Transformation and Overexpression of Primary Cell Wall Synthesis-Related Zinc Finger Gene Gh_A07G1537 to Improve Fiber Length in Cotton

Molecular interventions have helped to explore the genes involved in fiber length, fiber strength, and other quality parameters with improved characteristics, particularly in cotton. The current study is an extension and functional validation of previous findings that Gh_A07G1537 influences fiber length in cotton using a chromosomal segment substitution line MBI7747 through RNA-seq data. The recombinant Gh_A07G1537 derived from the MBI7747 line was over-expressed in CCRI24, a genotype with a low profile of fiber quality parameters. Putative transformants were selected on MS medium containing hygromycin (25mg/ml), acclimatized, and shifted to a greenhouse for further growth and proliferation. Transgene integration was validated through PCR and Southern Blot analysis. Stable integration of the transgene (ΔGh_A07G1537) was validated by tracking its expression in different generations (T0, T1, and T2) of transformed cotton plants. It was found to be 2.97-, 2.86-, and 2.92-folds higher expression in T0, T1, and T2 plants, respectively, of transgenic compared with non-transgenic cotton plants. Fiber quality parameters were also observed to be improved in the engineered cotton line. Genetic modifications of Gh_A07G1537 support the improvement in fiber quality parameters and should be appreciated for the textile industry.

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Non–2,4-D–resistant cotton response to glyphosate plus 2,4-D choline tank contamination

Weed Technology ◽

10.1017/wet.2019.85 ◽

2019 ◽

Vol 34 (1) ◽

pp. 82-88

Author(s):

Misha R. Manuchehri ◽

Peter A. Dotray ◽

J. Wayne Keeling ◽

Gaylon D. Morgan ◽

Seth A. Byrd

Keyword(s):

Fiber Length ◽

Yield Loss ◽

Fiber Strength ◽

Fiber Quality ◽

Field Trials ◽

Quality Parameters ◽

Lint Yield ◽

Contamination Rate ◽

Financial Return ◽

Application Timing

AbstractField trials were conducted near Lubbock, TX, in 2013, 2014, and 2015 to evaluate non–2,4-D–resistant cotton response to low rates of glyphosate plus 2,4-D choline. Cotton was treated with five rates of glyphosate plus 2,4-D choline (0.0183, 0.183, 1.83, 18.3, and 183 g ae ha−1) at two application timings (nine leaf and first bloom). These rates correspond to contamination rates of 0.0008%, 0.008%, 0.08%, 0.8%, and 8%, respectively. Visual cotton injury, boll retention, lint yield, and fiber properties were recorded. When averaged over contamination rates, visual injury after applications made to nine-leaf cotton was greater than for first-bloom cotton in three of 3 yr and yield loss was greater when applications were made to nine-leaf cotton when compared with first-bloom cotton in two of 3 yr. Averaged over application timing, lint yield in 2013, 2014, and 2015 after glyphosate plus 2,4-D choline contamination rates of 0.0008% and 0.008% were not different than that of the nontreated control, whereas contamination rates of 0.08%, 0.8%, and 8% decreased yield by 3% to 20%, 45% to 58%, and 80% to 96%, respectively. Contamination rates of 0.0008%, 0.008%, 0.08%, and 0.8% rarely affected fiber quality; however, a contamination rate of 8% frequently decreased micronaire, fiber length, fiber length uniformity, and fiber strength. This decrease in fiber quality also resulted in a reduction in cotton loan value and potential financial return. Although decreases in fiber quality parameters were not observed with the 0.8% contamination rate, significant reductions in financial return occurred due to yield loss caused by injury from glyphosate plus 2,4-D choline.

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Genome-Wide Association Analysis Reveals Loci and Candidate Genes Involved in Fiber Quality Traits Under Multiple Field Environments in Cotton (Gossypium hirsutum)

Frontiers in Plant Science ◽

10.3389/fpls.2021.695503 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xiaohui Song ◽

Guozhong Zhu ◽

Sen Hou ◽

Yamei Ren ◽

Muhammad Waqas Amjid ◽

...

