Effects of Mesobiliverdin IXα-Enriched Microalgae Feed on Gut Health and Microbiota of Broilers

Gut inflammatory bowel diseases (IBDs) links to animal medicinal feed and antibiotic-resistance are fueling major economic impacts in the agricultural livestock industry. New animal feeds that promote livestock gut health and control of IBDs without antibiotics are needed. This study investigates the effects of mesobiliverdin IXα (MBV)-enriched microalgae spirulina extracts on the growth performance, blood parameters, intestinal morphology, and gut microbiota of broilers. A total of 288 1-day-old broiler chicks (Arbor Acres) were randomly allotted to six dietary treatments (4 pens/treatment and 12 birds/pen). The dietary treatments comprised a basal diet as control (CON), basal diet plus 0.05 and 0.1% microalgae extract as low and high dose, respectively (SP1 and SP2), basal diet plus 0.05 and 0.1% MBV-enriched microalgae extract as low and high dose, respectively (MBV-SP1 and MBV-SP2), and basal diet plus 0.1% amoxicillin (AMX). All treated animals showed no significant differences in live weight, average daily gain, and feed efficiency compared to control animals. Histological examination showed that AMX treatment decreased the villi lengths of the duodenum and ileum below control villi length (P < 0.05) while MBV-SP1 and particularly MBV-SP2 increased villi lengths in the duodenum, jejunum, and ileum above AMX -treatment lengths (P < 0.05). The Firmicutes/Bacteroidetes ratio increased in the cecum of broilers fed AMX (P < 0.05) while SP2, MBV-SP1, and MBV-SP2-fed animals showed (in order) increasing ratios up to the AMX level. The abundance of bacterial species of the genus Lactobacillus increased in MBV-SP1 and MBV-SP2-fed groups including a striking increase in Lactobacillus salivarius abundance with MBV-SP2 (P < 0.05). Feeding MBV-SP1 and MBV-SP2 decreased the level of pro-inflammatory cytokine IL-6 in plasma of broilers to a greater extent than SP1 and SP2. These results reveal that MBV-enriched microalgae extracts improve the intestinal health and beneficial microflora composition of broilers.

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Effect of seaweed-derived laminarin and fucoidan and zinc oxide on gut morphology, nutrient transporters, nutrient digestibility, growth performance and selected microbial populations in weaned pigs

British Journal Of Nutrition ◽

10.1017/s0007114513004224 ◽

2014 ◽

Vol 111 (9) ◽

pp. 1577-1585 ◽

Cited By ~ 36

Author(s):

G. Heim ◽

A. M. Walsh ◽

T. Sweeney ◽

D. N. Doyle ◽

C. J. O'Shea ◽

...

Keyword(s):

Growth Performance ◽

Average Daily Gain ◽

Live Weight ◽

Basal Diet ◽

Nutrient Digestibility ◽

Gut Health ◽

Gross Energy ◽

Weaning Age ◽

Nutrient Transporter ◽

Dietary Treatments

In the present study, two experiments were conducted to (1) evaluate the effect of laminarin and/or fucoidan on ileal morphology, nutrient transporter gene expression and coefficient of total tract apparent digestibility (CTTAD) of nutrients and (2) determine whether laminarin inclusion could be used as an alternative to ZnO supplementation in weaned pig diets. Expt 1 was designed as a 2 × 2 factorial arrangement, comprising four dietary treatments (n 7 replicates, weaning age 24 d, live weight 6·9 kg). The dietary treatments were as follows: (1) basal diet; (2) basal diet+300 ppm laminarin; (3) basal diet+240 ppm fucoidan; (4) basal diet+300 ppm laminarin and 240 ppm fucoidan. There was an interaction between laminarin and fucoidan on the CTTAD of gross energy (GE) (P< 0·05) and the expression of sodium–glucose-linked transporter 1 (SGLT1/SLC5A1) and GLUT1/SLC2A1 and GLUT2/SLC2A2 (P< 0·05) in the ileum. The laminarin diet increased the CTTAD of GE and increased the expression of SGLT1, GLUT1 and GLUT2 compared with the basal diet. However, there was no effect of laminarin supplementation on these variables when combined with fucoidan. Expt 2 was designed as a complete randomised design (n 8 replicates/treatment, weaning age 24 d, live weight 7·0 kg), and the treatments were (1) basal diet, (2) basal diet and laminarin (300 ppm), and (3) basal diet and ZnO (3100 ppm, 0–14 d, and 2600 ppm, 15–32 d post-weaning). The laminarin diet increased average daily gain and gain:feed ratio compared with the basal diet during days 0–32 post-weaning (P< 0·01) and had an effect similar to the ZnO diet. These results demonstrate that laminarin provides a dietary means to improve gut health and growth performance post-weaning.

