% through harvest. Significant differences of fruit weight per vine, crop load, soil moisture, average berry and cluster weight, shoot length and pruning weight per meter of canopy row were observed among treatments. Juice and wine compositions and wine color were also significantly different; however, cold hardiness and soluble sugar contents did not differ between treatments.


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