Keyword(s):

Candidate Genes ◽

Fiber Length ◽

Fiber Strength ◽

Fiber Quality ◽

Quality Parameters ◽

Fiber Development ◽

Genome Wide Association ◽

Quality Traits ◽

Fiber Quality Traits ◽

Genome Wide

Fiber length, fiber strength, and fiber micronaire are the main fiber quality parameters in cotton. Thus, mining the elite and stable loci/alleles related to fiber quality traits and elucidating the relationship between the two may accelerate genetic improvement of fiber quality in cotton. Here, genome-wide association analysis (GWAS) was performed for fiber quality parameters based on phenotypic data, and 56,010 high-quality single nucleotide polymorphisms (SNPs) using 242 upland cotton accessions under 12 field environments were obtained. Phenotypic analysis exhibited that fiber length (FL) had a positive correlation with fiber strength (FS) and had a negative correlation with fiber micronaire (Mic). Genetic analysis also indicated that FL, FS, and Mic had high heritability of more than 80%. A total of 67 stable quantitative trait loci (QTLs) were identified through GWAS analysis, including 31 for FL, 21 for FS, and 22 for Mic. Of them, three pairs homologous QTLs were detected between A and D subgenomes, and seven co-located QTLs with two fiber quality parameters were found. Compared with the reported QTLs, 34 co-located with previous studies, and 33 were newly revealed. Integrated with transcriptome analysis, we selected 256, 244, and 149 candidate genes for FL, FS, and Mic, respectively. Gene Ontology (GO) analysis showed that most of the genes located in QTLs interval of the three fiber quality traits were involved in sugar biosynthesis, sugar metabolism, microtubule, and cytoskeleton organization, which played crucial roles in fiber development. Through correlation analysis between haplotypes and phenotypes, three genes (GH_A05G1494, GH_D11G3097, and GH_A05G1082) predominately expressed in fiber development stages were indicated to be potentially responsible for FL, FS, and Mic, respectively. The GH_A05G1494 encoded a protein containing SGS-domain, which is related to tubulin-binding and ubiquitin-protein ligase binding. The GH_D11G3097 encoded 20S proteasome beta subunit G1, and was involved in the ubiquitin-dependent protein catabolic process. The GH_A05G1082 encoded RAN binding protein 1 with a molecular function of GTPase activator activity. These results provide new insights and candidate loci/genes for the improvement of fiber quality in cotton.

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Transplanting improves the allometry and fiber quality of Bt cotton in cotton–wheat cropping system

Experimental Agriculture ◽

10.1017/s0014479719000073 ◽

2019 ◽

Vol 56 (1) ◽

pp. 26-36

Author(s):

Muhammad Asghar Shah ◽

Mubshar Hussain ◽

Muhammad Shahzad ◽

Khawar Jabran ◽

Sami Ul-Allah ◽

...

Keyword(s):

Fiber Length ◽

Fiber Strength ◽

Management Practice ◽

Cropping Systems ◽

Fiber Quality ◽

Cropping System ◽

Bt Cotton ◽

Area Index ◽

Fiber Fineness

AbstractIn cotton–wheat cropping system of Pakistan, wheat (Triticum aestivum L.) is harvested in late April; however, the optimum sowing time of Bt cotton is mid-March. This indicates a time difference of 4–6 weeks between the harvest of wheat and cotton sowing. It is hypothesized that this overlapping period may be managed by transplanting cotton seedlings (30–45 days old) in late April, after the harvest of wheat due to better performance of already established seedlings. To this end, this study was conducted to evaluate the allometric traits and fiber quality of transplanted Bt cotton after harvesting wheat in the cotton–wheat cropping system. The Bt cotton–wheat cropping systems were flat sown wheat (FSW)–conventionally tilled cotton, FSW–zero tilled cotton, ridge sown wheat–ridge transplanted cotton using 30- and 45-days-old seedlings, and bed sown wheat (BSW)–bed transplanted cotton (BTC) also using 30- and 45-days-old seedlings. The study was conducted at Vehari and Multan in Punjab, Pakistan. Bt cotton in BSW–BTC with 45-days-old seedlings showed better performance for allometric (leaf area index; (LAI), net assimilation rate; (NAR), and crop growth rate; (CGR)), seed cotton yield, and fiber traits (fiber uniformity, fiber length, fiber strength, and fiber fineness) in comparison to other treatments. Most of the fiber quality traits were positively correlated with allometric traits and biological yield (dry matter yield at maturity) at both locations, except correlations of CGR and LAI with fiber fineness and fiber length and NAR with fiber length. As plant growth and fiber quality of transplanted cotton was significantly higher than conventionally grown cotton, our data indicate transplanting is an interesting management practice for improving productivity in wheat–cotton cropping systems.