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Effects of prebiotics on intestinal histo-morphometry and gut microflora status of broiler chickens

Indian Journal of Animal Research ◽

10.18805/ijar.b-3579 ◽

2018 ◽

Author(s):

A. Biswas ◽

R. Messam ◽

M. Kumawat ◽

M. Namit ◽

A.B. Mandal ◽

...

Keyword(s):

Broiler Chickens ◽

Dietary Treatment ◽

Basal Diet ◽

Feed Additive ◽

Broiler Chicks ◽

Growth Promoter ◽

Gut Health ◽

Treatment Groups ◽

Dietary Treatments ◽

Soybean Diet

A feeding trial was conducted to assess the effect of prebiotics on intestinal histo-morphometry and gut health status of broiler chickens. A total number of 240 day-old broiler chicks were randomly assigned to six dietary treatment groups having five replicates each with eight birds per replicate for six weeks. A basal corn-soybean diet was formulated that served as control (T1). The rest five test dietary treatments were prepared by adding antibiotic (bacitracin methylene di-salicylate-BMD at 0.002% of diet (T2), prebiotics i.e., mannan oligosaccharides (MOS) at 0.1% or 0.2% (T3 and T4) and fructo oligosaccharides (FOS) at 0.1% or 0.2% (T5, T6 respectively) with basal diet. Total anaerobes counts were significantly (P LESS THAN 0.05) reduced at 21 and 42 d at ileum in T2, T3 and T4 groups whereas, coliforms count were reduced (P LESS THAN 0.05) in T2 and T3 groups. However, lactic acid bacteria (LAB) count were significantly (P Less than 0.05) increased in T3 and T4 groups but lactobacillus count were increased (P less than 0.05) in only T3 group at 21 and 42 d in ileum. Villus height (VH) and width (VW) were significantly increased (P less than 0.05) at 21d and 42 d in T3 and T4 groups, whereas, at 21 d, crypt depth (CD) were increased (P less than 0.05) in T3 and T4 groups but at 42 d, CD were significantly improved in T3 and T4 or T5 and T6 groups. No significant (P>0.05) differences were observed in ratio between VH and CD at 21d or 42 d. Thus, it might he concluded that 0.1% or 0.2% MOS inclusion in basal diet improved intestinal histo-morphometry and gut health in broiler chickens and it is a better alternate feed additive to replacement of antibiotic growth promoter.

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Maintaining the same ratio between standardized ileal digestible methionine and cysteine do not affect its requirements for starting pigs

The Journal of Agricultural Science ◽

10.1017/s0021859621000587 ◽

2021 ◽

pp. 1-9

Author(s):

C. F. Muniz ◽

L. D. Castilha ◽

M. S. S. Pozza ◽

C. A. L. Oliveira ◽

F. Sato ◽

...

