scholarly journals Transcriptomic Analysis of Short/Branched-Chain Acyl-Coenzyme a Dehydrogenase Knocked Out bMECs Revealed Its Regulatory Effect on Lipid Metabolism

2021 ◽  
Vol 8 ◽  
Author(s):  
Ping Jiang ◽  
Ambreen Iqbal ◽  
Mengyan Wang ◽  
Xiaohui Li ◽  
Xibi Fang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Fatty Acid Metabolism ◽  
Milk Fat ◽  
Acid Metabolism ◽  
Chain Acyl ◽  
Fat Synthesis ◽  
Branched Chain ◽  
Go Terms ◽  
Milk Fat Synthesis

The acyl-CoA dehydrogenase family of enzymes includes short/branched-chain acyl-CoA dehydrogenase (ACADSB), which catalyzes the dehydrogenation of acyl-CoA derivatives in fatty acid metabolism. Our previous findings suggested that ACADSB was a critical candidate gene affecting milk fat synthesis by comparing the transcriptome in bovine mammary epithelial cells (bMECs) from Chinese Holstein dairy cows producing high-fat and low-fat milk as well as gene functional validation studies on the cellular level. In the present study, ACADSB in bMECs was knocked out (KO) using a CRISPR/Cas9 system, and mRNA transcriptome was further sequenced to verify the function of the ACADSB gene and analyze its correlation with lipid metabolism. The findings revealed that 15,693 genes were expressed, 1,548 genes were differentially expressed genes (DEGs), and 6,098 GO terms were enriched, of which 637 GO terms were greatly enhanced, such as phospholipid-translocation ATPase activity (GO:0004012), lipoprotein lipase activity (GO:0004465), acyl-CoA desaturase activity (GO:0016215), and so on. The analysis by KEGG showed that DEGs were distributed over 247 pathogens, of which 49 were significantly enriched, including the metabolism of fatty acids (PATH: 01212), metabolism of glycerolipid (PATH: 00561), and signaling of adipocytokines (PATH: 04920). The CHOL, TGs and FFA contents in bMECs were reduced when the ACADSB gene was knocked out. The RT2 Profiler PCR array also revealed that the loss of the ACADSB gene changed the expression levels of functional genes involved in lipid metabolism, including ACADL, ACOX2, ACAT2, and FABP3. In conclusion, the current findings show that ACADSB is a key regulator of lipid metabolism in bMECs. The ACADSB−/− bMECs could also be useful genetic material and tools for future research into gene functions related to lipid and fatty acid metabolism. It will be valuable for revealing the gene regulatory roles and molecular mechanisms in milk fat synthesis.

miR-497 regulates fatty acid synthesis via LATS2 in bovine mammary epithelial cells

2020 ◽  
Vol 11 (10) ◽  
pp. 8625-8636
Author(s):  
Zhi Chen ◽  
Shuangfeng Chu ◽  
Yusheng Liang ◽  
Tianle Xu ◽  
Yujia Sun ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Fatty Acid Synthesis ◽  
Milk Fat ◽  
Acid Metabolism ◽  
Mammary Epithelial Cells ◽  
Acid Synthesis ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Fat Synthesis

Both mRNA and miRNA play an important role in the regulation of mammary fatty acid metabolism and milk fat synthesis.

Human Very-Long-Chain Acyl-CoA Synthetase: Cloning, Topography, and Relevance to Branched-Chain Fatty Acid Metabolism

1999 ◽  
Vol 257 (2) ◽  
pp. 615-621 ◽  
Author(s):  
Steven J. Steinberg ◽  
Susan J. Wang ◽  
Do G. Kim ◽  
Stephanie J. Mihalik ◽  
Paul A. Watkins
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Chain Fatty Acid ◽  
Chain Acyl ◽  
Branched Chain ◽  
Long Chain

scholarly journals Identification of differentially expressed genes and pathways between intramuscular and abdominal fat-derived preadipocyte differentiation of chickens in vitro