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Phenotypic adaptability of cotton genotypes to the brazilian cerrado for yield and fiber quality

Bioscience Journal ◽

10.14393/bj-v36n4a2020-48148 ◽

2020 ◽

Vol 36 (4) ◽

Author(s):

Mayara Fávero Cotrim ◽

Francisco José Correa Farias ◽

Luiz Paulo de Carvalho ◽

Larissa Pereira Ribeiro Teodoro ◽

Carlos Antonio da Silva Junior ◽

...

Keyword(s):

Fiber Length ◽

Fiber Strength ◽

Fiber Quality ◽

Block Design ◽

Brazilian Cerrado ◽

Gxe Interaction ◽

Randomized Block Design ◽

Cotton Genotypes ◽

High Predictability ◽

Fiber Traits

Studies on the adaptability and stability are fundamental for plant breeding as they are an alternative to reduce the effects of genotypes x environments interaction (GxE). Moreover, they help identify cultivars with predictable behavior, which are responsive to environmental improvements, subsidizing cultivar recommendation. This study aimed to investigate the genotypes x environments interaction in cotton genotypes grown in the Brazilian Cerrado and identify genotypes for favorable and unfavorable environments. During the 2013/2014 and 2014/2015 seasons, 19 competition trials were carried out with cotton in a randomized block design, with 12 treatments, and four replications. The traits cotton seed yield, fiber percentage, fiber length, and fiber strength were evaluated. Results revealed significant GxE interaction for all the fiber traits evaluated. Genotype BRS 369 RF revealed general adaptability and high predictability for the fiber traits evaluated.

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DETERMINATION OF THE FACTORS LIMITING COTTON FIBER QUALITY IN TURKEY

Journal of Applied Life Sciences and Environment - Summer ◽

10.46909/journalalse-2021-009 ◽

2021 ◽

Vol 185 (1) ◽

pp. 85-99

Author(s):

Seyhan YASAR ◽

Emine KARADEMIR

Keyword(s):

Textile Industry ◽

Fiber Strength ◽

Fiber Quality ◽

Short Fiber ◽

Light Yellow ◽

Fiber Fineness ◽

Two Samples ◽

Mature Group ◽

Cotton Fiber Quality ◽

Fiber Maturity

This study aims to determine the variation of fiber quality in cotton varieties produced in the Southeastern Anatolia Region and Sanliurfa, Diyarbakir provinces. 1090 fiber samples were obtained from 6 cotton varieties (Lima, Stoneville 468, Candia and Babylon for Sanliurfa, Lima, Stoneville 468, Lodos and Gloria for Diyarbakir) collected from ginning factories in Sanliurfa and Diyarbakir. Statistical analyzes were done with HVI device and obtained data were analyzed by using Excel and TOTEMSTAT programs. In the frequency distribution, cotton varieties of the region are in the medium and long fiber group in terms of fiber length. They were in the medium (only two samples), strong and very strong group in terms of fiber strength. They were generally in the medium and thick group in terms of fiber fineness (micronaire). In terms of fiber uniformity index, the majority of the fibers were in the middle group. In terms of short fiber index, most of the fibers were in the very low and low groups. The majority of the samples were in the high and medium group in terms of fiber elongation, in the mature and very mature group in terms of fiber maturity. In terms of spinning consistency index (SCI) 59,2% of the fibers were between 119,41 and 135,83; 31,3% of them, were between 135,83 and 152,24, 58,2% of the material has a reflectance value of 74 and above. All materials were in white and light-yellow groups in terms of yellowness. It has been observed that the majority of the fibers (66%) are in the low group in terms of trash count. The results obtained from the study of cotton produce of Southeastern Anatolia Region of Turkey has shown that good fiber quality and to meet the demand of textile industry.

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Identification of Candidate Cotton Genes Associated With Fiber Length Through Quantitative Trait Loci Mapping and RNA-Sequencing Using a Chromosome Segment Substitution Line

Frontiers in Plant Science ◽

10.3389/fpls.2021.796722 ◽

2021 ◽

Vol 12 ◽

Author(s):