Keyword(s):

Block Design ◽

Average Daily Gain ◽

Daily Intake ◽

Live Weight ◽

Basal Diet ◽

Blood Parameters ◽

Longissimus Dorsi ◽

Randomized Block Design ◽

Sulphur Amino Acids ◽

Daily Gain

Abstract This study aimed to determine the requirements of standardized ileal digestible (SID) methionine (Met) + cysteine (Cys) of 15–30 kg barrows, maintaining or increasing the 1:1 ratio between SID Met and SID Cys. Seventy crossbred barrows averaging 15.2 ± 0.54 kg of live weight were allotted in a randomized block design 2 × 3 + 1 factorial scheme, with five replicates and two animals per pen. Treatments consisted of maintaining or increasing the 1:1 ratio between SID Met and SID Cys, three levels of SID Met + Cys (5.8, 6.4 and 7.0 g/kg) and a basal diet containing the lowest SID Met + Cys level (5.2 g/kg), formulated to provide a 1:1 ratio of SID Met (2.6 g/kg) and SID Cys (2.6 g/kg). Performance, blood parameters, longissimus dorsi muscle depth, backfat thickness and S–S linkages in the bristles were evaluated. The best average daily gain was estimated at 6.61 g/kg of SID Met + Cys, without maintaining the 1:1 ratio between sulphur amino acids (SAA). The daily intake of SID Met + Cys increased due to dietary SID Met + Cys levels, maintaining or increasing the same ratio between SAA. Plasma glucose increased and total cholesterol decreased according to SID Met + Cys levels, without maintaining the same ratio between the SAA. The requirement of SID Met + Cys for 15–30 kg barrows was 6.61 g/kg (7.88 g/day) for an optimum average daily gain, with no need to maintain the same ratio between the SAA.

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Modified Black Soldier Fly Larva Fat in Broiler Diet: Effects on Performance, Carcass Traits, Blood Parameters, Histomorphological Features and Gut Microbiota

Animals ◽

10.3390/ani11061837 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1837

Author(s):

Sihem Dabbou ◽

Angelo Lauwaerts ◽

Ilario Ferrocino ◽

Ilaria Biasato ◽

Federico Sirri ◽

...

Keyword(s):

Broiler Chickens ◽

Basal Diet ◽

Blood Parameters ◽

Fecal Microbiota ◽

Chain Fatty Acid ◽

Intestinal Morphology ◽

Control Group ◽

Soy Oil ◽

Slaughter Performance ◽

Dietary Treatments

In this study, a total of 200 male broiler chickens (Ross 308) were assigned to four dietary treatments (5 pens/treatment and 10 birds/pen) for two feeding phases: starter (0–11 days of age) and grower-finisher (11–33 days of age). A basal diet containing soy oil (SO) as added fat was used as control group (C), tested against three experimental diets where the SO was partially substituted by BSF larvae fat (BSF) or one of two types of modified BSF larvae fat (MBSF1 and MBSF2, respectively). The two modified BSF larvae fats had a high and low ratio of monobutyrin to monoglycerides of medium chain fatty acid, respectively. Diet did not influence the growth or slaughter performance, pH, color, or the chemical composition of breast and thigh muscles, gut morphometric indices, or histopathological alterations in all the organs. As far as fecal microbiota are concerned, MBSF1 and MBSF2 diets reduced the presence of Clostridium and Corynebacterium, which can frequently cause infection in poultry. In conclusion, modified BSF larva fat may positively modulate the fecal microbiota of broiler chickens without influencing the growth performance and intestinal morphology or showing any adverse histopathological alternations.

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Effect of Dietary Inulin Supplementation on Growth Performance, Carcass Traits, and Meat Quality in Growing–Finishing Pigs

Animals ◽

10.3390/ani9100840 ◽

2019 ◽

Vol 9 (10) ◽

pp. 840 ◽

Cited By ~ 2

Author(s):

Weikang Wang ◽

Daiwen Chen ◽

Bing Yu ◽

Zhiqing Huang ◽

Yuheng Luo ◽

...

