scholarly journals Essential Oil Disinfectant Efficacy Against SARS-CoV-2 Microbial Surrogates

2021 ◽  
Vol 9 ◽  
Author(s):  
Emily S. Bailey ◽  
Marina Curcic ◽  
Jnev Biros ◽  
Hüseyin Erdogmuş ◽  
Nurcan Bac ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Essential Oil ◽  
Microbial Contamination ◽  
Effective Means ◽  
Delivery Device ◽  
Oil Blends ◽  
Viral Spread ◽  
Bacterial Indicator ◽  
Disinfectant Efficacy

Reports of COVID-19 cases potentially attributed to fomite transmission led to the extensive use of various disinfectants to control viral spread. Alternative disinfectants, such as essential oils, have emerged as a potential antimicrobial. Four essential oil blends were tested on three different surfaces inoculated with a coronavirus surrogate, bacteriophage Phi 6, and a bacterial indicator, Staphylococcus aureus. Log10 concentration reductions were analyzed using GraphPad Prism software. Data collected in this study show that the application of dilute essential oil disinfectants using a spray delivery device is an effective way to reduce concentrations of bacterial and viral microorganisms on ceramic, stainless steel, and laminate surfaces. Surrogate viruses were reduced up to 6 log10 PFU and bacterial were reduced up to 4 log10 CFU. Although surfaces are no longer considered a high risk fomite for COVID-19 transmission, the disinfection of microorganisms on surfaces remains an important consideration for high touch areas in hospitals, waiting rooms, etc. The application of spray disinfectants, based on essential oil blends, provides a rapid and effective means to reduce microbial contamination on high-touched surfaces.

Bacterial Numbers on Penicylinders Used in Disinfectant Testing: Use of 24 Hour Adjusted Broth Cultures

1988 ◽  
Vol 71 (1) ◽  
pp. 9-11
Author(s):  
Eugene C Cole ◽  
William A Rutala
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Bacterial Attachment ◽  
Broth Culture ◽  
Relative Efficacy ◽  
Salmonella Choleraesuis ◽  
Growth Studies ◽  
Cell Densities ◽  
Disinfectant Efficacy

Abstract The current AOAC use-dilution methods of disinfectant efficacy testing require the use of 48-54 h unadjusted broth cultures of Salmonella choleraesuis, Staphylococcus aureus, and Pseudomonas aeruginosa for the inoculation of stainless steel penicylinders. The use of unadjusted broth cultures contributes to noncomparable numbers of organisms on penicylinders among the test strains due to relative efficacy of bacterial attachment to penicylinders and to bacterial numbers in broth. To achieve comparable numbers of cells on the penicylinders among the 3 test strains, the cell densities of S. aureus and P. aeruginosa in broth culture were visually adjusted. Growth studies were conducted using S. choleraesuis and P. aeruginosa to determine the numbers of cells in broth at timed intervals and the corresponding numbers of cells attaching to the penicylinders. Results showed that the use of the 24 h broth cultures for all 3 test strains, with adjustment of S. aureus and P. aeruginosa broths, contributes to more comparable numbers of organisms attached to the penicylinders used in disinfectant testing.

Efficacy of using oregano essential oil and carvacrol to remove young and mature Staphylococcus aureus biofilms on food-contact surfaces of stainless steel

LWT ◽  
2018 ◽  
Vol 93 ◽  
pp. 293-299 ◽  
Author(s):  
Jessica Bezerra dos Santos Rodrigues ◽  
Neyrijane Targino de Souza ◽  
João Otavio Alcântara Scarano ◽  
Janaína Maria de Sousa ◽  
Myrella Cariry Lira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Essential Oil ◽  
Oregano Essential Oil ◽  
Food Contact ◽  
Food Contact Surfaces ◽  
Contact Surfaces

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

scholarly journals Heat transfer analysis of Staphylococcus aureus on stainless steel with microwave radiation

1999 ◽  
Vol 87 (3) ◽  
pp. 396-401 ◽  
Author(s):  
C. B. A. Yeo ◽  
I. A. Watson ◽  
D. E. S. Stewart-Tull ◽  
V. H. H. Koh
Keyword(s):  
Heat Transfer ◽  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Microwave Radiation ◽  
Heat Transfer Analysis

Antimicrobial activity of free and liposome-encapsulated thymol and carvacrol against Salmonella and Staphylococcus aureus adhered to stainless steel

2017 ◽  
Vol 252 ◽  
pp. 18-23 ◽  
Author(s):  
Juliana Both Engel ◽  
Caroline Heckler ◽  
Eduardo Cesar Tondo ◽  
Daniel Joner Daroit ◽  
Patrícia da Silva Malheiros
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Stainless Steel ◽  
And Staphylococcus Aureus

The effect of inoculating the bovine teat duct with small numbers of Staphylococcus aureus

1965 ◽  
Vol 32 (2) ◽  
pp. 171-179 ◽  
Author(s):  
F. H. S. Newbould ◽  
F. K. Neave
Keyword(s):  
Staphylococcus Aureus ◽  
Stainless Steel ◽  
Post Inoculation ◽  
Intramammary Infection ◽  
Colony Forming Units ◽  
Previous Infection ◽  
Glass Rods

SummaryFifty-seven inoculations of a strain of Staphylococcus aureus were made into the outer 4 mm of the teat ducts of 8 cows. The inocula ranged from 10 to 600 colony-forming units (CFU) and were made with a stainless steel instrument designed to overcome the disadvantages of glass rods and cotton swabs hitherto used.A single inoculation resulted in either no colonization of the duct, in temporary colonization for up to 7 days, or in colonization followed by intramammary infection.The recovery of the organisms depended on the size of the inoculum. When 70–100 CFU were placed in the teat duct no organisms were recovered from 12 of 24 quarters after the 1st post-inoculation milking. When 500–600 CFU were used, organisms were recovered from all of 31 quarters for at least 3 milkings, and from 61% for 6 milkings or more, in spite of dipping the teats in a strong disinfectant twice daily.Intramammary infection developed in 1 of 12 quarters (8%) inoculated in the teat duct with about 600 CFU when the animals were milked twice daily, and in 5 of 19 (23%) quarters if the 1st post-inoculation milking was omitted.There was no evidence of sensitization resulting from previous infection.

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