Quanwei Lu ◽

Xianghui Xiao ◽

Juwu Gong ◽

Pengtao Li ◽

Yan Zhao ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Candidate Gene ◽

Quantitative Trait ◽

Fiber Length ◽

Fiber Quality ◽

Substitution Line ◽

Chromosome Segment ◽

Chromosome Segment Substitution Line ◽

Trait Loci ◽

Segment Substitution

Fiber length is an important determinant of fiber quality, and it is a quantitative multi-genic trait. Identifying genes associated with fiber length is of great importance for efforts to improve fiber quality in the context of cotton breeding. Integrating transcriptomic information and details regarding candidate gene regions can aid in candidate gene identification. In the present study, the CCRI45 line and a chromosome segment substitution line (CSSL) with a significantly higher fiber length (MBI7747) were utilized to establish F2 and F2:3 populations. Using a high-density genetic map published previously, six quantitative trait loci (QTLs) associated with fiber length and two QTLs associated with fiber strength were identified on four chromosomes. Within these QTLs, qFL-A07-1, qFL-A12-2, qFL-A12-5, and qFL-D02-1 were identified in two or three environments and confirmed by a meta-analysis. By integrating transcriptomic data from the two parental lines and through qPCR analyses, four genes associated with these QTLs including Cellulose synthase-like protein D3 (CSLD3, GH_A12G2259 for qFL-A12-2), expansin-A1 (EXPA1, GH_A12G1972 for qFL-A12-5), plasmodesmata callose-binding protein 3 (PDCB3, GH_A12G2014 for qFL-A12-5), and Polygalacturonase (At1g48100, GH_D02G0616 for qFL-D02-1) were identified as promising candidate genes associated with fiber length. Overall, these results offer a robust foundation for further studies regarding the molecular basis for fiber length and for efforts to improve cotton fiber quality.

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Cotton (Gossypium Hirsutum) Response to DPX-PE350 Applied Postemergence

Weed Technology ◽

10.1017/s0890037x00037052 ◽

1993 ◽

Vol 7 (1) ◽

pp. 159-162 ◽

Cited By ~ 23

Author(s):

David L. Jordan ◽

Robert E. Frans ◽

Marilyn R. McClelland

Keyword(s):

Adverse Effect ◽

Gossypium Hirsutum ◽

Fiber Length ◽

Field Experiments ◽

Fiber Strength ◽

Fiber Quality ◽

Growth Stages ◽

Cotton Yield ◽

Seed Cotton ◽

Over The Top

Field experiments were conducted from 1989 through 1991 to determine the effect of DPX-PE350 applied postemergence over-the-top on cotton yield and fiber quality. DPX-PE350, at rates ranging from 50 to 280 g ae ha−1applied to cotton in the VC to R6 growth stages, had no adverse effect on seed cotton yield, micronaire, fiber length, fiber length uniformity, or fiber strength. Cotton injury was 10% or less in all experiments.

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Identification of QTL and QEI for Fiber-related Traits in Gossypium Hirsutum L.

10.21203/rs.3.rs-70074/v1 ◽

2020 ◽

Author(s):

Abdalla Sharkh ◽

Zhonghua Teng ◽

Peng Yang ◽

Junrui Ma ◽

Nsabiyumva Athanase ◽

...

Keyword(s):

Fiber Length ◽

Seed Oil ◽

Agronomic Traits ◽

Fiber Strength ◽

Fiber Quality ◽

Fiber Elongation ◽

Lint Percentage ◽

Gossypium Hirsutum L ◽

Cash Crops ◽

The World

Abstract Background Cotton is one of the most important cash crops in the world, depending on fiber quality, yield, seed oil and protein content. Identifying QTL for fiber related and other agronomic traits will facilitate the genetic improvement in cotton. Results In this study, forty-seven QTL for fiber related traits were identified across four different environments and six of these QTL were detected in more than one environment, including two for lint percentage (qLP-D03-1 and qLP-D09-1), two for fiber length (qFL-A07-2 and qFL-D11-1), and two for fiber micronaire (qFM-A08-1 and qFM-D11-1), respectively. Four QTL clusters contained 12 QTL were distributed on four chromosomes including two in At subgenome and two in the Dt subgenome. Moreover, thirteen QTL by environment interactions (QEI) were recognized, including one for lint percentage, five for fiber length, three for fiber strength, two for fiber micronaire, one for fiber uniformity and one for fiber elongation, respectively. ConclusionSix QTL were detected in more than one environment and two environmentally stable QTL (qLP-D03-1 and qFM-A08-1) interacted significantly environment. The QTL detected in more than one environment could be useful for further fine-mapping and marker assisted selection in cotton.

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Assessment of heterosis based on parental genetic distance estimated with SSR and SNP markers in Upland cotton (Gossypium hirsutum L.)

10.21203/rs.3.rs-65516/v2 ◽

2021 ◽

Author(s):

Xiaoli Geng ◽

Yujie Qu ◽

Yinhua Jia ◽

Shoupu He ◽

Zhaoe Pan ◽

...