Keyword(s):

Growth Performance ◽

Meat Quality ◽

Control Diet ◽

Average Daily Gain ◽

Carcass Traits ◽

Basal Diet ◽

Expression Level ◽

Finishing Pigs ◽

Gut Health ◽

Dietary Treatments

Inulin is one of the commercially feasible dietary fibers that has been implicated in regulating the gut health and metabolism of animals. This experiment was conducted to investigate the effect of dietary inulin supplementation on growth performance and meat quality in growing–finishing pigs. Thirty-six Duroc × Landrace × Yorkshire White growing barrows (22.0 ± 1.0 kg) were randomly allocated to two dietary treatments consisting of a basal control diet (CON) or basal diet supplemented with 0.5% inulin (INU). Results showed that inulin supplementation tended to increase the average daily gain (ADG) at the fattening stage (0.05 < p < 0.10). Inulin significantly increased the dressing percentage (p < 0.05) and tended to increase the loin-eye area. The serum concentrations of insulin and IGF-I were significantly higher (p < 0.05) in the INU group than in the CON group. Moreover, inulin supplementation significantly elevated the expression level of myosin heavy chain II b (MyHC IIb) in the longissimus dorsi (p < 0.05). Inulin significantly upregulated the expression of mammalian rapamycin target protein (mTOR) but decreased (p < 0.05) the expression level of muscle-specific ubiquitin ligase MuRF-1. These results show the beneficial effect of inulin supplementation on the growth performance and carcass traits in growing–finishing pigs, and will also facilitate the application of inulin in swine production.

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Live Yeast or Live Yeast Combined with Zinc Oxide Enhanced Growth Performance, Antioxidative Capacity, Immunoglobulins and Gut Health in Nursery Pigs

Animals ◽

10.3390/ani11061626 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1626

Author(s):

Shenfei Long ◽

Tengfei He ◽

Sung Woo Kim ◽

Qinghui Shang ◽

Tadele Kiros ◽

...

Keyword(s):

Average Daily Gain ◽

Basal Diet ◽

Conversion Ratio ◽

Gut Health ◽

Total Volatile ◽

Feed Conversion ◽

Nursery Pigs ◽

Serum Iga ◽

Daily Gain ◽

Live Yeast

This study aimed to investigate the effects of dietary LY or LY combined with ZnO supplementation on performance and gut health in nursery pigs. 192 Duroc × Landrace × Yorkshire piglets (weaned on d 32 of the age with 9.2 ± 1.7 kg BW) were allocated into four treatments with eight replicate pens, six piglets per pen. The treatments included a basal diet as control (CTR), an antibiotic plus ZnO diet (CTC-ZnO, basal diet + 75 mg/kg of chlortetracycline + ZnO (2000 mg/kg from d 1 to 14, 160 mg/kg from d 15 to 28)), a LY diet (LY, basal diet + 2 g/kg LY), and a LY plus ZnO diet (LY-ZnO, basal diet + 1 g/kg LY + ZnO). The results showed that pigs fed LY or LY-ZnO had increased (p < 0.05) average daily gain, serum IgA, IgG, superoxide dismutase, fecal butyric acid, and total volatile fatty acid concentrations, as well as decreased (p < 0.05) feed conversion ratio and diarrhea rate compared with CTR. In conclusion, pigs fed diets with LY or LY combined with ZnO had similar improvement to the use of antibiotics and ZnO in performance, antioxidant status, immunoglobulins, and gut health in nursery pigs.

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Effect of supplementary Aspergillus niger phytase in diets for piglets on their performance and apparent digestibility of phosphorus

Animal Science ◽

10.1017/s0003356100021127 ◽

1992 ◽

Vol 55 (3) ◽

pp. 425-430 ◽

Cited By ~ 32

Author(s):

S. Beers ◽

A. W. Jongbloed

Keyword(s):