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Meng Zhang ◽  
Fang Li ◽  
Xiang-fei Ma ◽  
Wen-ting Li ◽  
Rui-rui Jiang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Differentially Expressed Genes ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Adipogenic Differentiation ◽  
Abdominal Fat ◽  
Receptor Interaction ◽  
Preadipocyte Differentiation ◽  
Factors Affecting

Abstract Background The distribution and deposition of fat tissue in different parts of the body are the key factors affecting the carcass quality and meat flavour of chickens. Intramuscular fat (IMF) content is an important factor associated with meat quality, while abdominal fat (AbF) is regarded as one of the main factors affecting poultry slaughter efficiency. To investigate the differentially expressed genes (DEGs) and molecular regulatory mechanisms related to adipogenic differentiation between IMF- and AbF-derived preadipocytes, we analysed the mRNA expression profiles in preadipocytes (0d, Pre-) and adipocytes (10d, Ad-) from IMF and AbF of Gushi chickens. Results AbF-derived preadipocytes exhibited a higher adipogenic differentiation ability (96.4% + 0.6) than IMF-derived preadipocytes (86.0% + 0.4) (p < 0.01). By Ribo-Zero RNA sequencing, we obtained 4403 (2055 upregulated and 2348 downregulated) and 4693 (2797 upregulated and 1896 downregulated) DEGs between preadipocytes and adipocytes in the IMF and Ad groups, respectively. For IMF-derived preadipocyte differentiation, pathways related to the PPAR signalling pathway, ECM-receptor interaction and focal adhesion pathway were significantly enriched. For AbF-derived preadipocyte differentiation, the steroid biosynthesis pathways, calcium signaling pathway and ECM-receptor interaction pathway were significantly enriched. A large number of DEGs related to lipid metabolism, fatty acid metabolism and preadipocyte differentiation, such as PPARG, ACSBG2, FABP4, FASN, APOA1 and INSIG1, were identified in our study. Conclusion This study revealed large transcriptomic differences between IMF- and AbF-derived preadipocyte differentiation. A large number of DEGs and transcription factors that were closely related to fatty acid metabolism, lipid metabolism and preadipocyte differentiation were identified in the present study. Additionally, the microenvironment of IMF- and AbF-derived preadipocyte may play a significant role in adipogenic differentiation. This study provides valuable evidence to understand the molecular mechanisms underlying adipogenesis and fat deposition in chickens.

scholarly journals Bta-miR-34b regulates milk fat biosynthesis by targeting mRNA decapping enzyme 1A (DCP1A) in cultured bovine mammary epithelial cells1

2019 ◽  
Vol 97 (9) ◽  
pp. 3823-3831 ◽  
Author(s):  
Yujuan Wang ◽  
Wenli Guo ◽  
Keqiong Tang ◽  
Yaning Wang ◽  
Linsen Zan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Droplet ◽  
Milk Fat ◽  
Fatty Acid Synthase ◽  
Binding Protein ◽  
Mammary Epithelial ◽  
Luciferase Reporter ◽  
Fatty Acid Binding ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Abstract Milk fat is a main nutritional component of milk, and it has become one of the important traits of dairy cow breeding. Recently, there is increasing evidence that microRNAs (miRNA) play significant roles in the process of milk fat synthesis in the mammary gland. Primary bovine mammary epithelial cells (BMEC) were harvested from midlactation cows and cultured in DMEM/F-12 medium with 10% fetal bovine serum, 100 units/mL penicillin, 100 µg/mL streptomycin, 5 µg/mL bovine insulin, 1 µg/mL hydrocortisone, and 2 µg/mL bovine prolactin. We found that miR-34b mimic transfection in BMEC reduced the content of intracellular triacylglycerol (TAG) and lipid droplet accumulation via triacylglycerol assay and Oil Red O staining; meanwhile, overexpression of miR-34b inhibited mRNA expression of lipid metabolism-related genes such as peroxisome proliferator-activated receptor gamma (PPARγ), fatty acid synthase (FASN), fatty acid binding protein 4 (FABP4), and CCAAT enhancer binding protein alpha (C/EBPα). Whereas miR-34b inhibitor resulted in completely opposite results. Furthermore, q-PCR and western blot analysis revealed the mRNA and protein expression levels of DCP1A were downregulated in miR-34b mimic transfection group and upregulated in miR-34b inhibitor group. Moreover, luciferase reporter assays verified that DCP1A was the direct target of miR-34b and DCP1A gene silencing in BMEC-inhibited TAG accumulation and suppressed lipid droplet formation. In conclusion, these findings revealed a novel miR-34b–DCP1A axis that has a significant role in regulating milk fat synthesis and suggested that miR-34b may be used to improve the beneficial ingredients in milk.