Keyword(s):

Fiber Length ◽

Fiber Strength ◽

Fiber Quality ◽

Snp Markers ◽

Fiber Elongation ◽

Elongation Ratio ◽

Lint Percentage ◽

Fiber Quality Traits ◽

Boll Weight ◽

Ssr And Snp Markers

Abstract Background: Heterosis has been extensively utilized in different crops and made a significant contribution to global food security. Genetic distance (GD) is one of the valuable criteria for selecting parents in hybrid breeding. The objectives of this study were to estimate the GD between parents using both simple sequence repeat (SSR) markers and single nucleotide polymorphism (SNP) markers and to investigate the efficiency of the prediction of hybrid performance based on GD. The experiment comprised of four male parents, 282 female parents and 1128 F1, derived from NCII mating scheme. The hybrids, their parents and two check cultivars were evaluated for two years. Performance of F1, mid-parent heterosis (MPH), and best parent heterosis (BPH) were evaluated for ten agronomic and fiber quality traits, including plant height, boll weight, boll number, lint percentage, fiber length, fiber strength, fiber uniformity, fiber elongation ratio, micronaire, and spinning consistent index. Results: Heterosis was observed in all hybrids and, the traits like plant height, boll number, boll weight and lint percentage exhibited higher heterosis than the fiber quality traits. Correlations were significant between parental and F1 performances. The F1 performances between three hybrid sets (Elite×Elite, Exotic×Elite, and Historic×Elite) showed significant differences in eight traits, including boll number, lint percentage, fiber length, fiber strength, fiber uniformity, fiber elongation ratio, micronaire, and spinning consistent index. The correlation of the GD assessed by both SSR and SNP markers was significantly positive. The cluster analysis based on GD results estimated using SNP showed that all the female parents divided into five groups and the F1 performance between these five groups showed significant differences in four traits, including lint percentage, micronaire, fiber strength, and fiber elongation ratio. The correlation between GD and F1 performance, MPH and BPH were significant for lint percentage and micronaire. Conclusions: Our results suggested that GD between parents could be helpful in heterosis prediction for certain traits. This study reveals that molecular marker analysis can serve as a basis for assigning germplasm into heterotic groups and to provide guidelines for parental selection in hybrid cotton breeding.

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Comparison of The Fiber Characteristics of the Some Cotton Varieties Ginned by Using Sawgin and Rollergin

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i6.946-952.3496 ◽

2021 ◽

Vol 9 (6) ◽

pp. 946-952

Author(s):

Sevtap Kartal ◽

Lale Efe

Keyword(s):

Fiber Length ◽

Fiber Strength ◽

Fiber Quality ◽

Block Design ◽

Short Fiber ◽

Consistency Index ◽

Fiber Analysis ◽

Randomized Block Design ◽

Fiber Fineness ◽

Positive Effect

In this study carried out in 2015 under conditions of Kahramanmaraş province of Turkey, it was aimed at determining the effects of sawgin and rollergin methods on fiber quality in some cotton (Gossypium hirsutum L.) cultivars. In the study varieties of Lydia, Carisma, PG 2018, Flash, BA 440, BA 119 Maraş-92 and Erşan-92 were used as experimental materials. The trial was established according to factorial randomized block design with four replications. Seed cottons obtained from the trial were ginned in the rollergin and sawgin machines. In the obtained lint cotton samples, a number of fiber characteristics were determined by using HVI and AFIS fiber analysis devices. Ginnig outturn (38.6%), fiber length (30.21 mm), uniformity index (86.02%), fiber strength (31.76 g tex-1), spinning consistency index (SCI) (104.68) determined by using rollergin system were found higher than ones determined by using sawgin system (respectivelly 37.2%, 29.78 mm, 84.61%, 30.97 g tex-1, 94.50). Short fiber index (3.47%) and nep count (59.40 number g-1) obtained from rollergin system were found lower than ones obtained from sawgin system (respectivelly 4.38% and 119.34 number g-1). As a result it can be said that the rollergin method has positive effect on ginnig outturn, fiber length, uniformity index, fiber strength, spinning consistency index, short fiber index and nep count. When fiber length, fiber strength, spinning consistency index, nep size are considered together the best variety was Lydia cv. (respectivelly 30.87 mm, 32.56 g tex-1, 104.25, 675.63 μm). Ginning outturn, uniformity index, short fiber index, total particule number, dust particule number and trash count are considered together the best variety was Erşan-92 cv. (respectivelly 39.4%, 86.02%, 3.48%, 231.4 number g-1, 206.3 number g-1, 25.13 number g-1). For fiber fineness the best varieties were BA 119 and Maraş-92 cv. (respectivelly 4.78 mic. and 4.80 mic.).

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