scholarly journals Effect of Mycorrhizal Inoculation and Irrigation on Biological Properties of Sweet Pepper Rhizosphere in Organic Field Cultivation

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1693
Author(s):  
Agnieszka Jamiołkowska ◽  
Barbara Skwaryło-Bednarz ◽  
Elżbieta Patkowska ◽  
Halina Buczkowska ◽  
Anna Gałązka ◽  
...  
Keyword(s):  
Catalase Activity ◽  
Mycorrhizal Fungi ◽  
Dna Analysis ◽  
Sweet Pepper ◽  
Biological Properties ◽  
Molecular Techniques ◽  
Soil Dna ◽  
Biolog Ecoplates ◽  
Mycorrhizal Inoculation ◽  
Physiological Profiles

The aim of the study was to evaluate the influence of mycorrhizal fungi (MF) and irrigation on biological properties of sweet pepper rhizosphere in organic field cultivation. For this purpose, MF were applied to plants in the form of commercial mycorrhizal inoculum (Rhizophagus aggregatus, R. intraradices, Claroideoglomus etunicatum, Endogone mosseae, Funneliformis caledonium, and Gigaspora margarita) and irrigation according to the combinations: mycorrhized plants (PM), mycorrhized and irrigated plants (PMI), and irrigated plants (PI). Plants without MF and irrigation served as the absolute control (P). The study used classic and molecular techniques, assessing catalase activity, biodiversity of soil microorganisms (soil DNA analysis), and the Community-Level Physiological Profiles (CLPP) analysis using Biolog EcoPlates. The highest catalase activity was recorded in the control and mycorrhized soil sample. The highest total number of bacteria was noted in the rhizosphere of control plants (P) and irrigated plants, while the lowest number in the rhizosphere of mycorrhized and irrigated plants. Plant irrigation contributed to the increase in the total number of fungi in the rhizosphere. The rhizospheric soil of PM and PMI were characterized by the highest utilization of amines, amides, and amino acids, whereas the lowest level of utilization was detected in the P and PI rhizospheres. The highest biodiversity and metabolic activity were observed in the rhizospheres from the PMI and PM samples, whereas lower catabolic activity were recorded in the P and PI rhizospheres. The mycorrhization of crops improved the biological properties of the rhizosphere, especially under conditions of drought stress.

scholarly journals EFFECT OF MYCORRHIZA INOCULATION OF PEPPER SEEDLINGS (Capsicum annuum L.) ON THE GROWTH AND PROTECTION AGAINST Fusarium oxysporum INFECTION

2019 ◽  
Vol 18 (1) ◽  
pp. 161-169
Author(s):  
Agnieszka Jamiołkowska ◽  
Władysław Michałek
Keyword(s):  
Fusarium Oxysporum ◽  
Root Rot ◽  
Mycorrhizal Fungi ◽  
Photosynthetic Activity ◽  
Pathogenic Fungus ◽  
Arbuscular Mycorrhizal ◽  
Sweet Pepper ◽  
Disease Index ◽  
Mycorrhizal Inoculation ◽  
And Control

A study was conducted to investigate the ability of arbuscular mycorrhizal fungi (AMF) in enhancing the growth and control of a root rot caused by different isolates of Fusarium oxysporum in sweet pepper seedlings. The plants were grown in plastic pots filled with sterilized horticultural soils. There were four treatments applied as follows: Fo (seedlings infected with fungus), M (seedlings inoculated with mycorrhizal fungi), Fo + M (seedlings inoculated with mycorrhiza and infected with fungus) and control. A randomized experiment was used and the growth, disease index, and photosynthetic activity of the plants were measured after 4 weeks. The investigations showed that the mycorrhizal inoculation had a protective effect on the F. oxysporum-infected pepper seedlings. The plants were characterized by a higher growth rate and a lower disease index than those growing only in the presence of the pathogenic fungus. The roots inoculated with mycorrhizal fungi were better developed than those infected only with F. oxysporum. The mycorrhiza contributed to an increase in the photosynthetic activity of the pepper seedlings.

scholarly journals The Effects of Inoculation with Three Glomus Species on Growth and Pb Uptake by Hemp (Cannabis Sativa) in a Pb-Contaminated Soil