Aspergillus Niger ◽

Average Daily Gain ◽

Live Weight ◽

Basal Diet ◽

Apparent Digestibility ◽

Feeding Trial ◽

Daily Food ◽

Microbial Phytase ◽

Daily Gain ◽

Aspergillus Niger Phytase

AbstractA total of 384 piglets from a three-way cross Yorkshire ♂ × (Finnish Landrace ♂ × Dutch Landrace♀) ♀ in the live-weight range of 11 to 25 kg were used in a feeding trial. The effect of supplementary microbial phytase from Aspergillus niger var. Van Tieghem on performance (average daily gain (ADG), average daily food intake (ADFI) and food conversion ratio (FCR)) and apparent digestibility of total phosphorus (P), in comparison with a non-supplemented basal diet or a diet supplemented with one or two levels of monocalcium phosphate (MCP), were studied.The piglets were allotted to 12 blocks. Within each block, four experimental diets were offered ad libitum for a period of 4 weeks i.e. (1) basal diet; (2) basal diet + 1·0 g P, from MCP per kg diet; (3) basal diet + 1·9 g P, from MCP per kg diet; and (4) basal diet + 1450 phytase units (PTU) per kg diet. The diets, with added chromium III oxide as a marker, were cold pelleted without steam at a temperature not above 60°C to avoid a possible inactivation of the phytase. In all diets the ratio calcium: digestible P was intended to be constant.Adding 1450 PTU of Aspergillus niger phytase enhanced the apparent digestibility of P by proportionately 0·216 when compared with diet 1 and by proportionately 0·100 compared with diet 3. In addition, significantly higher ADG and ADFI and better FCR were noted in pigs receiving supplementary microbial phytase than in pigs on any other treatment. The content of digestible P of the diet with added phytase, however, zvas between the two levels of the diets with added MCP.

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Quality of the carcass and organs of chicken fed with two different insects meals

Journal of Insects as Food and Feed ◽

10.3920/jiff2017.0079 ◽

2018 ◽

Vol 4 (4) ◽

pp. 269-274

Author(s):

M.I. Amobi ◽

C.I. Ebenebe

Keyword(s):

Broiler Chickens ◽

Live Weight ◽

Organ Weight ◽

Poultry Feed ◽

Source Control ◽

Carcass Quality ◽

Broiler Chicks ◽

Major Protein ◽

Feed Formulation ◽

Dietary Treatments

The influence of insect protein origins on broiler chicken meat parameters were studied using 135 day old Arbor acre birds subjected to three dietary treatments: Diet A comprised of a standard feed of the brand name ‘vital feed’ containing fishmeal as the major protein source (control), while Diet B and Diet C contained insect meal from African palm weevil larvae (Rhynchophorus phoenicis), respectively, winged termite (Macrotermes bellicosus) as replacement for fishmeal. Forty five broiler chicks were randomly assigned to each of the dietary treatments at the rate of fifteen chicks per replicate making three replicates per treatment in a completely randomised design. The experiment lasted for eight weeks. During this period, the birds were housed in similar cages and subjected to similar husbandry and sanitation practices, such that the only source of variation was the dietary treatments. After eight weeks of the experiment, carcass quality and organ weight measurements were analysed using appropriate statistical analysis. The result obtained showed that the broiler chickens fed insect meals performed significantly (P<0.05) better in terms of live weight gain (2,200.85±64.23 g and 2,046±58.40 g for Diet B and Diet C) compared to control. Carcass quality relating to defeathered weight and eviscerated weight also followed a similar trend with Diet B (2,187.74±62.72 g and 1,788.93±70.70 g), Diet C (2,015.83±74.62 g and 1,466.97±100.05 g), and Diet A (control; 1,526.47±47.40 g and 1,240.30±64.53 g). The records of cut-up parts and organ weight measurements were also significantly different (P<0.05) for the birds on insect meal (Diet B and Diet C) than those on fishmeal based diet (Diet A). The result showed that insect meal can effectively serve as a major source of protein in poultry feed formulation.

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Effects of inorganic minerals replaced by complexed glycinates on growth, blood profiles, immune responses, intestinal morphology, and mineral excretion in piglets

10.21203/rs.3.rs-35495/v1 ◽

2020 ◽

Author(s):

Xun Pei ◽

Geng Wang ◽

Lujie Liu ◽

Wanjing Sun ◽

Zhiping Xiao ◽

...