scholarly journals Overexpression of Rat Long Chain Acyl-CoA Synthetase 1 Alters Fatty Acid Metabolism in Rat Primary Hepatocytes

2006 ◽  
Vol 281 (48) ◽  
pp. 37246-37255 ◽  
Author(s):  
Lei O. Li ◽  
Douglas G. Mashek ◽  
Jie An ◽  
Scott D. Doughman ◽  
Christopher B. Newgard ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Primary Hepatocytes ◽  
Chain Acyl ◽  
Rat Primary Hepatocytes ◽  
Long Chain

scholarly journals Lipid Metabolism Profiles in Rheumatic Diseases

2021 ◽  
Vol 12 ◽  
Author(s):  
Weilin Chen ◽  
Qi Wang ◽  
Bin Zhou ◽  
Lihua Zhang ◽  
Honglin Zhu
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Rheumatic Diseases ◽  
Fatty Acid Metabolism ◽  
High Mortality ◽  
Acid Metabolism ◽  
Cell Metabolism ◽  
Therapeutic Strategies ◽  
Clinical Applications ◽  
Multiple Organs

Rheumatic diseases are a group of chronic autoimmune disorders that involve multiple organs or systems and have high mortality. The mechanisms of these diseases are still ill-defined, and targeted therapeutic strategies are still challenging for physicians. Recent research indicates that cell metabolism plays important roles in the pathogenesis of rheumatic diseases. In this review, we mainly focus on lipid metabolism profiles (dyslipidaemia, fatty acid metabolism) and mechanisms in rheumatic diseases and discuss potential clinical applications based on lipid metabolism profiles.

scholarly journals Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinghui Lu ◽  
Yankun Zhang ◽  
Min Sun ◽  
Changyuan Ding ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Thyroid Carcinoma ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Metabolic Reprogramming ◽  
Clinical Samples ◽  
Integrated Analysis ◽  
Additional Energy

ObjectivePapillary thyroid carcinoma (PTC) accounts for the majority of thyroid cancer and affects a large number of individuals. The pathogenesis of PTC has not been completely elucidated thus far. Metabolic reprogramming is a common feature in tumours. Our previous research revealed the reprogramming of lipid metabolism in PTC. Further studies on lipid metabolism reprogramming may help elucidate the pathogenesis of PTC.MethodsClinical samples of PTC and para-tumour tissue were analysed using lipidomic, proteomic, and metabolomic approaches. A multi-omics integrative strategy was adopted to identify the important pathways in PTC. The findings were further confirmed using western blotting, tissue microarray, bioinformatics, and cell migration assays.ResultsMulti-omics data and the results of integrated analysis revealed that the three steps of fatty acid metabolism (hydrolysis, transportation, and oxidation) were significantly enhanced in PTC. Especially, the expression levels of LPL, FATP2, and CPT1A, three key enzymes in the respective steps, were elevated in PTC. Moreover, LPL, FATP2 and CPT1A expression was associated with the TNM stage, lymph node metastasis of PTC. Moreover, high levels of FATP2 and CPT1A contributed to poor prognosis of PTC. In addition, ectopic overexpression of LPL, FATP2 and CPT1A can each promote the migration of thyroid cancer cells.ConclusionsOur data suggested that enhanced fatty acid metabolism supplied additional energy and substrates for PTC progression. This may help elucidating the underlying mechanism of PTC pathogenesis and identifying the potential therapeutic targets for PTC.

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