2020 ◽  
pp. 1-15
Author(s):  
Roya Zalaghi ◽  
Ali-Akbar Safari-Sinegani ◽  
Nasser Aliasgharzad
Keyword(s):  
Mycorrhizal Fungi ◽  
Cannabis Sativa ◽  
Translocation Factor ◽  
Biological Properties ◽  
Glomus Species ◽  
Mycorrhizal Inoculation ◽  
Living Organisms ◽  
Water Ecosystems ◽  
Suitable Index ◽  
Pb Concentration

Increasing levels of hazardous heavy metals, which enter soil and water ecosystems have had a detrimental effect on global living organisms and needs special attention. The effect of inoculation with three Glomus mycorrhizal fungi (G. mosseae, G. etunicatum and G. constrictum) on hemp growth and some biological properties was evaluated in a soil contaminated with different levels of lead (0, 600, 1200 and 1800mg·kg-1 Pb soil) in the form of Pb(NO3)2. The results show that shoot and root dry weights decreased with increasing Pb levels in soil. But Pb concentration in roots and shoots of hemp increased significantly with increasing Pb levels in soil. However the growth of mycorrhizal inoculated hemps was significantly higher than that of non-inoculated hemp. Mycorrhizal inoculation increased the concentration of Pb in the shoot and root of hemp. In this study, both translocation factor (TF) and enrichment factor (EF) were < 1 and decreased with increasing Pb concentration in soil. The TF was higher in non-inoculated hemp than inoculated hemps. It shows the inhibition effect of mycorrhiza in translocation of Pb from root to shoot. The EF of all inoculated hemps (0.18) was significantly greater than non-inoculated hemp (0.17), showing increased uptake of Pb due to inoculation with mycorrhiza. Simple linear regressions show that the diethylenetriaminepentaacetic acid (DTPA) extractable Pb is more suitable index for evaluating the toxicity effect of soil Pb than the total Pb content.

Effects of arbuscular mycorrhizal fungi onCapsicumspp.

2015 ◽  
Vol 154 (5) ◽  
pp. 828-849 ◽  
Author(s):  
J. A. P. PEREIRA ◽  
I. J. C. VIEIRA ◽  
M. S. M. FREITAS ◽  
C. L. PRINS ◽  
M. A. MARTINS ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Growth Yield ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Sweet Pepper ◽  
Economic Importance ◽  
Growth And Yield ◽  
Plant Interaction ◽  
Mycorrhizal Inoculation

SUMMARYThe benefits of mycorrhizal inoculation on growth, yield and nutrition of plants are well documented. However, mycorrhiza use in pepper and sweet pepper crops (Capsicumspp.) is still rarely exploited compared to other crops of economic importance. The current paper reviews the main aspects of the association between arbuscular mycorrhizal (AM) fungi and plants of pepper and sweet pepper. It includes topics about the effects of AM fungi on nutrition, growth and yield inCapsicumspp., paying particular attention to AM fungi–pathogen interactions, responses to some environmental stresses, as well as biochemical and physiological aspects of AM fungi–plant interaction inCapsicum annuumL.

scholarly journals First Insights into the Effect of Mycorrhizae on the Expression of Pathogen Effectors during the Infection of Grapevine with Plasmopara viticola

2021 ◽  
Vol 13 (3) ◽  
pp. 1226
Author(s):  
Ana Cruz-Silva ◽  
Andreia Figueiredo ◽  
Mónica Sebastiana
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Plasmopara Viticola ◽  
Vitis Vinifera L ◽  
Wine Production ◽  
Grapevine Downy Mildew ◽  
Pathogen Effectors ◽  
Rxlr Effectors ◽  
Mycorrhizal Inoculation ◽  
And Control

Grapevine (Vitis vinifera L.), widely used for berry and wine production, is highly susceptible to the pathogenic oomycete Plasmopara viticola, the etiological agent of grapevine downy mildew disease. The method commonly used to prevent and control P. viticola infection relies on multiple applications of chemical fungicides. However, with European Union goals to lower the usage of such chemicals in viticulture there is a need to develop new and more sustainable strategies. The use of beneficial microorganisms with biocontrol capabilities, such as the arbuscular mycorrhizal fungi (AMF), has been pointed out as a viable alternative. With this study, we intended to investigate the effect of AMF colonization on the expression of P. viticola effectors during infection of grapevine. Grapevine plants were inoculated with the AMF Rhizophagus irregularis and, after mycorrhizae development, plants were infected with P. viticola. The expression of P. viticola RxLR effectors was analyzed by real-time PCR (qPCR) during the first hours of interaction. Results show that pre-mycorrhizal inoculation of grapevine alters the expression of several P. viticola effectors; namely, PvRxLR28, which presented decreased expression in mycorrhizal plants at the two time points post-infection tested. These results suggest that the pre-inoculation of grapevine with AMF could interfere with the pathogen’s ability to infect grapevine by modulation of pathogenicity effectors expression, supporting the hypothesis that AMF can be used to increase plant resistance to pathogens and promote more sustainable agriculture practices, particularly in viticulture.