Keyword(s):

Immune Responses ◽

Low Dose ◽

Average Daily Gain ◽

Basal Diet ◽

Intestinal Morphology ◽

Trace Minerals ◽

Control Group ◽

Trace Mineral ◽

Mineral Excretion ◽

Blood Profiles

Abstract BACKGROUND: The effects of inorganic trace minerals (ITM) replaced by low-dose glycine-complexed trace minerals (GCM) on growth, serum parameters, immunity, intestinal morphology, and mineral excretion in piglets were investigated. One hundred and twenty-eight weaned piglets (14.18 ± 0.33kg body weight (BW)) were randomly assigned to 4 treatments with 4 replicates, 8 piglets per replicate. Treatments consist of: (T1) basal diet + 100% inorganic trace mineral (ITM) as the control group (20 ppm Cu, 150 ppm Fe, 150 ppm Zn, and 30 ppm Mn from sulfates); (T2) basal diet + 50% ITM (Cu, Fe, Zn, and Mn from sulfates, 50% of control) + 50% organic trace minerals (OTM, Cu, Fe, Zn, and Mn from glycine complexed trace minerals (GCM), 50% of control); (T3) basis diet + 50% OTM from GCM; (T4) basal diet + 70% OTM from GCM. The feeding period lasted 28 d and was divided into 2 stages (0 to 14 d and 15 to 28 d). After feeding trial , 6 pigs per treatment were randomly selected to slaughter for sampling. RESULTS: Average daily gain, feed intake, and G:F were not affected by dietary treatments during the overall period. During the second, and the overall feeding phases, the digestibility of Zn and Fe in T3 and T4 was higher than that of T1 ( P < 0.05). The concentration of serum ferritin in T2 was significantly higher than T3 and T4. Serum immunoglobulin A concentration in the ileal mucosa of T2 was higher than that of T1 ( P < 0.05), and the higher duodenum villus height was observed in T4 compared with the rest treatments ( P < 0.05). The lowest trace mineral excretion was overserved in T3 ( P < 0.01); in addition, the urinary concentrations of Zn and Fe in T2 were lower than that in T1 ( P < 0.05). CONCLUSION: These results indicate that GCM have higher bioavailability than ITM, and that supplementation of low-dose GCM to replace full dose ITM could reduce mineral excretion without affecting performance, blood profiles, immune responses, and intestinal morphology in piglets.

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Effects of Dietary Taurine Supplementation to Gilts during Late Gestation and Lactation on Offspring Growth and Oxidative Stress

Animals ◽

10.3390/ani9050220 ◽

2019 ◽

Vol 9 (5) ◽

pp. 220 ◽

Cited By ~ 2

Author(s):

Mengmeng Xu ◽

Long Che ◽

Kaiguo Gao ◽

Li Wang ◽

Xuefen Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Protein Expression ◽

Growth Performance ◽

Barrier Function ◽

Average Daily Gain ◽

Basal Diet ◽

Intestinal Morphology ◽

Late Gestation ◽

Villus Height ◽

And Oxidative Stress

Birth is one of the most important events of animal production agriculture, as newborns are abruptly forced to adapt to environmental and nutritional disruptions that can lead to oxidative damage and delay in growth. Taurine (Tau) is an important regulator of oxidative stress and possesses growth-enhancing properties. In the present study, we investigated the effects of dietary Tau supplementation in gilts during late gestation and lactation on the growth performance of piglets by assessing intestinal morphology and barrier function, and oxidative stress status. Sixteen gilts were randomly allocated to the Con (basal diet) and Tau (basal diet with 1% Tau) groups from 75 d of gestation to weaning. Maternal dietary Tau supplementation significantly increased weaning weight and average daily gain weight in piglets. Piglets in the Tau group had higher villus height and villus height-to-crypt depth ratio (VCR), ZO-1 protein expression, and secretory immunoglobulin A (sIgA) content in the jejunum. Meanwhile, Tau bebeficial affected the milk quality of gilts, as indicated by decreased malondialdehyde (MDA) concentration and increased total superoxide dismutase (T-SOD), total antioxidative capability (T-AOC), glutathione peroxidase (GPx), and catalase (CAT) activity. Furthermore, Tau supplementation increased T-SOD activity in plasma and SOD2 protein expression in the jejunum in the piglets. In conclusion, this study provides evidence that dietary Tau supplementation to gilts improves growth performance in piglets, owing to improved intestinal morphology and barrier function, as well as inhibition of oxidative stress.

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