scholarly journals Systematic Investigations of the ZF-HD Gene Family in Tobacco Reveal Their Multiple Roles in Abiotic Stresses

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 406
Author(s):  
Jinhao Sun ◽  
Minmin Xie ◽  
Xiaoxu Li ◽  
Zhiyuan Li ◽  
Qi Wang ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Gene Silencing ◽  
Abiotic Stresses ◽  
Multiple Roles ◽  
Virus Induced Gene Silencing ◽  
Nicotiana Tabacum L ◽  
Localization Analysis ◽  
Gene Structures ◽  
Functional Analyses ◽  
Profiling Analysis

Zinc finger homeodomain (ZF-HD) transcription factors play significant roles in plant growth and responses to environmental stresses. In this study, 32 ZF-HD genes identified in the tobacco (Nicotiana tabacum L.) genome were divided into six groups according to phylogenetic analysis with Arabidopsis and tomato ZF-HD members. An examination of gene structures and conserved motifs revealed the relatively conserved exon/intron structures and motif organization within each subgroup. In addition, various stress-related elements are found in the promoter region of these genes. The expression profiling analysis revealed that NtZF-HD genes expressed in different tissues and could be induced by several abiotic stresses. Notably, NtZF-HD21 was highly expressed in response to the drought treatments. Subcellular localization analysis and a virus-induced gene silencing (VIGS) experiment were performed to investigate the potential functions of NtZF-HD21. The subcellular localization indicated that NtZF-HD21 is a nuclear protein. Furthermore, gene silencing of the NtZF-HD21 gene reduced the drought resistance of tobacco. These findings provide insights for further biological functional analyses of the NtZF-HD genes in tobacco.

scholarly journals Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean

2021 ◽  
Vol 22 (20) ◽  
pp. 11032
Author(s):  
Jamie A. O’Rourke ◽  
Michael J. Morrisey ◽  
Ryan Merry ◽  
Mary Jane Espina ◽  
Aaron J. Lorenz ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Iron Deficiency ◽  
Gene Silencing ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Yield Loss ◽  
Abiotic Stress Tolerance ◽  
Iron Stress ◽  
Rna Seq ◽  
Virus Induced Gene Silencing

The soybean (Glycine max L. merr) genotype Fiskeby III is highly resistant to a multitude of abiotic stresses, including iron deficiency, incurring only mild yield loss during stress conditions. Conversely, Mandarin (Ottawa) is highly susceptible to disease and suffers severe phenotypic damage and yield loss when exposed to abiotic stresses such as iron deficiency, a major challenge to soybean production in the northern Midwestern United States. Using RNA-seq, we characterize the transcriptional response to iron deficiency in both Fiskeby III and Mandarin (Ottawa) to better understand abiotic stress tolerance. Previous work by our group identified a quantitative trait locus (QTL) on chromosome 5 associated with Fiskeby III iron efficiency, indicating Fiskeby III utilizes iron deficiency stress mechanisms not previously characterized in soybean. We targeted 10 of the potential candidate genes in the Williams 82 genome sequence associated with the QTL using virus-induced gene silencing. Coupling virus-induced gene silencing with RNA-seq, we identified a single high priority candidate gene with a significant impact on iron deficiency response pathways. Characterization of the Fiskeby III responses to iron stress and the genes underlying the chromosome 5 QTL provides novel targets for improved abiotic stress tolerance in soybean.

scholarly journals Genome-Wide Identification and Expression Profiling Analysis of the Trihelix Gene Family Under Abiotic Stresses in Medicago truncatula

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1389
Author(s):  
Xiqiang Liu ◽  
Han Zhang ◽  
Lin Ma ◽  
Zan Wang ◽  
Kun Wang
Keyword(s):  
Medicago Truncatula ◽  
Abiotic Stresses ◽  
Growth And Development ◽  
Chromosomal Distribution ◽  
Specific Expression ◽  
Genome Wide ◽  
Duplication Events ◽  
Gene Structures ◽  
Syntenic Analysis ◽  
Profiling Analysis