scholarly journals Influence of Pollution by Antibiotics on Biological Properties of Soils (Through the Example of Ordinary Chernozem)

2021 ◽  
Author(s):  
Yuliya Akimenko
Keyword(s):  
Biological Activity ◽  
Polyphenol Oxidase ◽  
Catalase Activity ◽  
Direct Relationship ◽  
Negative Impact ◽  
Biological Indicators ◽  
Biological Properties ◽  
Field Conditions ◽  
Ordinary Chernozem ◽  
Biological Parameters

Abstract In model laboratory and field conditions, the influence of pollution by antibiotics (benzylpenicillin, ampicillin, streptomycin, oxytetracycline, tylosin, pharmasin, tromexin, aliseryl, and nystatin) on the biological properties of ordinary chernozem was examined in concentrations of 1-1000 mg/kg. A decrease in the majority of the basic biological parameters of chernozem occurs when the concentration of antibiotics is 100 mg/kg of soil. In most cases, there was a direct relationship between the content of antibiotics in the soil and the scale of a decrease in the studied parameters. The degree of the influence of antibiotics was determined by their nature, concentration and time of exposure. Antibacterial antibiotics had more negative impact on the studied indicators than fungicidal ones. By the degree of inhibiting the biological properties of chernozem, antibiotics formed the following sequence: ampicillin > benzylpenicillin ≥ streptomycin ≥ oxytetracycline > tylosin ≥ pharmasin > nystatin > tromexin > aliseryl. Among the examined biological parameters when polluted by antibiotics, the most informative one was the number of ammonifying bacteria and the activity of dehydrogenases. The least informative was the indicator of catalase activity. The abundance of bacteria of the genus Azotobacter in case of pollution by antibiotics was not informative. The degree of a decrease in biological indicators was more pronounced in laboratory conditions than in the field ones. The rate of the biological activity recovery of chernozem after pollution in the field was 2 times higher. According to the degree of resistance to antibiotics, the investigated microorganisms of chernozem formed the following sequence: bacteria of the genus Azotobacter > micromycetes > amylolytic bacteria > ammonifying bacteria. Enzymes formed the following sequence: peroxidase ≥ polyphenol oxidase > catalase > dehydrogenase > invertase ≥ phosphatase. Antibiotics had prolonged influence on the biological properties of ordinary chernozem. The examined parameters were observed not to be recovered to control values even on the 120th day after the pollution.

scholarly journals Mycorrhizal Inoculation and Chemical Fertilizer Interactions in Pineapple under Field Conditions

Agriculture ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 934
Author(s):  
Dora Trejo ◽  
Wendy Sangabriel-Conde ◽  
Mayra E. Gavito-Pardo ◽  
Jacob Banuelos
Keyword(s):  
Fruit Quality ◽  
Mycorrhizal Fungi ◽  
Block Design ◽  
Chemical Fertilizer ◽  
Production Costs ◽  
Partial Substitution ◽  
Experimental Unit ◽  
Chemical Fertilization ◽  
Mycorrhizal Inoculation ◽  
Amf Inoculation

Excessive inorganic fertilizers applied to pineapple crops in Mexico cause the progressive degradation and pollution of soils in the short- and long-term, and they also increase production costs. An alternative to reduce excessive fertilization is its partial substitution by nutrition and growth enhancing arbuscular mycorrhizal fungi (AMF). The goal of this research was to compare the effect of AMF inoculation combined with different fertilizer doses and full chemical fertilization on pineapple yield variables in a commercial plantation. We used a randomized block design with six treatments: a non-inoculated control with 100% chemical fertilization, and five treatments with AMF inoculation and fertilization doses of 0%, 25%, 50%, 75%, and 100% chemical fertilization. There were four replicates of each treatment containing 30 plants in each experimental unit (plot). We measured the dry weight of the D-leaf 9 months after planting, and the root mycorrhizal colonization percentage, yield, and fruit quality after 18 months. Mycorrhizal inoculation equated to 100% chemical fertilization already when combined with 25% fertilization and surpassed it when combined with 50% fertilization in most of the yield variables measured. The fruit mass and organoleptic variables were significantly higher in mycorrhizal plants with 50% fertilization than in the non-inoculated control and the treatments inoculated with AMF and combined with 0%, 25%, 75%, and 100% of a dose of chemical fertilizer. Inoculation with mycorrhizal fungi in the field could reduce chemical fertilizer application by 50%, with no yield loss and with improved fruit quality.

Mycorrhizal inoculation and application of Fe correction in the soil for ameliorating grapevine performance in a copper-contaminated soil

2021 ◽  
Author(s):  
Amaia Nogales ◽  
Erika S. Santos ◽  
Gonçalo Victorino ◽  
Wanda Viegas ◽  
Maria Manuela Abreu
Keyword(s):  
Soil Contamination ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Mycorrhizal Fungi ◽  
Root Biomass ◽  
Arbuscular Mycorrhizal ◽  
Nutrient Contents ◽  
Beneficial Effects ◽  
Continuous Application ◽  
Mycorrhizal Inoculation

&lt;p&gt;Copper-based fungicides are commonly applied in vineyards to control fungal diseases that can severely affect grapevine productivity. Continuous application of this type of fungicides contributes to Cu accumulation in surface horizons of the soil, which can generate toxicity problems in plants, regardless of being an essential nutrient. Several strategies have been proposed to immobilize or counteract the effect of soil contaminants, such as plant inoculation with arbuscular mycorrhizal fungi (AMF). However, depending on the element concentration, this may not be sufficient to avoid its excessive accumulation in belowground and/or aboveground organs. Since Fe is known to have an antagonistic interaction with Cu in plants, Fe application, as an amendment, in vineyard soils, could be a good strategy to avoid excessive Cu uptake by grapevines growing in Cu-contaminated soils. However, little information is available on the combined effects of both strategies.&lt;/p&gt;&lt;p&gt;In order to reveal the possible beneficial effects of plant mycorrhization and Fe application in Cu-contaminated soils on grapevine growth and nutrition, a mesocosm experiment was established under controlled conditions. Two-year-old plants, previously inoculated or not with two different AMF, were grown in pots filled with 6.5 kg of an Arenosol collected from a wine-growing region. These plants were subjected to three soil treatments: 1) soil contamination with Cu, where the grapevines were watered with a solution containing 5.89 mg/L CuSO&lt;sub&gt;4&lt;/sub&gt; to ensure that the soil in each container reached 300 mg Cu/kg; 2) soil contamination with Cu + Fe addition, where the plants were watered with a solution that contained the same amount of CuSO&lt;sub&gt;4&lt;/sub&gt; plus 0.38 mg/L of FeNaEDTA&amp;#183;3H&lt;sub&gt;2&lt;/sub&gt;O to achieve 100 mg of Fe/kg soil; and 3) non-contaminated soil watered with deionized water. Four months later, at the end of the growing season, plant vegetative growth as well as leaf and root nutrient contents were analyzed.&lt;/p&gt;&lt;p&gt;Grapevines inoculated with AMF demonstrated a good level of tolerance to high Cu concentrations in soil, as they presented significantly higher root biomass than non-inoculated plants and Cu was mainly accumulated in the roots avoiding its translocation to the aerial part. However, when the Cu-contaminated soil was amended with Fe, a significant decrease was observed in root biomass in all mycorrhizal inoculation treatments and Cu was accumulated in grapevine leaves. Contrastingly, Fe application helped to avoid the excessive increase of Mn concentrations in leaf and roots that is commonly induced in Cu contaminated soils, which can be detrimental for grapevine growth.&lt;/p&gt;&lt;p&gt;These results demonstrated that mycorrhizal inoculation is a suitable strategy to promote grapevine growth in Cu-contaminated soils. However, special attention needs to be taken when applying amendments to correct Cu contamination, as the mycorrhizal status of plants may alter the expected outcome.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;div&gt; &lt;div&gt;&amp;#160;&lt;/div&gt; &lt;/div&gt;

scholarly journals Effect of Arbuscular Mycorrhizal Fungi on the Tolerance to Sodium Chloride Levels, and on Growth and Yield of Lentil (Lens culinaris)