The trihelix transcription factor (GT) family is widely involved in regulating plant growth and development, and most importantly, responding to various abiotic stresses. Our study first reported the genome-wide identification and analysis of GT family genes in Medicago truncatula. Overall, 38 trihelix genes were identified in the M. truncatula genome and were classified into five subfamilies (GT-1, GT-2, SH4, GTγ and SIP1). We systematically analyzed the phylogenetic relationship, chromosomal distribution, tandem and segmental duplication events, gene structures and conserved motifs of MtGTs. Syntenic analysis revealed that trihelix family genes in M. truncatula had the most collinearity relationship with those in soybean followed by alfalfa, but very little collinearity with those in the maize and rice. Additionally, tissue-specific expression analysis of trihelix family genes suggested that they played various roles in the growth and development of specific tissues in M. truncatula. Moreover, the expression of some MtGT genes, such as MtGT19, MtGT20, MtGT22, and MtGT33, was dramatically induced by drought, salt, and ABA treatments, illustrating their vital roles in response to abiotic stresses. These findings are helpful for improving the comprehensive understanding of trihelix family; additionally, the study provides candidate genes for achieving the genetic improvement of stress resistance in legumes.

scholarly journals Functional analyses of cellulose synthase genes in flax (Linum usitatissimum) by virus-induced gene silencing

2015 ◽  
Vol 13 (9) ◽  
pp. 1312-1324 ◽  
Author(s):  
Maxime Chantreau ◽  
Brigitte Chabbert ◽  
Sylvain Billiard ◽  
Simon Hawkins ◽  
Godfrey Neutelings
Keyword(s):  
Gene Silencing ◽  
Linum Usitatissimum ◽  
Cellulose Synthase ◽  
Virus Induced Gene Silencing ◽  
Functional Analyses

Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (12) ◽  
Author(s):  
Lokanadha Gunupuru ◽  
Shahin Ali ◽  
Fiona Doohan ◽  
Steven Scofield
Keyword(s):  
Gene Silencing ◽  
Virus Induced Gene Silencing ◽  
Barley Seedling

scholarly journals Cucumber mosaic virus-induced gene silencing in banana

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuh Tzean ◽  
Ming-Chi Lee ◽  
Hsiao-Hsuan Jan ◽  
Yi-Shu Chiu ◽  
Tsui-Chin Tu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Induced Gene Silencing

scholarly journals Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in Dendrobium catenatum

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1204
Author(s):  
Xinqiao Zhan ◽  
Yichun Qian ◽  
Bizeng Mao
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Expression Profiling ◽  
Abiotic Stresses ◽  
Membrane Lipids ◽  
Chinese Herb ◽  
Growth Stages ◽  
Limited Information ◽  
Gene Structures ◽  
Functional Investigation

Dendrobium catenatum, a valuable Chinese herb, frequently experiences abiotic stresses, such as cold and drought, under natural conditions. Nonphosphorus glycerolipid synthase (NGLS) genes are closely linked to the homeostasis of membrane lipids under abiotic stress in plants. However, there is limited information on NGLS genes in D. catenatum. In this study, a total of eight DcaNGLS genes were identified from the D. catenatum genome; these included three monogalactosyldiacylglycerol synthase (DcaMGD1, 2, 3) genes, two digalactosyldiacylglycerol synthase (DcaDGD1, 2) genes, and three sulfoquinovosyldiacylglycerol synthase (DcaSQD1, 2.1, 2.2) genes. The gene structures and conserved motifs in the DcaNGLSs showed a high conservation during their evolution. Gene expression profiling showed that the DcaNGLSs were highly expressed in specific tissues and during rapid growth stages. Furthermore, most DcaNGLSs were strongly induced by freezing and post-freezing recovery. DcaMGD1 and DcaSQDs were greatly induced by salt stress in leaves, while DcaDGDs were primarily induced by salt stress in roots. Under drought stress, most DcaNGLSs were regulated by circadian rhythms, and DcaSQD2 was closely associated with drought recovery. Transcriptome analysis also revealed that MYB might be regulated by circadian rhythm and co-expressed with DcaNGLSs under drought stress. These results provide insight for the further functional investigation of NGLS and the regulation of nonphosphorus glycerolipid biosynthesis in Dendrobium.

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