2017 ◽  
Vol 15 (1) ◽  
pp. 156-169 ◽  
Author(s):  
M Rahman ◽  
MAH Bhuiyan ◽  
ME Ali ◽  
F Alam ◽  
RA Begum
Keyword(s):  
Sodium Chloride ◽  
Seed Yield ◽  
Mycorrhizal Fungi ◽  
Agricultural Research ◽  
Block Design ◽  
Growth And Yield ◽  
Yield Attributes ◽  
Stover Yield ◽  
Mycorrhizal Inoculation ◽  
Mycorrhizal Plants

A pot experiment was carried out in the nethouse of Soil Science Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur during 2014-2015 through 2015-2016. The design of the experiment was factorial randomized completely block design with 4 replications. The objectives of the study were to evaluate the potential of Arbuscular mycorrhization (AM) on the germination, yield and yield attributes of lentil treated with different concentration of sodium chloride (NaCl). Five NaCl treatments (0, 1, 2, 3 and 4%) possessed NaCl level as the first factor that were treated with soils before sowing of lentil seeds overriding or pivotal pulse crop in Bangladesh. The second factor consists of mycorrhizal and non-mycorrhizal treatments. Mycorrhizal plants showed better performance in terms of germination %, yield and yield contributing characters than non-mycorrhizal plants. With increasing NaCl concentration germination %, yield and yield contributing characters in the rhizosphere soil, decreased significantly (p<0.01). Interaction effects of mycorrhizal inoculation and NaCl on germination %, growth and yield of lentil were appeared to be statistically non-significant. The highest germination (96.25% in 2014-2015 and 92.50% in 2015-2016), seed yield (6.45 g pot-1 in 2014-2015 and 5.89 g pot-1 in 2015-2016), and stover yield (9.55 g pot-1 in 2014-2015 and 8.58 g pot-1 in 2015-2016) was found in NaCl 0% + AM treatment. The lowest germination %, seed yield and stover yield was found in NaCl 4% treatment. Mycorrhizal inoculation increased seed yield on an average by 31.85% during 2014-2015 and 63.71% during 2015-2016, and increased stover yield on an average by 48.56% during 2014-2015 and 63.55% during 2015-2016 over non-mycorrhizal inoculation. Therefore, it can be concluded that mycorrhizal inoculation increases germination %, growth and yield of lentil over non-mycorrhizal inoculation. The Agriculturists 2017; 15(1) 156-169

scholarly journals Arbuscular mycorrhizal fungal communities and soil aggregation as affected by cultivation of various crops during the sugarcane fallow period

2014 ◽  
Vol 38 (2) ◽  
pp. 415-422 ◽  
Author(s):  
Priscila Viviane Truber ◽  
Carolina Fernandes
Keyword(s):  
Cover Crops ◽  
Mycorrhizal Fungi ◽  
Block Design ◽  
Aggregate Stability ◽  
Arbuscular Mycorrhizal ◽  
Biological Properties ◽  
Soil Aggregation ◽  
Soil Tillage ◽  
Fallow Period ◽  
Red Latosol

Management systems involving crop rotation, ground cover species and reduced soil tillage can improve the soil physical and biological properties and reduce degradation. The primary purpose of this study was to assess the effect of various crops grown during the sugarcane fallow period on the production of glomalin and arbuscular mycorrhizal fungi in two Latosols, as well as their influence on soil aggregation. The experiment was conducted on an eutroferric Red Latosol with high-clay texture (680 g clay kg-1) and an acric Red Latosol with clayey texture (440 g kg-1 clay) in Jaboticabal (São Paulo State, Brazil). A randomized block design involving five blocks and four crops [soybean (S), soybean/fallow/soybean (SFS), soybean/millet/soybean (SMS) and soybean/sunn hemp/soybean (SHS)] was used to this end. Soil samples for analysis were collected in June 2011. No significant differences in total glomalin production were detected between the soils after the different crops. However, total external mycelium length was greater in the soils under SMS and SHS. Also, there were differences in easily extractable glomalin, total glomalin and aggregate stability, which were all greater in the eutroferric Red Latosol than in the acric Red Latosol. None of the cover crops planted in the fallow period of sugarcane improved aggregate stability in either Latosol.

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